Restriction map for
pF1KSDA0920
(Linear) (Six-base) MAPSORT of: KIAA0920.seq Check: 6167 from: 1 to: 3309
KIAA0920.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1125 2436 3309
Size: 1125 1311 873
Fragments arranged by size:
1311 1125 873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 355 1310 2522 2543 2651 2920 3056
Size: 230 125 955 1212 21 108 269 136
Cuts at: 3056 3089 3309
Size: 33 220
Fragments arranged by size:
1212 955 269 230 220 136 125 108
33 21
AhdI GACnn_n'nnGTC
Cuts at: 0 2124 3309
Size: 2124 1185
AlwNI CAG_nnn'CTG
Cuts at: 0 258 934 1527 1588 2670 3309
Size: 258 676 593 61 1082 639
Fragments arranged by size:
1082 676 639 593 258 61
ApaI G_GGCC'C
Cuts at: 0 1943 1970 2660 3309
Size: 1943 27 690 649
Fragments arranged by size:
1943 690 649 27
ApoI r'AATT_y
Cuts at: 0 1695 1999 2238 3309
Size: 1695 304 239 1071
Fragments arranged by size:
1695 1071 304 239
AvaI C'yCGr_G
Cuts at: 0 1181 2012 3217 3309
Size: 1181 831 1205 92
Fragments arranged by size:
1205 1181 831 92
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2135 2168 3309
Size: 2135 33 1141
Fragments arranged by size:
2135 1141 33
BanI G'GyrC_C
Cuts at: 0 1023 1137 1843 1876 3309
Size: 1023 114 706 33 1433
Fragments arranged by size:
1433 1023 706 114 33
BanII G_rGCy'C
Cuts at: 0 1043 1367 1943 1970 2660 2938 3309
Size: 1043 324 576 27 690 278 371
Fragments arranged by size:
1043 690 576 371 324 278 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1841 2573 2639 3128 3305 3309
Size: 1841 732 66 489 177 4
Fragments arranged by size:
1841 732 489 177 66 4
BbvCI CC'TCA_GC
Cuts at: 0 1848 1988 2824 3309
Size: 1848 140 836 485
Fragments arranged by size:
1848 836 485 140
BciVI GTATCCnnnnn_n'
Cuts at: 0 2136 3309
Size: 2136 1173
BclI T'GATC_A
Cuts at: 0 810 2328 3309
Size: 810 1518 981
Fragments arranged by size:
1518 981 810
BglII A'GATC_T
Cuts at: 0 2071 3309
Size: 2071 1238
BlpI GC'TnA_GC
Cuts at: 0 2756 3309
Size: 2756 553
Bme1580I G_kGCm'C
Cuts at: 0 1512 1881 1943 1970 2660 3309
Size: 1512 369 62 27 690 649
Fragments arranged by size:
1512 690 649 369 62 27
BmgBI CAC'GTC
Cuts at: 0 2802 3309
Size: 2802 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 2681 3309
Size: 2681 628
BmtI G_CTAG'C
Cuts at: 0 1016 3309
Size: 1016 2293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 555 2124 3309
Size: 555 1569 1185
Fragments arranged by size:
1569 1185 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 363 515 673 1360 1489 1814 1903
Size: 280 83 152 158 687 129 325 89
Cuts at: 1903 2176 2215 2952 2975 3309
Size: 273 39 737 23 334
Fragments arranged by size:
737 687 334 325 280 273 158 152
129 89 83 39 23
Bpu10I CC'TnA_GC
Cuts at: 0 961 1848 1988 2644 2824 2879 3309
Size: 961 887 140 656 180 55 430
Fragments arranged by size:
961 887 656 430 180 140 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2052 2774 3309
Size: 2052 722 535
Fragments arranged by size:
2052 722 535
BsaI GGTCTCn'nnnn_
Cuts at: 0 1800 1879 2141 2152 2704 3309
Size: 1800 79 262 11 552 605
Fragments arranged by size:
1800 605 552 262 79 11
BsaHI Gr'CG_yC
Cuts at: 0 2309 3309
Size: 2309 1000
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 397 413 427 754 784 1210 1240
Size: 383 14 16 14 327 30 426 30
Cuts at: 1240 2294 2324 3309
Size: 1054 30 985
Fragments arranged by size:
1054 985 426 383 327 30 30 30
16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1019 1345 1371 1459 2113 2315 2722
Size: 199 820 326 26 88 654 202 407
Cuts at: 2722 2908 3008 3309
Size: 186 100 301
Fragments arranged by size:
820 654 407 326 301 202 199 186
100 88 26
BseYI C'CCAG_C
Cuts at: 0 416 590 986 1099 1119 1150 2358 2663
Size: 416 174 396 113 20 31 1208 305
Cuts at: 2663 3043 3086 3263 3309
Size: 380 43 177 46
Fragments arranged by size:
1208 416 396 380 305 177 174 113
46 43 31 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1563 2434 2701 3309
Size: 959 604 871 267 608
Fragments arranged by size:
959 871 608 604 267
BsiHKAI G_wGCw'C
Cuts at: 0 1043 1367 3309
Size: 1043 324 1942
Fragments arranged by size:
1942 1043 324
BsmI GAATG_Cn'
Cuts at: 0 117 2424 2955 3309
Size: 117 2307 531 354
Fragments arranged by size:
2307 531 354 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2316 3309
Size: 2316 993
Bsp1286I G_dGCh'C
Cuts at: 0 1043 1367 1512 1881 1943 1970 2660 2938
Size: 1043 324 145 369 62 27 690 278
Cuts at: 2938 3309
Size: 371
Fragments arranged by size:
1043 690 371 369 324 278 145 62
27
BspHI T'CATG_A
Cuts at: 0 1599 3309
Size: 1599 1710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1125 2436 3309
Size: 437 688 1311 873
Fragments arranged by size:
1311 873 688 437
BsrBI CCG'CTC
Cuts at: 0 490 3309
Size: 490 2819
BsrDI GCAATG_nn'
Cuts at: 0 1400 3309
Size: 1400 1909
BsrFI r'CCGG_y
Cuts at: 0 1016 3309
Size: 1016 2293
BssSI C'ACGA_G
Cuts at: 0 976 3309
Size: 976 2333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 2154 3309
Size: 651 1503 1155
Fragments arranged by size:
1503 1155 651
BstYI r'GATC_y
Cuts at: 0 848 2071 2867 3275 3309
Size: 848 1223 796 408 34
Fragments arranged by size:
1223 848 796 408 34
Bsu36I CC'TnA_GG
Cuts at: 0 2573 3309
Size: 2573 736
BtgI C'CryG_G
Cuts at: 0 758 1064 2046 3309
Size: 758 306 982 1263
Fragments arranged by size:
1263 982 758 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1553 3309
Size: 1553 1756
BtsI GCAGTG_nn'
Cuts at: 0 947 2668 2959 3309
Size: 947 1721 291 350
Fragments arranged by size:
1721 947 350 291
EaeI y'GGCC_r
Cuts at: 0 1089 1712 2783 3309
Size: 1089 623 1071 526
Fragments arranged by size:
1089 1071 623 526
EarI CTCTTCn'nnn_
Cuts at: 0 176 206 284 1599 2858 2885 2896 3065
Size: 176 30 78 1315 1259 27 11 169
Cuts at: 3065 3309
Size: 244
Fragments arranged by size:
1315 1259 244 176 169 78 30 27
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 3309
Size: 25 3284
EcoICRI GAG'CTC
Cuts at: 0 1041 1365 3309
Size: 1041 324 1944
Fragments arranged by size:
1944 1041 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 280 355 363 515 673 1310 1360
Size: 230 50 75 8 152 158 637 50
Cuts at: 1360 1489 1814 1903 2176 2215 2522 2543 2651
Size: 129 325 89 273 39 307 21 108
Cuts at: 2651 2920 2952 2975 3056 3089 3309
Size: 269 32 23 81 33 220
Fragments arranged by size:
637 325 307 273 269 230 220 158
152 129 108 89 81 75 50 50
39 33 32 23 21 8
EcoO109I rG'GnC_Cy
Cuts at: 0 1825 1939 1940 1967 2497 2656 2657 3040
Size: 1825 114 1 27 530 159 1 383
Cuts at: 3040 3213 3309
Size: 173 96
Fragments arranged by size:
1825 530 383 173 159 114 96 27
1 1
EcoRI G'AATT_C
Cuts at: 0 1695 3309
Size: 1695 1614
EcoRV GAT'ATC
Cuts at: 0 302 609 3309
Size: 302 307 2700
Fragments arranged by size:
2700 307 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2119 3309
Size: 2119 1190
HaeII r_GCGC'y
Cuts at: 0 2012 2644 3309
Size: 2012 632 665
Fragments arranged by size:
2012 665 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 840 872 1352 1384 1876 1908
Size: 754 32 54 32 480 32 492 32
Cuts at: 1908 2043 2050 2075 2082 2110 2142 2149 2181
Size: 135 7 25 7 28 32 7 32
Cuts at: 2181 2272 2294 2304 2326 3309
Size: 91 22 10 22 983
Fragments arranged by size:
983 754 492 480 135 91 54 32
32 32 32 32 32 28 25 22
22 10 7 7 7
HincII GTy'rAC
Cuts at: 0 1779 3309
Size: 1779 1530
MscI TGG'CCA
Cuts at: 0 1091 1714 2785 3309
Size: 1091 623 1071 524
Fragments arranged by size:
1091 1071 623 524
MspA1I CmG'CkG
Cuts at: 0 169 1452 1686 2201 2677 2755 2841 3090
Size: 169 1283 234 515 476 78 86 249
Cuts at: 3090 3309
Size: 219
Fragments arranged by size:
1283 515 476 249 234 219 169 86
78
NaeI GCC'GGC
Cuts at: 0 1018 3309
Size: 1018 2291
NcoI C'CATG_G
Cuts at: 0 758 1064 2046 3309
Size: 758 306 982 1263
Fragments arranged by size:
1263 982 758 306
NgoMIV G'CCGG_C
Cuts at: 0 1016 3309
Size: 1016 2293
NheI G'CTAG_C
Cuts at: 0 1012 3309
Size: 1012 2297
NspI r_CATG'y
Cuts at: 0 2562 2978 3309
Size: 2562 416 331
Fragments arranged by size:
2562 416 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 934 3309
Size: 466 468 2375
Fragments arranged by size:
2375 468 466
PfoI T'CCnGG_A
Cuts at: 0 650 732 2153 2231 2830 3309
Size: 650 82 1421 78 599 479
Fragments arranged by size:
1421 650 599 479 82 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 3309
Size: 205 32 3072
Fragments arranged by size:
3072 205 32
PpuMI rG'GwC_Cy
Cuts at: 0 1825 2497 3213 3309
Size: 1825 672 716 96
Fragments arranged by size:
1825 716 672 96
PshAI GACnn'nnGTC
Cuts at: 0 2111 3309
Size: 2111 1198
PspOMI G'GGCC_C
Cuts at: 0 1939 1966 2656 3309
Size: 1939 27 690 653
Fragments arranged by size:
1939 690 653 27
PspXI vC'TCGA_Gb
Cuts at: 0 3217 3309
Size: 3217 92
PstI C_TGCA'G
Cuts at: 0 116 158 1136 1535 3053 3309
Size: 116 42 978 399 1518 256
Fragments arranged by size:
1518 978 399 256 116 42
PvuII CAG'CTG
Cuts at: 0 1452 1686 2677 2755 3309
Size: 1452 234 991 78 554
Fragments arranged by size:
1452 991 554 234 78
SacI G_AGCT'C
Cuts at: 0 1043 1367 3309
Size: 1043 324 1942
Fragments arranged by size:
1942 1043 324
SbfI CC_TGCA'GG
Cuts at: 0 1136 3309
Size: 1136 2173
ScaI AGT'ACT
Cuts at: 0 2743 3309
Size: 2743 566
SfcI C'TryA_G
Cuts at: 0 112 154 636 1132 1531 1758 1944 2510
Size: 112 42 482 496 399 227 186 566
Cuts at: 2510 3049 3309
Size: 539 260
Fragments arranged by size:
566 539 496 482 399 260 227 186
112 42
SmaI CCC'GGG
Cuts at: 0 2014 3309
Size: 2014 1295
SmlI C'TyrA_G
Cuts at: 0 2067 2789 3217 3309
Size: 2067 722 428 92
Fragments arranged by size:
2067 722 428 92
SrfI GCCC'GGGC
Cuts at: 0 2014 3309
Size: 2014 1295
StuI AGG'CCT
Cuts at: 0 1323 1764 3309
Size: 1323 441 1545
Fragments arranged by size:
1545 1323 441
StyI C'CwwG_G
Cuts at: 0 758 1064 1715 1856 2046 2387 3309
Size: 758 306 651 141 190 341 922
Fragments arranged by size:
922 758 651 341 306 190 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1746 3309
Size: 1746 1563
TatI w'GTAC_w
Cuts at: 0 1484 1726 2741 3309
Size: 1484 242 1015 568
Fragments arranged by size:
1484 1015 568 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 805 837 3309
Size: 805 32 2472
Fragments arranged by size:
2472 805 32
Tth111I GACn'n_nGTC
Cuts at: 0 1639 2847 3309
Size: 1639 1208 462
Fragments arranged by size:
1639 1208 462
XbaI T'CTAG_A
Cuts at: 0 858 3309
Size: 858 2451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1707 3309
Size: 1707 1602
XhoI C'TCGA_G
Cuts at: 0 3217 3309
Size: 3217 92
XmaI C'CCGG_G
Cuts at: 0 2012 3309
Size: 2012 1297
XmnI GAAnn'nnTTC
Cuts at: 0 373 3309
Size: 373 2936
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII MscI MspA1I NaeI NcoI NgoMIV NheI
NspI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SbfI ScaI SfcI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BfrBI BglI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoNI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NdeI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PsiI PsrI PvuI
RsrII SacII SalI SanDI SapI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI ZraI