Restriction map for
pF1KA0904
(Linear) (Six-base) MAPSORT of: KIAA0904.seq Check: 195 from: 1 to: 4443
KIAA0904.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3926 4443
Size: 3926 517
AatII G_ACGT'C
Cuts at: 0 3147 4443
Size: 3147 1296
AccI GT'mk_AC
Cuts at: 0 2943 4443
Size: 2943 1500
Acc65I G'GTAC_C
Cuts at: 0 2699 4443
Size: 2699 1744
AclI AA'CG_TT
Cuts at: 0 1876 2733 4443
Size: 1876 857 1710
Fragments arranged by size:
1876 1710 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1409 2284 2678 3554 3917 4255 4443
Size: 589 820 875 394 876 363 338 188
Fragments arranged by size:
876 875 820 589 394 363 338 188
AflIII A'CryG_T
Cuts at: 0 1984 4443
Size: 1984 2459
AleI CACnn'nnGTG
Cuts at: 0 2351 4443
Size: 2351 2092
AlwNI CAG_nnn'CTG
Cuts at: 0 1225 1378 3447 4080 4443
Size: 1225 153 2069 633 363
Fragments arranged by size:
2069 1225 633 363 153
ApaI G_GGCC'C
Cuts at: 0 3722 4357 4443
Size: 3722 635 86
Fragments arranged by size:
3722 635 86
ApaLI G'TGCA_C
Cuts at: 0 2485 3024 4443
Size: 2485 539 1419
Fragments arranged by size:
2485 1419 539
ApoI r'AATT_y
Cuts at: 0 1419 1797 2108 2559 2953 4443
Size: 1419 378 311 451 394 1490
Fragments arranged by size:
1490 1419 451 394 378 311
AvaI C'yCGr_G
Cuts at: 0 356 711 765 807 4443
Size: 356 355 54 42 3636
Fragments arranged by size:
3636 356 355 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1820 1853 4443
Size: 1820 33 2590
Fragments arranged by size:
2590 1820 33
BanI G'GyrC_C
Cuts at: 0 2419 2699 4443
Size: 2419 280 1744
Fragments arranged by size:
2419 1744 280
BanII G_rGCy'C
Cuts at: 0 667 880 2024 3722 3779 4357 4443
Size: 667 213 1144 1698 57 578 86
Fragments arranged by size:
1698 1144 667 578 213 86 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 1834 2142 4358 4443
Size: 553 1281 308 2216 85
Fragments arranged by size:
2216 1281 553 308 85
BbvCI CC'TCA_GC
Cuts at: 0 2081 3260 4241 4443
Size: 2081 1179 981 202
Fragments arranged by size:
2081 1179 981 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 347 4443
Size: 313 34 4096
Fragments arranged by size:
4096 313 34
BclI T'GATC_A
Cuts at: 0 2840 4443
Size: 2840 1603
BglI GCCn_nnn'nGGC
Cuts at: 0 3717 4443
Size: 3717 726
BglII A'GATC_T
Cuts at: 0 898 2034 4443
Size: 898 1136 2409
Fragments arranged by size:
2409 1136 898
BlpI GC'TnA_GC
Cuts at: 0 2828 4443
Size: 2828 1615
Bme1580I G_kGCm'C
Cuts at: 0 193 581 2489 3028 3477 3722 4147 4357
Size: 193 388 1908 539 449 245 425 210
Cuts at: 4357 4443
Size: 86
Fragments arranged by size:
1908 539 449 425 388 245 210 193
86
BmgBI CAC'GTC
Cuts at: 0 1987 4443
Size: 1987 2456
BmrI ACTGGGnnnn_n'
Cuts at: 0 965 1037 2163 3550 4325 4356 4443
Size: 965 72 1126 1387 775 31 87
Fragments arranged by size:
1387 1126 965 775 87 72 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1210 3410 4443
Size: 1210 2200 1033
Fragments arranged by size:
2200 1210 1033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1931 2000 2039 2693 3074 3700 4443
Size: 393 1538 69 39 654 381 626 743
Fragments arranged by size:
1538 743 654 626 393 381 69 39
Bpu10I CC'TnA_GC
Cuts at: 0 1009 1310 2081 3260 3385 3773 4200 4241
Size: 1009 301 771 1179 125 388 427 41
Cuts at: 4241 4329 4443
Size: 88 114
Fragments arranged by size:
1179 1009 771 427 388 301 125 114
88 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1817 3660 3960 4443
Size: 1817 1843 300 483
Fragments arranged by size:
1843 1817 483 300
BsaI GGTCTCn'nnnn_
Cuts at: 0 1025 1564 2094 3505 3936 4267 4443
Size: 1025 539 530 1411 431 331 176
Fragments arranged by size:
1411 1025 539 530 431 331 176
BsaHI Gr'CG_yC
Cuts at: 0 2940 3144 4443
Size: 2940 204 1299
Fragments arranged by size:
2940 1299 204
BsaWI w'CCGG_w
Cuts at: 0 434 587 4443
Size: 434 153 3856
Fragments arranged by size:
3856 434 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 556 582 586 612 1614 1644
Size: 232 30 294 26 4 26 1002 30
Cuts at: 1644 1866 1896 2240 2270 3406 3436 4443
Size: 222 30 344 30 1136 30 1007
Fragments arranged by size:
1136 1007 1002 344 294 232 222 30
30 30 30 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 59 481 1543 3077 3541 3763 4443
Size: 59 422 1062 1534 464 222 680
Fragments arranged by size:
1534 1062 680 464 422 222 59
BseYI C'CCAG_C
Cuts at: 0 3249 3292 3494 3855 3890 4299 4443
Size: 3249 43 202 361 35 409 144
Fragments arranged by size:
3249 409 361 202 144 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 4177 4443
Size: 307 3870 266
Fragments arranged by size:
3870 307 266
BsiEI CG_ry'CG
Cuts at: 0 318 928 4443
Size: 318 610 3515
Fragments arranged by size:
3515 610 318
BsiHKAI G_wGCw'C
Cuts at: 0 2024 2489 3028 3932 4443
Size: 2024 465 539 904 511
Fragments arranged by size:
2024 904 539 511 465
BsmBI CGTCTCn'nnnn_
Cuts at: 0 915 4443
Size: 915 3528
Bsp1286I G_dGCh'C
Cuts at: 0 193 581 667 880 2024 2489 3028 3477
Size: 193 388 86 213 1144 465 539 449
Cuts at: 3477 3722 3779 3932 4147 4357 4443
Size: 245 57 153 215 210 86
Fragments arranged by size:
1144 539 465 449 388 245 215 213
210 193 153 86 86 57
BspHI T'CATG_A
Cuts at: 0 2516 4443
Size: 2516 1927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 3371 3926 4443
Size: 483 2888 555 517
Fragments arranged by size:
2888 555 517 483
BsrGI T'GTAC_A
Cuts at: 0 1812 4443
Size: 1812 2631
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1225 3550 4443
Size: 1225 2325 893
Fragments arranged by size:
2325 1225 893
BstBI TT'CG_AA
Cuts at: 0 460 4443
Size: 460 3983
BstEII G'GTnAC_C
Cuts at: 0 165 869 4443
Size: 165 704 3574
Fragments arranged by size:
3574 704 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 2876 4443
Size: 156 2720 1567
Fragments arranged by size:
2720 1567 156
BstYI r'GATC_y
Cuts at: 0 655 898 1120 2034 3009 4443
Size: 655 243 222 914 975 1434
Fragments arranged by size:
1434 975 914 655 243 222
Bsu36I CC'TnA_GG
Cuts at: 0 3213 4443
Size: 3213 1230
BtgI C'CryG_G
Cuts at: 0 3515 4443
Size: 3515 928
BtsI GCAGTG_nn'
Cuts at: 0 3356 4443
Size: 3356 1087
ClaI AT'CG_AT
Cuts at: 0 424 4443
Size: 424 4019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3687 3722 4443
Size: 3687 35 721
Fragments arranged by size:
3687 721 35
DraIII CAC_nnn'GTG
Cuts at: 0 4215 4443
Size: 4215 228
EaeI y'GGCC_r
Cuts at: 0 726 2214 3912 4443
Size: 726 1488 1698 531
Fragments arranged by size:
1698 1488 726 531
EarI CTCTTCn'nnn_
Cuts at: 0 1111 1589 1682 1949 2654 3167 3757 4416
Size: 1111 478 93 267 705 513 590 659
Cuts at: 4416 4443
Size: 27
Fragments arranged by size:
1111 705 659 590 513 478 267 93
27
EcoICRI GAG'CTC
Cuts at: 0 2022 4443
Size: 2022 2421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 589 1409 1931 2000 2039 2284 2678
Size: 393 196 820 522 69 39 245 394
Cuts at: 2678 2693 3074 3554 3700 3917 4255 4443
Size: 15 381 480 146 217 338 188
Fragments arranged by size:
820 522 480 394 393 381 338 245
217 196 188 146 69 39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 810 885 915 4007 4443
Size: 810 75 30 3092 436
Fragments arranged by size:
3092 810 436 75 30
EcoO109I rG'GnC_Cy
Cuts at: 0 3718 3719 3767 4094 4176 4194 4353 4393
Size: 3718 1 48 327 82 18 159 40
Cuts at: 4393 4443
Size: 50
Fragments arranged by size:
3718 327 159 82 50 48 40 18
1
EcoRI G'AATT_C
Cuts at: 0 2953 4443
Size: 2953 1490
EcoRV GAT'ATC
Cuts at: 0 225 440 4443
Size: 225 215 4003
Fragments arranged by size:
4003 225 215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 968 1960 3844 4443
Size: 968 992 1884 599
Fragments arranged by size:
1884 992 968 599
HaeII r_GCGC'y
Cuts at: 0 3839 4443
Size: 3839 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 232 246 264 424 456 556 582
Size: 214 18 14 18 160 32 100 26
Cuts at: 582 588 614 694 726 1017 1049 1441 1473
Size: 6 26 80 32 291 32 392 32
Cuts at: 1473 1524 1556 1971 2003 2994 3026 4443
Size: 51 32 415 32 991 32 1417
Fragments arranged by size:
1417 991 415 392 291 214 160 100
80 51 32 32 32 32 32 32
32 26 26 18 18 14 6
HincII GTy'rAC
Cuts at: 0 2361 4443
Size: 2361 2082
HindIII A'AGCT_T
Cuts at: 0 3593 4443
Size: 3593 850
HpaI GTT'AAC
Cuts at: 0 2361 4443
Size: 2361 2082
KpnI G_GTAC'C
Cuts at: 0 2703 4443
Size: 2703 1740
MfeI C'AATT_G
Cuts at: 0 1592 4443
Size: 1592 2851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1009 1427 3449 4443
Size: 263 746 418 2022 994
Fragments arranged by size:
2022 994 746 418 263
MscI TGG'CCA
Cuts at: 0 728 2216 3914 4443
Size: 728 1488 1698 529
Fragments arranged by size:
1698 1488 728 529
MslI CAynn'nnrTG
Cuts at: 0 2351 4443
Size: 2351 2092
MspA1I CmG'CkG
Cuts at: 0 959 1222 1849 2872 3031 3330 3408 3447
Size: 959 263 627 1023 159 299 78 39
Cuts at: 3447 3610 3648 4443
Size: 163 38 795
Fragments arranged by size:
1023 959 795 627 299 263 163 159
78 39 38
NcoI C'CATG_G
Cuts at: 0 3515 4443
Size: 3515 928
NspI r_CATG'y
Cuts at: 0 3000 3618 4270 4443
Size: 3000 618 652 173
Fragments arranged by size:
3000 652 618 173
PasI CC'CwG_GG
Cuts at: 0 186 4443
Size: 186 4257
PflMI CCAn_nnn'nTGG
Cuts at: 0 4080 4443
Size: 4080 363
PfoI T'CCnGG_A
Cuts at: 0 658 795 4443
Size: 658 137 3648
Fragments arranged by size:
3648 658 137
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 4443
Size: 1110 32 3301
Fragments arranged by size:
3301 1110 32
PpuMI rG'GwC_Cy
Cuts at: 0 4094 4176 4194 4443
Size: 4094 82 18 249
Fragments arranged by size:
4094 249 82 18
PshAI GACnn'nnGTC
Cuts at: 0 293 743 927 4443
Size: 293 450 184 3516
Fragments arranged by size:
3516 450 293 184
PspOMI G'GGCC_C
Cuts at: 0 3718 4353 4443
Size: 3718 635 90
Fragments arranged by size:
3718 635 90
PspXI vC'TCGA_Gb
Cuts at: 0 765 4443
Size: 765 3678
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3026 3058 4443
Size: 3026 32 1385
Fragments arranged by size:
3026 1385 32
PstI C_TGCA'G
Cuts at: 0 1236 2976 3371 4443
Size: 1236 1740 395 1072
Fragments arranged by size:
1740 1236 1072 395
PvuII CAG'CTG
Cuts at: 0 1222 1849 2872 3031 3330 3408 3447 3610
Size: 1222 627 1023 159 299 78 39 163
Cuts at: 3610 3648 4443
Size: 38 795
Fragments arranged by size:
1222 1023 795 627 299 163 159 78
39 38
SacI G_AGCT'C
Cuts at: 0 2024 4443
Size: 2024 2419
SanDI GG'GwC_CC
Cuts at: 0 4194 4443
Size: 4194 249
SapI GCTCTTCn'nnn_
Cuts at: 0 1682 3167 4443
Size: 1682 1485 1276
Fragments arranged by size:
1682 1485 1276
ScaI AGT'ACT
Cuts at: 0 1740 3225 3955 4443
Size: 1740 1485 730 488
Fragments arranged by size:
1740 1485 730 488
SfcI C'TryA_G
Cuts at: 0 882 912 1232 2972 3043 3367 4443
Size: 882 30 320 1740 71 324 1076
Fragments arranged by size:
1740 1076 882 324 320 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3717 4443
Size: 3717 726
SmaI CCC'GGG
Cuts at: 0 358 4443
Size: 358 4085
SmlI C'TyrA_G
Cuts at: 0 765 807 1832 3639 3939 4443
Size: 765 42 1025 1807 300 504
Fragments arranged by size:
1807 1025 765 504 300 42
SpeI A'CTAG_T
Cuts at: 0 2253 4443
Size: 2253 2190
StuI AGG'CCT
Cuts at: 0 3308 3937 4340 4443
Size: 3308 629 403 103
Fragments arranged by size:
3308 629 403 103
StyI C'CwwG_G
Cuts at: 0 1259 2231 3515 4067 4119 4443
Size: 1259 972 1284 552 52 324
Fragments arranged by size:
1284 1259 972 552 324 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 397 633 810 984 4443
Size: 290 107 236 177 174 3459
Fragments arranged by size:
3459 290 236 177 174 107
TatI w'GTAC_w
Cuts at: 0 1738 1812 3223 3953 4443
Size: 1738 74 1411 730 490
Fragments arranged by size:
1738 1411 730 490 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1989 2021 2151 2183 2240 2272
Size: 337 32 1620 32 130 32 57 32
Cuts at: 2272 2566 2598 4443
Size: 294 32 1845
Fragments arranged by size:
1845 1620 337 294 130 57 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3968 4443
Size: 3968 475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2820 4443
Size: 2820 1623
XhoI C'TCGA_G
Cuts at: 0 765 807 4443
Size: 765 42 3636
Fragments arranged by size:
3636 765 42
XmaI C'CCGG_G
Cuts at: 0 356 4443
Size: 356 4087
XmnI GAAnn'nnTTC
Cuts at: 0 1943 4443
Size: 1943 2500
ZraI GAC'GTC
Cuts at: 0 3145 4443
Size: 3145 1298
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrGI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SanDI SapI ScaI SfcI SfiI SmaI SmlI
SpeI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI AscI AseI
AsiSI AvrII BamHI BbeI BciVI BfrBI BmtI BsaAI
BsaBI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstZ17I BtgZI DraI DrdI EagI EciI FseI
FspI FspAI KasI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PsiI PvuI RsrII SacII SalI SbfI SexAI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI