Restriction map for
pF1KA0901
(Linear) (Six-base) MAPSORT of: KIAA0901.seq Check: 1176 from: 1 to: 3645
KIAA0901.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1112 2300 3645
Size: 1112 1188 1345
Fragments arranged by size:
1345 1188 1112
AccI GT'mk_AC
Cuts at: 0 3452 3645
Size: 3452 193
Acc65I G'GTAC_C
Cuts at: 0 1506 3645
Size: 1506 2139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 880 1196 1250 3645
Size: 260 620 316 54 2395
Fragments arranged by size:
2395 620 316 260 54
AflIII A'CryG_T
Cuts at: 0 1973 3645
Size: 1973 1672
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 526 558 3645
Size: 479 32 15 32 3087
Fragments arranged by size:
3087 479 32 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 568 1600 2489 3645
Size: 568 1032 889 1156
Fragments arranged by size:
1156 1032 889 568
ApaI G_GGCC'C
Cuts at: 0 2123 2448 3645
Size: 2123 325 1197
Fragments arranged by size:
2123 1197 325
ApaLI G'TGCA_C
Cuts at: 0 760 1733 3645
Size: 760 973 1912
Fragments arranged by size:
1912 973 760
ApoI r'AATT_y
Cuts at: 0 286 3498 3645
Size: 286 3212 147
Fragments arranged by size:
3212 286 147
AvaI C'yCGr_G
Cuts at: 0 994 1667 3072 3645
Size: 994 673 1405 573
Fragments arranged by size:
1405 994 673 573
AvrII C'CTAG_G
Cuts at: 0 2581 3645
Size: 2581 1064
BamHI G'GATC_C
Cuts at: 0 1925 2238 3645
Size: 1925 313 1407
Fragments arranged by size:
1925 1407 313
BanI G'GyrC_C
Cuts at: 0 1240 1506 2029 2056 2562 3043 3645
Size: 1240 266 523 27 506 481 602
Fragments arranged by size:
1240 602 523 506 481 266 27
BanII G_rGCy'C
Cuts at: 0 1298 1397 2123 2448 3645
Size: 1298 99 726 325 1197
Fragments arranged by size:
1298 1197 726 325 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 2525 3645
Size: 200 2325 1120
Fragments arranged by size:
2325 1120 200
BbvCI CC'TCA_GC
Cuts at: 0 1007 3520 3645
Size: 1007 2513 125
Fragments arranged by size:
2513 1007 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2052 2086 3255 3289 3645
Size: 2052 34 1169 34 356
Fragments arranged by size:
2052 1169 356 34 34
BclI T'GATC_A
Cuts at: 0 2022 3297 3645
Size: 2022 1275 348
Fragments arranged by size:
2022 1275 348
BglI GCCn_nnn'nGGC
Cuts at: 0 556 1078 1597 2072 2758 3645
Size: 556 522 519 475 686 887
Fragments arranged by size:
887 686 556 522 519 475
BlpI GC'TnA_GC
Cuts at: 0 1601 2591 3645
Size: 1601 990 1054
Fragments arranged by size:
1601 1054 990
Bme1580I G_kGCm'C
Cuts at: 0 764 1245 1260 1737 2089 2123 2448 3046
Size: 764 481 15 477 352 34 325 598
Cuts at: 3046 3645
Size: 599
Fragments arranged by size:
764 599 598 481 477 352 325 34
15
BmgBI CAC'GTC
Cuts at: 0 975 3384 3645
Size: 975 2409 261
Fragments arranged by size:
2409 975 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 462 797 1712 1985 3131 3645
Size: 54 408 335 915 273 1146 514
Fragments arranged by size:
1146 915 514 408 335 273 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1165 1249 1561 2520 2660 3020 3263
Size: 63 1102 84 312 959 140 360 243
Cuts at: 3263 3645
Size: 382
Fragments arranged by size:
1102 959 382 360 312 243 140 84
63
Bpu10I CC'TnA_GC
Cuts at: 0 326 1007 3520 3645
Size: 326 681 2513 125
Fragments arranged by size:
2513 681 326 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1312 3645
Size: 253 1059 2333
Fragments arranged by size:
2333 1059 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 1205 1321 2214 2393 2458 3645
Size: 1205 116 893 179 65 1187
Fragments arranged by size:
1205 1187 893 179 116 65
BsaAI yAC'GTr
Cuts at: 0 690 1632 3645
Size: 690 942 2013
Fragments arranged by size:
2013 942 690
BsaHI Gr'CG_yC
Cuts at: 0 1181 3645
Size: 1181 2464
BsaWI w'CCGG_w
Cuts at: 0 608 731 2841 3645
Size: 608 123 2110 804
Fragments arranged by size:
2110 804 608 123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 1684 1714 3645
Size: 526 30 1128 30 1931
Fragments arranged by size:
1931 1128 526 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1366 1381 1390 1558 2833 2875 2888 2959
Size: 1366 15 9 168 1275 42 13 71
Cuts at: 2959 2972 3043 3053 3232 3645
Size: 13 71 10 179 413
Fragments arranged by size:
1366 1275 413 179 168 71 71 42
15 13 13 10 9
BseYI C'CCAG_C
Cuts at: 0 650 1091 1511 1548 2244 2799 2925 3645
Size: 650 441 420 37 696 555 126 720
Fragments arranged by size:
720 696 650 555 441 420 126 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1660 1996 2213 2364 3130 3645
Size: 955 705 336 217 151 766 515
Fragments arranged by size:
955 766 705 515 336 217 151
BsiEI CG_ry'CG
Cuts at: 0 2076 3645
Size: 2076 1569
BsiHKAI G_wGCw'C
Cuts at: 0 764 1281 1406 1737 1781 3079 3645
Size: 764 517 125 331 44 1298 566
Fragments arranged by size:
1298 764 566 517 331 125 44
Bsp1286I G_dGCh'C
Cuts at: 0 764 1245 1260 1281 1298 1397 1406 1737
Size: 764 481 15 21 17 99 9 331
Cuts at: 1737 1781 2089 2123 2448 3046 3079 3645
Size: 44 308 34 325 598 33 566
Fragments arranged by size:
764 598 566 481 331 325 308 99
44 34 33 21 17 15 9
BspEI T'CCGG_A
Cuts at: 0 608 731 2841 3645
Size: 608 123 2110 804
Fragments arranged by size:
2110 804 608 123
BspHI T'CATG_A
Cuts at: 0 2507 3645
Size: 2507 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1112 2300 3375 3645
Size: 1112 1188 1075 270
Fragments arranged by size:
1188 1112 1075 270
BsrBI CCG'CTC
Cuts at: 0 125 330 2595 3645
Size: 125 205 2265 1050
Fragments arranged by size:
2265 1050 205 125
BsrDI GCAATG_nn'
Cuts at: 0 191 291 956 2174 3645
Size: 191 100 665 1218 1471
Fragments arranged by size:
1471 1218 665 191 100
BsrFI r'CCGG_y
Cuts at: 0 10 3645
Size: 10 3635
BssSI C'ACGA_G
Cuts at: 0 2606 3645
Size: 2606 1039
BstAPI GCAn_nnn'nTGC
Cuts at: 0 978 1741 3645
Size: 978 763 1904
Fragments arranged by size:
1904 978 763
BstBI TT'CG_AA
Cuts at: 0 91 3645
Size: 91 3554
BstEII G'GTnAC_C
Cuts at: 0 2550 3645
Size: 2550 1095
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1960 3332 3397 3493 3645
Size: 1960 1372 65 96 152
Fragments arranged by size:
1960 1372 152 96 65
BstYI r'GATC_y
Cuts at: 0 222 605 1925 2238 3280 3645
Size: 222 383 1320 313 1042 365
Fragments arranged by size:
1320 1042 383 365 313 222
Bsu36I CC'TnA_GG
Cuts at: 0 71 561 574 1502 2267 3645
Size: 71 490 13 928 765 1378
Fragments arranged by size:
1378 928 765 490 71 13
BtgI C'CryG_G
Cuts at: 0 765 1331 1953 2042 2369 2425 2993 3492
Size: 765 566 622 89 327 56 568 499
Cuts at: 3492 3645
Size: 153
Fragments arranged by size:
765 622 568 566 499 327 153 89
56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2143 2464 3585 3645
Size: 2143 321 1121 60
Fragments arranged by size:
2143 1121 321 60
BtsI GCAGTG_nn'
Cuts at: 0 1473 3325 3645
Size: 1473 1852 320
Fragments arranged by size:
1852 1473 320
DraIII CAC_nnn'GTG
Cuts at: 0 1240 3645
Size: 1240 2405
DrdI GACnn_nn'nnGTC
Cuts at: 0 82 805 3645
Size: 82 723 2840
Fragments arranged by size:
2840 723 82
EaeI y'GGCC_r
Cuts at: 0 12 177 620 891 1067 1420 2073 2313
Size: 12 165 443 271 176 353 653 240
Cuts at: 2313 3474 3645
Size: 1161 171
Fragments arranged by size:
1161 653 443 353 271 240 176 171
165 12
EagI C'GGCC_G
Cuts at: 0 2073 3645
Size: 2073 1572
EarI CTCTTCn'nnn_
Cuts at: 0 410 2660 3645
Size: 410 2250 985
Fragments arranged by size:
2250 985 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 260 880 1165 1196 1249 1250 1561
Size: 63 197 620 285 31 53 1 311
Cuts at: 1561 2520 2660 3020 3263 3645
Size: 959 140 360 243 382
Fragments arranged by size:
959 620 382 360 311 285 243 197
140 63 53 31 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1768 2716 3371 3645
Size: 1768 948 655 274
Fragments arranged by size:
1768 948 655 274
EcoO109I rG'GnC_Cy
Cuts at: 0 322 366 738 798 1050 1384 2120 2444
Size: 322 44 372 60 252 334 736 324
Cuts at: 2444 2445 2530 3161 3191 3207 3288 3645
Size: 1 85 631 30 16 81 357
Fragments arranged by size:
736 631 372 357 334 324 322 252
85 81 60 44 30 16 1
EcoRI G'AATT_C
Cuts at: 0 286 3645
Size: 286 3359
EcoRV GAT'ATC
Cuts at: 0 3131 3645
Size: 3131 514
HaeII r_GCGC'y
Cuts at: 0 1566 1754 3645
Size: 1566 188 1891
Fragments arranged by size:
1891 1566 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 1125 1157 2713 2745 3045 3077
Size: 896 32 197 32 1556 32 300 32
Cuts at: 3077 3138 3170 3645
Size: 61 32 475
Fragments arranged by size:
1556 896 475 300 197 61 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2103 3645
Size: 2103 1542
KpnI G_GTAC'C
Cuts at: 0 1510 3645
Size: 1510 2135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1715 3510 3645
Size: 248 1467 1795 135
Fragments arranged by size:
1795 1467 248 135
MscI TGG'CCA
Cuts at: 0 622 1422 3476 3645
Size: 622 800 2054 169
Fragments arranged by size:
2054 800 622 169
MslI CAynn'nnrTG
Cuts at: 0 916 1958 2018 3645
Size: 916 1042 60 1627
Fragments arranged by size:
1627 1042 916 60
MspA1I CmG'CkG
Cuts at: 0 1011 2215 2244 2701 3241 3513 3645
Size: 1011 1204 29 457 540 272 132
Fragments arranged by size:
1204 1011 540 457 272 132 29
NcoI C'CATG_G
Cuts at: 0 2042 2369 2993 3492 3645
Size: 2042 327 624 499 153
Fragments arranged by size:
2042 624 499 327 153
NspI r_CATG'y
Cuts at: 0 681 1918 1977 3483 3645
Size: 681 1237 59 1506 162
Fragments arranged by size:
1506 1237 681 162 59
PasI CC'CwG_GG
Cuts at: 0 1388 3645
Size: 1388 2257
PciI A'CATG_T
Cuts at: 0 1973 3645
Size: 1973 1672
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 3348 3645
Size: 186 3162 297
Fragments arranged by size:
3162 297 186
PfoI T'CCnGG_A
Cuts at: 0 314 359 636 3645
Size: 314 45 277 3009
Fragments arranged by size:
3009 314 277 45
PmlI CAC'GTG
Cuts at: 0 690 3645
Size: 690 2955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 3645
Size: 526 32 3087
Fragments arranged by size:
3087 526 32
PpuMI rG'GwC_Cy
Cuts at: 0 738 798 1050 1384 2530 3161 3645
Size: 738 60 252 334 1146 631 484
Fragments arranged by size:
1146 738 631 484 334 252 60
PspOMI G'GGCC_C
Cuts at: 0 2119 2444 3645
Size: 2119 325 1201
Fragments arranged by size:
2119 1201 325
PspXI vC'TCGA_Gb
Cuts at: 0 994 3072 3645
Size: 994 2078 573
Fragments arranged by size:
2078 994 573
PstI C_TGCA'G
Cuts at: 0 3372 3645
Size: 3372 273
PvuII CAG'CTG
Cuts at: 0 1011 2215 2701 3241 3645
Size: 1011 1204 486 540 404
Fragments arranged by size:
1204 1011 540 486 404
SanDI GG'GwC_CC
Cuts at: 0 1050 3645
Size: 1050 2595
SapI GCTCTTCn'nnn_
Cuts at: 0 410 3645
Size: 410 3235
SexAI A'CCwGG_T
Cuts at: 0 929 1236 3645
Size: 929 307 2409
Fragments arranged by size:
2409 929 307
SfcI C'TryA_G
Cuts at: 0 1429 2951 3368 3645
Size: 1429 1522 417 277
Fragments arranged by size:
1522 1429 417 277
SmaI CCC'GGG
Cuts at: 0 1669 3645
Size: 1669 1976
SmlI C'TyrA_G
Cuts at: 0 232 994 1291 3072 3645
Size: 232 762 297 1781 573
Fragments arranged by size:
1781 762 573 297 232
SphI G_CATG'C
Cuts at: 0 1918 3645
Size: 1918 1727
SspI AAT'ATT
Cuts at: 0 102 3645
Size: 102 3543
StuI AGG'CCT
Cuts at: 0 633 865 3377 3562 3645
Size: 633 232 2512 185 83
Fragments arranged by size:
2512 633 232 185 83
StyI C'CwwG_G
Cuts at: 0 894 900 922 1055 2042 2369 2394 2581
Size: 894 6 22 133 987 327 25 187
Cuts at: 2581 2993 3164 3492 3645
Size: 412 171 328 153
Fragments arranged by size:
987 894 412 328 327 187 171 153
133 25 22 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2119 2302 3417 3645
Size: 2119 183 1115 228
Fragments arranged by size:
2119 1115 228 183
TatI w'GTAC_w
Cuts at: 0 470 3139 3645
Size: 470 2669 506
Fragments arranged by size:
2669 506 470
Tth111I GACn'n_nGTC
Cuts at: 0 3021 3645
Size: 3021 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1415 1906 1957 2544 3499 3616 3645
Size: 1062 353 491 51 587 955 117 29
Fragments arranged by size:
1062 955 587 491 353 117 51 29
XhoI C'TCGA_G
Cuts at: 0 994 3072 3645
Size: 994 2078 573
Fragments arranged by size:
2078 994 573
XmaI C'CCGG_G
Cuts at: 0 1667 3645
Size: 1667 1978
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuII SanDI SapI SexAI
SfcI SmaI SmlI SphI SspI StuI StyI TaqII
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AscI AseI AsiSI BaeI BbeI BciVI BfrBI BglII
BmtI BplI BsaBI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraI EciI EcoICRI FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PshAI PsiI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SwaI TstI XbaI XmnI ZraI