Restriction map for
pF1KSDA0880
(Linear) (Six-base) MAPSORT of: KIAA0880.seq Check: 4123 from: 1 to: 2127
KIAA0880.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 969 2127
Size: 969 1158
AatII G_ACGT'C
Cuts at: 0 259 2127
Size: 259 1868
Acc65I G'GTAC_C
Cuts at: 0 30 39 2127
Size: 30 9 2088
Fragments arranged by size:
2088 30 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1350 2127
Size: 907 443 777
Fragments arranged by size:
907 777 443
AfeI AGC'GCT
Cuts at: 0 235 2127
Size: 235 1892
AgeI A'CCGG_T
Cuts at: 0 1946 2127
Size: 1946 181
AleI CACnn'nnGTG
Cuts at: 0 1525 1876 2127
Size: 1525 351 251
Fragments arranged by size:
1525 351 251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 757 1657 1671 2127
Size: 349 408 900 14 456
Fragments arranged by size:
900 456 408 349 14
AlwNI CAG_nnn'CTG
Cuts at: 0 555 591 1984 2127
Size: 555 36 1393 143
Fragments arranged by size:
1393 555 143 36
ApaI G_GGCC'C
Cuts at: 0 1315 1890 2127
Size: 1315 575 237
Fragments arranged by size:
1315 575 237
ApaLI G'TGCA_C
Cuts at: 0 334 1879 2127
Size: 334 1545 248
Fragments arranged by size:
1545 334 248
AvaI C'yCGr_G
Cuts at: 0 679 2120 2127
Size: 679 1441 7
Fragments arranged by size:
1441 679 7
BamHI G'GATC_C
Cuts at: 0 683 1651 2127
Size: 683 968 476
Fragments arranged by size:
968 683 476
BanI G'GyrC_C
Cuts at: 0 30 39 609 820 1327 1683 2127
Size: 30 9 570 211 507 356 444
Fragments arranged by size:
570 507 444 356 211 30 9
BanII G_rGCy'C
Cuts at: 0 214 1000 1315 1714 1890 2127
Size: 214 786 315 399 176 237
Fragments arranged by size:
786 399 315 237 214 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 1068 1784 2127
Size: 1068 716 343
Fragments arranged by size:
1068 716 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 665 1845 1879 2127
Size: 631 34 1180 34 248
Fragments arranged by size:
1180 631 248 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1553 2127
Size: 1553 574
Bme1580I G_kGCm'C
Cuts at: 0 73 338 614 1315 1332 1437 1688 1883
Size: 73 265 276 701 17 105 251 195
Cuts at: 1883 1890 2127
Size: 7 237
Fragments arranged by size:
701 276 265 251 237 195 105 73
17 7
BmgBI CAC'GTC
Cuts at: 0 117 2127
Size: 117 2010
BmrI ACTGGGnnnn_n'
Cuts at: 0 1893 2127
Size: 1893 234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1494 2127
Size: 1494 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 231 1213 1380 1468 1945 2127
Size: 107 124 982 167 88 477 182
Fragments arranged by size:
982 477 182 167 124 107 88
Bpu10I CC'TnA_GC
Cuts at: 0 1891 2127
Size: 1891 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1545 2127
Size: 516 1029 582
Fragments arranged by size:
1029 582 516
BsaHI Gr'CG_yC
Cuts at: 0 256 1286 1902 2127
Size: 256 1030 616 225
Fragments arranged by size:
1030 616 256 225
BsaWI w'CCGG_w
Cuts at: 0 1946 2127
Size: 1946 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 1726 1756 2127
Size: 1186 30 510 30 371
Fragments arranged by size:
1186 510 371 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1778 2127
Size: 1778 349
BseYI C'CCAG_C
Cuts at: 0 501 548 594 729 831 1341 1516 1565
Size: 501 47 46 135 102 510 175 49
Cuts at: 1565 1837 2127
Size: 272 290
Fragments arranged by size:
510 501 290 272 175 135 102 49
47 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1689 2127
Size: 875 814 438
Fragments arranged by size:
875 814 438
BsiHKAI G_wGCw'C
Cuts at: 0 214 338 1883 2127
Size: 214 124 1545 244
Fragments arranged by size:
1545 244 214 124
Bsp1286I G_dGCh'C
Cuts at: 0 73 214 338 614 1000 1315 1332 1437
Size: 73 141 124 276 386 315 17 105
Cuts at: 1437 1688 1714 1883 1890 2127
Size: 251 26 169 7 237
Fragments arranged by size:
386 315 276 251 237 169 141 124
105 73 26 17 7
BspHI T'CATG_A
Cuts at: 0 188 392 2127
Size: 188 204 1735
Fragments arranged by size:
1735 204 188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 969 1238 1943 2127
Size: 969 269 705 184
Fragments arranged by size:
969 705 269 184
BsrBI CCG'CTC
Cuts at: 0 1377 2127
Size: 1377 750
BsrFI r'CCGG_y
Cuts at: 0 850 1946 2127
Size: 850 1096 181
Fragments arranged by size:
1096 850 181
BssSI C'ACGA_G
Cuts at: 0 1523 2127
Size: 1523 604
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2082 2127
Size: 2082 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1191 2127
Size: 1191 936
BstYI r'GATC_y
Cuts at: 0 683 1651 2056 2127
Size: 683 968 405 71
Fragments arranged by size:
968 683 405 71
Bsu36I CC'TnA_GG
Cuts at: 0 110 446 1816 2017 2127
Size: 110 336 1370 201 110
Fragments arranged by size:
1370 336 201 110 110
BtgI C'CryG_G
Cuts at: 0 1154 1851 2127
Size: 1154 697 276
Fragments arranged by size:
1154 697 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 828 2127
Size: 828 1299
BtsI GCAGTG_nn'
Cuts at: 0 701 863 2127
Size: 701 162 1264
Fragments arranged by size:
1264 701 162
ClaI AT'CG_AT
Cuts at: 0 641 2127
Size: 641 1486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1550 1585 2127
Size: 1550 35 542
Fragments arranged by size:
1550 542 35
EaeI y'GGCC_r
Cuts at: 0 121 1169 2127
Size: 121 1048 958
Fragments arranged by size:
1048 958 121
EarI CTCTTCn'nnn_
Cuts at: 0 1366 1384 2127
Size: 1366 18 743
Fragments arranged by size:
1366 743 18
EcoICRI GAG'CTC
Cuts at: 0 212 2127
Size: 212 1915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 231 907 1213 1350 1380 1468 1945
Size: 107 124 676 306 137 30 88 477
Cuts at: 1945 2127
Size: 182
Fragments arranged by size:
676 477 306 182 137 124 107 88
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1135 2127
Size: 1135 992
EcoO109I rG'GnC_Cy
Cuts at: 0 4 804 1312 1887 2127
Size: 4 800 508 575 240
Fragments arranged by size:
800 575 508 240 4
HaeII r_GCGC'y
Cuts at: 0 237 1200 1862 2127
Size: 237 963 662 265
Fragments arranged by size:
963 662 265 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 970 1002 2041 2073 2127
Size: 970 32 1039 32 54
Fragments arranged by size:
1039 970 54 32 32
KpnI G_GTAC'C
Cuts at: 0 34 43 2127
Size: 34 9 2084
Fragments arranged by size:
2084 34 9
MfeI C'AATT_G
Cuts at: 0 2080 2127
Size: 2080 47
MscI TGG'CCA
Cuts at: 0 1171 2127
Size: 1171 956
MslI CAynn'nnrTG
Cuts at: 0 557 577 1276 1525 1876 2127
Size: 557 20 699 249 351 251
Fragments arranged by size:
699 557 351 251 249 20
MspA1I CmG'CkG
Cuts at: 0 22 177 1619 2127
Size: 22 155 1442 508
Fragments arranged by size:
1442 508 155 22
NcoI C'CATG_G
Cuts at: 0 1154 2127
Size: 1154 973
PasI CC'CwG_GG
Cuts at: 0 1000 1082 1441 2127
Size: 1000 82 359 686
Fragments arranged by size:
1000 686 359 82
PflMI CCAn_nnn'nTGG
Cuts at: 0 781 1680 2127
Size: 781 899 447
Fragments arranged by size:
899 781 447
PfoI T'CCnGG_A
Cuts at: 0 1190 1574 2127
Size: 1190 384 553
Fragments arranged by size:
1190 553 384
PpuMI rG'GwC_Cy
Cuts at: 0 4 804 2127
Size: 4 800 1323
Fragments arranged by size:
1323 800 4
PspOMI G'GGCC_C
Cuts at: 0 1311 1886 2127
Size: 1311 575 241
Fragments arranged by size:
1311 575 241
PstI C_TGCA'G
Cuts at: 0 176 1097 1618 2127
Size: 176 921 521 509
Fragments arranged by size:
921 521 509 176
PvuII CAG'CTG
Cuts at: 0 177 1619 2127
Size: 177 1442 508
Fragments arranged by size:
1442 508 177
SacI G_AGCT'C
Cuts at: 0 214 2127
Size: 214 1913
SanDI GG'GwC_CC
Cuts at: 0 4 2127
Size: 4 2123
SapI GCTCTTCn'nnn_
Cuts at: 0 1384 2127
Size: 1384 743
SfcI C'TryA_G
Cuts at: 0 172 1093 1614 2127
Size: 172 921 521 513
Fragments arranged by size:
921 521 513 172
SmlI C'TyrA_G
Cuts at: 0 531 1560 2127
Size: 531 1029 567
Fragments arranged by size:
1029 567 531
StuI AGG'CCT
Cuts at: 0 716 1474 2127
Size: 716 758 653
Fragments arranged by size:
758 716 653
StyI C'CwwG_G
Cuts at: 0 893 905 1154 2127
Size: 893 12 249 973
Fragments arranged by size:
973 893 249 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 804 808 1248 1270 1557 1747 2127
Size: 804 4 440 22 287 190 380
Fragments arranged by size:
804 440 380 287 190 22 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1726 1758 2127
Size: 1726 32 369
Fragments arranged by size:
1726 369 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 222 1119 1524 2127
Size: 63 159 897 405 603
Fragments arranged by size:
897 603 405 159 63
XmnI GAAnn'nnTTC
Cuts at: 0 235 2127
Size: 235 1892
ZraI GAC'GTC
Cuts at: 0 257 2127
Size: 257 1870
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI
BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI
MfeI MscI MslI MspA1I NcoI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI
SmlI StuI StyI TaqII TstI XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI
BfrBI BglII BlpI BmtI BsaI BsaAI BsaBI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI