Restriction map for
pF1KA0910
(Linear) (Six-base) MAPSORT of: KIAA0910.seq Check: 6249 from: 1 to: 3837
KIAA0910.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 846 3837
Size: 846 2991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 314 1828 2483 2581 2645 3080 3323
Size: 299 15 1514 655 98 64 435 243
Cuts at: 3323 3646 3779 3837
Size: 323 133 58
Fragments arranged by size:
1514 655 435 323 299 243 133 98
64 58 15
AflII C'TTAA_G
Cuts at: 0 865 3837
Size: 865 2972
AflIII A'CryG_T
Cuts at: 0 2516 3837
Size: 2516 1321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2554 2780 2983 3837
Size: 2554 226 203 854
Fragments arranged by size:
2554 854 226 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 3837
Size: 1094 32 2711
Fragments arranged by size:
2711 1094 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1327 3466 3837
Size: 1327 2139 371
Fragments arranged by size:
2139 1327 371
ApaLI G'TGCA_C
Cuts at: 0 3239 3837
Size: 3239 598
ApoI r'AATT_y
Cuts at: 0 266 369 897 993 1566 1595 2507 2925
Size: 266 103 528 96 573 29 912 418
Cuts at: 2925 3373 3738 3837
Size: 448 365 99
Fragments arranged by size:
912 573 528 448 418 365 266 103
99 96 29
AvaI C'yCGr_G
Cuts at: 0 1478 3837
Size: 1478 2359
BamHI G'GATC_C
Cuts at: 0 39 3837
Size: 39 3798
BanII G_rGCy'C
Cuts at: 0 1335 2234 3837
Size: 1335 899 1603
Fragments arranged by size:
1603 1335 899
BbsI GAAGACnn'nnnn_
Cuts at: 0 1855 1981 2569 3561 3647 3837
Size: 1855 126 588 992 86 190
Fragments arranged by size:
1855 992 588 190 126 86
BbvCI CC'TCA_GC
Cuts at: 0 3080 3837
Size: 3080 757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1127 1161 3837
Size: 1127 34 2676
Fragments arranged by size:
2676 1127 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3466 3837
Size: 3466 371
BfrBI ATG'CAT
Cuts at: 0 175 3837
Size: 175 3662
BglII A'GATC_T
Cuts at: 0 2466 2472 3837
Size: 2466 6 1365
Fragments arranged by size:
2466 1365 6
BlpI GC'TnA_GC
Cuts at: 0 2555 3837
Size: 2555 1282
Bme1580I G_kGCm'C
Cuts at: 0 3243 3525 3837
Size: 3243 282 312
Fragments arranged by size:
3243 312 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 1450 3230 3837
Size: 1450 1780 607
Fragments arranged by size:
1780 1450 607
BmtI G_CTAG'C
Cuts at: 0 1207 3837
Size: 1207 2630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2853 3347 3837
Size: 2853 494 490
Fragments arranged by size:
2853 494 490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1842 2510 3073 3530 3837
Size: 1349 493 668 563 457 307
Fragments arranged by size:
1349 668 563 493 457 307
Bpu10I CC'TnA_GC
Cuts at: 0 3080 3837
Size: 3080 757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 3425 3837
Size: 1043 2382 412
Fragments arranged by size:
2382 1043 412
BsaI GGTCTCn'nnnn_
Cuts at: 0 2697 3318 3837
Size: 2697 621 519
Fragments arranged by size:
2697 621 519
BsaAI yAC'GTr
Cuts at: 0 2519 3837
Size: 2519 1318
BsaBI GATnn'nnATC
Cuts at: 0 338 2471 3837
Size: 338 2133 1366
Fragments arranged by size:
2133 1366 338
BsaHI Gr'CG_yC
Cuts at: 0 1904 3837
Size: 1904 1933
BsaWI w'CCGG_w
Cuts at: 0 20 3837
Size: 20 3817
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2325 2355 2508 2538 3483 3513 3837
Size: 2325 30 153 30 945 30 324
Fragments arranged by size:
2325 945 324 153 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 569 3320 3491 3837
Size: 569 2751 171 346
Fragments arranged by size:
2751 569 346 171
BseYI C'CCAG_C
Cuts at: 0 634 3124 3731 3837
Size: 634 2490 607 106
Fragments arranged by size:
2490 634 607 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 2524 3341 3837
Size: 1198 1326 817 496
Fragments arranged by size:
1326 1198 817 496
BsiEI CG_ry'CG
Cuts at: 0 3293 3837
Size: 3293 544
BsiHKAI G_wGCw'C
Cuts at: 0 122 1335 1368 1485 2234 3213 3243 3384
Size: 122 1213 33 117 749 979 30 141
Cuts at: 3384 3837
Size: 453
Fragments arranged by size:
1213 979 749 453 141 122 117 33
30
BsmI GAATG_Cn'
Cuts at: 0 1791 2030 3837
Size: 1791 239 1807
Fragments arranged by size:
1807 1791 239
Bsp1286I G_dGCh'C
Cuts at: 0 122 1335 1368 1485 2234 3213 3243 3384
Size: 122 1213 33 117 749 979 30 141
Cuts at: 3384 3525 3837
Size: 141 312
Fragments arranged by size:
1213 979 749 312 141 141 122 117
33 30
BspEI T'CCGG_A
Cuts at: 0 20 3837
Size: 20 3817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 846 3474 3764 3837
Size: 846 2628 290 73
Fragments arranged by size:
2628 846 290 73
BsrDI GCAATG_nn'
Cuts at: 0 2960 2965 3837
Size: 2960 5 872
Fragments arranged by size:
2960 872 5
BsrGI T'GTAC_A
Cuts at: 0 2513 3837
Size: 2513 1324
BssSI C'ACGA_G
Cuts at: 0 3503 3837
Size: 3503 334
BstAPI GCAn_nnn'nTGC
Cuts at: 0 397 3013 3451 3837
Size: 397 2616 438 386
Fragments arranged by size:
2616 438 397 386
BstBI TT'CG_AA
Cuts at: 0 1137 3837
Size: 1137 2700
BstEII G'GTnAC_C
Cuts at: 0 2331 3837
Size: 2331 1506
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 767 3837
Size: 168 599 3070
Fragments arranged by size:
3070 599 168
BstYI r'GATC_y
Cuts at: 0 23 39 751 793 907 2466 2472 3837
Size: 23 16 712 42 114 1559 6 1365
Fragments arranged by size:
1559 1365 712 114 42 23 16 6
Bsu36I CC'TnA_GG
Cuts at: 0 3632 3837
Size: 3632 205
BtsI GCAGTG_nn'
Cuts at: 0 313 3837
Size: 313 3524
ClaI AT'CG_AT
Cuts at: 0 323 2204 3837
Size: 323 1881 1633
Fragments arranged by size:
1881 1633 323
DraI TTT'AAA
Cuts at: 0 2903 3837
Size: 2903 934
DrdI GACnn_nn'nnGTC
Cuts at: 0 1527 3837
Size: 1527 2310
EarI CTCTTCn'nnn_
Cuts at: 0 2035 2544 2621 3135 3837
Size: 2035 509 77 514 702
Fragments arranged by size:
2035 702 514 509 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3323 3837
Size: 3323 514
EcoICRI GAG'CTC
Cuts at: 0 1333 2232 3837
Size: 1333 899 1605
Fragments arranged by size:
1605 1333 899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 314 1349 1828 1842 2483 2510 2581
Size: 299 15 1035 479 14 641 27 71
Cuts at: 2581 2645 3073 3080 3323 3530 3646 3779 3837
Size: 64 428 7 243 207 116 133 58
Fragments arranged by size:
1035 641 479 428 299 243 207 133
116 71 64 58 27 15 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3443 3837
Size: 3443 394
EcoO109I rG'GnC_Cy
Cuts at: 0 3175 3463 3837
Size: 3175 288 374
Fragments arranged by size:
3175 374 288
EcoRI G'AATT_C
Cuts at: 0 369 3837
Size: 369 3468
EcoRV GAT'ATC
Cuts at: 0 981 3837
Size: 981 2856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 956 2352 2376 2803 3466 3709 3837
Size: 956 1396 24 427 663 243 128
Fragments arranged by size:
1396 956 663 427 243 128 24
FspI TGC'GCA
Cuts at: 0 434 3837
Size: 434 3403
FspAI rTGC'GCAy
Cuts at: 0 434 3837
Size: 434 3403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 295 327 548 580 1592 1624
Size: 8 32 255 32 221 32 1012 32
Cuts at: 1624 3483 3486 3515 3518 3814 3837
Size: 1859 3 29 3 296 23
Fragments arranged by size:
1859 1012 296 255 221 32 32 32
32 29 23 8 3 3
HindIII A'AGCT_T
Cuts at: 0 938 959 3800 3837
Size: 938 21 2841 37
Fragments arranged by size:
2841 938 37 21
MfeI C'AATT_G
Cuts at: 0 1757 3837
Size: 1757 2080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1196 1590 2368 3837
Size: 240 956 394 778 1469
Fragments arranged by size:
1469 956 778 394 240
MslI CAynn'nnrTG
Cuts at: 0 2168 3837
Size: 2168 1669
MspA1I CmG'CkG
Cuts at: 0 2461 3837
Size: 2461 1376
NdeI CA'TA_TG
Cuts at: 0 177 926 3837
Size: 177 749 2911
Fragments arranged by size:
2911 749 177
NheI G'CTAG_C
Cuts at: 0 1203 3837
Size: 1203 2634
NsiI A_TGCA'T
Cuts at: 0 177 3837
Size: 177 3660
NspI r_CATG'y
Cuts at: 0 447 973 1560 1939 3837
Size: 447 526 587 379 1898
Fragments arranged by size:
1898 587 526 447 379
PflMI CCAn_nnn'nTGG
Cuts at: 0 2677 3837
Size: 2677 1160
PfoI T'CCnGG_A
Cuts at: 0 1712 3837
Size: 1712 2125
PmlI CAC'GTG
Cuts at: 0 2519 3837
Size: 2519 1318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 740 772 1317 1349 3837
Size: 740 32 545 32 2488
Fragments arranged by size:
2488 740 545 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3175 3463 3837
Size: 3175 288 374
Fragments arranged by size:
3175 374 288
PsiI TTA'TAA
Cuts at: 0 2645 2791 3837
Size: 2645 146 1046
Fragments arranged by size:
2645 1046 146
PspXI vC'TCGA_Gb
Cuts at: 0 1478 3837
Size: 1478 2359
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2645 2677 3837
Size: 2645 32 1160
Fragments arranged by size:
2645 1160 32
PstI C_TGCA'G
Cuts at: 0 132 2076 2759 3837
Size: 132 1944 683 1078
Fragments arranged by size:
1944 1078 683 132
PvuII CAG'CTG
Cuts at: 0 2461 3837
Size: 2461 1376
SacI G_AGCT'C
Cuts at: 0 1335 2234 3837
Size: 1335 899 1603
Fragments arranged by size:
1603 1335 899
SapI GCTCTTCn'nnn_
Cuts at: 0 2035 2544 3135 3837
Size: 2035 509 591 702
Fragments arranged by size:
2035 702 591 509
ScaI AGT'ACT
Cuts at: 0 167 1547 3837
Size: 167 1380 2290
Fragments arranged by size:
2290 1380 167
SexAI A'CCwGG_T
Cuts at: 0 640 3837
Size: 640 3197
SfcI C'TryA_G
Cuts at: 0 128 1835 2058 2072 2755 3051 3837
Size: 128 1707 223 14 683 296 786
Fragments arranged by size:
1707 786 683 296 223 128 14
SmlI C'TyrA_G
Cuts at: 0 865 1058 1478 3440 3837
Size: 865 193 420 1962 397
Fragments arranged by size:
1962 865 420 397 193
SpeI A'CTAG_T
Cuts at: 0 3142 3837
Size: 3142 695
SphI G_CATG'C
Cuts at: 0 447 1939 3837
Size: 447 1492 1898
Fragments arranged by size:
1898 1492 447
SspI AAT'ATT
Cuts at: 0 1579 3837
Size: 1579 2258
StuI AGG'CCT
Cuts at: 0 2580 3837
Size: 2580 1257
StyI C'CwwG_G
Cuts at: 0 3111 3837
Size: 3111 726
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1265 1710 3375 3399 3504 3837
Size: 1265 445 1665 24 105 333
Fragments arranged by size:
1665 1265 445 333 105 24
TatI w'GTAC_w
Cuts at: 0 165 710 1545 2513 3478 3837
Size: 165 545 835 968 965 359
Fragments arranged by size:
968 965 835 545 359 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1784 1816 2403 2435 2508 2540 3837
Size: 1784 32 587 32 73 32 1297
Fragments arranged by size:
1784 1297 587 73 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2419 3425 3837
Size: 2419 1006 412
Fragments arranged by size:
2419 1006 412
XbaI T'CTAG_A
Cuts at: 0 1074 2469 3837
Size: 1074 1395 1368
Fragments arranged by size:
1395 1368 1074
XhoI C'TCGA_G
Cuts at: 0 1478 3837
Size: 1478 2359
XmnI GAAnn'nnTTC
Cuts at: 0 324 1271 1396 3074 3837
Size: 324 947 125 1678 763
Fragments arranged by size:
1678 947 763 324 125
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AloI AlwNI ApaLI
ApoI AvaI BamHI BanII BbsI BbvCI BcgI BciVI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtsI ClaI DraI DrdI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI Hin4I HindIII MfeI MmeI MslI MspA1I NdeI
NheI NsiI NspI PflMI PfoI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI
BclI BglI BmgBI BsiWI BsmBI BspHI BsrBI BsrFI
BssHII BstZ17I BtgI BtgZI CspCI DraIII EaeI EagI
FseI HaeII HincII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NgoMIV NotI NruI PacI PasI
PciI PmeI PshAI PspOMI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SwaI XcmI XmaI ZraI