Restriction map for
pF1KA0926
(Linear) (Six-base) MAPSORT of: KIAA0926.seq Check: 4967 from: 1 to: 4287
KIAA0926.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1026 4287
Size: 1026 3261
AccI GT'mk_AC
Cuts at: 0 1653 4287
Size: 1653 2634
AclI AA'CG_TT
Cuts at: 0 2185 4287
Size: 2185 2102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 82 1083 2284 2449 2791 2934 4231
Size: 67 15 1001 1201 165 342 143 1297
Cuts at: 4231 4287
Size: 56
Fragments arranged by size:
1297 1201 1001 342 165 143 67 56
15
AflIII A'CryG_T
Cuts at: 0 2011 4287
Size: 2011 2276
AgeI A'CCGG_T
Cuts at: 0 3156 4287
Size: 3156 1131
AhdI GACnn_n'nnGTC
Cuts at: 0 498 4287
Size: 498 3789
AleI CACnn'nnGTG
Cuts at: 0 343 541 3697 4287
Size: 343 198 3156 590
Fragments arranged by size:
3156 590 343 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1019 4287
Size: 1019 3268
AlwNI CAG_nnn'CTG
Cuts at: 0 78 1194 2127 2598 2664 2694 2739 2910
Size: 78 1116 933 471 66 30 45 171
Cuts at: 2910 3405 4002 4089 4287
Size: 495 597 87 198
Fragments arranged by size:
1116 933 597 495 471 198 171 87
78 66 45 30
ApaI G_GGCC'C
Cuts at: 0 1545 2919 3244 4287
Size: 1545 1374 325 1043
Fragments arranged by size:
1545 1374 1043 325
ApaLI G'TGCA_C
Cuts at: 0 381 2259 3490 4287
Size: 381 1878 1231 797
Fragments arranged by size:
1878 1231 797 381
ApoI r'AATT_y
Cuts at: 0 638 829 2275 3382 4287
Size: 638 191 1446 1107 905
Fragments arranged by size:
1446 1107 905 638 191
AvaI C'yCGr_G
Cuts at: 0 1348 4040 4287
Size: 1348 2692 247
Fragments arranged by size:
2692 1348 247
BamHI G'GATC_C
Cuts at: 0 359 559 4287
Size: 359 200 3728
Fragments arranged by size:
3728 359 200
BanII G_rGCy'C
Cuts at: 0 109 394 1148 1277 1538 1545 2252 2341
Size: 109 285 754 129 261 7 707 89
Cuts at: 2341 2837 2919 3244 3794 4287
Size: 496 82 325 550 493
Fragments arranged by size:
754 707 550 496 493 325 285 261
129 109 89 82 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 1083 3821 4287
Size: 145 938 2738 466
Fragments arranged by size:
2738 938 466 145
BbvCI CC'TCA_GC
Cuts at: 0 235 569 1727 2552 2936 4287
Size: 235 334 1158 825 384 1351
Fragments arranged by size:
1351 1158 825 384 334 235
BciVI GTATCCnnnnn_n'
Cuts at: 0 960 2988 4287
Size: 960 2028 1299
Fragments arranged by size:
2028 1299 960
BclI T'GATC_A
Cuts at: 0 1364 4287
Size: 1364 2923
BglI GCCn_nnn'nGGC
Cuts at: 0 1174 1209 4287
Size: 1174 35 3078
Fragments arranged by size:
3078 1174 35
BglII A'GATC_T
Cuts at: 0 3107 3927 4287
Size: 3107 820 360
Fragments arranged by size:
3107 820 360
BlpI GC'TnA_GC
Cuts at: 0 128 1142 1682 2458 3467 4090 4287
Size: 128 1014 540 776 1009 623 197
Fragments arranged by size:
1014 1009 776 623 540 197 128
Bme1580I G_kGCm'C
Cuts at: 0 385 1545 1559 2263 2919 3244 3494 4287
Size: 385 1160 14 704 656 325 250 793
Fragments arranged by size:
1160 793 704 656 385 325 250 14
BmgBI CAC'GTC
Cuts at: 0 2720 3099 4287
Size: 2720 379 1188
Fragments arranged by size:
2720 1188 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 1113 2365 4287
Size: 1113 1252 1922
Fragments arranged by size:
1922 1252 1113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 802 2519 3156 4287
Size: 177 625 1717 637 1131
Fragments arranged by size:
1717 1131 637 625 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 462 1308 2167 2536 2660 2941 3494
Size: 84 378 846 859 369 124 281 553
Cuts at: 3494 3601 3808 3830 3941 4287
Size: 107 207 22 111 346
Fragments arranged by size:
859 846 553 378 369 346 281 207
124 111 107 84 22
Bpu10I CC'TnA_GC
Cuts at: 0 235 569 1727 2552 2614 2836 2936 3074
Size: 235 334 1158 825 62 222 100 138
Cuts at: 3074 4287
Size: 1213
Fragments arranged by size:
1213 1158 825 334 235 222 138 100
62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2395 3013 3176 4182 4287
Size: 2395 618 163 1006 105
Fragments arranged by size:
2395 1006 618 163 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 323 1700 2512 3346 4210 4287
Size: 323 1377 812 834 864 77
Fragments arranged by size:
1377 864 834 812 323 77
BsaAI yAC'GTr
Cuts at: 0 2289 4287
Size: 2289 1998
BsaHI Gr'CG_yC
Cuts at: 0 435 2499 4287
Size: 435 2064 1788
Fragments arranged by size:
2064 1788 435
BsaWI w'CCGG_w
Cuts at: 0 3156 4178 4287
Size: 3156 1022 109
Fragments arranged by size:
3156 1022 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1155 1161 1185 1191 4287
Size: 1155 6 24 6 3096
Fragments arranged by size:
3096 1155 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 454 928 3580 4287
Size: 70 384 474 2652 707
Fragments arranged by size:
2652 707 474 384 70
BseYI C'CCAG_C
Cuts at: 0 322 546 554 574 1317 1329 1697 2764
Size: 322 224 8 20 743 12 368 1067
Cuts at: 2764 3628 4135 4287
Size: 864 507 152
Fragments arranged by size:
1067 864 743 507 368 322 224 152
20 12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1563 3988 4287
Size: 1267 296 2425 299
Fragments arranged by size:
2425 1267 299 296
BsiHKAI G_wGCw'C
Cuts at: 0 109 385 1148 1277 1538 1838 2252 2263
Size: 109 276 763 129 261 300 414 11
Cuts at: 2263 2630 3494 3794 4287
Size: 367 864 300 493
Fragments arranged by size:
864 763 493 414 367 300 300 276
261 129 109 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2490 4287
Size: 2490 1797
Bsp1286I G_dGCh'C
Cuts at: 0 109 385 394 1148 1277 1538 1545 1559
Size: 109 276 9 754 129 261 7 14
Cuts at: 1559 1838 2252 2263 2341 2630 2837 2919 3244
Size: 279 414 11 78 289 207 82 325
Cuts at: 3244 3494 3794 4287
Size: 250 300 493
Fragments arranged by size:
754 493 414 325 300 289 279 276
261 250 207 129 109 82 78 14
11 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1026 2792 3209 4287
Size: 1026 1766 417 1078
Fragments arranged by size:
1766 1078 1026 417
BsrBI CCG'CTC
Cuts at: 0 127 1209 4287
Size: 127 1082 3078
Fragments arranged by size:
3078 1082 127
BsrDI GCAATG_nn'
Cuts at: 0 1901 4287
Size: 1901 2386
BsrFI r'CCGG_y
Cuts at: 0 372 1302 2075 3156 4287
Size: 372 930 773 1081 1131
Fragments arranged by size:
1131 1081 930 773 372
BssSI C'ACGA_G
Cuts at: 0 4128 4287
Size: 4128 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2127 2267 4002 4287
Size: 2127 140 1735 285
Fragments arranged by size:
2127 1735 285 140
BstBI TT'CG_AA
Cuts at: 0 2213 4287
Size: 2213 2074
BstEII G'GTnAC_C
Cuts at: 0 2415 3366 4287
Size: 2415 951 921
Fragments arranged by size:
2415 951 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1069 4287
Size: 1069 3218
BstYI r'GATC_y
Cuts at: 0 359 559 876 1190 3107 3927 4287
Size: 359 200 317 314 1917 820 360
Fragments arranged by size:
1917 820 360 359 317 314 200
Bsu36I CC'TnA_GG
Cuts at: 0 1771 3728 4287
Size: 1771 1957 559
Fragments arranged by size:
1957 1771 559
BtgI C'CryG_G
Cuts at: 0 697 760 2345 3245 4287
Size: 697 63 1585 900 1042
Fragments arranged by size:
1585 1042 900 697 63
BtsI GCAGTG_nn'
Cuts at: 0 350 548 1312 2070 2110 2157 2477 4287
Size: 350 198 764 758 40 47 320 1810
Fragments arranged by size:
1810 764 758 350 320 198 47 40
DraI TTT'AAA
Cuts at: 0 3561 4287
Size: 3561 726
DraIII CAC_nnn'GTG
Cuts at: 0 3507 4011 4287
Size: 3507 504 276
Fragments arranged by size:
3507 504 276
EaeI y'GGCC_r
Cuts at: 0 1028 3843 4287
Size: 1028 2815 444
Fragments arranged by size:
2815 1028 444
EarI CTCTTCn'nnn_
Cuts at: 0 114 482 1222 2404 2470 4287
Size: 114 368 740 1182 66 1817
Fragments arranged by size:
1817 1182 740 368 114 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 23 1321 4287
Size: 23 1298 2966
Fragments arranged by size:
2966 1298 23
EcoICRI GAG'CTC
Cuts at: 0 107 1146 1275 1536 2250 3792 4287
Size: 107 1039 129 261 714 1542 495
Fragments arranged by size:
1542 1039 714 495 261 129 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 82 84 462 1083 1308 2167 2284
Size: 67 15 2 378 621 225 859 117
Cuts at: 2284 2449 2536 2660 2791 2934 2941 3494 3601
Size: 165 87 124 131 143 7 553 107
Cuts at: 3601 3808 3830 3941 4231 4287
Size: 207 22 111 290 56
Fragments arranged by size:
859 621 553 378 290 225 207 165
143 131 124 117 111 107 87 67
56 22 15 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 1666 2923 4287
Size: 49 1617 1257 1364
Fragments arranged by size:
1617 1364 1257 49
EcoO109I rG'GnC_Cy
Cuts at: 0 201 608 692 1431 1542 1693 2109 2367
Size: 201 407 84 739 111 151 416 258
Cuts at: 2367 2565 2736 2915 2916 2988 3198 3240 3241
Size: 198 171 179 1 72 210 42 1
Cuts at: 3241 3250 3445 4287
Size: 9 195 842
Fragments arranged by size:
842 739 416 407 258 210 201 198
195 179 171 151 111 84 72 42
9 1 1
EcoRI G'AATT_C
Cuts at: 0 3382 4287
Size: 3382 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 143 484 516 616 648 1155 1187
Size: 111 32 341 32 100 32 507 32
Cuts at: 1187 1356 1368 1388 1400 1685 1699 1717 1731
Size: 169 12 20 12 285 14 18 14
Cuts at: 1731 2728 2760 3845 3877 4209 4241 4287
Size: 997 32 1085 32 332 32 46
Fragments arranged by size:
1085 997 507 341 332 285 169 111
100 46 32 32 32 32 32 32
32 20 18 14 14 12 12
HincII GTy'rAC
Cuts at: 0 1022 3273 4287
Size: 1022 2251 1014
Fragments arranged by size:
2251 1022 1014
HindIII A'AGCT_T
Cuts at: 0 2296 4287
Size: 2296 1991
MfeI C'AATT_G
Cuts at: 0 3116 4287
Size: 3116 1171
MscI TGG'CCA
Cuts at: 0 1030 3845 4287
Size: 1030 2815 442
Fragments arranged by size:
2815 1030 442
MslI CAynn'nnrTG
Cuts at: 0 154 343 541 3697 4287
Size: 154 189 198 3156 590
Fragments arranged by size:
3156 590 198 189 154
MspA1I CmG'CkG
Cuts at: 0 192 417 440 706 840 1065 1103 2124
Size: 192 225 23 266 134 225 38 1021
Cuts at: 2124 2304 2551 2700 2708 2940 3326 3431 3822
Size: 180 247 149 8 232 386 105 391
Cuts at: 3822 4032 4287
Size: 210 255
Fragments arranged by size:
1021 391 386 266 255 247 232 225
225 210 192 180 149 134 105 38
23 8
NaeI GCC'GGC
Cuts at: 0 374 1304 2077 4287
Size: 374 930 773 2210
Fragments arranged by size:
2210 930 773 374
NcoI C'CATG_G
Cuts at: 0 697 760 2345 4287
Size: 697 63 1585 1942
Fragments arranged by size:
1942 1585 697 63
NdeI CA'TA_TG
Cuts at: 0 1977 4287
Size: 1977 2310
NgoMIV G'CCGG_C
Cuts at: 0 372 1302 2075 4287
Size: 372 930 773 2212
Fragments arranged by size:
2212 930 773 372
NspI r_CATG'y
Cuts at: 0 1090 2229 4287
Size: 1090 1139 2058
Fragments arranged by size:
2058 1139 1090
PasI CC'CwG_GG
Cuts at: 0 387 1559 4287
Size: 387 1172 2728
Fragments arranged by size:
2728 1172 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 2739 3405 4287
Size: 2739 666 882
Fragments arranged by size:
2739 882 666
PfoI T'CCnGG_A
Cuts at: 0 100 2513 3092 3578 4287
Size: 100 2413 579 486 709
Fragments arranged by size:
2413 709 579 486 100
PmlI CAC'GTG
Cuts at: 0 2289 4287
Size: 2289 1998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 806 3776 3808 4287
Size: 774 32 2970 32 479
Fragments arranged by size:
2970 774 479 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 608 1431 1693 2109 2367 2565 2736
Size: 201 407 823 262 416 258 198 171
Cuts at: 2736 3198 4287
Size: 462 1089
Fragments arranged by size:
1089 823 462 416 407 262 258 201
198 171
PspOMI G'GGCC_C
Cuts at: 0 1541 2915 3240 4287
Size: 1541 1374 325 1047
Fragments arranged by size:
1541 1374 1047 325
PstI C_TGCA'G
Cuts at: 0 998 1108 1391 2123 2135 2472 2699 2789
Size: 998 110 283 732 12 337 227 90
Cuts at: 2789 4287
Size: 1498
Fragments arranged by size:
1498 998 732 337 283 227 110 90
12
PvuII CAG'CTG
Cuts at: 0 417 840 1065 1103 2124 2304 2551 2700
Size: 417 423 225 38 1021 180 247 149
Cuts at: 2700 2708 2940 3431 3822 4032 4287
Size: 8 232 491 391 210 255
Fragments arranged by size:
1021 491 423 417 391 255 247 232
225 210 180 149 38 8
SacI G_AGCT'C
Cuts at: 0 109 1148 1277 1538 2252 3794 4287
Size: 109 1039 129 261 714 1542 493
Fragments arranged by size:
1542 1039 714 493 261 129 109
SanDI GG'GwC_CC
Cuts at: 0 608 1693 2109 2367 4287
Size: 608 1085 416 258 1920
Fragments arranged by size:
1920 1085 608 416 258
SapI GCTCTTCn'nnn_
Cuts at: 0 114 482 1222 4287
Size: 114 368 740 3065
Fragments arranged by size:
3065 740 368 114
SexAI A'CCwGG_T
Cuts at: 0 167 4287
Size: 167 4120
SfcI C'TryA_G
Cuts at: 0 291 742 994 1104 1387 1470 1854 2119
Size: 291 451 252 110 283 83 384 265
Cuts at: 2119 2131 2468 2686 2695 2785 3308 4287
Size: 12 337 218 9 90 523 979
Fragments arranged by size:
979 523 451 384 337 291 283 265
252 218 110 90 83 12 9
SmlI C'TyrA_G
Cuts at: 0 2410 3028 3191 4161 4287
Size: 2410 618 163 970 126
Fragments arranged by size:
2410 970 618 163 126
SspI AAT'ATT
Cuts at: 0 1465 4287
Size: 1465 2822
StuI AGG'CCT
Cuts at: 0 962 3910 4287
Size: 962 2948 377
Fragments arranged by size:
2948 962 377
StyI C'CwwG_G
Cuts at: 0 697 760 1124 2345 3516 3641 3911 4287
Size: 697 63 364 1221 1171 125 270 376
Fragments arranged by size:
1221 1171 697 376 364 270 125 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 400 534 600 773 1551 2617 3397 4287
Size: 400 134 66 173 778 1066 780 890
Fragments arranged by size:
1066 890 780 778 400 173 134 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1161 1193 2952 2984 3154 3186 4168 4200
Size: 1161 32 1759 32 170 32 982 32
Cuts at: 4200 4287
Size: 87
Fragments arranged by size:
1759 1161 982 170 87 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 3252 3428 4287
Size: 543 2709 176 859
Fragments arranged by size:
2709 859 543 176
XmnI GAAnn'nnTTC
Cuts at: 0 67 4153 4287
Size: 67 4086 134
Fragments arranged by size:
4086 134 67
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanII
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HindIII MfeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SexAI SfcI SmlI SspI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AloI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BfrBI BmtI BsaBI
BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BstZ17I
BtgZI ClaI CspCI DrdI EagI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MmeI
NarI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI