Restriction map for
pF1KA0911
(Linear) (Six-base) MAPSORT of: KIAA0911.seq Check: 9294 from: 1 to: 2943
KIAA0911.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 787 1564 1909 2327 2943
Size: 787 777 345 418 616
Fragments arranged by size:
787 777 616 418 345
AccI GT'mk_AC
Cuts at: 0 1675 2943
Size: 1675 1268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2102 2943
Size: 2102 841
AflIII A'CryG_T
Cuts at: 0 871 2943
Size: 871 2072
AleI CACnn'nnGTG
Cuts at: 0 421 2281 2768 2943
Size: 421 1860 487 175
Fragments arranged by size:
1860 487 421 175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1744 2943
Size: 1744 1199
AlwNI CAG_nnn'CTG
Cuts at: 0 2002 2913 2943
Size: 2002 911 30
Fragments arranged by size:
2002 911 30
ApaLI G'TGCA_C
Cuts at: 0 2140 2494 2943
Size: 2140 354 449
Fragments arranged by size:
2140 449 354
ApoI r'AATT_y
Cuts at: 0 263 1396 1850 2005 2413 2943
Size: 263 1133 454 155 408 530
Fragments arranged by size:
1133 530 454 408 263 155
AvaI C'yCGr_G
Cuts at: 0 1020 1401 1783 1948 1991 2943
Size: 1020 381 382 165 43 952
Fragments arranged by size:
1020 952 382 381 165 43
BamHI G'GATC_C
Cuts at: 0 1027 1115 1299 2642 2943
Size: 1027 88 184 1343 301
Fragments arranged by size:
1343 1027 301 184 88
BanI G'GyrC_C
Cuts at: 0 415 829 1099 1244 1271 1373 2943
Size: 415 414 270 145 27 102 1570
Fragments arranged by size:
1570 415 414 270 145 102 27
BanII G_rGCy'C
Cuts at: 0 119 1442 1958 2943
Size: 119 1323 516 985
Fragments arranged by size:
1323 985 516 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 1718 1784 2943
Size: 1718 66 1159
Fragments arranged by size:
1718 1159 66
BbvCI CC'TCA_GC
Cuts at: 0 2096 2935 2943
Size: 2096 839 8
Fragments arranged by size:
2096 839 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 2145 2943
Size: 2145 798
BglI GCCn_nnn'nGGC
Cuts at: 0 1973 2943
Size: 1973 970
Bme1580I G_kGCm'C
Cuts at: 0 629 832 1000 2144 2498 2943
Size: 629 203 168 1144 354 445
Fragments arranged by size:
1144 629 445 354 203 168
BmgBI CAC'GTC
Cuts at: 0 468 624 1430 2943
Size: 468 156 806 1513
Fragments arranged by size:
1513 806 468 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 1590 2272 2715 2943
Size: 1590 682 443 228
Fragments arranged by size:
1590 682 443 228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1427 1461 1798 2943
Size: 1427 34 337 1145
Fragments arranged by size:
1427 1145 337 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 602 886 2227 2935 2943
Size: 133 469 284 1341 708 8
Fragments arranged by size:
1341 708 469 284 133 8
Bpu10I CC'TnA_GC
Cuts at: 0 2039 2096 2935 2943
Size: 2039 57 839 8
Fragments arranged by size:
2039 839 57 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 2740 2943
Size: 2740 203
BsaAI yAC'GTr
Cuts at: 0 1932 2943
Size: 1932 1011
BsaBI GATnn'nnATC
Cuts at: 0 1238 2943
Size: 1238 1705
BsaHI Gr'CG_yC
Cuts at: 0 1127 1976 2943
Size: 1127 849 967
Fragments arranged by size:
1127 967 849
BsaWI w'CCGG_w
Cuts at: 0 2360 2943
Size: 2360 583
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1237 1267 1810 1840 2943
Size: 1237 30 543 30 1103
Fragments arranged by size:
1237 1103 543 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 1324 1966 2146 2194 2320 2788 2791
Size: 151 1173 642 180 48 126 468 3
Cuts at: 2791 2794 2875 2878 2881 2943
Size: 3 81 3 3 62
Fragments arranged by size:
1173 642 468 180 151 126 81 62
48 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1747 2480 2569 2943
Size: 1747 733 89 374
Fragments arranged by size:
1747 733 374 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1570 1750 2943
Size: 765 805 180 1193
Fragments arranged by size:
1193 805 765 180
BsiEI CG_ry'CG
Cuts at: 0 35 945 992 2943
Size: 35 910 47 1951
Fragments arranged by size:
1951 910 47 35
BsiHKAI G_wGCw'C
Cuts at: 0 161 2144 2498 2943
Size: 161 1983 354 445
Fragments arranged by size:
1983 445 354 161
Bsp1286I G_dGCh'C
Cuts at: 0 119 161 629 832 1000 1442 1958 2144
Size: 119 42 468 203 168 442 516 186
Cuts at: 2144 2498 2943
Size: 354 445
Fragments arranged by size:
516 468 445 442 354 203 186 168
119 42
BspHI T'CATG_A
Cuts at: 0 2618 2943
Size: 2618 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 787 1335 1564 1592 1687 1692 1706 1909
Size: 787 548 229 28 95 5 14 203
Cuts at: 1909 2327 2943
Size: 418 616
Fragments arranged by size:
787 616 548 418 229 203 95 28
14 5
BsrBI CCG'CTC
Cuts at: 0 16 1467 2510 2943
Size: 16 1451 1043 433
Fragments arranged by size:
1451 1043 433 16
BsrFI r'CCGG_y
Cuts at: 0 832 974 1274 2943
Size: 832 142 300 1669
Fragments arranged by size:
1669 832 300 142
BssSI C'ACGA_G
Cuts at: 0 1435 1495 2064 2191 2943
Size: 1435 60 569 127 752
Fragments arranged by size:
1435 752 569 127 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2322 2943
Size: 2322 621
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1755 2454 2472 2943
Size: 1755 699 18 471
Fragments arranged by size:
1755 699 471 18
BstYI r'GATC_y
Cuts at: 0 1027 1115 1299 1733 2642 2943
Size: 1027 88 184 434 909 301
Fragments arranged by size:
1027 909 434 301 184 88
BstZ17I GTA'TAC
Cuts at: 0 1676 2943
Size: 1676 1267
BtgI C'CryG_G
Cuts at: 0 126 512 560 992 1043 1266 2291 2453
Size: 126 386 48 432 51 223 1025 162
Cuts at: 2453 2741 2943
Size: 288 202
Fragments arranged by size:
1025 432 386 288 223 202 162 126
51 48
BtsI GCAGTG_nn'
Cuts at: 0 299 710 998 1115 2573 2775 2943
Size: 299 411 288 117 1458 202 168
Fragments arranged by size:
1458 411 299 288 202 168 117
DraI TTT'AAA
Cuts at: 0 261 2943
Size: 261 2682
DrdI GACnn_nn'nnGTC
Cuts at: 0 883 1702 2943
Size: 883 819 1241
Fragments arranged by size:
1241 883 819
EaeI y'GGCC_r
Cuts at: 0 32 50 842 989 1065 2294 2394 2444
Size: 32 18 792 147 76 1229 100 50
Cuts at: 2444 2540 2943
Size: 96 403
Fragments arranged by size:
1229 792 403 147 100 96 76 50
32 18
EagI C'GGCC_G
Cuts at: 0 32 989 2943
Size: 32 957 1954
Fragments arranged by size:
1954 957 32
EarI CTCTTCn'nnn_
Cuts at: 0 1294 2780 2786 2792 2816 2943
Size: 1294 1486 6 6 24 127
Fragments arranged by size:
1486 1294 127 24 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2676 2943
Size: 2676 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 602 886 2102 2227 2935 2943
Size: 133 469 284 1216 125 708 8
Fragments arranged by size:
1216 708 469 284 133 125 8
EcoO109I rG'GnC_Cy
Cuts at: 0 406 1704 2100 2348 2943
Size: 406 1298 396 248 595
Fragments arranged by size:
1298 595 406 396 248
EcoRI G'AATT_C
Cuts at: 0 1396 1850 2943
Size: 1396 454 1093
Fragments arranged by size:
1396 1093 454
HaeII r_GCGC'y
Cuts at: 0 2843 2943
Size: 2843 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 1414 1446 1473 1505 1653 1685
Size: 940 32 442 32 27 32 148 32
Cuts at: 1685 1850 1882 2943
Size: 165 32 1061
Fragments arranged by size:
1061 940 442 165 148 32 32 32
32 32 27
HincII GTy'rAC
Cuts at: 0 93 639 2520 2736 2943
Size: 93 546 1881 216 207
Fragments arranged by size:
1881 546 216 207 93
HpaI GTT'AAC
Cuts at: 0 93 2943
Size: 93 2850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2828 2943
Size: 2828 115
MscI TGG'CCA
Cuts at: 0 1067 2296 2542 2943
Size: 1067 1229 246 401
Fragments arranged by size:
1229 1067 401 246
MslI CAynn'nnrTG
Cuts at: 0 421 1165 2245 2281 2768 2943
Size: 421 744 1080 36 487 175
Fragments arranged by size:
1080 744 487 421 175 36
MspA1I CmG'CkG
Cuts at: 0 994 2913 2943
Size: 994 1919 30
Fragments arranged by size:
1919 994 30
NaeI GCC'GGC
Cuts at: 0 1276 2943
Size: 1276 1667
NcoI C'CATG_G
Cuts at: 0 1043 2291 2453 2741 2943
Size: 1043 1248 162 288 202
Fragments arranged by size:
1248 1043 288 202 162
NgoMIV G'CCGG_C
Cuts at: 0 1274 2943
Size: 1274 1669
NotI GC'GGCC_GC
Cuts at: 0 989 2943
Size: 989 1954
NspI r_CATG'y
Cuts at: 0 875 2344 2943
Size: 875 1469 599
Fragments arranged by size:
1469 875 599
PasI CC'CwG_GG
Cuts at: 0 787 2943
Size: 787 2156
PciI A'CATG_T
Cuts at: 0 871 2943
Size: 871 2072
PflMI CCAn_nnn'nTGG
Cuts at: 0 2538 2943
Size: 2538 405
PfoI T'CCnGG_A
Cuts at: 0 1118 1636 2943
Size: 1118 518 1307
Fragments arranged by size:
1307 1118 518
PpuMI rG'GwC_Cy
Cuts at: 0 406 1704 2100 2348 2943
Size: 406 1298 396 248 595
Fragments arranged by size:
1298 595 406 396 248
PstI C_TGCA'G
Cuts at: 0 362 1332 1575 1703 2231 2943
Size: 362 970 243 128 528 712
Fragments arranged by size:
970 712 528 362 243 128
PvuII CAG'CTG
Cuts at: 0 2913 2943
Size: 2913 30
SacII CC_GC'GG
Cuts at: 0 995 2943
Size: 995 1948
SbfI CC_TGCA'GG
Cuts at: 0 1703 2943
Size: 1703 1240
SexAI A'CCwGG_T
Cuts at: 0 1079 2943
Size: 1079 1864
SfcI C'TryA_G
Cuts at: 0 358 1328 1571 1699 2227 2943
Size: 358 970 243 128 528 716
Fragments arranged by size:
970 716 528 358 243 128
SgrAI Cr'CCGG_yG
Cuts at: 0 832 2943
Size: 832 2111
SphI G_CATG'C
Cuts at: 0 2344 2943
Size: 2344 599
StuI AGG'CCT
Cuts at: 0 388 2943
Size: 388 2555
StyI C'CwwG_G
Cuts at: 0 1043 1766 2291 2453 2741 2943
Size: 1043 723 525 162 288 202
Fragments arranged by size:
1043 723 525 288 202 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 779 959 1117 1125 1899 2943
Size: 779 180 158 8 774 1044
Fragments arranged by size:
1044 779 774 180 158 8
TatI w'GTAC_w
Cuts at: 0 894 2943
Size: 894 2049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2943
Size: 1237 32 1674
Fragments arranged by size:
1674 1237 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 979 2943
Size: 342 637 1964
Fragments arranged by size:
1964 637 342
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstAPI BstXI BstYI BstZ17I BtgI BtsI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PpuMI PstI PvuII SacII SbfI SexAI SfcI SgrAI
SphI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BclI BfrBI BglII BlpI BmtI BpuEI BsiWI BsmI
BsmBI BspEI BsrDI BsrGI BssHII BstBI BstEII Bsu36I
BtgZI ClaI CspCI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NarI NdeI NheI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI ScaI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI