Restriction map for
pF1KA0879
(Linear) (Six-base) MAPSORT of: KIAA0879.seq Check: 6832 from: 1 to: 1359
KIAA0879.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 142 358 611 1306 1359
Size: 90 52 216 253 695 53
Fragments arranged by size:
695 253 216 90 53 52
AhdI GACnn_n'nnGTC
Cuts at: 0 1166 1359
Size: 1166 193
AlwNI CAG_nnn'CTG
Cuts at: 0 805 1359
Size: 805 554
ApoI r'AATT_y
Cuts at: 0 133 150 929 1359
Size: 133 17 779 430
Fragments arranged by size:
779 430 133 17
AseI AT'TA_AT
Cuts at: 0 1205 1359
Size: 1205 154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 404 437 1359
Size: 404 33 922
Fragments arranged by size:
922 404 33
BamHI G'GATC_C
Cuts at: 0 324 1359
Size: 324 1035
BanII G_rGCy'C
Cuts at: 0 799 1359
Size: 799 560
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 1359
Size: 896 463
BciVI GTATCCnnnnn_n'
Cuts at: 0 102 1359
Size: 102 1257
BclI T'GATC_A
Cuts at: 0 695 708 1359
Size: 695 13 651
Fragments arranged by size:
695 651 13
BfrBI ATG'CAT
Cuts at: 0 1035 1359
Size: 1035 324
BmrI ACTGGGnnnn_n'
Cuts at: 0 717 793 1359
Size: 717 76 566
Fragments arranged by size:
717 566 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1359
Size: 130 1229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1359
Size: 646 713
BsaBI GATnn'nnATC
Cuts at: 0 1109 1231 1343 1359
Size: 1109 122 112 16
Fragments arranged by size:
1109 122 112 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 1359
Size: 456 903
Bsp1286I G_dGCh'C
Cuts at: 0 799 1359
Size: 799 560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1068 1359
Size: 1068 291
BstBI TT'CG_AA
Cuts at: 0 520 1359
Size: 520 839
BstEII G'GTnAC_C
Cuts at: 0 360 1000 1359
Size: 360 640 359
Fragments arranged by size:
640 360 359
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1359
Size: 945 414
BstYI r'GATC_y
Cuts at: 0 324 1359
Size: 324 1035
BtgI C'CryG_G
Cuts at: 0 255 1005 1053 1359
Size: 255 750 48 306
Fragments arranged by size:
750 306 255 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1206 1359
Size: 1206 153
ClaI AT'CG_AT
Cuts at: 0 764 1359
Size: 764 595
EaeI y'GGCC_r
Cuts at: 0 573 1359
Size: 573 786
EarI CTCTTCn'nnn_
Cuts at: 0 63 447 1359
Size: 63 384 912
Fragments arranged by size:
912 384 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 130 142 358 611 1306 1359
Size: 90 40 12 216 253 695 53
Fragments arranged by size:
695 253 216 90 53 40 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1160 1359
Size: 1160 199
EcoRI G'AATT_C
Cuts at: 0 929 1359
Size: 929 430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 74 318 1359
Size: 74 244 1041
Fragments arranged by size:
1041 244 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 424 456 1152 1184 1359
Size: 46 32 346 32 696 32 175
Fragments arranged by size:
696 346 175 46 32 32 32
HincII GTy'rAC
Cuts at: 0 1191 1253 1359
Size: 1191 62 106
Fragments arranged by size:
1191 106 62
HpaI GTT'AAC
Cuts at: 0 1253 1359
Size: 1253 106
MfeI C'AATT_G
Cuts at: 0 968 1359
Size: 968 391
MscI TGG'CCA
Cuts at: 0 575 1359
Size: 575 784
MslI CAynn'nnrTG
Cuts at: 0 872 1010 1359
Size: 872 138 349
Fragments arranged by size:
872 349 138
NcoI C'CATG_G
Cuts at: 0 255 1005 1359
Size: 255 750 354
Fragments arranged by size:
750 354 255
NdeI CA'TA_TG
Cuts at: 0 869 1359
Size: 869 490
NruI TCG'CGA
Cuts at: 0 1225 1359
Size: 1225 134
NsiI A_TGCA'T
Cuts at: 0 1037 1359
Size: 1037 322
NspI r_CATG'y
Cuts at: 0 184 1268 1359
Size: 184 1084 91
Fragments arranged by size:
1084 184 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 1131 1359
Size: 1131 228
PpuMI rG'GwC_Cy
Cuts at: 0 1160 1359
Size: 1160 199
PsiI TTA'TAA
Cuts at: 0 37 842 1359
Size: 37 805 517
Fragments arranged by size:
805 517 37
SfcI C'TryA_G
Cuts at: 0 222 858 1359
Size: 222 636 501
Fragments arranged by size:
636 501 222
SmlI C'TyrA_G
Cuts at: 0 661 1359
Size: 661 698
SpeI A'CTAG_T
Cuts at: 0 87 1359
Size: 87 1272
SspI AAT'ATT
Cuts at: 0 498 1359
Size: 498 861
StyI C'CwwG_G
Cuts at: 0 255 1005 1359
Size: 255 750 354
Fragments arranged by size:
750 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 348 1359
Size: 348 1011
Tth111I GACn'n_nGTC
Cuts at: 0 649 1359
Size: 649 710
XmnI GAAnn'nnTTC
Cuts at: 0 893 1359
Size: 893 466
Enzymes that do cut:
AcuI AhdI AlwNI ApoI AseI BaeI BamHI BanII
BbsI BciVI BclI BfrBI BmrI BpmI BpuEI BsaBI
BseRI Bsp1286I BspMI BstBI BstEII BstXI BstYI BtgI
BtgZI ClaI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII HpaI MfeI MscI MslI NcoI NdeI
NruI NsiI NspI PflMI PpuMI PsiI SfcI SmlI
SpeI SspI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BanI BbeI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstZ17I Bsu36I BtsI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HindIII KasI KpnI MluI MmeI
MspA1I NaeI NarI NgoMIV NheI NotI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI TatI
TstI XbaI XcmI XhoI XmaI ZraI