Restriction map for
pF1KSDA0923
(Linear) (Six-base) MAPSORT of: KIAA0923.seq Check: 5504 from: 1 to: 1842
KIAA0923.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 633 1842
Size: 633 1209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 310 448 643 761 1613 1718 1842
Size: 23 287 138 195 118 852 105 124
Fragments arranged by size:
852 287 195 138 124 118 105 23
AflII C'TTAA_G
Cuts at: 0 421 829 1842
Size: 421 408 1013
Fragments arranged by size:
1013 421 408
AflIII A'CryG_T
Cuts at: 0 1645 1842
Size: 1645 197
AlwNI CAG_nnn'CTG
Cuts at: 0 925 1842
Size: 925 917
ApaLI G'TGCA_C
Cuts at: 0 145 1528 1842
Size: 145 1383 314
Fragments arranged by size:
1383 314 145
ApoI r'AATT_y
Cuts at: 0 369 501 770 1040 1166 1332 1812 1842
Size: 369 132 269 270 126 166 480 30
Fragments arranged by size:
480 369 270 269 166 132 126 30
AvaI C'yCGr_G
Cuts at: 0 1156 1533 1842
Size: 1156 377 309
Fragments arranged by size:
1156 377 309
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 1842
Size: 29 1813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 126 160 1842
Size: 126 34 1682
Fragments arranged by size:
1682 126 34
BclI T'GATC_A
Cuts at: 0 207 1842
Size: 207 1635
Bme1580I G_kGCm'C
Cuts at: 0 149 1532 1842
Size: 149 1383 310
Fragments arranged by size:
1383 310 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 744 1562 1842
Size: 426 318 818 280
Fragments arranged by size:
818 426 318 280
Bpu10I CC'TnA_GC
Cuts at: 0 347 1842
Size: 347 1495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1821 1842
Size: 1821 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1580 1842
Size: 1580 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 717 747 1842
Size: 717 30 1095
Fragments arranged by size:
1095 717 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1739 1842
Size: 1739 103
BseYI C'CCAG_C
Cuts at: 0 378 1842
Size: 378 1464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1842
Size: 153 1689
BsiHKAI G_wGCw'C
Cuts at: 0 149 1532 1842
Size: 149 1383 310
Fragments arranged by size:
1383 310 149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1297 1341 1842
Size: 1297 44 501
Fragments arranged by size:
1297 501 44
Bsp1286I G_dGCh'C
Cuts at: 0 149 1532 1842
Size: 149 1383 310
Fragments arranged by size:
1383 310 149
BspHI T'CATG_A
Cuts at: 0 585 1842
Size: 585 1257
BsrDI GCAATG_nn'
Cuts at: 0 557 1842
Size: 557 1285
BsrGI T'GTAC_A
Cuts at: 0 1732 1842
Size: 1732 110
BstYI r'GATC_y
Cuts at: 0 24 1842
Size: 24 1818
BtsI GCAGTG_nn'
Cuts at: 0 1180 1842
Size: 1180 662
DraI TTT'AAA
Cuts at: 0 1317 1842
Size: 1317 525
DraIII CAC_nnn'GTG
Cuts at: 0 1563 1842
Size: 1563 279
EarI CTCTTCn'nnn_
Cuts at: 0 589 875 1613 1842
Size: 589 286 738 229
Fragments arranged by size:
738 589 286 229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 310 426 448 643 744 761 1562
Size: 23 287 116 22 195 101 17 801
Cuts at: 1562 1613 1718 1842
Size: 51 105 124
Fragments arranged by size:
801 287 195 124 116 105 101 51
23 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 444 1842
Size: 444 1398
EcoO109I rG'GnC_Cy
Cuts at: 0 981 1842
Size: 981 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1179 1211 1842
Size: 1105 32 42 32 631
Fragments arranged by size:
1105 631 42 32 32
HindIII A'AGCT_T
Cuts at: 0 1063 1353 1842
Size: 1063 290 489
Fragments arranged by size:
1063 489 290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 1701 1842
Size: 1256 445 141
Fragments arranged by size:
1256 445 141
MspA1I CmG'CkG
Cuts at: 0 330 1842
Size: 330 1512
NdeI CA'TA_TG
Cuts at: 0 1233 1842
Size: 1233 609
NspI r_CATG'y
Cuts at: 0 1590 1842
Size: 1590 252
PfoI T'CCnGG_A
Cuts at: 0 442 721 1842
Size: 442 279 1121
Fragments arranged by size:
1121 442 279
PpuMI rG'GwC_Cy
Cuts at: 0 981 1842
Size: 981 861
PspXI vC'TCGA_Gb
Cuts at: 0 1156 1533 1842
Size: 1156 377 309
Fragments arranged by size:
1156 377 309
SfcI C'TryA_G
Cuts at: 0 400 1785 1842
Size: 400 1385 57
Fragments arranged by size:
1385 400 57
SmlI C'TyrA_G
Cuts at: 0 421 829 1156 1533 1800 1842
Size: 421 408 327 377 267 42
Fragments arranged by size:
421 408 377 327 267 42
SspI AAT'ATT
Cuts at: 0 183 1842
Size: 183 1659
TatI w'GTAC_w
Cuts at: 0 294 916 1680 1732 1842
Size: 294 622 764 52 110
Fragments arranged by size:
764 622 294 110 52
XhoI C'TCGA_G
Cuts at: 0 1156 1533 1842
Size: 1156 377 309
Fragments arranged by size:
1156 377 309
XmnI GAAnn'nnTTC
Cuts at: 0 1483 1842
Size: 1483 359
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaLI ApoI AvaI
BbsI BcgI BclI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BsrDI BsrGI BstYI BtsI DraI DraIII EarI Eco57MI
EcoNI EcoO109I Hin4I HindIII MmeI MspA1I NdeI NspI
PfoI PpuMI PspXI SfcI SmlI SspI TatI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DrdI EaeI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI ZraI