Restriction map for
pF1KSDA0891
(Linear) (Six-base) MAPSORT of: KIAA0891.seq Check: 9019 from: 1 to: 3954
KIAA0891.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 1029 1522 3954
Size: 460 569 493 2432
Fragments arranged by size:
2432 569 493 460
AccI GT'mk_AC
Cuts at: 0 3587 3954
Size: 3587 367
Acc65I G'GTAC_C
Cuts at: 0 2921 3954
Size: 2921 1033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2434 3954
Size: 2434 1520
AfeI AGC'GCT
Cuts at: 0 1135 2419 3082 3325 3954
Size: 1135 1284 663 243 629
Fragments arranged by size:
1284 1135 663 629 243
AflIII A'CryG_T
Cuts at: 0 899 3607 3917 3954
Size: 899 2708 310 37
Fragments arranged by size:
2708 899 310 37
AhdI GACnn_n'nnGTC
Cuts at: 0 2250 3954
Size: 2250 1704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 568 2689 3954
Size: 568 2121 1265
Fragments arranged by size:
2121 1265 568
AlwNI CAG_nnn'CTG
Cuts at: 0 805 1542 2797 3142 3750 3954
Size: 805 737 1255 345 608 204
Fragments arranged by size:
1255 805 737 608 345 204
ApaI G_GGCC'C
Cuts at: 0 41 880 1019 2858 3791 3809 3826 3954
Size: 41 839 139 1839 933 18 17 128
Fragments arranged by size:
1839 933 839 139 128 41 18 17
ApaLI G'TGCA_C
Cuts at: 0 895 1074 3506 3954
Size: 895 179 2432 448
Fragments arranged by size:
2432 895 448 179
ApoI r'AATT_y
Cuts at: 0 675 2828 3954
Size: 675 2153 1126
Fragments arranged by size:
2153 1126 675
AvaI C'yCGr_G
Cuts at: 0 712 826 1005 1265 1553 2066 2780 3158
Size: 712 114 179 260 288 513 714 378
Cuts at: 3158 3223 3954
Size: 65 731
Fragments arranged by size:
731 714 712 513 378 288 260 179
114 65
AvrII C'CTAG_G
Cuts at: 0 115 3696 3954
Size: 115 3581 258
Fragments arranged by size:
3581 258 115
BamHI G'GATC_C
Cuts at: 0 3132 3954
Size: 3132 822
BanI G'GyrC_C
Cuts at: 0 576 1401 1678 2019 2346 2921 3219 3290
Size: 576 825 277 341 327 575 298 71
Cuts at: 3290 3771 3883 3954
Size: 481 112 71
Fragments arranged by size:
825 576 575 481 341 327 298 277
112 71 71
BanII G_rGCy'C
Cuts at: 0 41 667 827 880 1019 2075 2659 2738
Size: 41 626 160 53 139 1056 584 79
Cuts at: 2738 2858 3791 3809 3826 3954
Size: 120 933 18 17 128
Fragments arranged by size:
1056 933 626 584 160 139 128 120
79 53 41 18 17
BbeI G_GCGC'C
Cuts at: 0 3294 3954
Size: 3294 660
BbsI GAAGACnn'nnnn_
Cuts at: 0 314 486 3954
Size: 314 172 3468
Fragments arranged by size:
3468 314 172
BbvCI CC'TCA_GC
Cuts at: 0 189 1880 2759 3128 3710 3758 3954
Size: 189 1691 879 369 582 48 196
Fragments arranged by size:
1691 879 582 369 196 189 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 98 3436 3470 3954
Size: 64 34 3338 34 484
Fragments arranged by size:
3338 484 64 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3945 3954
Size: 3945 9
BfrBI ATG'CAT
Cuts at: 0 1397 3954
Size: 1397 2557
BglI GCCn_nnn'nGGC
Cuts at: 0 662 688 1720 3151 3727 3954
Size: 662 26 1032 1431 576 227
Fragments arranged by size:
1431 1032 662 576 227 26
BglII A'GATC_T
Cuts at: 0 914 3954
Size: 914 3040
Bme1580I G_kGCm'C
Cuts at: 0 41 880 899 1019 1078 1681 1969 2024
Size: 41 839 19 120 59 603 288 55
Cuts at: 2024 2351 2816 2858 3510 3791 3809 3826 3886
Size: 327 465 42 652 281 18 17 60
Cuts at: 3886 3954
Size: 68
Fragments arranged by size:
839 652 603 465 327 288 281 120
68 60 59 55 42 41 19 18
17
BmgBI CAC'GTC
Cuts at: 0 3546 3954
Size: 3546 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 718 1417 2955 3825 3954
Size: 718 699 1538 870 129
Fragments arranged by size:
1538 870 718 699 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2886 3954
Size: 2886 1068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 323 439 716 1168 2713 2722 3052
Size: 70 253 116 277 452 1545 9 330
Cuts at: 3052 3772 3954
Size: 720 182
Fragments arranged by size:
1545 720 452 330 277 253 182 116
70 9
Bpu10I CC'TnA_GC
Cuts at: 0 189 1048 1880 2303 2732 2759 3128 3400
Size: 189 859 832 423 429 27 369 272
Cuts at: 3400 3710 3758 3954
Size: 310 48 196
Fragments arranged by size:
859 832 429 423 369 310 272 196
189 48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 915 3304 3954
Size: 260 655 2389 650
Fragments arranged by size:
2389 655 650 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 1834 1989 2708 3954
Size: 137 1697 155 719 1246
Fragments arranged by size:
1697 1246 719 155 137
BsaAI yAC'GTr
Cuts at: 0 590 900 906 3835 3954
Size: 590 310 6 2929 119
Fragments arranged by size:
2929 590 310 119 6
BsaBI GATnn'nnATC
Cuts at: 0 110 3954
Size: 110 3844
BsaHI Gr'CG_yC
Cuts at: 0 1632 3291 3954
Size: 1632 1659 663
Fragments arranged by size:
1659 1632 663
BsaWI w'CCGG_w
Cuts at: 0 743 3866 3954
Size: 743 3123 88
Fragments arranged by size:
3123 743 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 321 351 381 1424 1454 2720 2750 3082
Size: 321 30 30 1043 30 1266 30 332
Cuts at: 3082 3112 3220 3250 3954
Size: 30 108 30 704
Fragments arranged by size:
1266 1043 704 332 321 108 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 263 295 685 796 1273 1459 1462 2260
Size: 263 32 390 111 477 186 3 798
Cuts at: 2260 3241 3442 3775 3778 3954
Size: 981 201 333 3 176
Fragments arranged by size:
981 798 477 390 333 263 201 186
176 111 32 3 3
BseYI C'CCAG_C
Cuts at: 0 205 1136 2350 2698 2815 3036 3439 3954
Size: 205 931 1214 348 117 221 403 515
Fragments arranged by size:
1214 931 515 403 348 221 205 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 538 985 1145 1783 2393 2649 3334
Size: 389 149 447 160 638 610 256 685
Cuts at: 3334 3954
Size: 620
Fragments arranged by size:
685 638 620 610 447 389 256 160
149
BsiHKAI G_wGCw'C
Cuts at: 0 899 1078 1236 2738 3510 3686 3914 3954
Size: 899 179 158 1502 772 176 228 40
Fragments arranged by size:
1502 899 772 228 179 176 158 40
BsmI GAATG_Cn'
Cuts at: 0 1817 2952 3954
Size: 1817 1135 1002
Fragments arranged by size:
1817 1135 1002
Bsp1286I G_dGCh'C
Cuts at: 0 41 667 827 880 899 1019 1078 1236
Size: 41 626 160 53 19 120 59 158
Cuts at: 1236 1681 1969 2024 2075 2351 2659 2738 2816
Size: 445 288 55 51 276 308 79 78
Cuts at: 2816 2858 3510 3686 3791 3809 3826 3886 3914
Size: 42 652 176 105 18 17 60 28
Cuts at: 3914 3954
Size: 40
Fragments arranged by size:
652 626 445 308 288 276 176 160
158 120 105 79 78 60 59 55
53 51 42 41 40 28 19 18
17
BspEI T'CCGG_A
Cuts at: 0 743 3954
Size: 743 3211
BspHI T'CATG_A
Cuts at: 0 315 1520 3954
Size: 315 1205 2434
Fragments arranged by size:
2434 1205 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 460 661 1029 1522 2189 2547 3661 3954
Size: 460 201 368 493 667 358 1114 293
Fragments arranged by size:
1114 667 493 460 368 358 293 201
BsrBI CCG'CTC
Cuts at: 0 1583 2310 3954
Size: 1583 727 1644
Fragments arranged by size:
1644 1583 727
BsrFI r'CCGG_y
Cuts at: 0 2016 3145 3163 3635 3768 3954
Size: 2016 1129 18 472 133 186
Fragments arranged by size:
2016 1129 472 186 133 18
BssSI C'ACGA_G
Cuts at: 0 1163 1801 3907 3954
Size: 1163 638 2106 47
Fragments arranged by size:
2106 1163 638 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 802 805 1183 1514 3282 3410 3954
Size: 802 3 378 331 1768 128 544
Fragments arranged by size:
1768 802 544 378 331 128 3
BstEII G'GTnAC_C
Cuts at: 0 2745 3954
Size: 2745 1209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 3117 3954
Size: 205 2912 837
Fragments arranged by size:
2912 837 205
BstYI r'GATC_y
Cuts at: 0 111 914 3132 3954
Size: 111 803 2218 822
Fragments arranged by size:
2218 822 803 111
Bsu36I CC'TnA_GG
Cuts at: 0 171 994 3209 3776 3954
Size: 171 823 2215 567 178
Fragments arranged by size:
2215 823 567 178 171
BtgI C'CryG_G
Cuts at: 0 737 1340 1844 3887 3954
Size: 737 603 504 2043 67
Fragments arranged by size:
2043 737 603 504 67
BtsI GCAGTG_nn'
Cuts at: 0 487 562 710 797 1078 2123 2671 3497
Size: 487 75 148 87 281 1045 548 826
Cuts at: 3497 3546 3954
Size: 49 408
Fragments arranged by size:
1045 826 548 487 408 281 148 87
75 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 797 832 3954
Size: 797 35 3122
Fragments arranged by size:
3122 797 35
EaeI y'GGCC_r
Cuts at: 0 1637 2846 3165 3489 3954
Size: 1637 1209 319 324 465
Fragments arranged by size:
1637 1209 465 324 319
EarI CTCTTCn'nnn_
Cuts at: 0 368 1448 1454 3202 3631 3954
Size: 368 1080 6 1748 429 323
Fragments arranged by size:
1748 1080 429 368 323 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2206 3084 3654 3954
Size: 2206 878 570 300
Fragments arranged by size:
2206 878 570 300
EcoICRI GAG'CTC
Cuts at: 0 2736 3954
Size: 2736 1218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 323 439 716 1168 2434 2713 2722
Size: 70 253 116 277 452 1266 279 9
Cuts at: 2722 3052 3772 3954
Size: 330 720 182
Fragments arranged by size:
1266 720 452 330 279 277 253 182
116 70 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 607 689 1936 3954
Size: 607 82 1247 2018
Fragments arranged by size:
2018 1247 607 82
EcoO109I rG'GnC_Cy
Cuts at: 0 37 38 406 876 1016 1143 1152 1806
Size: 37 1 368 470 140 127 9 654
Cuts at: 1806 2490 2806 3659 3700 3788 3796 3805 3806
Size: 684 316 853 41 88 8 9 1
Cuts at: 3806 3823 3954
Size: 17 131
Fragments arranged by size:
853 684 654 470 368 316 140 131
127 88 41 37 17 9 9 8
1 1
FseI GG_CCGG'CC
Cuts at: 0 3167 3954
Size: 3167 787
HaeII r_GCGC'y
Cuts at: 0 1137 1669 2340 2421 3084 3294 3327 3558
Size: 1137 532 671 81 663 210 33 231
Cuts at: 3558 3954
Size: 396
Fragments arranged by size:
1137 671 663 532 396 231 210 81
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 117 147 149 712 744 814 846
Size: 115 2 30 2 563 32 70 32
Cuts at: 846 1113 1145 2134 2166 2236 2268 2272 2304
Size: 267 32 989 32 70 32 4 32
Cuts at: 2304 2878 2910 3124 3156 3954
Size: 574 32 214 32 798
Fragments arranged by size:
989 798 574 563 267 214 115 70
70 32 32 32 32 32 32 32
32 30 4 2 2
HindIII A'AGCT_T
Cuts at: 0 388 3954
Size: 388 3566
KasI G'GCGC_C
Cuts at: 0 3290 3954
Size: 3290 664
KpnI G_GTAC'C
Cuts at: 0 2925 3954
Size: 2925 1029
MluI A'CGCG_T
Cuts at: 0 3607 3954
Size: 3607 347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1571 2378 3014 3954
Size: 1225 346 807 636 940
Fragments arranged by size:
1225 940 807 636 346
MscI TGG'CCA
Cuts at: 0 1639 2848 3491 3954
Size: 1639 1209 643 463
Fragments arranged by size:
1639 1209 643 463
MslI CAynn'nnrTG
Cuts at: 0 1339 2933 3954
Size: 1339 1594 1021
Fragments arranged by size:
1594 1339 1021
MspA1I CmG'CkG
Cuts at: 0 205 417 639 1896 2385 2742 2881 3003
Size: 205 212 222 1257 489 357 139 122
Cuts at: 3003 3279 3435 3709 3954
Size: 276 156 274 245
Fragments arranged by size:
1257 489 357 276 274 245 222 212
205 156 139 122
NaeI GCC'GGC
Cuts at: 0 3165 3637 3954
Size: 3165 472 317
Fragments arranged by size:
3165 472 317
NarI GG'CG_CC
Cuts at: 0 3291 3954
Size: 3291 663
NcoI C'CATG_G
Cuts at: 0 737 1340 3954
Size: 737 603 2614
Fragments arranged by size:
2614 737 603
NgoMIV G'CCGG_C
Cuts at: 0 3163 3635 3954
Size: 3163 472 319
Fragments arranged by size:
3163 472 319
NsiI A_TGCA'T
Cuts at: 0 1399 3954
Size: 1399 2555
NspI r_CATG'y
Cuts at: 0 1351 1397 3954
Size: 1351 46 2557
Fragments arranged by size:
2557 1351 46
PasI CC'CwG_GG
Cuts at: 0 757 3665 3792 3800 3954
Size: 757 2908 127 8 154
Fragments arranged by size:
2908 757 154 127 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 1429 2241 3954
Size: 1429 812 1713
Fragments arranged by size:
1713 1429 812
PfoI T'CCnGG_A
Cuts at: 0 1562 3733 3954
Size: 1562 2171 221
Fragments arranged by size:
2171 1562 221
PmlI CAC'GTG
Cuts at: 0 590 900 3835 3954
Size: 590 310 2935 119
Fragments arranged by size:
2935 590 310 119
PpuMI rG'GwC_Cy
Cuts at: 0 406 1806 2806 3796 3954
Size: 406 1400 1000 990 158
Fragments arranged by size:
1400 1000 990 406 158
PshAI GACnn'nnGTC
Cuts at: 0 2804 3954
Size: 2804 1150
PspOMI G'GGCC_C
Cuts at: 0 37 876 1015 2854 3787 3805 3822 3954
Size: 37 839 139 1839 933 18 17 132
Fragments arranged by size:
1839 933 839 139 132 37 18 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1816 1848 2500 2532 3954
Size: 1816 32 652 32 1422
Fragments arranged by size:
1816 1422 652 32 32
PstI C_TGCA'G
Cuts at: 0 416 922 3708 3954
Size: 416 506 2786 246
Fragments arranged by size:
2786 506 416 246
PvuII CAG'CTG
Cuts at: 0 205 417 2742 2881 3279 3435 3709 3954
Size: 205 212 2325 139 398 156 274 245
Fragments arranged by size:
2325 398 274 245 212 205 156 139
SacI G_AGCT'C
Cuts at: 0 2738 3954
Size: 2738 1216
SanDI GG'GwC_CC
Cuts at: 0 3796 3954
Size: 3796 158
ScaI AGT'ACT
Cuts at: 0 938 3954
Size: 938 3016
SfcI C'TryA_G
Cuts at: 0 412 918 3048 3704 3954
Size: 412 506 2130 656 250
Fragments arranged by size:
2130 656 506 412 250
SfoI GGC'GCC
Cuts at: 0 3292 3954
Size: 3292 662
SgrAI Cr'CCGG_yG
Cuts at: 0 3635 3954
Size: 3635 319
SmaI CCC'GGG
Cuts at: 0 714 1007 1267 2782 3160 3954
Size: 714 293 260 1515 378 794
Fragments arranged by size:
1515 794 714 378 293 260
SmlI C'TyrA_G
Cuts at: 0 275 930 3319 3954
Size: 275 655 2389 635
Fragments arranged by size:
2389 655 635 275
SpeI A'CTAG_T
Cuts at: 0 61 3954
Size: 61 3893
SrfI GCCC'GGGC
Cuts at: 0 1267 3160 3954
Size: 1267 1893 794
Fragments arranged by size:
1893 1267 794
StuI AGG'CCT
Cuts at: 0 3232 3268 3954
Size: 3232 36 686
Fragments arranged by size:
3232 686 36
StyI C'CwwG_G
Cuts at: 0 28 115 737 776 812 1289 1340 1979
Size: 28 87 622 39 36 477 51 639
Cuts at: 1979 2642 3110 3696 3954
Size: 663 468 586 258
Fragments arranged by size:
663 639 622 586 477 468 258 87
51 39 36 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1159 1353 1696 3028 3704 3807 3954
Size: 1159 194 343 1332 676 103 147
Fragments arranged by size:
1332 1159 676 343 194 147 103
TatI w'GTAC_w
Cuts at: 0 936 1946 3621 3954
Size: 936 1010 1675 333
Fragments arranged by size:
1675 1010 936 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 351 383 1424 1456 2720 2752 2760 2792
Size: 351 32 1041 32 1264 32 8 32
Cuts at: 2792 3954
Size: 1162
Fragments arranged by size:
1264 1162 1041 351 32 32 32 32
8
Tth111I GACn'n_nGTC
Cuts at: 0 129 724 3954
Size: 129 595 3230
Fragments arranged by size:
3230 595 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 979 2094 2688 3954
Size: 783 196 1115 594 1266
Fragments arranged by size:
1266 1115 783 594 196
XmaI C'CCGG_G
Cuts at: 0 712 1005 1265 2780 3158 3954
Size: 712 293 260 1515 378 796
Fragments arranged by size:
1515 796 712 378 293 260
XmnI GAAnn'nnTTC
Cuts at: 0 2832 3954
Size: 2832 1122
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI HaeII Hin4I HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PsrI PstI PvuII SacI SanDI ScaI SfcI
SfoI SgrAI SmaI SmlI SpeI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AleI AloI AscI AseI
AsiSI BaeI BclI BlpI BmtI BsiEI BsiWI BsmBI
BsrDI BsrGI BssHII BstBI BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FspI FspAI
HincII HpaI MfeI NdeI NheI NotI NruI PacI
PciI PmeI PpiI PsiI PspXI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI SgfI SnaBI SphI
SspI SwaI XbaI XhoI ZraI