Restriction map for
pF1KA0892
(Linear) (Six-base) MAPSORT of: KIAA0892.seq Check: 4451 from: 1 to: 1839
KIAA0892.orf
With 182 enzymes: SgfI *
June 11, 2014 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 212 723 1839
Size: 212 511 1116
Fragments arranged by size:
1116 511 212
AccI GT'mk_AC
Cuts at: 0 875 1839
Size: 875 964
Acc65I G'GTAC_C
Cuts at: 0 74 1839
Size: 74 1765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1477 1839
Size: 778 699 362
Fragments arranged by size:
778 699 362
AflIII A'CryG_T
Cuts at: 0 860 1601 1839
Size: 860 741 238
Fragments arranged by size:
860 741 238
AhdI GACnn_n'nnGTC
Cuts at: 0 483 1775 1839
Size: 483 1292 64
Fragments arranged by size:
1292 483 64
AleI CACnn'nnGTG
Cuts at: 0 863 1839
Size: 863 976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 1839
Size: 592 1247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 1839
Size: 1186 32 621
Fragments arranged by size:
1186 621 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 1713 1839
Size: 1239 474 126
Fragments arranged by size:
1239 474 126
ApaLI G'TGCA_C
Cuts at: 0 142 610 1839
Size: 142 468 1229
Fragments arranged by size:
1229 468 142
ApoI r'AATT_y
Cuts at: 0 316 360 1839
Size: 316 44 1479
Fragments arranged by size:
1479 316 44
AvaI C'yCGr_G
Cuts at: 0 524 1839
Size: 524 1315
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1600 1633 1839
Size: 1600 33 206
Fragments arranged by size:
1600 206 33
BanI G'GyrC_C
Cuts at: 0 74 1566 1839
Size: 74 1492 273
Fragments arranged by size:
1492 273 74
BanII G_rGCy'C
Cuts at: 0 217 1400 1839
Size: 217 1183 439
Fragments arranged by size:
1183 439 217
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 1839
Size: 681 1158
BbvCI CC'TCA_GC
Cuts at: 0 565 1472 1839
Size: 565 907 367
Fragments arranged by size:
907 565 367
BglI GCCn_nnn'nGGC
Cuts at: 0 31 61 1839
Size: 31 30 1778
Fragments arranged by size:
1778 31 30
BglII A'GATC_T
Cuts at: 0 404 1076 1839
Size: 404 672 763
Fragments arranged by size:
763 672 404
BlpI GC'TnA_GC
Cuts at: 0 98 1839
Size: 98 1741
Bme1580I G_kGCm'C
Cuts at: 0 146 614 1839
Size: 146 468 1225
Fragments arranged by size:
1225 468 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 1194 1839
Size: 1194 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 688 937 979 1039 1342 1702 1839
Size: 286 402 249 42 60 303 360 137
Fragments arranged by size:
402 360 303 286 249 137 60 42
Bpu10I CC'TnA_GC
Cuts at: 0 8 565 1472 1839
Size: 8 557 907 367
Fragments arranged by size:
907 557 367 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 952 1839
Size: 490 462 887
Fragments arranged by size:
887 490 462
BsaI GGTCTCn'nnnn_
Cuts at: 0 1631 1839
Size: 1631 208
BsaAI yAC'GTr
Cuts at: 0 1766 1839
Size: 1766 73
BsaBI GATnn'nnATC
Cuts at: 0 403 1839
Size: 403 1436
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 847 877 1839
Size: 259 30 558 30 962
Fragments arranged by size:
962 558 259 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 553 1839
Size: 553 1286
BseYI C'CCAG_C
Cuts at: 0 210 817 1152 1533 1839
Size: 210 607 335 381 306
Fragments arranged by size:
607 381 335 306 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 781 1126 1810 1839
Size: 445 336 345 684 29
Fragments arranged by size:
684 445 345 336 29
BsiEI CG_ry'CG
Cuts at: 0 65 1839
Size: 65 1774
BsiHKAI G_wGCw'C
Cuts at: 0 104 146 614 704 860 1025 1839
Size: 104 42 468 90 156 165 814
Fragments arranged by size:
814 468 165 156 104 90 42
Bsp1286I G_dGCh'C
Cuts at: 0 104 146 217 614 704 860 1025 1400
Size: 104 42 71 397 90 156 165 375
Cuts at: 1400 1839
Size: 439
Fragments arranged by size:
439 397 375 165 156 104 90 71
42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 723 1839
Size: 212 511 1116
Fragments arranged by size:
1116 511 212
BsrDI GCAATG_nn'
Cuts at: 0 1147 1839
Size: 1147 692
BsrFI r'CCGG_y
Cuts at: 0 907 1484 1839
Size: 907 577 355
Fragments arranged by size:
907 577 355
BssHII G'CGCG_C
Cuts at: 0 253 1839
Size: 253 1586
BssSI C'ACGA_G
Cuts at: 0 69 1504 1839
Size: 69 1435 335
Fragments arranged by size:
1435 335 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 609 618 900 1149 1713 1839
Size: 150 459 9 282 249 564 126
Fragments arranged by size:
564 459 282 249 150 126 9
BstBI TT'CG_AA
Cuts at: 0 305 1839
Size: 305 1534
BstEII G'GTnAC_C
Cuts at: 0 708 1839
Size: 708 1131
BstYI r'GATC_y
Cuts at: 0 404 535 809 1076 1592 1839
Size: 404 131 274 267 516 247
Fragments arranged by size:
516 404 274 267 247 131
BtgI C'CryG_G
Cuts at: 0 1061 1208 1664 1839
Size: 1061 147 456 175
Fragments arranged by size:
1061 456 175 147
BtsI GCAGTG_nn'
Cuts at: 0 144 429 778 793 1362 1627 1839
Size: 144 285 349 15 569 265 212
Fragments arranged by size:
569 349 285 265 212 144 15
EaeI y'GGCC_r
Cuts at: 0 62 1826 1839
Size: 62 1764 13
Fragments arranged by size:
1764 62 13
EagI C'GGCC_G
Cuts at: 0 62 1839
Size: 62 1777
EarI CTCTTCn'nnn_
Cuts at: 0 448 733 841 1123 1405 1839
Size: 448 285 108 282 282 434
Fragments arranged by size:
448 434 285 282 282 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1839
Size: 121 1718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 688 778 937 979 1039 1342 1477
Size: 286 402 90 159 42 60 303 135
Cuts at: 1477 1702 1839
Size: 225 137
Fragments arranged by size:
402 303 286 225 159 137 135 90
60 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 268 568 919 1839
Size: 268 300 351 920
Fragments arranged by size:
920 351 300 268
EcoO109I rG'GnC_Cy
Cuts at: 0 477 945 1476 1839
Size: 477 468 531 363
Fragments arranged by size:
531 477 468 363
EcoRI G'AATT_C
Cuts at: 0 360 1839
Size: 360 1479
EcoRV GAT'ATC
Cuts at: 0 284 1839
Size: 284 1555
FspI TGC'GCA
Cuts at: 0 50 1839
Size: 50 1789
HaeII r_GCGC'y
Cuts at: 0 85 556 1069 1839
Size: 85 471 513 770
Fragments arranged by size:
770 513 471 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1761 1793 1839
Size: 469 32 1260 32 46
Fragments arranged by size:
1260 469 46 32 32
HincII GTy'rAC
Cuts at: 0 1176 1839
Size: 1176 663
KpnI G_GTAC'C
Cuts at: 0 78 1839
Size: 78 1761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1839
Size: 1148 691
MscI TGG'CCA
Cuts at: 0 1828 1839
Size: 1828 11
MslI CAynn'nnrTG
Cuts at: 0 803 863 865 1030 1564 1839
Size: 803 60 2 165 534 275
Fragments arranged by size:
803 534 275 165 60 2
MspA1I CmG'CkG
Cuts at: 0 22 210 384 618 765 829 957 1095
Size: 22 188 174 234 147 64 128 138
Cuts at: 1095 1140 1614 1713 1839
Size: 45 474 99 126
Fragments arranged by size:
474 234 188 174 147 138 128 126
99 64 45 22
NaeI GCC'GGC
Cuts at: 0 909 1839
Size: 909 930
NcoI C'CATG_G
Cuts at: 0 1664 1839
Size: 1664 175
NgoMIV G'CCGG_C
Cuts at: 0 907 1839
Size: 907 932
NspI r_CATG'y
Cuts at: 0 864 1605 1839
Size: 864 741 234
Fragments arranged by size:
864 741 234
PciI A'CATG_T
Cuts at: 0 860 1601 1839
Size: 860 741 238
Fragments arranged by size:
860 741 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 720 1839
Size: 720 1119
PfoI T'CCnGG_A
Cuts at: 0 1595 1718 1839
Size: 1595 123 121
Fragments arranged by size:
1595 123 121
PmlI CAC'GTG
Cuts at: 0 1766 1839
Size: 1766 73
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 1839
Size: 1742 32 65
Fragments arranged by size:
1742 65 32
PpuMI rG'GwC_Cy
Cuts at: 0 477 1476 1839
Size: 477 999 363
Fragments arranged by size:
999 477 363
PstI C_TGCA'G
Cuts at: 0 155 209 605 770 985 1073 1741 1839
Size: 155 54 396 165 215 88 668 98
Fragments arranged by size:
668 396 215 165 155 98 88 54
PvuII CAG'CTG
Cuts at: 0 210 765 957 1095 1140 1614 1713 1839
Size: 210 555 192 138 45 474 99 126
Fragments arranged by size:
555 474 210 192 138 126 99 45
SapI GCTCTTCn'nnn_
Cuts at: 0 448 733 1123 1405 1839
Size: 448 285 390 282 434
Fragments arranged by size:
448 434 390 285 282
SbfI CC_TGCA'GG
Cuts at: 0 155 1839
Size: 155 1684
SexAI A'CCwGG_T
Cuts at: 0 479 878 1839
Size: 479 399 961
Fragments arranged by size:
961 479 399
SfcI C'TryA_G
Cuts at: 0 151 205 601 766 981 1069 1311 1737
Size: 151 54 396 165 215 88 242 426
Cuts at: 1737 1839
Size: 102
Fragments arranged by size:
426 396 242 215 165 151 102 88
54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 61 1839
Size: 61 1778
SmaI CCC'GGG
Cuts at: 0 526 1839
Size: 526 1313
SmlI C'TyrA_G
Cuts at: 0 469 967 1839
Size: 469 498 872
Fragments arranged by size:
872 498 469
StuI AGG'CCT
Cuts at: 0 1735 1839
Size: 1735 104
StyI C'CwwG_G
Cuts at: 0 851 1664 1839
Size: 851 813 175
Fragments arranged by size:
851 813 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 727 1839
Size: 493 234 1112
Fragments arranged by size:
1112 493 234
TatI w'GTAC_w
Cuts at: 0 347 932 1160 1839
Size: 347 585 228 679
Fragments arranged by size:
679 585 347 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 278 291 310 847 879 1839
Size: 259 19 13 19 537 32 960
Fragments arranged by size:
960 537 259 32 19 19 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1804 1821 1839
Size: 1804 17 18
Fragments arranged by size:
1804 18 17
XmaI C'CCGG_G
Cuts at: 0 524 1839
Size: 524 1315
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PfoI
PmlI PpiI PpuMI PstI PvuII SapI SbfI SexAI
SfcI SfiI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI ApaI AscI AseI
AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI
BmgBI BmtI BplI BsaHI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI FalI FseI
FspAI HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI