Restriction map for
pF1KA0931
(Linear) (Six-base) MAPSORT of: KIAA0931.seq Check: 360 from: 1 to: 3768
KIAA0931.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 1952 3305 3768
Size: 1952 1353 463
Fragments arranged by size:
1952 1353 463
Acc65I G'GTAC_C
Cuts at: 0 532 3768
Size: 532 3236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 922 1103 1122 1714 2535 3768
Size: 295 627 181 19 592 821 1233
Fragments arranged by size:
1233 821 627 592 295 181 19
AleI CACnn'nnGTG
Cuts at: 0 1327 3577 3768
Size: 1327 2250 191
Fragments arranged by size:
2250 1327 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1218 2529 3463 3768
Size: 1218 1311 934 305
Fragments arranged by size:
1311 1218 934 305
AlwNI CAG_nnn'CTG
Cuts at: 0 2785 2953 3768
Size: 2785 168 815
Fragments arranged by size:
2785 815 168
ApaLI G'TGCA_C
Cuts at: 0 2838 3768
Size: 2838 930
ApoI r'AATT_y
Cuts at: 0 889 1121 1145 1628 3768
Size: 889 232 24 483 2140
Fragments arranged by size:
2140 889 483 232 24
AvaI C'yCGr_G
Cuts at: 0 53 781 1714 3706 3768
Size: 53 728 933 1992 62
Fragments arranged by size:
1992 933 728 62 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2317 2350 3768
Size: 2317 33 1418
Fragments arranged by size:
2317 1418 33
BamHI G'GATC_C
Cuts at: 0 2681 3410 3768
Size: 2681 729 358
Fragments arranged by size:
2681 729 358
BanI G'GyrC_C
Cuts at: 0 532 3768
Size: 532 3236
BanII G_rGCy'C
Cuts at: 0 663 980 2279 2416 2939 3719 3743 3768
Size: 663 317 1299 137 523 780 24 25
Fragments arranged by size:
1299 780 663 523 317 137 25 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 2541 3423 3768
Size: 2541 882 345
Fragments arranged by size:
2541 882 345
BbvCI CC'TCA_GC
Cuts at: 0 1414 3768
Size: 1414 2354
BclI T'GATC_A
Cuts at: 0 2100 3768
Size: 2100 1668
BglI GCCn_nnn'nGGC
Cuts at: 0 3144 3768
Size: 3144 624
BglII A'GATC_T
Cuts at: 0 959 3768
Size: 959 2809
BlpI GC'TnA_GC
Cuts at: 0 509 651 3768
Size: 509 142 3117
Fragments arranged by size:
3117 509 142
Bme1580I G_kGCm'C
Cuts at: 0 2537 2842 3539 3584 3768
Size: 2537 305 697 45 184
Fragments arranged by size:
2537 697 305 184 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 1566 3768
Size: 1566 2202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1324 2169 2578 3198 3355 3768
Size: 254 1070 845 409 620 157 413
Fragments arranged by size:
1070 845 620 413 409 254 157
Bpu10I CC'TnA_GC
Cuts at: 0 794 1414 2993 3713 3768
Size: 794 620 1579 720 55
Fragments arranged by size:
1579 794 720 620 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1662 3768
Size: 1662 2106
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 2255 3768
Size: 266 1989 1513
Fragments arranged by size:
1989 1513 266
BsaAI yAC'GTr
Cuts at: 0 1329 2498 2805 3768
Size: 1329 1169 307 963
Fragments arranged by size:
1329 1169 963 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 263 293 3768
Size: 263 30 3475
Fragments arranged by size:
3475 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 153 156 159 385 799 2287 2406
Size: 138 15 3 3 226 414 1488 119
Cuts at: 2406 2587 3763 3768
Size: 181 1176 5
Fragments arranged by size:
1488 1176 414 226 181 138 119 15
5 3 3
BseYI C'CCAG_C
Cuts at: 0 339 491 743 1501 1662 2655 3106 3768
Size: 339 152 252 758 161 993 451 662
Fragments arranged by size:
993 758 662 451 339 252 161 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1861 2901 3768
Size: 1861 1040 867
Fragments arranged by size:
1861 1040 867
BsiHKAI G_wGCw'C
Cuts at: 0 980 1706 2279 2842 3768
Size: 980 726 573 563 926
Fragments arranged by size:
980 926 726 573 563
Bsp1286I G_dGCh'C
Cuts at: 0 663 980 1706 2279 2416 2537 2842 2939
Size: 663 317 726 573 137 121 305 97
Cuts at: 2939 3539 3584 3719 3743 3768
Size: 600 45 135 24 25
Fragments arranged by size:
726 663 600 573 317 305 137 135
121 97 45 25 24
BspHI T'CATG_A
Cuts at: 0 1002 3651 3768
Size: 1002 2649 117
Fragments arranged by size:
2649 1002 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 544 1544 2647 3768
Size: 544 1000 1103 1121
Fragments arranged by size:
1121 1103 1000 544
BsrBI CCG'CTC
Cuts at: 0 2422 3144 3768
Size: 2422 722 624
Fragments arranged by size:
2422 722 624
BsrDI GCAATG_nn'
Cuts at: 0 15 3768
Size: 15 3753
BsrFI r'CCGG_y
Cuts at: 0 3701 3768
Size: 3701 67
BsrGI T'GTAC_A
Cuts at: 0 2662 3768
Size: 2662 1106
BssSI C'ACGA_G
Cuts at: 0 768 1746 3768
Size: 768 978 2022
Fragments arranged by size:
2022 978 768
BstAPI GCAn_nnn'nTGC
Cuts at: 0 588 3768
Size: 588 3180
BstEII G'GTnAC_C
Cuts at: 0 2634 3768
Size: 2634 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3621 3768
Size: 3621 147
BstYI r'GATC_y
Cuts at: 0 762 959 1332 1362 1521 1725 1940 2681
Size: 762 197 373 30 159 204 215 741
Cuts at: 2681 3330 3410 3768
Size: 649 80 358
Fragments arranged by size:
762 741 649 373 358 215 204 197
159 80 30
Bsu36I CC'TnA_GG
Cuts at: 0 1151 3768
Size: 1151 2617
BtgI C'CryG_G
Cuts at: 0 758 1286 2154 3768
Size: 758 528 868 1614
Fragments arranged by size:
1614 868 758 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 726 2896 3768
Size: 726 2170 872
Fragments arranged by size:
2170 872 726
BtsI GCAGTG_nn'
Cuts at: 0 122 1487 2299 2447 3024 3048 3075 3083
Size: 122 1365 812 148 577 24 27 8
Cuts at: 3083 3119 3312 3768
Size: 36 193 456
Fragments arranged by size:
1365 812 577 456 193 148 122 36
27 24 8
DraIII CAC_nnn'GTG
Cuts at: 0 1432 3768
Size: 1432 2336
EaeI y'GGCC_r
Cuts at: 0 698 2400 3569 3768
Size: 698 1702 1169 199
Fragments arranged by size:
1702 1169 698 199
EarI CTCTTCn'nnn_
Cuts at: 0 156 162 177 237 314 808 1771 2315
Size: 156 6 15 60 77 494 963 544
Cuts at: 2315 3719 3768
Size: 1404 49
Fragments arranged by size:
1404 963 544 494 156 77 60 49
15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1419 2428 3768
Size: 1419 1009 1340
Fragments arranged by size:
1419 1340 1009
EcoICRI GAG'CTC
Cuts at: 0 978 2277 3768
Size: 978 1299 1491
Fragments arranged by size:
1491 1299 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 295 922 1103 1122 1324 1714 2169
Size: 254 41 627 181 19 202 390 455
Cuts at: 2169 2535 2578 3198 3355 3768
Size: 366 43 620 157 413
Fragments arranged by size:
627 620 455 413 390 366 254 202
181 157 43 41 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 2560 3768
Size: 784 1776 1208
Fragments arranged by size:
1776 1208 784
EcoO109I rG'GnC_Cy
Cuts at: 0 906 2565 2950 3111 3768
Size: 906 1659 385 161 657
Fragments arranged by size:
1659 906 657 385 161
EcoRI G'AATT_C
Cuts at: 0 1628 3768
Size: 1628 2140
FspI TGC'GCA
Cuts at: 0 361 3768
Size: 361 3407
FspAI rTGC'GCAy
Cuts at: 0 361 3768
Size: 361 3407
HaeII r_GCGC'y
Cuts at: 0 516 3150 3192 3768
Size: 516 2634 42 576
Fragments arranged by size:
2634 576 516 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 316 348 1354 1386 1587 1619
Size: 263 32 21 32 1006 32 201 32
Cuts at: 1619 3768
Size: 2149
Fragments arranged by size:
2149 1006 263 201 32 32 32 32
21
HincII GTy'rAC
Cuts at: 0 2141 2336 3768
Size: 2141 195 1432
Fragments arranged by size:
2141 1432 195
HindIII A'AGCT_T
Cuts at: 0 1834 3768
Size: 1834 1934
KpnI G_GTAC'C
Cuts at: 0 536 3768
Size: 536 3232
MfeI C'AATT_G
Cuts at: 0 1804 3768
Size: 1804 1964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1490 1714 1922 3768
Size: 1490 224 208 1846
Fragments arranged by size:
1846 1490 224 208
MscI TGG'CCA
Cuts at: 0 2402 3571 3768
Size: 2402 1169 197
Fragments arranged by size:
2402 1169 197
MslI CAynn'nnrTG
Cuts at: 0 1327 1673 3565 3577 3619 3768
Size: 1327 346 1892 12 42 149
Fragments arranged by size:
1892 1327 346 149 42 12
MspA1I CmG'CkG
Cuts at: 0 495 1413 2715 2824 3768
Size: 495 918 1302 109 944
Fragments arranged by size:
1302 944 918 495 109
NaeI GCC'GGC
Cuts at: 0 3703 3768
Size: 3703 65
NcoI C'CATG_G
Cuts at: 0 1286 2154 3768
Size: 1286 868 1614
Fragments arranged by size:
1614 1286 868
NgoMIV G'CCGG_C
Cuts at: 0 3701 3768
Size: 3701 67
NspI r_CATG'y
Cuts at: 0 1258 2253 3391 3444 3768
Size: 1258 995 1138 53 324
Fragments arranged by size:
1258 1138 995 324 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 2160 2409 3768
Size: 2160 249 1359
Fragments arranged by size:
2160 1359 249
PmlI CAC'GTG
Cuts at: 0 1329 2498 3768
Size: 1329 1169 1270
Fragments arranged by size:
1329 1270 1169
PpuMI rG'GwC_Cy
Cuts at: 0 2565 2950 3768
Size: 2565 385 818
Fragments arranged by size:
2565 818 385
PspXI vC'TCGA_Gb
Cuts at: 0 781 1714 3706 3768
Size: 781 933 1992 62
Fragments arranged by size:
1992 933 781 62
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 33 65 3768
Size: 33 32 3703
Fragments arranged by size:
3703 33 32
PstI C_TGCA'G
Cuts at: 0 1931 2294 3154 3322 3768
Size: 1931 363 860 168 446
Fragments arranged by size:
1931 860 446 363 168
PvuII CAG'CTG
Cuts at: 0 495 1413 2824 3768
Size: 495 918 1411 944
Fragments arranged by size:
1411 944 918 495
RsrII CG'GwC_CG
Cuts at: 0 3734 3768
Size: 3734 34
SacI G_AGCT'C
Cuts at: 0 980 2279 3768
Size: 980 1299 1489
Fragments arranged by size:
1489 1299 980
SexAI A'CCwGG_T
Cuts at: 0 275 3768
Size: 275 3493
SfcI C'TryA_G
Cuts at: 0 203 807 1927 2129 2290 2480 3150 3318
Size: 203 604 1120 202 161 190 670 168
Cuts at: 3318 3768
Size: 450
Fragments arranged by size:
1120 670 604 450 203 202 190 168
161
SmlI C'TyrA_G
Cuts at: 0 53 781 1641 1714 3706 3768
Size: 53 728 860 73 1992 62
Fragments arranged by size:
1992 860 728 73 62 53
SphI G_CATG'C
Cuts at: 0 3444 3768
Size: 3444 324
SspI AAT'ATT
Cuts at: 0 2901 3768
Size: 2901 867
StuI AGG'CCT
Cuts at: 0 872 1777 3768
Size: 872 905 1991
Fragments arranged by size:
1991 905 872
StyI C'CwwG_G
Cuts at: 0 731 1115 1286 1772 2154 2677 3768
Size: 731 384 171 486 382 523 1091
Fragments arranged by size:
1091 731 523 486 384 382 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1339 2279 3176 3183 3751 3768
Size: 1339 940 897 7 568 17
Fragments arranged by size:
1339 940 897 568 17 7
TatI w'GTAC_w
Cuts at: 0 2662 3768
Size: 2662 1106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1602 1634 2439 2471 2689 2721
Size: 754 32 816 32 805 32 218 32
Cuts at: 2721 3768
Size: 1047
Fragments arranged by size:
1047 816 805 754 218 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 277 3768
Size: 277 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 928 3768
Size: 928 2840
XhoI C'TCGA_G
Cuts at: 0 53 781 1714 3706 3768
Size: 53 728 933 1992 62
Fragments arranged by size:
1992 933 728 62 53
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BclI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PmlI PpuMI PspXI PsrI PstI PvuII RsrII
SacI SexAI SfcI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BbeI BcgI
BciVI BfrBI BmgBI BmtI BplI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BssHII BstBI BstZ17I
ClaI CspCI DraI DrdI EagI EcoRV FalI FseI
HpaI KasI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PvuI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI XbaI XmaI XmnI ZraI