Restriction map for
pF1KA0909
(Linear) (Six-base) MAPSORT of: KIAA0909.seq Check: 4654 from: 1 to: 3606
KIAA0909.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1291 1425 1782 1968 3606
Size: 1291 134 357 186 1638
Fragments arranged by size:
1638 1291 357 186 134
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 340 541 574 1485 2732 3026 3220
Size: 67 273 201 33 911 1247 294 194
Cuts at: 3220 3577 3606
Size: 357 29
Fragments arranged by size:
1247 911 357 294 273 201 194 67
33 29
AfeI AGC'GCT
Cuts at: 0 3238 3606
Size: 3238 368
AflII C'TTAA_G
Cuts at: 0 3277 3606
Size: 3277 329
AhdI GACnn_n'nnGTC
Cuts at: 0 1887 3606
Size: 1887 1719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2168 3606
Size: 2168 1438
AlwNI CAG_nnn'CTG
Cuts at: 0 1972 2346 2500 2614 2923 3226 3573 3606
Size: 1972 374 154 114 309 303 347 33
Fragments arranged by size:
1972 374 347 309 303 154 114 33
ApaI G_GGCC'C
Cuts at: 0 1814 2717 3606
Size: 1814 903 889
Fragments arranged by size:
1814 903 889
ApaLI G'TGCA_C
Cuts at: 0 430 3606
Size: 430 3176
AvaI C'yCGr_G
Cuts at: 0 299 530 767 941 1201 1274 2396 2887
Size: 299 231 237 174 260 73 1122 491
Cuts at: 2887 3155 3606
Size: 268 451
Fragments arranged by size:
1122 491 451 299 268 260 237 231
174 73
AvrII C'CTAG_G
Cuts at: 0 1048 3606
Size: 1048 2558
BanI G'GyrC_C
Cuts at: 0 1980 2542 3606
Size: 1980 562 1064
Fragments arranged by size:
1980 1064 562
BanII G_rGCy'C
Cuts at: 0 767 848 911 1262 1358 1499 1814 1848
Size: 767 81 63 351 96 141 315 34
Cuts at: 1848 2462 2717 2933 3606
Size: 614 255 216 673
Fragments arranged by size:
767 673 614 351 315 255 216 141
96 81 63 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 298 3606
Size: 73 225 3308
Fragments arranged by size:
3308 225 73
BclI T'GATC_A
Cuts at: 0 152 1704 3606
Size: 152 1552 1902
Fragments arranged by size:
1902 1552 152
BglI GCCn_nnn'nGGC
Cuts at: 0 1023 3606
Size: 1023 2583
BglII A'GATC_T
Cuts at: 0 50 3606
Size: 50 3556
Bme1580I G_kGCm'C
Cuts at: 0 192 434 1814 2295 2577 2717 3606
Size: 192 242 1380 481 282 140 889
Fragments arranged by size:
1380 889 481 282 242 192 140
BmgBI CAC'GTC
Cuts at: 0 2558 3606
Size: 2558 1048
BmrI ACTGGGnnnn_n'
Cuts at: 0 1106 3606
Size: 1106 2500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1707 3084 3606
Size: 1707 1377 522
Fragments arranged by size:
1707 1377 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 94 391 475 1012 1025 1276 1513
Size: 89 5 297 84 537 13 251 237
Cuts at: 1513 1954 1991 2472 2750 2915 2986 3606
Size: 441 37 481 278 165 71 620
Fragments arranged by size:
620 537 481 441 297 278 251 237
165 89 84 71 37 13 5
Bpu10I CC'TnA_GC
Cuts at: 0 2188 3606
Size: 2188 1418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 2446 3606
Size: 1342 1104 1160
Fragments arranged by size:
1342 1160 1104
BsaI GGTCTCn'nnnn_
Cuts at: 0 2176 2932 3606
Size: 2176 756 674
Fragments arranged by size:
2176 756 674
BsaHI Gr'CG_yC
Cuts at: 0 1015 3606
Size: 1015 2591
BsaWI w'CCGG_w
Cuts at: 0 1910 2850 3245 3437 3606
Size: 1910 940 395 192 169
Fragments arranged by size:
1910 940 395 192 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 1460 1490 3391 3421 3457 3487
Size: 526 30 904 30 1901 30 36 30
Cuts at: 3487 3606
Size: 119
Fragments arranged by size:
1901 904 526 119 36 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 550 806 879 967 974 1097 1366
Size: 148 402 256 73 88 7 123 269
Cuts at: 1366 1466 1994 2475 2508 2671 2712 3606
Size: 100 528 481 33 163 41 894
Fragments arranged by size:
894 528 481 402 269 256 163 148
123 100 88 73 41 33 7
BseYI C'CCAG_C
Cuts at: 0 447 1577 1972 2986 3001 3587 3606
Size: 447 1130 395 1014 15 586 19
Fragments arranged by size:
1130 1014 586 447 395 19 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 2128 2582 3606
Size: 646 1482 454 1024
Fragments arranged by size:
1482 1024 646 454
BsiEI CG_ry'CG
Cuts at: 0 507 3606
Size: 507 3099
BsiHKAI G_wGCw'C
Cuts at: 0 434 848 1358 1655 1832 2321 3389 3606
Size: 434 414 510 297 177 489 1068 217
Fragments arranged by size:
1068 510 489 434 414 297 217 177
BsmI GAATG_Cn'
Cuts at: 0 2351 3606
Size: 2351 1255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2520 3606
Size: 2520 1086
Bsp1286I G_dGCh'C
Cuts at: 0 192 434 767 848 911 1262 1358 1499
Size: 192 242 333 81 63 351 96 141
Cuts at: 1499 1655 1814 1832 1848 2295 2321 2462 2577
Size: 156 159 18 16 447 26 141 115
Cuts at: 2577 2717 2933 3389 3606
Size: 140 216 456 217
Fragments arranged by size:
456 447 351 333 242 217 216 192
159 156 141 141 140 115 96 81
63 26 18 16
BspEI T'CCGG_A
Cuts at: 0 1910 3606
Size: 1910 1696
BspHI T'CATG_A
Cuts at: 0 3515 3606
Size: 3515 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1291 1425 1782 1968 3606
Size: 1291 134 357 186 1638
Fragments arranged by size:
1638 1291 357 186 134
BsrBI CCG'CTC
Cuts at: 0 1216 2247 2856 2913 3379 3407 3606
Size: 1216 1031 609 57 466 28 199
Fragments arranged by size:
1216 1031 609 466 199 57 28
BsrFI r'CCGG_y
Cuts at: 0 3191 3606
Size: 3191 415
BssSI C'ACGA_G
Cuts at: 0 3127 3606
Size: 3127 479
BstBI TT'CG_AA
Cuts at: 0 2039 3606
Size: 2039 1567
BstEII G'GTnAC_C
Cuts at: 0 262 3606
Size: 262 3344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1029 1065 1168 1491 2032 2964 3606
Size: 1029 36 103 323 541 932 642
Fragments arranged by size:
1029 932 642 541 323 103 36
BstYI r'GATC_y
Cuts at: 0 50 3606
Size: 50 3556
BtgI C'CryG_G
Cuts at: 0 2112 3606
Size: 2112 1494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1695 3606
Size: 1695 1911
BtsI GCAGTG_nn'
Cuts at: 0 693 965 1076 1721 3606
Size: 693 272 111 645 1885
Fragments arranged by size:
1885 693 645 272 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6 41 3606
Size: 6 35 3565
Fragments arranged by size:
3565 35 6
DraIII CAC_nnn'GTG
Cuts at: 0 624 3606
Size: 624 2982
EaeI y'GGCC_r
Cuts at: 0 2954 3311 3446 3599 3606
Size: 2954 357 135 153 7
Fragments arranged by size:
2954 357 153 135 7
EarI CTCTTCn'nnn_
Cuts at: 0 369 596 885 1305 1443 3606
Size: 369 227 289 420 138 2163
Fragments arranged by size:
2163 420 369 289 227 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2214 3606
Size: 2214 1392
EcoICRI GAG'CTC
Cuts at: 0 846 1356 3606
Size: 846 510 2250
Fragments arranged by size:
2250 846 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 89 94 340 391 475 541 574
Size: 67 22 5 246 51 84 66 33
Cuts at: 574 1012 1025 1276 1485 1513 1954 1991 2472
Size: 438 13 251 209 28 441 37 481
Cuts at: 2472 2732 2750 2915 2986 3026 3220 3577 3606
Size: 260 18 165 71 40 194 357 29
Fragments arranged by size:
481 441 438 357 260 251 246 209
194 165 84 71 67 66 51 40
37 33 29 28 22 18 13 5
EcoO109I rG'GnC_Cy
Cuts at: 0 1279 1471 1663 1810 1811 1993 2029 2095
Size: 1279 192 192 147 1 182 36 66
Cuts at: 2095 2713 2714 3606
Size: 618 1 892
Fragments arranged by size:
1279 892 618 192 192 182 147 66
36 1 1
HaeII r_GCGC'y
Cuts at: 0 393 3240 3534 3606
Size: 393 2847 294 72
Fragments arranged by size:
2847 393 294 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 166 189 198 221 1523 1555
Size: 127 32 7 23 9 23 1302 32
Cuts at: 1555 1873 1905 2248 2280 2803 2835 2929 2961
Size: 318 32 343 32 523 32 94 32
Cuts at: 2961 3606
Size: 645
Fragments arranged by size:
1302 645 523 343 318 127 94 32
32 32 32 32 32 23 23 9
7
HincII GTy'rAC
Cuts at: 0 2241 3606
Size: 2241 1365
HindIII A'AGCT_T
Cuts at: 0 2217 3606
Size: 2217 1389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 2311 2516 3606
Size: 1594 717 205 1090
Fragments arranged by size:
1594 1090 717 205
MscI TGG'CCA
Cuts at: 0 2956 3601 3606
Size: 2956 645 5
Fragments arranged by size:
2956 645 5
MslI CAynn'nnrTG
Cuts at: 0 1714 3606
Size: 1714 1892
MspA1I CmG'CkG
Cuts at: 0 96 552 999 1270 1581 1972 2420 3135
Size: 96 456 447 271 311 391 448 715
Cuts at: 3135 3226 3261 3606
Size: 91 35 345
Fragments arranged by size:
715 456 448 447 391 345 311 271
96 91 35
NaeI GCC'GGC
Cuts at: 0 3193 3606
Size: 3193 413
NcoI C'CATG_G
Cuts at: 0 2112 3606
Size: 2112 1494
NdeI CA'TA_TG
Cuts at: 0 1167 3606
Size: 1167 2439
NgoMIV G'CCGG_C
Cuts at: 0 3191 3606
Size: 3191 415
PasI CC'CwG_GG
Cuts at: 0 2160 2295 3606
Size: 2160 135 1311
Fragments arranged by size:
2160 1311 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 1639 2500 2948 3573 3606
Size: 333 1306 861 448 625 33
Fragments arranged by size:
1306 861 625 448 333 33
PpuMI rG'GwC_Cy
Cuts at: 0 1279 1471 1663 1993 2095 3606
Size: 1279 192 192 330 102 1511
Fragments arranged by size:
1511 1279 330 192 192 102
PshAI GACnn'nnGTC
Cuts at: 0 2501 3606
Size: 2501 1105
PspOMI G'GGCC_C
Cuts at: 0 1810 2713 3606
Size: 1810 903 893
Fragments arranged by size:
1810 903 893
PstI C_TGCA'G
Cuts at: 0 478 688 3606
Size: 478 210 2918
Fragments arranged by size:
2918 478 210
PvuII CAG'CTG
Cuts at: 0 552 1270 1581 1972 3226 3261 3606
Size: 552 718 311 391 1254 35 345
Fragments arranged by size:
1254 718 552 391 345 311 35
SacI G_AGCT'C
Cuts at: 0 848 1358 3606
Size: 848 510 2248
Fragments arranged by size:
2248 848 510
SanDI GG'GwC_CC
Cuts at: 0 1279 3606
Size: 1279 2327
ScaI AGT'ACT
Cuts at: 0 1872 3606
Size: 1872 1734
SexAI A'CCwGG_T
Cuts at: 0 620 3606
Size: 620 2986
SfcI C'TryA_G
Cuts at: 0 474 611 684 2434 3606
Size: 474 137 73 1750 1172
Fragments arranged by size:
1750 1172 474 137 73
SmaI CCC'GGG
Cuts at: 0 532 2398 3606
Size: 532 1866 1208
Fragments arranged by size:
1866 1208 532
SmlI C'TyrA_G
Cuts at: 0 941 1357 2425 3277 3606
Size: 941 416 1068 852 329
Fragments arranged by size:
1068 941 852 416 329
StuI AGG'CCT
Cuts at: 0 204 1053 1459 3606
Size: 204 849 406 2147
Fragments arranged by size:
2147 849 406 204
StyI C'CwwG_G
Cuts at: 0 8 851 1048 1424 1666 2112 2708 3606
Size: 8 843 197 376 242 446 596 898
Fragments arranged by size:
898 843 596 446 376 242 197 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 662 1395 1687 3606
Size: 658 4 733 292 1919
Fragments arranged by size:
1919 733 658 292 4
TatI w'GTAC_w
Cuts at: 0 1870 3182 3251 3606
Size: 1870 1312 69 355
Fragments arranged by size:
1870 1312 355 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3391 3423 3606
Size: 3391 32 183
Fragments arranged by size:
3391 183 32
Tth111I GACn'n_nGTC
Cuts at: 0 421 2943 3606
Size: 421 2522 663
Fragments arranged by size:
2522 663 421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1026 2084 2653 2949 3087 3606
Size: 69 957 1058 569 296 138 519
Fragments arranged by size:
1058 957 569 519 296 138 69
XhoI C'TCGA_G
Cuts at: 0 941 3606
Size: 941 2665
XmaI C'CCGG_G
Cuts at: 0 530 2396 3606
Size: 530 1866 1210
Fragments arranged by size:
1866 1210 530
XmnI GAAnn'nnTTC
Cuts at: 0 3581 3606
Size: 3581 25
Enzymes that do cut:
AarI AcuI AfeI AflII AhdI AlfI AlwNI ApaI
ApaLI AvaI AvrII BanI BanII BbsI BclI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV PasI PflMI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI ScaI SexAI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AleI AloI
ApoI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BciVI BfrBI BlpI BmtI BsaAI BsaBI BsiWI
BsrDI BsrGI BssHII BstAPI BstZ17I Bsu36I ClaI DraI
DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI NarI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI ZraI