Restriction map for
pF1KA0914
(Linear) (Six-base) MAPSORT of: KIAA0914.seq Check: 5103 from: 1 to: 2007
KIAA0914.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1023 1497 2007
Size: 1023 474 510
Fragments arranged by size:
1023 510 474
Acc65I G'GTAC_C
Cuts at: 0 1532 2007
Size: 1532 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1937 2007
Size: 1223 714 70
Fragments arranged by size:
1223 714 70
AleI CACnn'nnGTG
Cuts at: 0 922 2007
Size: 922 1085
AlwNI CAG_nnn'CTG
Cuts at: 0 202 843 2007
Size: 202 641 1164
Fragments arranged by size:
1164 641 202
ApaLI G'TGCA_C
Cuts at: 0 1658 2007
Size: 1658 349
ApoI r'AATT_y
Cuts at: 0 1162 2007
Size: 1162 845
AvaI C'yCGr_G
Cuts at: 0 1559 2007
Size: 1559 448
BanI G'GyrC_C
Cuts at: 0 824 1532 2007
Size: 824 708 475
Fragments arranged by size:
824 708 475
BanII G_rGCy'C
Cuts at: 0 604 2007
Size: 604 1403
BbeI G_GCGC'C
Cuts at: 0 828 2007
Size: 828 1179
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1610 1862 1904 2007
Size: 857 753 252 42 103
Fragments arranged by size:
857 753 252 103 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1639 1673 2007
Size: 1639 34 334
Fragments arranged by size:
1639 334 34
Bme1580I G_kGCm'C
Cuts at: 0 704 1662 2007
Size: 704 958 345
Fragments arranged by size:
958 704 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 699 750 1316 2007
Size: 699 51 566 691
Fragments arranged by size:
699 691 566 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 422 1345 1786 1984 2007
Size: 194 228 923 441 198 23
Fragments arranged by size:
923 441 228 198 194 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 2007
Size: 1291 716
BsaHI Gr'CG_yC
Cuts at: 0 825 2007
Size: 825 1182
BsaWI w'CCGG_w
Cuts at: 0 106 1058 1128 1166 2007
Size: 106 952 70 38 841
Fragments arranged by size:
952 841 106 70 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1772 1802 1943 1973 2007
Size: 1772 30 141 30 34
Fragments arranged by size:
1772 141 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 756 863 963 1606 2007
Size: 694 62 107 100 643 401
Fragments arranged by size:
694 643 401 107 100 62
BseYI C'CCAG_C
Cuts at: 0 628 820 1019 2007
Size: 628 192 199 988
Fragments arranged by size:
988 628 199 192
BsiEI CG_ry'CG
Cuts at: 0 1486 2007
Size: 1486 521
BsiHKAI G_wGCw'C
Cuts at: 0 75 1662 1973 2007
Size: 75 1587 311 34
Fragments arranged by size:
1587 311 75 34
BsmI GAATG_Cn'
Cuts at: 0 1472 2007
Size: 1472 535
Bsp1286I G_dGCh'C
Cuts at: 0 75 604 704 1662 1973 2007
Size: 75 529 100 958 311 34
Fragments arranged by size:
958 529 311 100 75 34
BspEI T'CCGG_A
Cuts at: 0 1058 1128 1166 2007
Size: 1058 70 38 841
Fragments arranged by size:
1058 841 70 38
BspHI T'CATG_A
Cuts at: 0 456 510 956 2007
Size: 456 54 446 1051
Fragments arranged by size:
1051 456 446 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1023 1497 2007
Size: 1023 474 510
Fragments arranged by size:
1023 510 474
BsrBI CCG'CTC
Cuts at: 0 1375 2007
Size: 1375 632
BsrDI GCAATG_nn'
Cuts at: 0 99 1620 2007
Size: 99 1521 387
Fragments arranged by size:
1521 387 99
BsrFI r'CCGG_y
Cuts at: 0 667 1485 1535 2007
Size: 667 818 50 472
Fragments arranged by size:
818 667 472 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 223 2007
Size: 223 1784
BstBI TT'CG_AA
Cuts at: 0 1079 1682 1833 2007
Size: 1079 603 151 174
Fragments arranged by size:
1079 603 174 151
BstEII G'GTnAC_C
Cuts at: 0 670 2007
Size: 670 1337
BstYI r'GATC_y
Cuts at: 0 614 1055 1550 2007
Size: 614 441 495 457
Fragments arranged by size:
614 495 457 441
Bsu36I CC'TnA_GG
Cuts at: 0 1391 2007
Size: 1391 616
DrdI GACnn_nn'nnGTC
Cuts at: 0 1633 2007
Size: 1633 374
EaeI y'GGCC_r
Cuts at: 0 1483 2007
Size: 1483 524
EagI C'GGCC_G
Cuts at: 0 1483 2007
Size: 1483 524
EarI CTCTTCn'nnn_
Cuts at: 0 740 1079 1199 1583 2007
Size: 740 339 120 384 424
Fragments arranged by size:
740 424 384 339 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 422 1223 1345 1786 1937 1984 2007
Size: 194 228 801 122 441 151 47 23
Fragments arranged by size:
801 441 228 194 151 122 47 23
EcoO109I rG'GnC_Cy
Cuts at: 0 983 1005 1209 2007
Size: 983 22 204 798
Fragments arranged by size:
983 798 204 22
EcoRV GAT'ATC
Cuts at: 0 1199 2007
Size: 1199 808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1644 2007
Size: 1644 363
HaeII r_GCGC'y
Cuts at: 0 246 828 2007
Size: 246 582 1179
Fragments arranged by size:
1179 582 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 1582 1614 1984 2007
Size: 184 32 1366 32 370 23
Fragments arranged by size:
1366 370 184 32 32 23
KasI G'GCGC_C
Cuts at: 0 824 2007
Size: 824 1183
KpnI G_GTAC'C
Cuts at: 0 1536 2007
Size: 1536 471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 2007
Size: 689 1318
MslI CAynn'nnrTG
Cuts at: 0 338 922 1734 1909 2007
Size: 338 584 812 175 98
Fragments arranged by size:
812 584 338 175 98
MspA1I CmG'CkG
Cuts at: 0 139 777 840 2007
Size: 139 638 63 1167
Fragments arranged by size:
1167 638 139 63
NarI GG'CG_CC
Cuts at: 0 825 2007
Size: 825 1182
PfoI T'CCnGG_A
Cuts at: 0 617 1361 1790 1802 1961 2007
Size: 617 744 429 12 159 46
Fragments arranged by size:
744 617 429 159 46 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 775 807 1245 1277 2007
Size: 775 32 438 32 730
Fragments arranged by size:
775 730 438 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 983 1005 1209 2007
Size: 983 22 204 798
Fragments arranged by size:
983 798 204 22
PshAI GACnn'nnGTC
Cuts at: 0 51 2007
Size: 51 1956
PstI C_TGCA'G
Cuts at: 0 1448 2007
Size: 1448 559
PvuII CAG'CTG
Cuts at: 0 139 840 2007
Size: 139 701 1167
Fragments arranged by size:
1167 701 139
SapI GCTCTTCn'nnn_
Cuts at: 0 740 2007
Size: 740 1267
ScaI AGT'ACT
Cuts at: 0 438 2007
Size: 438 1569
SfcI C'TryA_G
Cuts at: 0 140 1444 2007
Size: 140 1304 563
Fragments arranged by size:
1304 563 140
SfoI GGC'GCC
Cuts at: 0 826 2007
Size: 826 1181
SmlI C'TyrA_G
Cuts at: 0 1270 2007
Size: 1270 737
StyI C'CwwG_G
Cuts at: 0 257 2007
Size: 257 1750
TatI w'GTAC_w
Cuts at: 0 436 1094 2007
Size: 436 658 913
Fragments arranged by size:
913 658 436
XmnI GAAnn'nnTTC
Cuts at: 0 1346 1472 2007
Size: 1346 126 535
Fragments arranged by size:
1346 535 126
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BcgI Bme1580I BmrI BpmI
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstBI BstEII BstYI Bsu36I DrdI EaeI EagI
EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I KasI
KpnI MmeI MslI MspA1I NarI PfoI PpiI PpuMI
PshAI PstI PvuII SapI ScaI SfcI SfoI SmlI
StyI TatI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsgI
BsiWI BsmBI BsrGI BssHII BssSI BstXI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI