Restriction map for
pF1KA0896
(Linear) (Six-base) MAPSORT of: KIAA0896.seq Check: 4092 from: 1 to: 8394
KIAA0896.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1456 8394
Size: 1456 6938
AatII G_ACGT'C
Cuts at: 0 7 304 8394
Size: 7 297 8090
Fragments arranged by size:
8090 297 7
AccI GT'mk_AC
Cuts at: 0 2217 2664 3006 4106 5426 6102 7400 7968
Size: 2217 447 342 1100 1320 676 1298 568
Cuts at: 7968 8394
Size: 426
Fragments arranged by size:
2217 1320 1298 1100 676 568 447 426
342
Acc65I G'GTAC_C
Cuts at: 0 1591 2449 3484 5266 8394
Size: 1591 858 1035 1782 3128
Fragments arranged by size:
3128 1782 1591 1035 858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 836 1796 2299 3947 5006 5567 6155
Size: 610 226 960 503 1648 1059 561 588
Cuts at: 6155 7157 8394
Size: 1002 1237
Fragments arranged by size:
1648 1237 1059 1002 960 610 588 561
503 226
AflIII A'CryG_T
Cuts at: 0 1846 7653 8394
Size: 1846 5807 741
Fragments arranged by size:
5807 1846 741
AgeI A'CCGG_T
Cuts at: 0 3163 8394
Size: 3163 5231
AhdI GACnn_n'nnGTC
Cuts at: 0 8314 8394
Size: 8314 80
AleI CACnn'nnGTG
Cuts at: 0 782 2744 8394
Size: 782 1962 5650
Fragments arranged by size:
5650 1962 782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6194 7715 8313 8394
Size: 6194 1521 598 81
Fragments arranged by size:
6194 1521 598 81
AlwNI CAG_nnn'CTG
Cuts at: 0 349 547 2095 2721 4858 4939 5299 5446
Size: 349 198 1548 626 2137 81 360 147
Cuts at: 5446 6046 6622 7135 7768 8394
Size: 600 576 513 633 626
Fragments arranged by size:
2137 1548 633 626 626 600 576 513
360 349 198 147 81
ApoI r'AATT_y
Cuts at: 0 1108 2041 5628 6670 7989 8379 8394
Size: 1108 933 3587 1042 1319 390 15
Fragments arranged by size:
3587 1319 1108 1042 933 390 15
AvaI C'yCGr_G
Cuts at: 0 285 452 473 881 8394
Size: 285 167 21 408 7513
Fragments arranged by size:
7513 408 285 167 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1583 1616 4199 4232 8394
Size: 1583 33 2583 33 4162
Fragments arranged by size:
4162 2583 1583 33 33
BamHI G'GATC_C
Cuts at: 0 424 2493 3042 3099 8394
Size: 424 2069 549 57 5295
Fragments arranged by size:
5295 2069 549 424 57
BanI G'GyrC_C
Cuts at: 0 37 1591 2023 2449 3484 5266 6566 6961
Size: 37 1554 432 426 1035 1782 1300 395
Cuts at: 6961 8394
Size: 1433
Fragments arranged by size:
1782 1554 1433 1300 1035 432 426 395
37
BanII G_rGCy'C
Cuts at: 0 453 1128 8394
Size: 453 675 7266
Fragments arranged by size:
7266 675 453
BbeI G_GCGC'C
Cuts at: 0 6570 8394
Size: 6570 1824
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 2287 4826 7101 8394
Size: 824 1463 2539 2275 1293
Fragments arranged by size:
2539 2275 1463 1293 824
BbvCI CC'TCA_GC
Cuts at: 0 4787 8394
Size: 4787 3607
BciVI GTATCCnnnnn_n'
Cuts at: 0 5895 6952 7390 8394
Size: 5895 1057 438 1004
Fragments arranged by size:
5895 1057 1004 438
BclI T'GATC_A
Cuts at: 0 4575 6153 8066 8394
Size: 4575 1578 1913 328
Fragments arranged by size:
4575 1913 1578 328
BfrBI ATG'CAT
Cuts at: 0 1858 8394
Size: 1858 6536
BglII A'GATC_T
Cuts at: 0 1729 6744 8178 8394
Size: 1729 5015 1434 216
Fragments arranged by size:
5015 1729 1434 216
BlpI GC'TnA_GC
Cuts at: 0 1454 1781 5710 7277 8394
Size: 1454 327 3929 1567 1117
Fragments arranged by size:
3929 1567 1454 1117 327
Bme1580I G_kGCm'C
Cuts at: 0 40 1440 8394
Size: 40 1400 6954
Fragments arranged by size:
6954 1400 40
BmgBI CAC'GTC
Cuts at: 0 5698 8394
Size: 5698 2696
BmrI ACTGGGnnnn_n'
Cuts at: 0 5956 8394
Size: 5956 2438
BmtI G_CTAG'C
Cuts at: 0 1766 5315 5672 8394
Size: 1766 3549 357 2722
Fragments arranged by size:
3549 2722 1766 357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 927 3838 6766 8394
Size: 927 2911 2928 1628
Fragments arranged by size:
2928 2911 1628 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 422 467 752 1652 1820 2040 3038
Size: 414 8 45 285 900 168 220 998
Cuts at: 3038 3277 3572 3625 4575 4757 8394
Size: 239 295 53 950 182 3637
Fragments arranged by size:
3637 998 950 900 414 295 285 239
220 182 168 53 45 8
Bpu10I CC'TnA_GC
Cuts at: 0 4787 7160 8394
Size: 4787 2373 1234
Fragments arranged by size:
4787 2373 1234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1626 2704 3420 4995 8394
Size: 205 1421 1078 716 1575 3399
Fragments arranged by size:
3399 1575 1421 1078 716 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 1958 5053 5783 7001 8394
Size: 1958 3095 730 1218 1393
Fragments arranged by size:
3095 1958 1393 1218 730
BsaAI yAC'GTr
Cuts at: 0 952 1847 8394
Size: 952 895 6547
Fragments arranged by size:
6547 952 895
BsaBI GATnn'nnATC
Cuts at: 0 2753 6737 6743 8394
Size: 2753 3984 6 1651
Fragments arranged by size:
3984 2753 1651 6
BsaHI Gr'CG_yC
Cuts at: 0 4 301 6567 8394
Size: 4 297 6266 1827
Fragments arranged by size:
6266 1827 297 4
BsaWI w'CCGG_w
Cuts at: 0 3163 8394
Size: 3163 5231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3598 3628 7032 7035 7062 7065 7823 7853
Size: 3598 30 3404 3 27 3 758 30
Cuts at: 7853 8394
Size: 541
Fragments arranged by size:
3598 3404 758 541 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 5756 7013 7053 7056 7395 8394
Size: 574 5182 1257 40 3 339 999
Fragments arranged by size:
5182 1257 999 574 339 40 3
BseYI C'CCAG_C
Cuts at: 0 527 1657 3974 5162 7272 8394
Size: 527 1130 2317 1188 2110 1122
Fragments arranged by size:
2317 2110 1188 1130 1122 527
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1898 4466 5720 6419 8394
Size: 1898 2568 1254 699 1975
Fragments arranged by size:
2568 1975 1898 1254 699
BsiHKAI G_wGCw'C
Cuts at: 0 453 1379 3563 3821 8394
Size: 453 926 2184 258 4573
Fragments arranged by size:
4573 2184 926 453 258
BsmI GAATG_Cn'
Cuts at: 0 2544 3255 4014 5793 8394
Size: 2544 711 759 1779 2601
Fragments arranged by size:
2601 2544 1779 759 711
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2970 7757 8394
Size: 2970 4787 637
Fragments arranged by size:
4787 2970 637
Bsp1286I G_dGCh'C
Cuts at: 0 40 453 1128 1379 1440 3563 3821 8394
Size: 40 413 675 251 61 2123 258 4573
Fragments arranged by size:
4573 2123 675 413 258 251 61 40
BspHI T'CATG_A
Cuts at: 0 3654 3948 4752 6423 8394
Size: 3654 294 804 1671 1971
Fragments arranged by size:
3654 1971 1671 804 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1456 2139 7051 8394
Size: 1456 683 4912 1343
Fragments arranged by size:
4912 1456 1343 683
BsrBI CCG'CTC
Cuts at: 0 1413 8394
Size: 1413 6981
BsrDI GCAATG_nn'
Cuts at: 0 1428 2363 2882 4152 4396 5885 6893 7226
Size: 1428 935 519 1270 244 1489 1008 333
Cuts at: 7226 7793 7961 8394
Size: 567 168 433
Fragments arranged by size:
1489 1428 1270 1008 935 567 519 433
333 244 168
BsrFI r'CCGG_y
Cuts at: 0 464 3163 6347 8394
Size: 464 2699 3184 2047
Fragments arranged by size:
3184 2699 2047 464
BsrGI T'GTAC_A
Cuts at: 0 4171 6819 7212 8394
Size: 4171 2648 393 1182
Fragments arranged by size:
4171 2648 1182 393
BssSI C'ACGA_G
Cuts at: 0 1235 5738 7208 8394
Size: 1235 4503 1470 1186
Fragments arranged by size:
4503 1470 1235 1186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4939 5299 8394
Size: 4939 360 3095
Fragments arranged by size:
4939 3095 360
BstEII G'GTnAC_C
Cuts at: 0 835 8394
Size: 835 7559
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5113 6147 8394
Size: 5113 1034 2247
Fragments arranged by size:
5113 2247 1034
BstYI r'GATC_y
Cuts at: 0 424 738 1729 2493 2508 3042 3099 3142
Size: 424 314 991 764 15 534 57 43
Cuts at: 3142 6192 6744 7386 8178 8394
Size: 3050 552 642 792 216
Fragments arranged by size:
3050 991 792 764 642 552 534 424
314 216 57 43 15
BstZ17I GTA'TAC
Cuts at: 0 2218 4107 5427 6103 8394
Size: 2218 1889 1320 676 2291
Fragments arranged by size:
2291 2218 1889 1320 676
Bsu36I CC'TnA_GG
Cuts at: 0 381 8394
Size: 381 8013
BtgI C'CryG_G
Cuts at: 0 331 7334 7437 8394
Size: 331 7003 103 957
Fragments arranged by size:
7003 957 331 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3109 7166 8394
Size: 3109 4057 1228
Fragments arranged by size:
4057 3109 1228
BtsI GCAGTG_nn'
Cuts at: 0 1087 1379 1881 1954 2249 2405 3776 4533
Size: 1087 292 502 73 295 156 1371 757
Cuts at: 4533 4928 4971 5149 6870 8209 8394
Size: 395 43 178 1721 1339 185
Fragments arranged by size:
1721 1371 1339 1087 757 502 395 295
292 185 178 156 73 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2145 2180 6149 6184 8394
Size: 2145 35 3969 35 2210
Fragments arranged by size:
3969 2210 2145 35 35
DraI TTT'AAA
Cuts at: 0 101 8394
Size: 101 8293
EaeI y'GGCC_r
Cuts at: 0 2465 3366 6048 6201 8394
Size: 2465 901 2682 153 2193
Fragments arranged by size:
2682 2465 2193 901 153
EarI CTCTTCn'nnn_
Cuts at: 0 1919 1937 1961 2816 4091 4703 4736 5618
Size: 1919 18 24 855 1275 612 33 882
Cuts at: 5618 5634 6038 8394
Size: 16 404 2356
Fragments arranged by size:
2356 1919 1275 882 855 612 404 33
24 18 16
EcoICRI GAG'CTC
Cuts at: 0 451 8394
Size: 451 7943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 422 467 610 752 836 1652 1796
Size: 414 8 45 143 142 84 816 144
Cuts at: 1796 1820 2040 2299 3038 3277 3572 3625 3947
Size: 24 220 259 739 239 295 53 322
Cuts at: 3947 4575 4757 5006 5567 6155 7157 8394
Size: 628 182 249 561 588 1002 1237
Fragments arranged by size:
1237 1002 816 739 628 588 561 414
322 295 259 249 239 220 182 144
143 142 84 53 45 24 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 432 8394
Size: 432 7962
EcoO109I rG'GnC_Cy
Cuts at: 0 1822 3202 7118 8394
Size: 1822 1380 3916 1276
Fragments arranged by size:
3916 1822 1380 1276
EcoRI G'AATT_C
Cuts at: 0 5628 8394
Size: 5628 2766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1183 4075 5518 6023 7687 8394
Size: 1183 2892 1443 505 1664 707
Fragments arranged by size:
2892 1664 1443 1183 707 505
HaeII r_GCGC'y
Cuts at: 0 5717 6570 7437 8394
Size: 5717 853 867 957
Fragments arranged by size:
5717 957 867 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 621 653 730 762 961 993
Size: 487 32 102 32 77 32 199 32
Cuts at: 993 1599 1631 1846 1878 1936 1968 2291 2323
Size: 606 32 215 32 58 32 323 32
Cuts at: 2323 4458 4490 4984 5016 5809 5841 6013 6045
Size: 2135 32 494 32 793 32 172 32
Cuts at: 6045 6818 6850 7188 7220 7950 7982 8300 8332
Size: 773 32 338 32 730 32 318 32
Cuts at: 8332 8394
Size: 62
Fragments arranged by size:
2135 793 773 730 606 494 487 338
323 318 215 199 172 102 77 62
58 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1256 1907 2665 8394
Size: 1256 651 758 5729
Fragments arranged by size:
5729 1256 758 651
HindIII A'AGCT_T
Cuts at: 0 1740 2209 5500 8113 8394
Size: 1740 469 3291 2613 281
Fragments arranged by size:
3291 2613 1740 469 281
HpaI GTT'AAC
Cuts at: 0 1256 8394
Size: 1256 7138
KasI G'GCGC_C
Cuts at: 0 6566 8394
Size: 6566 1828
KpnI G_GTAC'C
Cuts at: 0 1595 2453 3488 5270 8394
Size: 1595 858 1035 1782 3124
Fragments arranged by size:
3124 1782 1595 1035 858
MfeI C'AATT_G
Cuts at: 0 1865 4367 7686 7805 8394
Size: 1865 2502 3319 119 589
Fragments arranged by size:
3319 2502 1865 589 119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2818 4501 6093 6319 6591 7241 8394
Size: 2818 1683 1592 226 272 650 1153
Fragments arranged by size:
2818 1683 1592 1153 650 272 226
MscI TGG'CCA
Cuts at: 0 2467 6050 8394
Size: 2467 3583 2344
Fragments arranged by size:
3583 2467 2344
MslI CAynn'nnrTG
Cuts at: 0 782 1433 1845 2744 2774 3466 4754 5111
Size: 782 651 412 899 30 692 1288 357
Cuts at: 5111 5786 5798 5978 7252 8394
Size: 675 12 180 1274 1142
Fragments arranged by size:
1288 1274 1142 899 782 692 675 651
412 357 180 30 12
MspA1I CmG'CkG
Cuts at: 0 30 392 445 1458 1712 2995 3136 3358
Size: 30 362 53 1013 254 1283 141 222
Cuts at: 3358 4858 5224 5290 5299 5446 7281 8394
Size: 1500 366 66 9 147 1835 1113
Fragments arranged by size:
1835 1500 1283 1113 1013 366 362 254
222 147 141 66 53 30 9
NaeI GCC'GGC
Cuts at: 0 466 8394
Size: 466 7928
NarI GG'CG_CC
Cuts at: 0 6567 8394
Size: 6567 1827
NcoI C'CATG_G
Cuts at: 0 331 7334 7437 8394
Size: 331 7003 103 957
Fragments arranged by size:
7003 957 331 103
NdeI CA'TA_TG
Cuts at: 0 5844 8394
Size: 5844 2550
NgoMIV G'CCGG_C
Cuts at: 0 464 8394
Size: 464 7930
NheI G'CTAG_C
Cuts at: 0 1762 5311 5668 8394
Size: 1762 3549 357 2726
Fragments arranged by size:
3549 2726 1762 357
NsiI A_TGCA'T
Cuts at: 0 1860 8394
Size: 1860 6534
NspI r_CATG'y
Cuts at: 0 2773 5839 6342 7257 7657 7954 8394
Size: 2773 3066 503 915 400 297 440
Fragments arranged by size:
3066 2773 915 503 440 400 297
PciI A'CATG_T
Cuts at: 0 7653 8394
Size: 7653 741
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 7549 8394
Size: 18 7531 845
Fragments arranged by size:
7531 845 18
PfoI T'CCnGG_A
Cuts at: 0 2046 5592 7382 8394
Size: 2046 3546 1790 1012
Fragments arranged by size:
3546 2046 1790 1012
PmlI CAC'GTG
Cuts at: 0 1847 8394
Size: 1847 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 8394
Size: 268 32 8094
Fragments arranged by size:
8094 268 32
PpuMI rG'GwC_Cy
Cuts at: 0 1822 3202 8394
Size: 1822 1380 5192
Fragments arranged by size:
5192 1822 1380
PshAI GACnn'nnGTC
Cuts at: 0 1902 8394
Size: 1902 6492
PspXI vC'TCGA_Gb
Cuts at: 0 473 8394
Size: 473 7921
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7931 7963 8394
Size: 7931 32 431
Fragments arranged by size:
7931 431 32
PstI C_TGCA'G
Cuts at: 0 2117 2726 4935 5289 5295 6042 8123 8394
Size: 2117 609 2209 354 6 747 2081 271
Fragments arranged by size:
2209 2117 2081 747 609 354 271 6
PvuII CAG'CTG
Cuts at: 0 445 1458 1712 2995 3358 4858 5290 5299
Size: 445 1013 254 1283 363 1500 432 9
Cuts at: 5299 5446 7281 8394
Size: 147 1835 1113
Fragments arranged by size:
1835 1500 1283 1113 1013 445 432 363
254 147 9
SacI G_AGCT'C
Cuts at: 0 453 8394
Size: 453 7941
SalI G'TCGA_C
Cuts at: 0 2663 8394
Size: 2663 5731
SapI GCTCTTCn'nnn_
Cuts at: 0 1937 4091 8394
Size: 1937 2154 4303
Fragments arranged by size:
4303 2154 1937
SbfI CC_TGCA'GG
Cuts at: 0 2117 8394
Size: 2117 6277
ScaI AGT'ACT
Cuts at: 0 4503 8394
Size: 4503 3891
SfcI C'TryA_G
Cuts at: 0 1304 1310 1713 2113 2321 2609 2713 2722
Size: 1304 6 403 400 208 288 104 9
Cuts at: 2722 3221 3997 4931 5285 5291 6038 8119 8394
Size: 499 776 934 354 6 747 2081 275
Fragments arranged by size:
2081 1304 934 776 747 499 403 400
354 288 275 208 104 9 6 6
SfoI GGC'GCC
Cuts at: 0 6568 8394
Size: 6568 1826
SmlI C'TyrA_G
Cuts at: 0 184 452 473 881 1605 2683 3435 5010
Size: 184 268 21 408 724 1078 752 1575
Cuts at: 5010 8394
Size: 3384
Fragments arranged by size:
3384 1575 1078 752 724 408 268 184
21
SpeI A'CTAG_T
Cuts at: 0 4212 8394
Size: 4212 4182
SphI G_CATG'C
Cuts at: 0 2773 8394
Size: 2773 5621
SspI AAT'ATT
Cuts at: 0 2237 2763 8394
Size: 2237 526 5631
Fragments arranged by size:
5631 2237 526
StuI AGG'CCT
Cuts at: 0 309 2659 7548 8394
Size: 309 2350 4889 846
Fragments arranged by size:
4889 2350 846 309
StyI C'CwwG_G
Cuts at: 0 331 2373 3233 3323 6093 6146 7334 7437
Size: 331 2042 860 90 2770 53 1188 103
Cuts at: 7437 7543 8394
Size: 106 851
Fragments arranged by size:
2770 2042 1188 860 851 331 106 103
90 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 794 1326 2527 5176 6086 6634 6979
Size: 397 397 532 1201 2649 910 548 345
Cuts at: 6979 8394
Size: 1415
Fragments arranged by size:
2649 1415 1201 910 548 532 397 397
345
TatI w'GTAC_w
Cuts at: 0 219 3837 4171 4501 5182 5197 5275 6819
Size: 219 3618 334 330 681 15 78 1544
Cuts at: 6819 7212 8394
Size: 393 1182
Fragments arranged by size:
3618 1544 1182 681 393 334 330 219
78 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 2525 2557 3598 3630 4408 4440
Size: 1170 32 1323 32 1041 32 778 32
Cuts at: 4440 7032 7064 8394
Size: 2592 32 1330
Fragments arranged by size:
2592 1330 1323 1170 1041 778 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 7610 8394
Size: 7610 784
XbaI T'CTAG_A
Cuts at: 0 6933 8394
Size: 6933 1461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 2460 2475 2746 8394
Size: 15 2445 15 271 5648
Fragments arranged by size:
5648 2445 271 15 15
XhoI C'TCGA_G
Cuts at: 0 452 473 881 8394
Size: 452 21 408 7513
Fragments arranged by size:
7513 452 408 21
XmnI GAAnn'nnTTC
Cuts at: 0 7579 7847 8394
Size: 7579 268 547
Fragments arranged by size:
7579 547 268
ZraI GAC'GTC
Cuts at: 0 5 302 8394
Size: 5 297 8092
Fragments arranged by size:
8092 297 5
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AloI ApaI ApaLI AscI AseI
AsiSI AvrII BcgI BglI BsiEI BsiWI BspEI BssHII
BstBI ClaI DraIII DrdI EagI EciI EcoRV FseI
FspI FspAI MluI NotI NruI PacI PasI PmeI
PsiI PspOMI PvuI RsrII SacII SanDI SexAI SfiI
SgfI SgrAI SmaI SnaBI SrfI SwaI XmaI