Restriction map for
pF1KA0905
(Linear) (Six-base) MAPSORT of: KIAA0905.seq Check: 2124 from: 1 to: 3660
KIAA0905.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 262 1801 3660
Size: 262 1539 1859
Fragments arranged by size:
1859 1539 262
Acc65I G'GTAC_C
Cuts at: 0 203 2601 3660
Size: 203 2398 1059
Fragments arranged by size:
2398 1059 203
AclI AA'CG_TT
Cuts at: 0 3444 3660
Size: 3444 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1318 1625 2134 2876 3104 3380 3576
Size: 1168 150 307 509 742 228 276 196
Cuts at: 3576 3660
Size: 84
Fragments arranged by size:
1168 742 509 307 276 228 196 150
84
AfeI AGC'GCT
Cuts at: 0 3385 3660
Size: 3385 275
AgeI A'CCGG_T
Cuts at: 0 707 3660
Size: 707 2953
AhdI GACnn_n'nnGTC
Cuts at: 0 3093 3660
Size: 3093 567
AleI CACnn'nnGTG
Cuts at: 0 912 1891 3660
Size: 912 979 1769
Fragments arranged by size:
1769 979 912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 116 2085 2711 3660
Size: 116 1969 626 949
Fragments arranged by size:
1969 949 626 116
AlwNI CAG_nnn'CTG
Cuts at: 0 817 2157 2452 3660
Size: 817 1340 295 1208
Fragments arranged by size:
1340 1208 817 295
ApoI r'AATT_y
Cuts at: 0 1106 1402 1654 1990 2402 3660
Size: 1106 296 252 336 412 1258
Fragments arranged by size:
1258 1106 412 336 296 252
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2585 2618 3660
Size: 2585 33 1042
Fragments arranged by size:
2585 1042 33
BanI G'GyrC_C
Cuts at: 0 203 2601 3256 3660
Size: 203 2398 655 404
Fragments arranged by size:
2398 655 404 203
BanII G_rGCy'C
Cuts at: 0 46 1597 2880 3270 3660
Size: 46 1551 1283 390 390
Fragments arranged by size:
1551 1283 390 390 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 3140 3660
Size: 3140 520
BbvCI CC'TCA_GC
Cuts at: 0 1315 2684 3660
Size: 1315 1369 976
Fragments arranged by size:
1369 1315 976
BciVI GTATCCnnnnn_n'
Cuts at: 0 1474 2657 3660
Size: 1474 1183 1003
Fragments arranged by size:
1474 1183 1003
BfrBI ATG'CAT
Cuts at: 0 642 2535 3564 3660
Size: 642 1893 1029 96
Fragments arranged by size:
1893 1029 642 96
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1546 3265 3660
Size: 34 1512 1719 395
Fragments arranged by size:
1719 1512 395 34
BglII A'GATC_T
Cuts at: 0 1494 3660
Size: 1494 2166
BlpI GC'TnA_GC
Cuts at: 0 89 1124 1253 3660
Size: 89 1035 129 2407
Fragments arranged by size:
2407 1035 129 89
Bme1580I G_kGCm'C
Cuts at: 0 3261 3660
Size: 3261 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 560 2768 3087 3660
Size: 364 196 2208 319 573
Fragments arranged by size:
2208 573 364 319 196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2062 3660
Size: 2062 1598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 344 1691 2564 2793 2867 2978 3110
Size: 278 66 1347 873 229 74 111 132
Cuts at: 3110 3660
Size: 550
Fragments arranged by size:
1347 873 550 278 229 132 111 74
66
Bpu10I CC'TnA_GC
Cuts at: 0 1315 2276 2684 3660
Size: 1315 961 408 976
Fragments arranged by size:
1315 976 961 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 2042 2108 2186 3660
Size: 148 1894 66 78 1474
Fragments arranged by size:
1894 1474 148 78 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 3592 3660
Size: 3592 68
BsaWI w'CCGG_w
Cuts at: 0 707 3660
Size: 707 2953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1529 1559 2363 2393 2408 2438 3442 3472
Size: 1529 30 804 30 15 30 1004 30
Cuts at: 3472 3660
Size: 188
Fragments arranged by size:
1529 1004 804 188 30 30 30 30
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 180 1603 2690 2822 3660
Size: 180 1423 1087 132 838
Fragments arranged by size:
1423 1087 838 180 132
BseYI C'CCAG_C
Cuts at: 0 2639 3651 3660
Size: 2639 1012 9
Fragments arranged by size:
2639 1012 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 2102 3316 3660
Size: 1366 736 1214 344
Fragments arranged by size:
1366 1214 736 344
BsiHKAI G_wGCw'C
Cuts at: 0 1628 2367 2880 3347 3660
Size: 1628 739 513 467 313
Fragments arranged by size:
1628 739 513 467 313
BsiWI C'GTAC_G
Cuts at: 0 2408 3660
Size: 2408 1252
BsmI GAATG_Cn'
Cuts at: 0 123 644 1238 3660
Size: 123 521 594 2422
Fragments arranged by size:
2422 594 521 123
Bsp1286I G_dGCh'C
Cuts at: 0 46 1597 1628 2367 2880 3261 3270 3347
Size: 46 1551 31 739 513 381 9 77
Cuts at: 3347 3660
Size: 313
Fragments arranged by size:
1551 739 513 381 313 77 46 31
9
BspHI T'CATG_A
Cuts at: 0 3071 3660
Size: 3071 589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 262 1801 3660
Size: 262 1539 1859
Fragments arranged by size:
1859 1539 262
BsrDI GCAATG_nn'
Cuts at: 0 518 2678 3515 3660
Size: 518 2160 837 145
Fragments arranged by size:
2160 837 518 145
BsrFI r'CCGG_y
Cuts at: 0 707 3660
Size: 707 2953
BssSI C'ACGA_G
Cuts at: 0 1454 3660
Size: 1454 2206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1344 3303 3660
Size: 1344 1959 357
Fragments arranged by size:
1959 1344 357
BstBI TT'CG_AA
Cuts at: 0 1170 3660
Size: 1170 2490
BstEII G'GTnAC_C
Cuts at: 0 710 3085 3660
Size: 710 2375 575
Fragments arranged by size:
2375 710 575
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1800 2268 3651 3660
Size: 1800 468 1383 9
Fragments arranged by size:
1800 1383 468 9
BstYI r'GATC_y
Cuts at: 0 450 588 732 813 1494 2154 3372 3660
Size: 450 138 144 81 681 660 1218 288
Fragments arranged by size:
1218 681 660 450 288 144 138 81
BtgI C'CryG_G
Cuts at: 0 2207 2499 3189 3660
Size: 2207 292 690 471
Fragments arranged by size:
2207 690 471 292
BtsI GCAGTG_nn'
Cuts at: 0 1886 1898 2902 2926 3660
Size: 1886 12 1004 24 734
Fragments arranged by size:
1886 1004 734 24 12
DraIII CAC_nnn'GTG
Cuts at: 0 760 911 1278 1579 3660
Size: 760 151 367 301 2081
Fragments arranged by size:
2081 760 367 301 151
EaeI y'GGCC_r
Cuts at: 0 570 1784 1802 2454 3177 3660
Size: 570 1214 18 652 723 483
Fragments arranged by size:
1214 723 652 570 483 18
EarI CTCTTCn'nnn_
Cuts at: 0 198 1601 2802 3660
Size: 198 1403 1201 858
Fragments arranged by size:
1403 1201 858 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2882 3660
Size: 2882 778
EcoICRI GAG'CTC
Cuts at: 0 2878 3660
Size: 2878 782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 344 1168 1318 1625 1691 2134 2564
Size: 278 66 824 150 307 66 443 430
Cuts at: 2564 2793 2867 2876 2978 3104 3110 3380 3576
Size: 229 74 9 102 126 6 270 196
Cuts at: 3576 3660
Size: 84
Fragments arranged by size:
824 443 430 307 278 270 229 196
150 126 102 84 74 66 66 9
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2381 3660
Size: 2381 1279
EcoO109I rG'GnC_Cy
Cuts at: 0 223 2968 3660
Size: 223 2745 692
Fragments arranged by size:
2745 692 223
EcoRV GAT'ATC
Cuts at: 0 510 3660
Size: 510 3150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1455 2156 3164 3660
Size: 1455 701 1008 496
Fragments arranged by size:
1455 1008 701 496
HaeII r_GCGC'y
Cuts at: 0 3387 3660
Size: 3387 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 717 749 1808 1840 2106 2138
Size: 157 32 528 32 1059 32 266 32
Cuts at: 2138 3079 3111 3660
Size: 941 32 549
Fragments arranged by size:
1059 941 549 528 266 157 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1038 1758 3660
Size: 1038 720 1902
Fragments arranged by size:
1902 1038 720
HindIII A'AGCT_T
Cuts at: 0 1042 3660
Size: 1042 2618
KpnI G_GTAC'C
Cuts at: 0 207 2605 3660
Size: 207 2398 1055
Fragments arranged by size:
2398 1055 207
MfeI C'AATT_G
Cuts at: 0 94 794 3660
Size: 94 700 2866
Fragments arranged by size:
2866 700 94
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2092 2962 3660
Size: 2092 870 698
Fragments arranged by size:
2092 870 698
MscI TGG'CCA
Cuts at: 0 1804 2456 3179 3660
Size: 1804 652 723 481
Fragments arranged by size:
1804 723 652 481
MslI CAynn'nnrTG
Cuts at: 0 668 912 1891 2540 3660
Size: 668 244 979 649 1120
Fragments arranged by size:
1120 979 668 649 244
MspA1I CmG'CkG
Cuts at: 0 515 961 2772 3660
Size: 515 446 1811 888
Fragments arranged by size:
1811 888 515 446
NcoI C'CATG_G
Cuts at: 0 2207 2499 3189 3660
Size: 2207 292 690 471
Fragments arranged by size:
2207 690 471 292
NdeI CA'TA_TG
Cuts at: 0 445 2588 3660
Size: 445 2143 1072
Fragments arranged by size:
2143 1072 445
NsiI A_TGCA'T
Cuts at: 0 644 2537 3566 3660
Size: 644 1893 1029 94
Fragments arranged by size:
1893 1029 644 94
NspI r_CATG'y
Cuts at: 0 2066 2111 3225 3295 3660
Size: 2066 45 1114 70 365
Fragments arranged by size:
2066 1114 365 70 45
PasI CC'CwG_GG
Cuts at: 0 3262 3660
Size: 3262 398
PflMI CCAn_nnn'nTGG
Cuts at: 0 584 2452 3660
Size: 584 1868 1208
Fragments arranged by size:
1868 1208 584
PfoI T'CCnGG_A
Cuts at: 0 764 2910 3660
Size: 764 2146 750
Fragments arranged by size:
2146 764 750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 2955 2987 3660
Size: 72 32 2851 32 673
Fragments arranged by size:
2851 673 72 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2968 3660
Size: 2968 692
PsiI TTA'TAA
Cuts at: 0 230 3660
Size: 230 3430
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2907 2939 3660
Size: 2907 32 721
Fragments arranged by size:
2907 721 32
PstI C_TGCA'G
Cuts at: 0 1893 1965 2054 2295 3110 3660
Size: 1893 72 89 241 815 550
Fragments arranged by size:
1893 815 550 241 89 72
PvuII CAG'CTG
Cuts at: 0 515 961 2772 3660
Size: 515 446 1811 888
Fragments arranged by size:
1811 888 515 446
SacI G_AGCT'C
Cuts at: 0 2880 3660
Size: 2880 780
SapI GCTCTTCn'nnn_
Cuts at: 0 1601 3660
Size: 1601 2059
ScaI AGT'ACT
Cuts at: 0 2220 3660
Size: 2220 1440
SfcI C'TryA_G
Cuts at: 0 1889 1961 2050 2222 2291 2378 3106 3660
Size: 1889 72 89 172 69 87 728 554
Fragments arranged by size:
1889 728 554 172 89 87 72 69
SmlI C'TyrA_G
Cuts at: 0 127 2057 2123 2201 3660
Size: 127 1930 66 78 1459
Fragments arranged by size:
1930 1459 127 78 66
SpeI A'CTAG_T
Cuts at: 0 2215 3660
Size: 2215 1445
SphI G_CATG'C
Cuts at: 0 3225 3660
Size: 3225 435
SspI AAT'ATT
Cuts at: 0 291 2491 3249 3660
Size: 291 2200 758 411
Fragments arranged by size:
2200 758 411 291
StuI AGG'CCT
Cuts at: 0 1176 2439 2794 3660
Size: 1176 1263 355 866
Fragments arranged by size:
1263 1176 866 355
StyI C'CwwG_G
Cuts at: 0 161 380 2207 2297 2429 2499 3189 3660
Size: 161 219 1827 90 132 70 690 471
Fragments arranged by size:
1827 690 471 219 161 132 90 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2611 3073 3643 3652 3660
Size: 2611 462 570 9 8
Fragments arranged by size:
2611 570 462 9 8
TatI w'GTAC_w
Cuts at: 0 2218 3660
Size: 2218 1442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 1529 1561 3660
Size: 746 32 751 32 2099
Fragments arranged by size:
2099 751 746 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 786 3187 3444 3660
Size: 786 2401 257 216
Fragments arranged by size:
2401 786 257 216
XmnI GAAnn'nnTTC
Cuts at: 0 394 1236 3660
Size: 394 842 2424
Fragments arranged by size:
2424 842 394
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AgeI AhdI AleI
AlfI AlwNI ApoI BaeI BanI BanII BbsI BbvCI
BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PflMI PfoI PpiI PpuMI PsiI PsrI
PstI PvuII SacI SapI ScaI SfcI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI AflII AflIII AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BamHI BbeI BcgI BclI
BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsmBI BspEI
BsrBI BsrGI BssHII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EcoRI FseI FspI FspAI HpaI
KasI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PshAI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I XbaI
XhoI XmaI ZraI