Restriction map for
pF1KSDA0881
(Linear) (Six-base) MAPSORT of: KIAA0881.seq Check: 1681 from: 1 to: 3147
KIAA0881.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 540 3147
Size: 540 2607
AatII G_ACGT'C
Cuts at: 0 623 3147
Size: 623 2524
AccI GT'mk_AC
Cuts at: 0 125 773 3147
Size: 125 648 2374
Fragments arranged by size:
2374 648 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 352 391 769 847 1202 1801 2795
Size: 49 303 39 378 78 355 599 994
Cuts at: 2795 3147
Size: 352
Fragments arranged by size:
994 599 378 355 352 303 78 49
39
AflII C'TTAA_G
Cuts at: 0 2353 3147
Size: 2353 794
AflIII A'CryG_T
Cuts at: 0 2269 3147
Size: 2269 878
AhdI GACnn_n'nnGTC
Cuts at: 0 624 3147
Size: 624 2523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1472 1966 2210 3147
Size: 1472 494 244 937
Fragments arranged by size:
1472 937 494 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 1672 1704 2458 2490 3147
Size: 217 32 1423 32 754 32 657
Fragments arranged by size:
1423 754 657 217 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 337 414 1518 1980 2445 2508 3015 3147
Size: 337 77 1104 462 465 63 507 132
Fragments arranged by size:
1104 507 465 462 337 132 77 63
ApaI G_GGCC'C
Cuts at: 0 2951 3061 3086 3147
Size: 2951 110 25 61
Fragments arranged by size:
2951 110 61 25
ApaLI G'TGCA_C
Cuts at: 0 352 1562 2030 3147
Size: 352 1210 468 1117
Fragments arranged by size:
1210 1117 468 352
ApoI r'AATT_y
Cuts at: 0 765 3147
Size: 765 2382
AvaI C'yCGr_G
Cuts at: 0 134 571 1042 1187 3030 3079 3147
Size: 134 437 471 145 1843 49 68
Fragments arranged by size:
1843 471 437 145 134 68 49
BanI G'GyrC_C
Cuts at: 0 528 550 567 957 973 1634 3075 3147
Size: 528 22 17 390 16 661 1441 72
Fragments arranged by size:
1441 661 528 390 72 22 17 16
BanII G_rGCy'C
Cuts at: 0 53 331 1010 1264 1793 2114 2951 2985
Size: 53 278 679 254 529 321 837 34
Cuts at: 2985 3061 3086 3147
Size: 76 25 61
Fragments arranged by size:
837 679 529 321 278 254 76 61
53 34 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 3147
Size: 775 2372
BbvCI CC'TCA_GC
Cuts at: 0 1537 3147
Size: 1537 1610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3087 3121 3147
Size: 3087 34 26
Fragments arranged by size:
3087 34 26
BglI GCCn_nnn'nGGC
Cuts at: 0 3018 3147
Size: 3018 129
BlpI GC'TnA_GC
Cuts at: 0 47 330 1474 2108 2336 2431 3147
Size: 47 283 1144 634 228 95 716
Fragments arranged by size:
1144 716 634 283 228 95 47
Bme1580I G_kGCm'C
Cuts at: 0 356 553 1064 1566 2034 2613 2853 2951
Size: 356 197 511 502 468 579 240 98
Cuts at: 2951 3061 3080 3086 3147
Size: 110 19 6 61
Fragments arranged by size:
579 511 502 468 356 240 197 110
98 61 19 6
BmgBI CAC'GTC
Cuts at: 0 2270 3147
Size: 2270 877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1470 3147
Size: 1470 1677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1068 1300 1546 1759 1777 1843 2068
Size: 718 350 232 246 213 18 66 225
Cuts at: 2068 2089 2173 2338 2530 2605 2638 2730 2876
Size: 21 84 165 192 75 33 92 146
Cuts at: 2876 2877 3147
Size: 1 270
Fragments arranged by size:
718 350 270 246 232 225 213 192
165 146 92 84 75 66 33 21
18 1
Bpu10I CC'TnA_GC
Cuts at: 0 1537 3088 3147
Size: 1537 1551 59
Fragments arranged by size:
1551 1537 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1603 2347 2707 3147
Size: 782 821 744 360 440
Fragments arranged by size:
821 782 744 440 360
BsaI GGTCTCn'nnnn_
Cuts at: 0 1382 2314 2452 3147
Size: 1382 932 138 695
Fragments arranged by size:
1382 932 695 138
BsaBI GATnn'nnATC
Cuts at: 0 2542 3147
Size: 2542 605
BsaHI Gr'CG_yC
Cuts at: 0 620 2901 3147
Size: 620 2281 246
Fragments arranged by size:
2281 620 246
BsaWI w'CCGG_w
Cuts at: 0 143 761 3147
Size: 143 618 2386
Fragments arranged by size:
2386 618 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 230 247 260 1672 1702 2458 2488
Size: 217 13 17 13 1412 30 756 30
Cuts at: 2488 3147
Size: 659
Fragments arranged by size:
1412 756 659 217 30 30 17 13
13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 1012 1153 1156 1159 1162 1165 1174
Size: 1003 9 141 3 3 3 3 9
Cuts at: 1174 1177 1180 1567 1693 1801 1891 2650 2861
Size: 3 3 387 126 108 90 759 211
Cuts at: 2861 3147
Size: 286
Fragments arranged by size:
1003 759 387 286 211 141 126 108
90 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 7 1063 1306 1332 1804 2139 2736 2814
Size: 7 1056 243 26 472 335 597 78
Cuts at: 2814 2943 3008 3147
Size: 129 65 139
Fragments arranged by size:
1056 597 472 335 243 139 129 78
65 26 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 2113 3147
Size: 409 1704 1034
Fragments arranged by size:
1704 1034 409
BsiHKAI G_wGCw'C
Cuts at: 0 53 296 356 734 845 1220 1559 1566
Size: 53 243 60 378 111 375 339 7
Cuts at: 1566 1793 2034 2114 2310 3147
Size: 227 241 80 196 837
Fragments arranged by size:
837 378 375 339 243 241 227 196
111 80 60 53 7
BsmI GAATG_Cn'
Cuts at: 0 690 3147
Size: 690 2457
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2260 2915 3147
Size: 2260 655 232
Fragments arranged by size:
2260 655 232
Bsp1286I G_dGCh'C
Cuts at: 0 53 296 331 356 553 734 845 1010
Size: 53 243 35 25 197 181 111 165
Cuts at: 1010 1064 1220 1264 1559 1566 1793 2034 2114
Size: 54 156 44 295 7 227 241 80
Cuts at: 2114 2310 2613 2853 2951 2985 3061 3080 3086
Size: 196 303 240 98 34 76 19 6
Cuts at: 3086 3147
Size: 61
Fragments arranged by size:
303 295 243 241 240 227 197 196
181 165 156 111 98 80 76 61
54 53 44 35 34 25 19 7
6
BspEI T'CCGG_A
Cuts at: 0 143 761 3147
Size: 143 618 2386
Fragments arranged by size:
2386 618 143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 649 926 1988 3147
Size: 540 109 277 1062 1159
Fragments arranged by size:
1159 1062 540 277 109
BsrBI CCG'CTC
Cuts at: 0 672 852 1478 1683 2691 3147
Size: 672 180 626 205 1008 456
Fragments arranged by size:
1008 672 626 456 205 180
BsrDI GCAATG_nn'
Cuts at: 0 707 3147
Size: 707 2440
BsrFI r'CCGG_y
Cuts at: 0 1250 3147
Size: 1250 1897
BssSI C'ACGA_G
Cuts at: 0 1661 2050 2563 3147
Size: 1661 389 513 584
Fragments arranged by size:
1661 584 513 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1666 2181 3147
Size: 1666 515 966
Fragments arranged by size:
1666 966 515
BstYI r'GATC_y
Cuts at: 0 941 1433 2276 2543 3147
Size: 941 492 843 267 604
Fragments arranged by size:
941 843 604 492 267
BstZ17I GTA'TAC
Cuts at: 0 126 3147
Size: 126 3021
Bsu36I CC'TnA_GG
Cuts at: 0 286 3147
Size: 286 2861
BtgI C'CryG_G
Cuts at: 0 105 396 587 2862 2973 3071 3147
Size: 105 291 191 2275 111 98 76
Fragments arranged by size:
2275 291 191 111 105 98 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 703 3147
Size: 703 2444
BtsI GCAGTG_nn'
Cuts at: 0 1870 1930 2032 2611 3045 3051 3147
Size: 1870 60 102 579 434 6 96
Fragments arranged by size:
1870 579 434 102 96 60 6
DraIII CAC_nnn'GTG
Cuts at: 0 1059 2962 2974 3147
Size: 1059 1903 12 173
Fragments arranged by size:
1903 1059 173 12
EaeI y'GGCC_r
Cuts at: 0 102 161 584 853 1196 3147
Size: 102 59 423 269 343 1951
Fragments arranged by size:
1951 423 343 269 102 59
EarI CTCTTCn'nnn_
Cuts at: 0 58 85 989 1130 1151 1359 2363 2627
Size: 58 27 904 141 21 208 1004 264
Cuts at: 2627 3147
Size: 520
Fragments arranged by size:
1004 904 520 264 208 141 58 27
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1888 2413 2993 3147
Size: 1888 525 580 154
Fragments arranged by size:
1888 580 525 154
EcoICRI GAG'CTC
Cuts at: 0 51 1791 2112 3147
Size: 51 1740 321 1035
Fragments arranged by size:
1740 1035 321 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 352 391 718 769 847 1068 1202
Size: 49 303 39 327 51 78 221 134
Cuts at: 1202 1300 1546 1759 1777 1801 1843 2068 2089
Size: 98 246 213 18 24 42 225 21
Cuts at: 2089 2173 2338 2530 2605 2638 2730 2795 2876
Size: 84 165 192 75 33 92 65 81
Cuts at: 2876 2877 3147
Size: 1 270
Fragments arranged by size:
327 303 270 246 225 221 213 192
165 134 98 92 84 81 78 75
65 51 49 42 39 33 24 21
18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 1738 1972 2876 3147
Size: 370 1368 234 904 271
Fragments arranged by size:
1368 904 370 271 234
EcoO109I rG'GnC_Cy
Cuts at: 0 33 411 981 1177 1977 1992 2481 2929
Size: 33 378 570 196 800 15 489 448
Cuts at: 2929 2947 2948 3058 3083 3147
Size: 18 1 110 25 64
Fragments arranged by size:
800 570 489 448 378 196 110 64
33 25 18 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 3147
Size: 852 2295
HaeII r_GCGC'y
Cuts at: 0 406 1082 1908 2446 3147
Size: 406 676 826 538 701
Fragments arranged by size:
826 701 676 538 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 2268 2300 3147
Size: 610 32 1626 32 847
Fragments arranged by size:
1626 847 610 32 32
HincII GTy'rAC
Cuts at: 0 774 1104 3147
Size: 774 330 2043
Fragments arranged by size:
2043 774 330
MscI TGG'CCA
Cuts at: 0 104 163 586 1198 3147
Size: 104 59 423 612 1949
Fragments arranged by size:
1949 612 423 104 59
MslI CAynn'nnrTG
Cuts at: 0 449 3147
Size: 449 2698
MspA1I CmG'CkG
Cuts at: 0 7 388 1473 1515 1575 1593 1779 2151
Size: 7 381 1085 42 60 18 186 372
Cuts at: 2151 2625 3073 3147
Size: 474 448 74
Fragments arranged by size:
1085 474 448 381 372 186 74 60
42 18 7
NcoI C'CATG_G
Cuts at: 0 105 587 2862 3147
Size: 105 482 2275 285
Fragments arranged by size:
2275 482 285 105
NspI r_CATG'y
Cuts at: 0 1017 1071 2197 2886 3147
Size: 1017 54 1126 689 261
Fragments arranged by size:
1126 1017 689 261 54
PasI CC'CwG_GG
Cuts at: 0 2787 3147
Size: 2787 360
PfoI T'CCnGG_A
Cuts at: 0 92 845 1436 1793 2261 3147
Size: 92 753 591 357 468 886
Fragments arranged by size:
886 753 591 468 357 92
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1672 1704 2458 2490 3147
Size: 217 32 1423 32 754 32 657
Fragments arranged by size:
1423 754 657 217 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 33 1977 1992 3147
Size: 33 1944 15 1155
Fragments arranged by size:
1944 1155 33 15
PshAI GACnn'nnGTC
Cuts at: 0 747 779 3147
Size: 747 32 2368
Fragments arranged by size:
2368 747 32
PspOMI G'GGCC_C
Cuts at: 0 2947 3057 3082 3147
Size: 2947 110 25 65
Fragments arranged by size:
2947 110 65 25
PstI C_TGCA'G
Cuts at: 0 923 1329 1574 1925 2126 2246 2654 3147
Size: 923 406 245 351 201 120 408 493
Fragments arranged by size:
923 493 408 406 351 245 201 120
PvuII CAG'CTG
Cuts at: 0 7 388 1473 1515 1779 2151 3147
Size: 7 381 1085 42 264 372 996
Fragments arranged by size:
1085 996 381 372 264 42 7
SacI G_AGCT'C
Cuts at: 0 53 1793 2114 3147
Size: 53 1740 321 1033
Fragments arranged by size:
1740 1033 321 53
SacII CC_GC'GG
Cuts at: 0 3074 3147
Size: 3074 73
SalI G'TCGA_C
Cuts at: 0 772 3147
Size: 772 2375
SapI GCTCTTCn'nnn_
Cuts at: 0 58 85 2363 3147
Size: 58 27 2278 784
Fragments arranged by size:
2278 784 58 27
ScaI AGT'ACT
Cuts at: 0 757 1912 3147
Size: 757 1155 1235
Fragments arranged by size:
1235 1155 757
SfcI C'TryA_G
Cuts at: 0 180 919 1325 1570 1921 2122 2242 2650
Size: 180 739 406 245 351 201 120 408
Cuts at: 2650 3147
Size: 497
Fragments arranged by size:
739 497 408 406 351 245 201 180
120
SmaI CCC'GGG
Cuts at: 0 136 1189 3081 3147
Size: 136 1053 1892 66
Fragments arranged by size:
1892 1053 136 66
SmlI C'TyrA_G
Cuts at: 0 797 1042 1582 2353 2362 2686 3147
Size: 797 245 540 771 9 324 461
Fragments arranged by size:
797 771 540 461 324 245 9
SphI G_CATG'C
Cuts at: 0 2197 2886 3147
Size: 2197 689 261
Fragments arranged by size:
2197 689 261
SrfI GCCC'GGGC
Cuts at: 0 3081 3147
Size: 3081 66
StuI AGG'CCT
Cuts at: 0 2713 3147
Size: 2713 434
StyI C'CwwG_G
Cuts at: 0 105 587 629 890 1704 2862 3147
Size: 105 482 42 261 814 1158 285
Fragments arranged by size:
1158 814 482 285 261 105 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 975 3147
Size: 873 102 2172
Fragments arranged by size:
2172 873 102
TatI w'GTAC_w
Cuts at: 0 755 1910 1934 2156 2291 3147
Size: 755 1155 24 222 135 856
Fragments arranged by size:
1155 856 755 222 135 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2011 2043 2958 2990 3127 3147
Size: 2011 32 915 32 137 20
Fragments arranged by size:
2011 915 137 32 32 20
XbaI T'CTAG_A
Cuts at: 0 345 3147
Size: 345 2802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 543 867 2064 2754 3121 3147
Size: 112 431 324 1197 690 367 26
Fragments arranged by size:
1197 690 431 367 324 112 26
XhoI C'TCGA_G
Cuts at: 0 1042 3147
Size: 1042 2105
XmaI C'CCGG_G
Cuts at: 0 134 1187 3079 3147
Size: 134 1053 1892 68
Fragments arranged by size:
1892 1053 134 68
XmnI GAAnn'nnTTC
Cuts at: 0 2257 3147
Size: 2257 890
ZraI GAC'GTC
Cuts at: 0 621 3147
Size: 621 2526
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NspI PasI PfoI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SalI
SapI ScaI SfcI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII
BmrI BmtI BsaAI BsiEI BsiWI BspHI BsrGI BssHII
BstBI BstEII BstXI ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PsiI PspXI PsrI PvuI RsrII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI Tth111I