Restriction map for
pF1KSDA0887
(Linear) (Six-base) MAPSORT of: KIAA0887.seq Check: 8072 from: 1 to: 1335
KIAA0887.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 733 1232 1335
Size: 608 125 499 103
Fragments arranged by size:
608 499 125 103
ApaLI G'TGCA_C
Cuts at: 0 581 1335
Size: 581 754
ApoI r'AATT_y
Cuts at: 0 1011 1335
Size: 1011 324
AvaI C'yCGr_G
Cuts at: 0 1115 1335
Size: 1115 220
BanI G'GyrC_C
Cuts at: 0 6 1335
Size: 6 1329
BbeI G_GCGC'C
Cuts at: 0 10 1335
Size: 10 1325
BbsI GAAGACnn'nnnn_
Cuts at: 0 450 1335
Size: 450 885
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 1335
Size: 646 689
BclI T'GATC_A
Cuts at: 0 540 1335
Size: 540 795
BglI GCCn_nnn'nGGC
Cuts at: 0 892 1335
Size: 892 443
Bme1580I G_kGCm'C
Cuts at: 0 450 585 1335
Size: 450 135 750
Fragments arranged by size:
750 450 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 1335
Size: 234 1101
BsaI GGTCTCn'nnnn_
Cuts at: 0 666 1335
Size: 666 669
BsaHI Gr'CG_yC
Cuts at: 0 7 1335
Size: 7 1328
BsaWI w'CCGG_w
Cuts at: 0 308 1335
Size: 308 1027
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 952 979 982 1009 1267 1335
Size: 28 924 27 3 27 258 68
Fragments arranged by size:
924 258 68 28 27 27 3
BsiHKAI G_wGCw'C
Cuts at: 0 585 1335
Size: 585 750
BsmI GAATG_Cn'
Cuts at: 0 1048 1335
Size: 1048 287
BsmBI CGTCTCn'nnnn_
Cuts at: 0 993 1119 1335
Size: 993 126 216
Fragments arranged by size:
993 216 126
Bsp1286I G_dGCh'C
Cuts at: 0 450 585 1335
Size: 450 135 750
Fragments arranged by size:
750 450 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 198 1335
Size: 198 1137
BsrBI CCG'CTC
Cuts at: 0 18 942 1335
Size: 18 924 393
Fragments arranged by size:
924 393 18
BsrGI T'GTAC_A
Cuts at: 0 138 1335
Size: 138 1197
BstYI r'GATC_y
Cuts at: 0 21 63 230 1335
Size: 21 42 167 1105
Fragments arranged by size:
1105 167 42 21
Bsu36I CC'TnA_GG
Cuts at: 0 11 647 1335
Size: 11 636 688
Fragments arranged by size:
688 636 11
DraIII CAC_nnn'GTG
Cuts at: 0 580 1335
Size: 580 755
EaeI y'GGCC_r
Cuts at: 0 698 1335
Size: 698 637
EarI CTCTTCn'nnn_
Cuts at: 0 356 425 625 1335
Size: 356 69 200 710
Fragments arranged by size:
710 356 200 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 972 1335
Size: 972 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 733 1232 1335
Size: 608 125 499 103
Fragments arranged by size:
608 499 125 103
EcoO109I rG'GnC_Cy
Cuts at: 0 1320 1335
Size: 1320 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1110 1335
Size: 1110 225
HaeII r_GCGC'y
Cuts at: 0 10 1335
Size: 10 1325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 644 676 1335
Size: 644 32 659
Fragments arranged by size:
659 644 32
KasI G'GCGC_C
Cuts at: 0 6 1335
Size: 6 1329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1335
Size: 1019 316
MscI TGG'CCA
Cuts at: 0 700 1335
Size: 700 635
MslI CAynn'nnrTG
Cuts at: 0 536 613 706 1335
Size: 536 77 93 629
Fragments arranged by size:
629 536 93 77
MspA1I CmG'CkG
Cuts at: 0 220 1335
Size: 220 1115
NarI GG'CG_CC
Cuts at: 0 7 1335
Size: 7 1328
NspI r_CATG'y
Cuts at: 0 632 1335
Size: 632 703
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1335
Size: 105 1230
PpuMI rG'GwC_Cy
Cuts at: 0 1320 1335
Size: 1320 15
PstI C_TGCA'G
Cuts at: 0 56 209 1335
Size: 56 153 1126
Fragments arranged by size:
1126 153 56
PvuII CAG'CTG
Cuts at: 0 220 1335
Size: 220 1115
SapI GCTCTTCn'nnn_
Cuts at: 0 356 625 1335
Size: 356 269 710
Fragments arranged by size:
710 356 269
SbfI CC_TGCA'GG
Cuts at: 0 209 1335
Size: 209 1126
SexAI A'CCwGG_T
Cuts at: 0 815 1335
Size: 815 520
SfcI C'TryA_G
Cuts at: 0 52 205 234 1276 1335
Size: 52 153 29 1042 59
Fragments arranged by size:
1042 153 59 52 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 892 1335
Size: 892 443
SfoI GGC'GCC
Cuts at: 0 8 1335
Size: 8 1327
SmlI C'TyrA_G
Cuts at: 0 249 1115 1335
Size: 249 866 220
Fragments arranged by size:
866 249 220
SphI G_CATG'C
Cuts at: 0 632 1335
Size: 632 703
StuI AGG'CCT
Cuts at: 0 752 886 1335
Size: 752 134 449
Fragments arranged by size:
752 449 134
StyI C'CwwG_G
Cuts at: 0 104 262 1335
Size: 104 158 1073
Fragments arranged by size:
1073 158 104
TatI w'GTAC_w
Cuts at: 0 138 1335
Size: 138 1197
XhoI C'TCGA_G
Cuts at: 0 1115 1335
Size: 1115 220
XmnI GAAnn'nnTTC
Cuts at: 0 1199 1335
Size: 1199 136
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BanI BbeI BbsI BciVI
BclI BglI Bme1580I BpuEI BsaI BsaHI BsaWI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrGI BstYI
Bsu36I DraIII EaeI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NspI PflMI PpuMI PstI PvuII SapI SbfI SexAI
SfcI SfiI SfoI SmlI SphI StuI StyI TatI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaXI BseYI BsgI BsiEI BsiWI
BspEI BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XmaI ZraI