(Linear) (Six-base) MAPSORT of: KIAA0913.seq Check: 8447 from: 1 to: 5388 KIAA0913.orf With 182 enzymes: SgfI * August 18, 2008 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 237 1786 4174 4234 4451 4874 5138 5388 Size: 237 1549 2388 60 217 423 264 250 Fragments arranged by size: 2388 1549 423 264 250 237 217 60 Acc65I G'GTAC_C Cuts at: 0 193 5388 Size: 193 5195 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 921 1378 1724 2203 4151 5388 Size: 921 457 346 479 1948 1237 Fragments arranged by size: 1948 1237 921 479 457 346 AfeI AGC'GCT Cuts at: 0 37 5164 5388 Size: 37 5127 224 Fragments arranged by size: 5127 224 37 AflII C'TTAA_G Cuts at: 0 2911 3375 5388 Size: 2911 464 2013 Fragments arranged by size: 2911 2013 464 AflIII A'CryG_T Cuts at: 0 2845 5388 Size: 2845 2543 AhdI GACnn_n'nnGTC Cuts at: 0 4832 5388 Size: 4832 556 AleI CACnn'nnGTG Cuts at: 0 467 1187 4342 4621 4702 5132 5388 Size: 467 720 3155 279 81 430 256 Fragments arranged by size: 3155 720 467 430 279 256 81 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 719 5388 Size: 719 4669 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2347 2379 5388 Size: 2347 32 3009 Fragments arranged by size: 3009 2347 32 AlwNI CAG_nnn'CTG Cuts at: 0 301 985 1954 2059 2298 2785 2970 3079 Size: 301 684 969 105 239 487 185 109 Cuts at: 3079 3088 3115 4294 5103 5388 Size: 9 27 1179 809 285 Fragments arranged by size: 1179 969 809 684 487 301 285 239 185 109 105 27 9 ApaI G_GGCC'C Cuts at: 0 152 836 1527 1533 3040 3057 3408 4041 Size: 152 684 691 6 1507 17 351 633 Cuts at: 4041 4288 5388 Size: 247 1100 Fragments arranged by size: 1507 1100 691 684 633 351 247 152 17 6 ApaLI G'TGCA_C Cuts at: 0 5020 5388 Size: 5020 368 AvaI C'yCGr_G Cuts at: 0 1526 1580 3521 3565 4377 4816 5365 5388 Size: 1526 54 1941 44 812 439 549 23 Fragments arranged by size: 1941 1526 812 549 439 54 44 23 AvrII C'CTAG_G Cuts at: 0 4569 5388 Size: 4569 819 BanI G'GyrC_C Cuts at: 0 193 480 1319 1549 2609 3896 4344 4647 Size: 193 287 839 230 1060 1287 448 303 Cuts at: 4647 4753 5388 Size: 106 635 Fragments arranged by size: 1287 1060 839 635 448 303 287 230 193 106 BanII G_rGCy'C Cuts at: 0 152 654 728 816 836 1527 1533 1792 Size: 152 502 74 88 20 691 6 259 Cuts at: 1792 1930 3040 3057 3408 3475 3522 4041 4288 Size: 138 1110 17 351 67 47 519 247 Cuts at: 4288 4578 5062 5388 Size: 290 484 326 Fragments arranged by size: 1110 691 519 502 484 351 326 290 259 247 152 138 88 74 67 47 20 17 6 BbeI G_GCGC'C Cuts at: 0 1323 5388 Size: 1323 4065 BbsI GAAGACnn'nnnn_ Cuts at: 0 770 927 956 1402 1835 2806 3181 3535 Size: 770 157 29 446 433 971 375 354 Cuts at: 3535 3643 4512 5388 Size: 108 869 876 Fragments arranged by size: 971 876 869 770 446 433 375 354 157 108 29 BbvCI CC'TCA_GC Cuts at: 0 731 837 1173 2231 5388 Size: 731 106 336 1058 3157 Fragments arranged by size: 3157 1058 731 336 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 579 613 2155 2189 3556 3590 3624 3658 Size: 579 34 1542 34 1367 34 34 34 Cuts at: 3658 5388 Size: 1730 Fragments arranged by size: 1730 1542 1367 579 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2373 5388 Size: 2373 3015 BclI T'GATC_A Cuts at: 0 2882 3647 5388 Size: 2882 765 1741 Fragments arranged by size: 2882 1741 765 BfrBI ATG'CAT Cuts at: 0 4012 5388 Size: 4012 1376 BglI GCCn_nnn'nGGC Cuts at: 0 1542 1967 3052 4090 5388 Size: 1542 425 1085 1038 1298 Fragments arranged by size: 1542 1298 1085 1038 425 BglII A'GATC_T Cuts at: 0 2918 5388 Size: 2918 2470 BlpI GC'TnA_GC Cuts at: 0 752 1051 1791 1819 5056 5388 Size: 752 299 740 28 3237 332 Fragments arranged by size: 3237 752 740 332 299 28 Bme1580I G_kGCm'C Cuts at: 0 152 836 1202 1527 1533 3040 3057 3345 Size: 152 684 366 325 6 1507 17 288 Cuts at: 3345 3408 3899 4041 4288 4349 4532 4923 5024 Size: 63 491 142 247 61 183 391 101 Cuts at: 5024 5139 5259 5388 Size: 115 120 129 Fragments arranged by size: 1507 684 491 391 366 325 288 247 183 152 142 129 120 115 101 63 61 17 6 BmgBI CAC'GTC Cuts at: 0 597 2848 5388 Size: 597 2251 2540 Fragments arranged by size: 2540 2251 597 BmrI ACTGGGnnnn_n' Cuts at: 0 649 777 1464 1771 4399 5388 Size: 649 128 687 307 2628 989 Fragments arranged by size: 2628 989 687 649 307 128 BmtI G_CTAG'C Cuts at: 0 2305 5388 Size: 2305 3083 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 57 5129 5388 Size: 57 5072 259 Fragments arranged by size: 5072 259 57 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1423 1591 1861 2486 2690 3468 4921 5388 Size: 1423 168 270 625 204 778 1453 467 Fragments arranged by size: 1453 1423 778 625 467 270 204 168 Bpu10I CC'TnA_GC Cuts at: 0 302 731 837 1173 2231 2401 3988 4109 Size: 302 429 106 336 1058 170 1587 121 Cuts at: 4109 5388 Size: 1279 Fragments arranged by size: 1587 1279 1058 429 336 302 170 121 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2305 2551 4731 5305 5388 Size: 2305 246 2180 574 83 Fragments arranged by size: 2305 2180 574 246 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 3023 3378 5364 5388 Size: 3023 355 1986 24 Fragments arranged by size: 3023 1986 355 24 BsaAI yAC'GTr Cuts at: 0 1168 3805 5019 5388 Size: 1168 2637 1214 369 Fragments arranged by size: 2637 1214 1168 369 BsaHI Gr'CG_yC Cuts at: 0 1037 1320 5278 5388 Size: 1037 283 3958 110 Fragments arranged by size: 3958 1037 283 110 BsaWI w'CCGG_w Cuts at: 0 816 5249 5388 Size: 816 4433 139 Fragments arranged by size: 4433 816 139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 2203 2233 4930 4960 5388 Size: 25 2178 30 2697 30 428 Fragments arranged by size: 2697 2178 428 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 2086 2173 2773 3465 3530 4589 4834 Size: 82 2004 87 600 692 65 1059 245 Cuts at: 4834 4837 5388 Size: 3 551 Fragments arranged by size: 2004 1059 692 600 551 245 87 82 65 3 BseYI C'CCAG_C Cuts at: 0 574 1313 1520 2119 2152 2176 3115 3401 Size: 574 739 207 599 33 24 939 286 Cuts at: 3401 3887 4255 4473 4531 4783 4908 4986 5004 Size: 486 368 218 58 252 125 78 18 Cuts at: 5004 5388 Size: 384 Fragments arranged by size: 939 739 599 574 486 384 368 286 252 218 207 125 78 58 33 24 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 573 889 1286 3596 3715 3757 4066 4399 Size: 573 316 397 2310 119 42 309 333 Cuts at: 4399 4843 5179 5388 Size: 444 336 209 Fragments arranged by size: 2310 573 444 397 336 333 316 309 209 119 42 BsiHKAI G_wGCw'C Cuts at: 0 728 816 2165 2450 2706 5024 5388 Size: 728 88 1349 285 256 2318 364 Fragments arranged by size: 2318 1349 728 364 285 256 88 BsiWI C'GTAC_G Cuts at: 0 1164 5388 Size: 1164 4224 BsmI GAATG_Cn' Cuts at: 0 206 2109 3889 4564 4895 5247 5388 Size: 206 1903 1780 675 331 352 141 Fragments arranged by size: 1903 1780 675 352 331 206 141 BsmBI CGTCTCn'nnnn_ Cuts at: 0 763 2278 5083 5388 Size: 763 1515 2805 305 Fragments arranged by size: 2805 1515 763 305 Bsp1286I G_dGCh'C Cuts at: 0 152 654 728 816 836 1202 1527 1533 Size: 152 502 74 88 20 366 325 6 Cuts at: 1533 1792 1930 2165 2450 2706 3040 3057 3345 Size: 259 138 235 285 256 334 17 288 Cuts at: 3345 3408 3475 3522 3899 4041 4288 4349 4532 Size: 63 67 47 377 142 247 61 183 Cuts at: 4532 4578 4923 5024 5062 5139 5259 5388 Size: 46 345 101 38 77 120 129 Fragments arranged by size: 502 377 366 345 334 325 288 285 259 256 247 235 183 152 142 138 129 120 101 88 77 74 67 63 61 47 46 38 20 17 6 BspEI T'CCGG_A Cuts at: 0 816 5249 5388 Size: 816 4433 139 Fragments arranged by size: 4433 816 139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 237 1055 1786 3536 3737 4174 4234 4451 Size: 237 818 731 1750 201 437 60 217 Cuts at: 4451 4874 5138 5388 Size: 423 264 250 Fragments arranged by size: 1750 818 731 437 423 264 250 237 217 201 60 BsrBI CCG'CTC Cuts at: 0 1407 1559 4799 4952 5113 5388 Size: 1407 152 3240 153 161 275 Fragments arranged by size: 3240 1407 275 161 153 152 BsrDI GCAATG_nn' Cuts at: 0 445 1095 2034 5388 Size: 445 650 939 3354 Fragments arranged by size: 3354 939 650 445 BsrFI r'CCGG_y Cuts at: 0 1586 4299 5388 Size: 1586 2713 1089 Fragments arranged by size: 2713 1586 1089 BssSI C'ACGA_G Cuts at: 0 2367 5388 Size: 2367 3021 BstAPI GCAn_nnn'nTGC Cuts at: 0 1240 2059 2782 2785 3088 4866 5388 Size: 1240 819 723 3 303 1778 522 Fragments arranged by size: 1778 1240 819 723 522 303 3 BstEII G'GTnAC_C Cuts at: 0 2860 5388 Size: 2860 2528 BstXI CCAn_nnnn'nTGG Cuts at: 0 1761 2520 4408 4704 4791 5388 Size: 1761 759 1888 296 87 597 Fragments arranged by size: 1888 1761 759 597 296 87 BstYI r'GATC_y Cuts at: 0 737 2295 2573 2918 4028 5388 Size: 737 1558 278 345 1110 1360 Fragments arranged by size: 1558 1360 1110 737 345 278 Bsu36I CC'TnA_GG Cuts at: 0 3072 3699 3911 4271 5388 Size: 3072 627 212 360 1117 Fragments arranged by size: 3072 1117 627 360 212 BtgI C'CryG_G Cuts at: 0 968 3845 4643 5282 5388 Size: 968 2877 798 639 106 Fragments arranged by size: 2877 968 798 639 106 BtsI GCAGTG_nn' Cuts at: 0 192 393 901 1194 1354 1782 2265 3345 Size: 192 201 508 293 160 428 483 1080 Cuts at: 3345 4054 4100 4328 4424 4616 4690 4722 4759 Size: 709 46 228 96 192 74 32 37 Cuts at: 4759 5388 Size: 629 Fragments arranged by size: 1080 709 629 508 483 428 293 228 201 192 192 160 96 74 46 37 32 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 230 265 897 932 5388 Size: 230 35 632 35 4456 Fragments arranged by size: 4456 632 230 35 35 DraIII CAC_nnn'GTG Cuts at: 0 600 1277 1765 2644 4552 5388 Size: 600 677 488 879 1908 836 Fragments arranged by size: 1908 879 836 677 600 488 DrdI GACnn_nn'nnGTC Cuts at: 0 1267 5388 Size: 1267 4121 EaeI y'GGCC_r Cuts at: 0 377 1286 1426 1543 1853 2675 3323 4398 Size: 377 909 140 117 310 822 648 1075 Cuts at: 4398 5372 5388 Size: 974 16 Fragments arranged by size: 1075 974 909 822 648 377 310 140 117 16 EarI CTCTTCn'nnn_ Cuts at: 0 344 424 619 1417 1454 2126 3459 4094 Size: 344 80 195 798 37 672 1333 635 Cuts at: 4094 5388 Size: 1294 Fragments arranged by size: 1333 1294 798 672 635 344 195 80 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 191 1817 5388 Size: 191 1626 3571 Fragments arranged by size: 3571 1626 191 EcoICRI GAG'CTC Cuts at: 0 726 814 5388 Size: 726 88 4574 Fragments arranged by size: 4574 726 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 921 1378 1423 1591 1724 1861 2203 2486 Size: 921 457 45 168 133 137 342 283 Cuts at: 2486 2690 3468 4151 4921 5388 Size: 204 778 683 770 467 Fragments arranged by size: 921 778 770 683 467 457 342 283 204 168 137 133 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 503 2404 5388 Size: 503 1901 2984 Fragments arranged by size: 2984 1901 503 EcoO109I rG'GnC_Cy Cuts at: 0 148 441 509 832 833 1530 1566 1665 Size: 148 293 68 323 1 697 36 99 Cuts at: 1665 1684 2235 3036 3053 3054 3085 3124 3287 Size: 19 551 801 17 1 31 39 163 Cuts at: 3287 3405 3715 4038 4284 5388 Size: 118 310 323 246 1104 Fragments arranged by size: 1104 801 697 551 323 323 310 293 246 163 148 118 99 68 39 36 31 19 17 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2430 2937 5388 Size: 2430 507 2451 Fragments arranged by size: 2451 2430 507 FspI TGC'GCA Cuts at: 0 2246 5388 Size: 2246 3142 FspAI rTGC'GCAy Cuts at: 0 2246 5388 Size: 2246 3142 HaeII r_GCGC'y Cuts at: 0 39 1323 5166 5267 5388 Size: 39 1284 3843 101 121 Fragments arranged by size: 3843 1284 121 101 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 203 235 483 515 641 655 673 687 Size: 203 32 248 32 126 14 18 14 Cuts at: 687 934 966 3904 3922 3936 3954 4818 4850 Size: 247 32 2938 18 14 18 864 32 Cuts at: 4850 4896 4928 5388 Size: 46 32 460 Fragments arranged by size: 2938 864 460 248 247 203 126 46 32 32 32 32 32 18 18 18 14 14 14 HincII GTy'rAC Cuts at: 0 788 2423 5388 Size: 788 1635 2965 Fragments arranged by size: 2965 1635 788 HindIII A'AGCT_T Cuts at: 0 2908 5388 Size: 2908 2480 KasI G'GCGC_C Cuts at: 0 1319 5388 Size: 1319 4069 KpnI G_GTAC'C Cuts at: 0 197 5388 Size: 197 5191 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1085 2046 4055 4866 5388 Size: 1085 961 2009 811 522 Fragments arranged by size: 2009 1085 961 811 522 MscI TGG'CCA Cuts at: 0 379 2677 3325 4400 5374 5388 Size: 379 2298 648 1075 974 14 Fragments arranged by size: 2298 1075 974 648 379 14 MslI CAynn'nnrTG Cuts at: 0 467 1187 1244 2246 3319 4007 4093 4342 Size: 467 720 57 1002 1073 688 86 249 Cuts at: 4342 4357 4621 4702 4868 5132 5287 5388 Size: 15 264 81 166 264 155 101 Fragments arranged by size: 1073 1002 720 688 467 264 264 249 166 155 101 86 81 57 15 MspA1I CmG'CkG Cuts at: 0 145 539 560 693 985 1317 1341 1432 Size: 145 394 21 133 292 332 24 91 Cuts at: 1432 1493 1615 1680 1702 3610 4035 4315 5100 Size: 61 122 65 22 1908 425 280 785 Cuts at: 5100 5355 5388 Size: 255 33 Fragments arranged by size: 1908 785 425 394 332 292 280 255 145 133 122 91 65 61 33 24 22 21 NarI GG'CG_CC Cuts at: 0 1320 5388 Size: 1320 4068 NcoI C'CATG_G Cuts at: 0 3845 4643 5282 5388 Size: 3845 798 639 106 Fragments arranged by size: 3845 798 639 106 NheI G'CTAG_C Cuts at: 0 2301 5388 Size: 2301 3087 NsiI A_TGCA'T Cuts at: 0 4014 5388 Size: 4014 1374 NspI r_CATG'y Cuts at: 0 429 1001 1257 2245 2478 4506 4873 5184 Size: 429 572 256 988 233 2028 367 311 Cuts at: 5184 5388 Size: 204 Fragments arranged by size: 2028 988 572 429 367 311 256 233 204 PasI CC'CwG_GG Cuts at: 0 1887 3049 3468 4288 5388 Size: 1887 1162 419 820 1100 Fragments arranged by size: 1887 1162 1100 820 419 PflMI CCAn_nnn'nTGG Cuts at: 0 600 1477 2457 3416 3846 5154 5388 Size: 600 877 980 959 430 1308 234 Fragments arranged by size: 1308 980 959 877 600 430 234 PfoI T'CCnGG_A Cuts at: 0 761 3826 4383 5388 Size: 761 3065 557 1005 Fragments arranged by size: 3065 1005 761 557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2813 2845 5388 Size: 2813 32 2543 Fragments arranged by size: 2813 2543 32 PpuMI rG'GwC_Cy Cuts at: 0 441 1665 1684 3287 3715 5388 Size: 441 1224 19 1603 428 1673 Fragments arranged by size: 1673 1603 1224 441 428 19 PspOMI G'GGCC_C Cuts at: 0 148 832 1523 1529 3036 3053 3404 4037 Size: 148 684 691 6 1507 17 351 633 Cuts at: 4037 4284 5388 Size: 247 1104 Fragments arranged by size: 1507 1104 691 684 633 351 247 148 17 6 PstI C_TGCA'G Cuts at: 0 559 639 2064 3084 4185 5075 5099 5388 Size: 559 80 1425 1020 1101 890 24 289 Fragments arranged by size: 1425 1101 1020 890 559 289 80 24 PvuII CAG'CTG Cuts at: 0 560 693 1341 1615 1702 3610 5388 Size: 560 133 648 274 87 1908 1778 Fragments arranged by size: 1908 1778 648 560 274 133 87 RsrII CG'GwC_CG Cuts at: 0 57 5388 Size: 57 5331 SacI G_AGCT'C Cuts at: 0 728 816 5388 Size: 728 88 4572 Fragments arranged by size: 4572 728 88 SanDI GG'GwC_CC Cuts at: 0 1665 1684 3287 5388 Size: 1665 19 1603 2101 Fragments arranged by size: 2101 1665 1603 19 SapI GCTCTTCn'nnn_ Cuts at: 0 424 1417 3459 5388 Size: 424 993 2042 1929 Fragments arranged by size: 2042 1929 993 424 ScaI AGT'ACT Cuts at: 0 855 5388 Size: 855 4533 SfcI C'TryA_G Cuts at: 0 310 473 555 635 679 1488 2012 2060 Size: 310 163 82 80 44 809 524 48 Cuts at: 2060 2253 2491 3080 4181 4221 4432 4449 5071 Size: 193 238 589 1101 40 211 17 622 Cuts at: 5071 5095 5311 5388 Size: 24 216 77 Fragments arranged by size: 1101 809 622 589 524 310 238 216 211 193 163 82 80 77 48 44 40 24 17 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1967 5388 Size: 1967 3421 SfoI GGC'GCC Cuts at: 0 1321 5388 Size: 1321 4067 SmaI CCC'GGG Cuts at: 0 1528 1582 3523 4379 5388 Size: 1528 54 1941 856 1009 Fragments arranged by size: 1941 1528 1009 856 54 SmlI C'TyrA_G Cuts at: 0 2320 2566 2911 3375 4710 5320 5365 5388 Size: 2320 246 345 464 1335 610 45 23 Fragments arranged by size: 2320 1335 610 464 345 246 45 23 SnaBI TAC'GTA Cuts at: 0 1168 5388 Size: 1168 4220 SphI G_CATG'C Cuts at: 0 429 2245 4873 5388 Size: 429 1816 2628 515 Fragments arranged by size: 2628 1816 515 429 SrfI GCCC'GGGC Cuts at: 0 1528 3523 5388 Size: 1528 1995 1865 Fragments arranged by size: 1995 1865 1528 StuI AGG'CCT Cuts at: 0 1441 1465 2266 2499 2518 4084 5388 Size: 1441 24 801 233 19 1566 1304 Fragments arranged by size: 1566 1441 1304 801 233 24 19 StyI C'CwwG_G Cuts at: 0 1625 1754 2342 2483 2513 2519 2789 3214 Size: 1625 129 588 141 30 6 270 425 Cuts at: 3214 3765 3845 4569 4643 4749 5045 5282 5339 Size: 551 80 724 74 106 296 237 57 Cuts at: 5339 5388 Size: 49 Fragments arranged by size: 1625 724 588 551 425 296 270 237 141 129 106 80 74 57 49 30 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 607 1645 1753 2373 2954 4487 4665 5388 Size: 607 1038 108 620 581 1533 178 723 Fragments arranged by size: 1533 1038 723 620 607 581 178 108 TatI w'GTAC_w Cuts at: 0 853 4659 5186 5388 Size: 853 3806 527 202 Fragments arranged by size: 3806 853 527 202 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2628 2660 4647 4679 5388 Size: 2628 32 1987 32 709 Fragments arranged by size: 2628 1987 709 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1037 2116 2729 5388 Size: 1037 1079 613 2659 Fragments arranged by size: 2659 1079 1037 613 XhoI C'TCGA_G Cuts at: 0 5365 5388 Size: 5365 23 XmaI C'CCGG_G Cuts at: 0 1526 1580 3521 4377 5388 Size: 1526 54 1941 856 1011 Fragments arranged by size: 1941 1526 1011 856 54 Enzymes that do cut: AarI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NheI NsiI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SanDI SapI ScaI SfcI SfiI SfoI SmaI SmlI SnaBI SphI SrfI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AgeI ApoI AscI AseI AsiSI BaeI BamHI BsaBI BsiEI BspHI BsrGI BssHII BstBI BstZ17I BtgZI ClaI DraI EagI EcoRI EcoRV FseI HpaI MfeI MluI NaeI NdeI NgoMIV NotI NruI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI SacII SalI SbfI SexAI SgfI SgrAI SpeI SspI SwaI Tth111I XbaI XmnI ZraI