Restriction map for
pF1KA0890
(Linear) (Six-base) MAPSORT of: KIAA0890.seq Check: 2532 from: 1 to: 3582
KIAA0890.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 224 832 3178 3582
Size: 224 608 2346 404
Fragments arranged by size:
2346 608 404 224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 2296 2715 3433 3582
Size: 955 1341 419 718 149
Fragments arranged by size:
1341 955 718 419 149
AfeI AGC'GCT
Cuts at: 0 1420 1792 3582
Size: 1420 372 1790
Fragments arranged by size:
1790 1420 372
AhdI GACnn_n'nnGTC
Cuts at: 0 621 3582
Size: 621 2961
AleI CACnn'nnGTG
Cuts at: 0 84 3326 3582
Size: 84 3242 256
Fragments arranged by size:
3242 256 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2003 3181 3530 3582
Size: 2003 1178 349 52
Fragments arranged by size:
2003 1178 349 52
AlwNI CAG_nnn'CTG
Cuts at: 0 898 1248 1905 2619 3582
Size: 898 350 657 714 963
Fragments arranged by size:
963 898 714 657 350
ApaI G_GGCC'C
Cuts at: 0 36 1525 1593 3432 3582
Size: 36 1489 68 1839 150
Fragments arranged by size:
1839 1489 150 68 36
ApaLI G'TGCA_C
Cuts at: 0 229 2110 3280 3319 3582
Size: 229 1881 1170 39 263
Fragments arranged by size:
1881 1170 263 229 39
AvaI C'yCGr_G
Cuts at: 0 119 1051 1580 2720 2870 3582
Size: 119 932 529 1140 150 712
Fragments arranged by size:
1140 932 712 529 150 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1794 1827 3381 3414 3582
Size: 1794 33 1554 33 168
Fragments arranged by size:
1794 1554 168 33 33
BamHI G'GATC_C
Cuts at: 0 2009 3582
Size: 2009 1573
BanI G'GyrC_C
Cuts at: 0 814 881 1047 1441 1830 1913 1919 3338
Size: 814 67 166 394 389 83 6 1419
Cuts at: 3338 3582
Size: 244
Fragments arranged by size:
1419 814 394 389 244 166 83 67
6
BanII G_rGCy'C
Cuts at: 0 36 192 714 1058 1525 1593 3432 3582
Size: 36 156 522 344 467 68 1839 150
Fragments arranged by size:
1839 522 467 344 156 150 68 36
BbeI G_GCGC'C
Cuts at: 0 885 3342 3582
Size: 885 2457 240
Fragments arranged by size:
2457 885 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 1393 2284 2494 3249 3582
Size: 1393 891 210 755 333
Fragments arranged by size:
1393 891 755 333 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 334 3240 3274 3454 3488 3582
Size: 300 34 2906 34 180 34 94
Fragments arranged by size:
2906 300 180 94 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1624 3582
Size: 1624 1958
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1281 1652 2031 2348 2363 3582
Size: 709 572 371 379 317 15 1219
Fragments arranged by size:
1219 709 572 379 371 317 15
BlpI GC'TnA_GC
Cuts at: 0 326 3582
Size: 326 3256
Bme1580I G_kGCm'C
Cuts at: 0 36 233 817 1052 1211 1446 1525 1593
Size: 36 197 584 235 159 235 79 68
Cuts at: 1593 2114 3284 3323 3432 3582
Size: 521 1170 39 109 150
Fragments arranged by size:
1170 584 521 235 235 197 159 150
109 79 68 39 36
BmgBI CAC'GTC
Cuts at: 0 1665 1695 3318 3582
Size: 1665 30 1623 264
Fragments arranged by size:
1665 1623 264 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 714 1528 1843 3003 3582
Size: 714 814 315 1160 579
Fragments arranged by size:
1160 814 714 579 315
BmtI G_CTAG'C
Cuts at: 0 427 3292 3582
Size: 427 2865 290
Fragments arranged by size:
2865 427 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1812 3408 3582
Size: 1812 1596 174
Fragments arranged by size:
1812 1596 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1669 2047 2144 2317 2419 2548 2905
Size: 1435 234 378 97 173 102 129 357
Cuts at: 2905 3403 3582
Size: 498 179
Fragments arranged by size:
1435 498 378 357 234 179 173 129
102 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 1708 2467 3582
Size: 648 1060 759 1115
Fragments arranged by size:
1115 1060 759 648
BsaI GGTCTCn'nnnn_
Cuts at: 0 1012 1069 3582
Size: 1012 57 2513
Fragments arranged by size:
2513 1012 57
BsaAI yAC'GTr
Cuts at: 0 228 3582
Size: 228 3354
BsaHI Gr'CG_yC
Cuts at: 0 462 882 3339 3582
Size: 462 420 2457 243
Fragments arranged by size:
2457 462 420 243
BsaWI w'CCGG_w
Cuts at: 0 465 3582
Size: 465 3117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2290 2320 2491 2521 2851 2881 3582
Size: 2290 30 171 30 330 30 701
Fragments arranged by size:
2290 701 330 171 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 592 1240 2854 3043 3046 3049 3261
Size: 583 9 648 1614 189 3 3 212
Cuts at: 3261 3511 3582
Size: 250 71
Fragments arranged by size:
1614 648 583 250 212 189 71 9
3 3
BseYI C'CCAG_C
Cuts at: 0 35 632 1011 1022 1295 1403 1642 2834
Size: 35 597 379 11 273 108 239 1192
Cuts at: 2834 2908 3484 3582
Size: 74 576 98
Fragments arranged by size:
1192 597 576 379 273 239 108 98
74 35 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1587 1891 1963 2071 2236 2671 2976
Size: 250 1337 304 72 108 165 435 305
Cuts at: 2976 3154 3458 3582
Size: 178 304 124
Fragments arranged by size:
1337 435 305 304 304 250 178 165
124 108 72
BsiHKAI G_wGCw'C
Cuts at: 0 233 1766 1862 2114 3284 3323 3503 3582
Size: 233 1533 96 252 1170 39 180 79
Fragments arranged by size:
1533 1170 252 233 180 96 79 39
BsmI GAATG_Cn'
Cuts at: 0 1945 3582
Size: 1945 1637
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1240 3582
Size: 1240 2342
Bsp1286I G_dGCh'C
Cuts at: 0 36 192 233 714 817 1052 1058 1211
Size: 36 156 41 481 103 235 6 153
Cuts at: 1211 1446 1525 1593 1766 1862 2114 3284 3323
Size: 235 79 68 173 96 252 1170 39
Cuts at: 3323 3432 3503 3582
Size: 109 71 79
Fragments arranged by size:
1170 481 252 235 235 173 156 153
109 103 96 79 79 71 68 41
39 36 6
BspEI T'CCGG_A
Cuts at: 0 465 3582
Size: 465 3117
BspHI T'CATG_A
Cuts at: 0 1766 3582
Size: 1766 1816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1913 2903 3380 3582
Size: 1913 990 477 202
Fragments arranged by size:
1913 990 477 202
BsrBI CCG'CTC
Cuts at: 0 330 548 1689 3450 3582
Size: 330 218 1141 1761 132
Fragments arranged by size:
1761 1141 330 218 132
BsrDI GCAATG_nn'
Cuts at: 0 1458 2195 3582
Size: 1458 737 1387
Fragments arranged by size:
1458 1387 737
BsrFI r'CCGG_y
Cuts at: 0 1275 1910 1916 2393 2830 3061 3582
Size: 1275 635 6 477 437 231 521
Fragments arranged by size:
1275 635 521 477 437 231 6
BssSI C'ACGA_G
Cuts at: 0 2083 3582
Size: 2083 1499
BstEII G'GTnAC_C
Cuts at: 0 3102 3582
Size: 3102 480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 2517 2664 3582
Size: 288 2229 147 918
Fragments arranged by size:
2229 918 288 147
BstYI r'GATC_y
Cuts at: 0 2009 2429 3582
Size: 2009 420 1153
Fragments arranged by size:
2009 1153 420
BtgI C'CryG_G
Cuts at: 0 2925 3582
Size: 2925 657
BtsI GCAGTG_nn'
Cuts at: 0 262 2546 2805 3582
Size: 262 2284 259 777
Fragments arranged by size:
2284 777 262 259
ClaI AT'CG_AT
Cuts at: 0 320 1985 3582
Size: 320 1665 1597
Fragments arranged by size:
1665 1597 320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2178 2213 3582
Size: 2178 35 1369
Fragments arranged by size:
2178 1369 35
DraIII CAC_nnn'GTG
Cuts at: 0 1353 3582
Size: 1353 2229
DrdI GACnn_nn'nnGTC
Cuts at: 0 220 828 2286 3582
Size: 220 608 1458 1296
Fragments arranged by size:
1458 1296 608 220
EaeI y'GGCC_r
Cuts at: 0 746 1880 2186 2648 3059 3582
Size: 746 1134 306 462 411 523
Fragments arranged by size:
1134 746 523 462 411 306
EarI CTCTTCn'nnn_
Cuts at: 0 180 560 578 931 1603 3582
Size: 180 380 18 353 672 1979
Fragments arranged by size:
1979 672 380 353 180 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2994 3582
Size: 2994 588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1435 1669 2047 2144 2296 2317 2419
Size: 955 480 234 378 97 152 21 102
Cuts at: 2419 2548 2715 2905 3403 3433 3582
Size: 129 167 190 498 30 149
Fragments arranged by size:
955 498 480 378 234 190 167 152
149 129 102 97 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 101 1190 1723 2065 2509 3082 3582
Size: 101 1089 533 342 444 573 500
Fragments arranged by size:
1089 573 533 500 444 342 101
EcoO109I rG'GnC_Cy
Cuts at: 0 132 382 529 537 1171 1290 1522 1589
Size: 132 250 147 8 634 119 232 67
Cuts at: 1589 1590 1728 2070 2724 3429 3541 3582
Size: 1 138 342 654 705 112 41
Fragments arranged by size:
705 654 634 342 250 232 147 138
132 119 112 67 41 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2199 3582
Size: 2199 1383
FseI GG_CCGG'CC
Cuts at: 0 3065 3582
Size: 3065 517
FspI TGC'GCA
Cuts at: 0 1944 2173 2437 3460 3565 3582
Size: 1944 229 264 1023 105 17
Fragments arranged by size:
1944 1023 264 229 105 17
FspAI rTGC'GCAy
Cuts at: 0 1944 3582
Size: 1944 1638
HaeII r_GCGC'y
Cuts at: 0 885 1029 1422 1794 2061 2337 2619 3342
Size: 885 144 393 372 267 276 282 723
Cuts at: 3342 3582
Size: 240
Fragments arranged by size:
885 723 393 372 282 276 267 240
144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 102 130 134 607 639 1262 1294
Size: 98 4 28 4 473 32 623 32
Cuts at: 1294 2491 2523 2851 2883 3582
Size: 1197 32 328 32 699
Fragments arranged by size:
1197 699 623 473 328 98 32 32
32 32 28 4 4
HincII GTy'rAC
Cuts at: 0 93 3179 3582
Size: 93 3086 403
Fragments arranged by size:
3086 403 93
KasI G'GCGC_C
Cuts at: 0 881 3338 3582
Size: 881 2457 244
Fragments arranged by size:
2457 881 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1541 2141 3582
Size: 1196 345 600 1441
Fragments arranged by size:
1441 1196 600 345
MscI TGG'CCA
Cuts at: 0 748 1882 2188 2650 3582
Size: 748 1134 306 462 932
Fragments arranged by size:
1134 932 748 462 306
MslI CAynn'nnrTG
Cuts at: 0 84 236 1045 1828 1985 2125 3085 3326
Size: 84 152 809 783 157 140 960 241
Cuts at: 3326 3582
Size: 256
Fragments arranged by size:
960 809 783 256 241 157 152 140
84
MspA1I CmG'CkG
Cuts at: 0 343 357 455 501 1407 1448 1503 1737
Size: 343 14 98 46 906 41 55 234
Cuts at: 1737 2354 2589 2795 3510 3574 3582
Size: 617 235 206 715 64 8
Fragments arranged by size:
906 715 617 343 235 234 206 98
64 55 46 41 14 8
NaeI GCC'GGC
Cuts at: 0 2395 2832 3063 3582
Size: 2395 437 231 519
Fragments arranged by size:
2395 519 437 231
NarI GG'CG_CC
Cuts at: 0 882 3339 3582
Size: 882 2457 243
Fragments arranged by size:
2457 882 243
NgoMIV G'CCGG_C
Cuts at: 0 2393 2830 3061 3582
Size: 2393 437 231 521
Fragments arranged by size:
2393 521 437 231
NheI G'CTAG_C
Cuts at: 0 423 3288 3582
Size: 423 2865 294
Fragments arranged by size:
2865 423 294
NspI r_CATG'y
Cuts at: 0 208 2082 2236 2481 3582
Size: 208 1874 154 245 1101
Fragments arranged by size:
1874 1101 245 208 154
PasI CC'CwG_GG
Cuts at: 0 2073 2607 3432 3582
Size: 2073 534 825 150
Fragments arranged by size:
2073 825 534 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 810 1518 3582
Size: 810 708 2064
Fragments arranged by size:
2064 810 708
PfoI T'CCnGG_A
Cuts at: 0 2063 2564 3263 3582
Size: 2063 501 699 319
Fragments arranged by size:
2063 699 501 319
PpuMI rG'GwC_Cy
Cuts at: 0 132 382 529 2724 3541 3582
Size: 132 250 147 2195 817 41
Fragments arranged by size:
2195 817 250 147 132 41
PspOMI G'GGCC_C
Cuts at: 0 32 1521 1589 3428 3582
Size: 32 1489 68 1839 154
Fragments arranged by size:
1839 1489 154 68 32
PstI C_TGCA'G
Cuts at: 0 342 348 1136 1634 1736 3582
Size: 342 6 788 498 102 1846
Fragments arranged by size:
1846 788 498 342 102 6
PvuII CAG'CTG
Cuts at: 0 343 357 455 501 1407 3510 3574 3582
Size: 343 14 98 46 906 2103 64 8
Fragments arranged by size:
2103 906 343 98 64 46 14 8
SalI G'TCGA_C
Cuts at: 0 3177 3582
Size: 3177 405
SanDI GG'GwC_CC
Cuts at: 0 2724 3582
Size: 2724 858
SapI GCTCTTCn'nnn_
Cuts at: 0 180 578 931 1603 3582
Size: 180 398 353 672 1979
Fragments arranged by size:
1979 672 398 353 180
SexAI A'CCwGG_T
Cuts at: 0 2001 2453 3582
Size: 2001 452 1129
Fragments arranged by size:
2001 1129 452
SfcI C'TryA_G
Cuts at: 0 338 344 1132 1630 1732 2743 3582
Size: 338 6 788 498 102 1011 839
Fragments arranged by size:
1011 839 788 498 338 102 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2348 3582
Size: 2348 1234
SfoI GGC'GCC
Cuts at: 0 883 3340 3582
Size: 883 2457 242
Fragments arranged by size:
2457 883 242
SmaI CCC'GGG
Cuts at: 0 2722 2872 3582
Size: 2722 150 710
Fragments arranged by size:
2722 710 150
SmlI C'TyrA_G
Cuts at: 0 119 663 1723 2446 3582
Size: 119 544 1060 723 1136
Fragments arranged by size:
1136 1060 723 544 119
SpeI A'CTAG_T
Cuts at: 0 219 3582
Size: 219 3363
SphI G_CATG'C
Cuts at: 0 2082 3582
Size: 2082 1500
StuI AGG'CCT
Cuts at: 0 2965 3582
Size: 2965 617
StyI C'CwwG_G
Cuts at: 0 749 917 1154 2282 2510 2651 3582
Size: 749 168 237 1128 228 141 931
Fragments arranged by size:
1128 931 749 237 228 168 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 96 258 1003 1444 1480 2018 2705 2922
Size: 96 162 745 441 36 538 687 217
Cuts at: 2922 3322 3582
Size: 400 260
Fragments arranged by size:
745 687 538 441 400 260 217 162
96 36
TatI w'GTAC_w
Cuts at: 0 3020 3582
Size: 3020 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2290 2322 3582
Size: 2290 32 1260
Fragments arranged by size:
2290 1260 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 990 3582
Size: 741 249 2592
Fragments arranged by size:
2592 741 249
XhoI C'TCGA_G
Cuts at: 0 119 3582
Size: 119 3463
XmaI C'CCGG_G
Cuts at: 0 2720 2870 3582
Size: 2720 150 712
Fragments arranged by size:
2720 712 150
Enzymes that do cut:
AccI AcuI AfeI AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI
BstYI BtgI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SalI SanDI SapI
SexAI SfcI SfiI SfoI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AloI
ApoI AscI AseI AsiSI AvrII BbvCI BclI BfrBI
BglII Bpu10I BsaBI BsiEI BsiWI BsrGI BssHII BstAPI
BstBI BstZ17I Bsu36I BtgZI DraI EagI EcoICRI EcoRI
EcoRV HindIII HpaI KpnI MfeI MluI NcoI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SbfI ScaI SgfI SgrAI SnaBI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI