Restriction map for
pF1KA0933
(Linear) (Six-base) MAPSORT of: KIAA0933.seq Check: 3153 from: 1 to: 6741
KIAA0933.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2273 3014 4173 4657 6741
Size: 2273 741 1159 484 2084
Fragments arranged by size:
2273 2084 1159 741 484
AccI GT'mk_AC
Cuts at: 0 2294 5006 6741
Size: 2294 2712 1735
Fragments arranged by size:
2712 2294 1735
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 127 1102 1771 2401 3017 4192 5477
Size: 38 89 975 669 630 616 1175 1285
Cuts at: 5477 5943 6741
Size: 466 798
Fragments arranged by size:
1285 1175 975 798 669 630 616 466
89 38
AfeI AGC'GCT
Cuts at: 0 3319 4048 6323 6741
Size: 3319 729 2275 418
Fragments arranged by size:
3319 2275 729 418
AflII C'TTAA_G
Cuts at: 0 1557 6741
Size: 1557 5184
AflIII A'CryG_T
Cuts at: 0 866 1591 3218 3758 6741
Size: 866 725 1627 540 2983
Fragments arranged by size:
2983 1627 866 725 540
AhdI GACnn_n'nnGTC
Cuts at: 0 969 1644 4613 6741
Size: 969 675 2969 2128
Fragments arranged by size:
2969 2128 969 675
AleI CACnn'nnGTG
Cuts at: 0 3028 6741
Size: 3028 3713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1553 2342 4099 6741
Size: 1553 789 1757 2642
Fragments arranged by size:
2642 1757 1553 789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1650 1682 2828 2860 3208 3240 6741
Size: 1650 32 1146 32 348 32 3501
Fragments arranged by size:
3501 1650 1146 348 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 414 940 2010 2085 2478 3258 4188 4915
Size: 414 526 1070 75 393 780 930 727
Cuts at: 4915 5709 6741
Size: 794 1032
Fragments arranged by size:
1070 1032 930 794 780 727 526 414
393 75
ApaI G_GGCC'C
Cuts at: 0 4083 4221 6403 6741
Size: 4083 138 2182 338
Fragments arranged by size:
4083 2182 338 138
ApaLI G'TGCA_C
Cuts at: 0 3281 5018 6741
Size: 3281 1737 1723
Fragments arranged by size:
3281 1737 1723
ApoI r'AATT_y
Cuts at: 0 78 951 1146 1236 1296 3616 4859 6307
Size: 78 873 195 90 60 2320 1243 1448
Cuts at: 6307 6359 6461 6529 6741
Size: 52 102 68 212
Fragments arranged by size:
2320 1448 1243 873 212 195 102 90
78 68 60 52
AvaI C'yCGr_G
Cuts at: 0 90 1662 1820 2812 2974 3070 4253 6741
Size: 90 1572 158 992 162 96 1183 2488
Fragments arranged by size:
2488 1572 1183 992 162 158 96 90
AvrII C'CTAG_G
Cuts at: 0 4797 6741
Size: 4797 1944
BamHI G'GATC_C
Cuts at: 0 3 4813 6741
Size: 3 4810 1928
Fragments arranged by size:
4810 1928 3
BanI G'GyrC_C
Cuts at: 0 2764 3151 6741
Size: 2764 387 3590
Fragments arranged by size:
3590 2764 387
BanII G_rGCy'C
Cuts at: 0 23 475 1433 1778 1799 2051 2521 3071
Size: 23 452 958 345 21 252 470 550
Cuts at: 3071 3452 4083 4221 4272 5716 6403 6741
Size: 381 631 138 51 1444 687 338
Fragments arranged by size:
1444 958 687 631 550 470 452 381
345 338 252 138 51 23 21
BbeI G_GCGC'C
Cuts at: 0 3155 6741
Size: 3155 3586
BbsI GAAGACnn'nnnn_
Cuts at: 0 594 888 968 1440 1594 2141 2501 3932
Size: 594 294 80 472 154 547 360 1431
Cuts at: 3932 4330 5498 5949 6741
Size: 398 1168 451 792
Fragments arranged by size:
1431 1168 792 594 547 472 451 398
360 294 154 80
BbvCI CC'TCA_GC
Cuts at: 0 1541 5462 6741
Size: 1541 3921 1279
Fragments arranged by size:
3921 1541 1279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1957 1991 6741
Size: 1957 34 4750
Fragments arranged by size:
4750 1957 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3642 5793 6741
Size: 3642 2151 948
Fragments arranged by size:
3642 2151 948
BclI T'GATC_A
Cuts at: 0 3902 5973 6741
Size: 3902 2071 768
Fragments arranged by size:
3902 2071 768
BfrBI ATG'CAT
Cuts at: 0 892 1075 4392 6707 6741
Size: 892 183 3317 2315 34
Fragments arranged by size:
3317 2315 892 183 34
BglI GCCn_nnn'nGGC
Cuts at: 0 177 3049 3331 3511 6741
Size: 177 2872 282 180 3230
Fragments arranged by size:
3230 2872 282 180 177
BglII A'GATC_T
Cuts at: 0 479 811 4136 6171 6741
Size: 479 332 3325 2035 570
Fragments arranged by size:
3325 2035 570 479 332
BlpI GC'TnA_GC
Cuts at: 0 1606 3097 3259 5404 5702 5710 6741
Size: 1606 1491 162 2145 298 8 1031
Fragments arranged by size:
2145 1606 1491 1031 298 162 8
Bme1580I G_kGCm'C
Cuts at: 0 2975 3285 3766 4083 4221 5022 6403 6741
Size: 2975 310 481 317 138 801 1381 338
Fragments arranged by size:
2975 1381 801 481 338 317 310 138
BmgBI CAC'GTC
Cuts at: 0 1056 1592 2820 3221 3876 6741
Size: 1056 536 1228 401 655 2865
Fragments arranged by size:
2865 1228 1056 655 536 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 419 1491 2466 5161 6741
Size: 221 198 1072 975 2695 1580
Fragments arranged by size:
2695 1580 1072 975 221 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1835 3009 3438 3877 6741
Size: 1835 1174 429 439 2864
Fragments arranged by size:
2864 1835 1174 439 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2410 2561 3408 3436 3607 3724 3802 4161
Size: 2410 151 847 28 171 117 78 359
Cuts at: 4161 4556 5769 5785 6741
Size: 395 1213 16 956
Fragments arranged by size:
2410 1213 956 847 395 359 171 151
117 78 28 16
Bpu10I CC'TnA_GC
Cuts at: 0 1541 3805 5462 6741
Size: 1541 2264 1657 1279
Fragments arranged by size:
2264 1657 1541 1279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1830 2878 4704 5892 6741
Size: 1118 712 1048 1826 1188 849
Fragments arranged by size:
1826 1188 1118 1048 849 712
BsaI GGTCTCn'nnnn_
Cuts at: 0 1428 2117 2305 3849 3930 4350 4776 6138
Size: 1428 689 188 1544 81 420 426 1362
Cuts at: 6138 6741
Size: 603
Fragments arranged by size:
1544 1428 1362 689 603 426 420 188
81
BsaAI yAC'GTr
Cuts at: 0 3123 4308 6741
Size: 3123 1185 2433
Fragments arranged by size:
3123 2433 1185
BsaBI GATnn'nnATC
Cuts at: 0 2615 6741
Size: 2615 4126
BsaHI Gr'CG_yC
Cuts at: 0 3152 4024 6741
Size: 3152 872 2717
Fragments arranged by size:
3152 2717 872
BsaWI w'CCGG_w
Cuts at: 0 1791 6741
Size: 1791 4950
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 2550 2580 2695 2725 2828 2858
Size: 634 30 1886 30 115 30 103 30
Cuts at: 2858 3208 3238 3664 3694 5277 5307 5458 5488
Size: 350 30 426 30 1583 30 151 30
Cuts at: 5488 6741
Size: 1253
Fragments arranged by size:
1886 1583 1253 634 426 350 151 115
103 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 796 1984 3222 3448 3679 4120 4240
Size: 172 624 1188 1238 226 231 441 120
Cuts at: 4240 4783 5479 6089 6439 6741
Size: 543 696 610 350 302
Fragments arranged by size:
1238 1188 696 624 610 543 441 350
302 231 226 172 120
BseYI C'CCAG_C
Cuts at: 0 657 2155 3361 4946 4991 5683 6741
Size: 657 1498 1206 1585 45 692 1058
Fragments arranged by size:
1585 1498 1206 1058 692 657 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1549 2739 3898 4330 5035 6741
Size: 1549 1190 1159 432 705 1706
Fragments arranged by size:
1706 1549 1190 1159 705 432
BsiEI CG_ry'CG
Cuts at: 0 639 2912 6741
Size: 639 2273 3829
Fragments arranged by size:
3829 2273 639
BsiHKAI G_wGCw'C
Cuts at: 0 23 1433 1517 1607 1799 1808 2521 2846
Size: 23 1410 84 90 192 9 713 325
Cuts at: 2846 3005 3173 3285 3452 3599 4151 5022 5716
Size: 159 168 112 167 147 552 871 694
Cuts at: 5716 6741
Size: 1025
Fragments arranged by size:
1410 1025 871 713 694 552 325 192
168 167 159 147 112 90 84 23
9
BsmI GAATG_Cn'
Cuts at: 0 827 1366 4419 4695 6445 6709 6741
Size: 827 539 3053 276 1750 264 32
Fragments arranged by size:
3053 1750 827 539 276 264 32
Bsp1286I G_dGCh'C
Cuts at: 0 23 475 1433 1517 1607 1778 1799 1808
Size: 23 452 958 84 90 171 21 9
Cuts at: 1808 2051 2521 2846 2975 3005 3071 3173 3285
Size: 243 470 325 129 30 66 102 112
Cuts at: 3285 3452 3599 3766 4083 4151 4221 4272 5022
Size: 167 147 167 317 68 70 51 750
Cuts at: 5022 5716 6403 6741
Size: 694 687 338
Fragments arranged by size:
958 750 694 687 470 452 338 325
317 243 171 167 167 147 129 112
102 90 84 70 68 66 51 30
23 21 9
BspHI T'CATG_A
Cuts at: 0 2390 5847 6587 6741
Size: 2390 3457 740 154
Fragments arranged by size:
3457 2390 740 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2273 3014 3257 3548 3870 4173 4657 5336
Size: 2273 741 243 291 322 303 484 679
Cuts at: 5336 6741
Size: 1405
Fragments arranged by size:
2273 1405 741 679 484 322 303 291
243
BsrBI CCG'CTC
Cuts at: 0 2990 6741
Size: 2990 3751
BsrDI GCAATG_nn'
Cuts at: 0 2570 2594 5369 5504 6741
Size: 2570 24 2775 135 1237
Fragments arranged by size:
2775 2570 1237 135 24
BsrFI r'CCGG_y
Cuts at: 0 366 2353 5374 6741
Size: 366 1987 3021 1367
Fragments arranged by size:
3021 1987 1367 366
BsrGI T'GTAC_A
Cuts at: 0 314 4031 5223 5525 6741
Size: 314 3717 1192 302 1216
Fragments arranged by size:
3717 1216 1192 314 302
BssSI C'ACGA_G
Cuts at: 0 2105 4035 6741
Size: 2105 1930 2706
Fragments arranged by size:
2706 2105 1930
BstAPI GCAn_nnn'nTGC
Cuts at: 0 414 3531 4155 4308 4425 6576 6741
Size: 414 3117 624 153 117 2151 165
Fragments arranged by size:
3117 2151 624 414 165 153 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 2451 3294 6741
Size: 294 2157 843 3447
Fragments arranged by size:
3447 2157 843 294
BstYI r'GATC_y
Cuts at: 0 3 105 479 811 999 2678 4136 4813
Size: 3 102 374 332 188 1679 1458 677
Cuts at: 4813 6171 6683 6741
Size: 1358 512 58
Fragments arranged by size:
1679 1458 1358 677 512 374 332 188
102 58 3
Bsu36I CC'TnA_GG
Cuts at: 0 154 1508 2981 3611 5435 6741
Size: 154 1354 1473 630 1824 1306
Fragments arranged by size:
1824 1473 1354 1306 630 154
BtgI C'CryG_G
Cuts at: 0 1418 3147 3293 4071 4658 4736 6741
Size: 1418 1729 146 778 587 78 2005
Fragments arranged by size:
2005 1729 1418 778 587 146 78
BtsI GCAGTG_nn'
Cuts at: 0 403 1718 2886 2975 4931 6741
Size: 403 1315 1168 89 1956 1810
Fragments arranged by size:
1956 1810 1315 1168 403 89
DraI TTT'AAA
Cuts at: 0 273 4833 6741
Size: 273 4560 1908
Fragments arranged by size:
4560 1908 273
DraIII CAC_nnn'GTG
Cuts at: 0 5058 6741
Size: 5058 1683
DrdI GACnn_nn'nnGTC
Cuts at: 0 3880 6664 6741
Size: 3880 2784 77
Fragments arranged by size:
3880 2784 77
EaeI y'GGCC_r
Cuts at: 0 296 569 636 2435 2909 3198 3717 3932
Size: 296 273 67 1799 474 289 519 215
Cuts at: 3932 4058 6121 6741
Size: 126 2063 620
Fragments arranged by size:
2063 1799 620 519 474 296 289 273
215 126 67
EagI C'GGCC_G
Cuts at: 0 636 2909 6741
Size: 636 2273 3832
Fragments arranged by size:
3832 2273 636
EarI CTCTTCn'nnn_
Cuts at: 0 5 57 146 461 1978 2303 2341 2993
Size: 5 52 89 315 1517 325 38 652
Cuts at: 2993 3101 3263 4706 4824 5068 5385 6517 6741
Size: 108 162 1443 118 244 317 1132 224
Fragments arranged by size:
1517 1443 1132 652 325 317 315 244
224 162 118 108 89 52 38 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 574 3454 4246 5502 6741
Size: 118 456 2880 792 1256 1239
Fragments arranged by size:
2880 1256 1239 792 456 118
EcoICRI GAG'CTC
Cuts at: 0 21 1431 1797 2519 3450 5714 6741
Size: 21 1410 366 722 931 2264 1027
Fragments arranged by size:
2264 1410 1027 931 722 366 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 127 1102 1771 2401 2410 2561 3017
Size: 38 89 975 669 630 9 151 456
Cuts at: 3017 3408 3436 3607 3724 3802 4161 4192 4556
Size: 391 28 171 117 78 359 31 364
Cuts at: 4556 5477 5769 5785 5943 6741
Size: 921 292 16 158 798
Fragments arranged by size:
975 921 798 669 630 456 391 364
359 292 171 158 151 117 89 78
38 31 28 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 2302 2718 2896 4087 4102 4554 5059
Size: 466 1836 416 178 1191 15 452 505
Cuts at: 5059 5554 6741
Size: 495 1187
Fragments arranged by size:
1836 1191 1187 505 495 466 452 416
178 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1129 1169 2723 3456 3564 3708 3750 4079
Size: 1129 40 1554 733 108 144 42 329
Cuts at: 4079 4080 5469 5760 6399 6400 6741
Size: 1 1389 291 639 1 341
Fragments arranged by size:
1554 1389 1129 733 639 341 329 291
144 108 42 40 1 1
EcoRI G'AATT_C
Cuts at: 0 3616 6461 6529 6741
Size: 3616 2845 68 212
Fragments arranged by size:
3616 2845 212 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 68 6741
Size: 68 6673
FspI TGC'GCA
Cuts at: 0 826 2967 6741
Size: 826 2141 3774
Fragments arranged by size:
3774 2141 826
FspAI rTGC'GCAy
Cuts at: 0 826 6741
Size: 826 5915
HaeII r_GCGC'y
Cuts at: 0 2485 3155 3321 4050 4298 5702 5943 6325
Size: 2485 670 166 729 248 1404 241 382
Cuts at: 6325 6741
Size: 416
Fragments arranged by size:
2485 1404 729 670 416 382 248 241
166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 167 199 803 835 955 987
Size: 97 32 38 32 604 32 120 32
Cuts at: 987 1017 1049 1296 1328 1630 1662 2128 2160
Size: 30 32 247 32 302 32 466 32
Cuts at: 2160 2550 2582 2649 2681 3178 3205 3210 3237
Size: 390 32 67 32 497 27 5 27
Cuts at: 3237 3616 3640 3648 3672 4599 4631 4875 4907
Size: 379 24 8 24 927 32 244 32
Cuts at: 4907 5092 5124 6006 6038 6741
Size: 185 32 882 32 703
Fragments arranged by size:
927 882 703 604 497 466 390 379
302 247 244 185 120 97 67 38
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 27
27 24 24 8 5
HincII GTy'rAC
Cuts at: 0 4731 4907 5007 6741
Size: 4731 176 100 1734
Fragments arranged by size:
4731 1734 176 100
HindIII A'AGCT_T
Cuts at: 0 3351 6741
Size: 3351 3390
HpaI GTT'AAC
Cuts at: 0 4731 6741
Size: 4731 2010
KasI G'GCGC_C
Cuts at: 0 3151 6741
Size: 3151 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 686 3707 6012 6395 6741
Size: 509 177 3021 2305 383 346
Fragments arranged by size:
3021 2305 509 383 346 177
MscI TGG'CCA
Cuts at: 0 298 571 2437 3719 3934 4060 6123 6741
Size: 298 273 1866 1282 215 126 2063 618
Fragments arranged by size:
2063 1866 1282 618 298 273 215 126
MslI CAynn'nnrTG
Cuts at: 0 2062 3028 3292 5128 6741
Size: 2062 966 264 1836 1613
Fragments arranged by size:
2062 1836 1613 966 264
MspA1I CmG'CkG
Cuts at: 0 320 1655 1938 2343 3084 3489 3921 4155
Size: 320 1335 283 405 741 405 432 234
Cuts at: 4155 4912 5399 5706 6624 6741
Size: 757 487 307 918 117
Fragments arranged by size:
1335 918 757 741 487 432 405 405
320 307 283 234 117
NaeI GCC'GGC
Cuts at: 0 5376 6741
Size: 5376 1365
NarI GG'CG_CC
Cuts at: 0 3152 6741
Size: 3152 3589
NcoI C'CATG_G
Cuts at: 0 3293 4658 4736 6741
Size: 3293 1365 78 2005
Fragments arranged by size:
3293 2005 1365 78
NgoMIV G'CCGG_C
Cuts at: 0 5374 6741
Size: 5374 1367
NsiI A_TGCA'T
Cuts at: 0 894 1077 4394 6709 6741
Size: 894 183 3317 2315 32
Fragments arranged by size:
3317 2315 894 183 32
NspI r_CATG'y
Cuts at: 0 657 870 1582 1869 2966 4392 5271 5883
Size: 657 213 712 287 1097 1426 879 612
Cuts at: 5883 6741
Size: 858
Fragments arranged by size:
1426 1097 879 858 712 657 612 287
213
PasI CC'CwG_GG
Cuts at: 0 2784 4332 4446 6741
Size: 2784 1548 114 2295
Fragments arranged by size:
2784 2295 1548 114
PciI A'CATG_T
Cuts at: 0 866 6741
Size: 866 5875
PflMI CCAn_nnn'nTGG
Cuts at: 0 2433 2478 6741
Size: 2433 45 4263
Fragments arranged by size:
4263 2433 45
PfoI T'CCnGG_A
Cuts at: 0 3634 3779 5573 5762 6236 6741
Size: 3634 145 1794 189 474 505
Fragments arranged by size:
3634 1794 505 474 189 145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 2828 2860 3208 3240 3502 3534
Size: 483 32 2313 32 348 32 262 32
Cuts at: 3534 6741
Size: 3207
Fragments arranged by size:
3207 2313 483 348 262 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 2723 3564 3750 5469 5760 6741
Size: 2723 841 186 1719 291 981
Fragments arranged by size:
2723 1719 981 841 291 186
PshAI GACnn'nnGTC
Cuts at: 0 3748 6741
Size: 3748 2993
PspOMI G'GGCC_C
Cuts at: 0 4079 4217 6399 6741
Size: 4079 138 2182 342
Fragments arranged by size:
4079 2182 342 138
PspXI vC'TCGA_Gb
Cuts at: 0 90 1662 1820 6741
Size: 90 1572 158 4921
Fragments arranged by size:
4921 1572 158 90
PstI C_TGCA'G
Cuts at: 0 410 434 1189 1980 2552 2861 3186 3254
Size: 410 24 755 791 572 309 325 68
Cuts at: 3254 3545 3983 4160 4184 4304 4370 4668 5036
Size: 291 438 177 24 120 66 298 368
Cuts at: 5036 5333 5490 6741
Size: 297 157 1251
Fragments arranged by size:
1251 791 755 572 438 410 368 325
309 298 297 291 177 157 120 68
66 24 24
PvuII CAG'CTG
Cuts at: 0 320 1938 2343 3921 5399 5706 6624 6741
Size: 320 1618 405 1578 1478 307 918 117
Fragments arranged by size:
1618 1578 1478 918 405 320 307 117
SacI G_AGCT'C
Cuts at: 0 23 1433 1799 2521 3452 5716 6741
Size: 23 1410 366 722 931 2264 1025
Fragments arranged by size:
2264 1410 1025 931 722 366 23
SalI G'TCGA_C
Cuts at: 0 5005 6741
Size: 5005 1736
SapI GCTCTTCn'nnn_
Cuts at: 0 5 146 2341 2993 3101 4706 5385 6741
Size: 5 141 2195 652 108 1605 679 1356
Fragments arranged by size:
2195 1605 1356 679 652 141 108 5
SbfI CC_TGCA'GG
Cuts at: 0 1980 3254 5333 6741
Size: 1980 1274 2079 1408
Fragments arranged by size:
2079 1980 1408 1274
ScaI AGT'ACT
Cuts at: 0 394 6741
Size: 394 6347
SexAI A'CCwGG_T
Cuts at: 0 5054 6741
Size: 5054 1687
SfcI C'TryA_G
Cuts at: 0 406 430 1185 1976 2548 2857 3182 3250
Size: 406 24 755 791 572 309 325 68
Cuts at: 3250 3541 3979 4156 4180 4300 4366 4664 5032
Size: 291 438 177 24 120 66 298 368
Cuts at: 5032 5329 5486 6741
Size: 297 157 1255
Fragments arranged by size:
1255 791 755 572 438 406 368 325
309 298 297 291 177 157 120 68
66 24 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3511 6741
Size: 3511 3230
SfoI GGC'GCC
Cuts at: 0 3153 6741
Size: 3153 3588
SmaI CCC'GGG
Cuts at: 0 4255 6741
Size: 4255 2486
SmlI C'TyrA_G
Cuts at: 0 90 1133 1557 1662 1809 1820 2893 4683
Size: 90 1043 424 105 147 11 1073 1790
Cuts at: 4683 5907 6741
Size: 1224 834
Fragments arranged by size:
1790 1224 1073 1043 834 424 147 105
90 11
SphI G_CATG'C
Cuts at: 0 1582 1869 2966 4392 5271 6741
Size: 1582 287 1097 1426 879 1470
Fragments arranged by size:
1582 1470 1426 1097 879 287
StuI AGG'CCT
Cuts at: 0 436 1555 2245 3514 5440 6741
Size: 436 1119 690 1269 1926 1301
Fragments arranged by size:
1926 1301 1269 1119 690 436
StyI C'CwwG_G
Cuts at: 0 299 995 1197 2682 3293 4658 4736 4797
Size: 299 696 202 1485 611 1365 78 61
Cuts at: 4797 5384 6679 6741
Size: 587 1295 62
Fragments arranged by size:
1485 1365 1295 696 611 587 299 202
78 62 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 859 1240 2329 3300 4392 6741
Size: 859 381 1089 971 1092 2349
Fragments arranged by size:
2349 1092 1089 971 859 381
TatI w'GTAC_w
Cuts at: 0 314 392 650 1932 4031 5223 5525 6741
Size: 314 78 258 1282 2099 1192 302 1216
Fragments arranged by size:
2099 1282 1216 1192 314 302 258 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2695 2727 6242 6274 6741
Size: 2695 32 3515 32 467
Fragments arranged by size:
3515 2695 467 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1944 4501 6741
Size: 1944 2557 2240
Fragments arranged by size:
2557 2240 1944
XbaI T'CTAG_A
Cuts at: 0 4593 5445 6465 6741
Size: 4593 852 1020 276
Fragments arranged by size:
4593 1020 852 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3375 3642 6741
Size: 3375 267 3099
Fragments arranged by size:
3375 3099 267
XhoI C'TCGA_G
Cuts at: 0 90 1662 1820 6741
Size: 90 1572 158 4921
Fragments arranged by size:
4921 1572 158 90
XmaI C'CCGG_G
Cuts at: 0 4253 6741
Size: 4253 2488
XmnI GAAnn'nnTTC
Cuts at: 0 394 746 5902 6143 6741
Size: 394 352 5156 241 598
Fragments arranged by size:
5156 598 394 352 241
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AscI AseI AsiSI BaeI
BmtI BsiWI BsmBI BspEI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI EcoRV FseI KpnI MfeI MluI
NdeI NheI NotI NruI PacI PmeI PmlI PsiI
PsrI PvuI RsrII SacII SanDI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI ZraI