Restriction map for
pF1KSDA0936
(Linear) (Six-base) MAPSORT of: KIAA0936.seq Check: 4495 from: 1 to: 1896
KIAA0936.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 972 1896
Size: 972 924
AatII G_ACGT'C
Cuts at: 0 548 1414 1896
Size: 548 866 482
Fragments arranged by size:
866 548 482
AccI GT'mk_AC
Cuts at: 0 520 1896
Size: 520 1376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 778 874 1384 1568 1777 1896
Size: 695 83 96 510 184 209 119
Fragments arranged by size:
695 510 209 184 119 96 83
AlwNI CAG_nnn'CTG
Cuts at: 0 780 1896
Size: 780 1116
ApoI r'AATT_y
Cuts at: 0 112 632 995 1534 1614 1896
Size: 112 520 363 539 80 282
Fragments arranged by size:
539 520 363 282 112 80
AvaI C'yCGr_G
Cuts at: 0 28 440 1896
Size: 28 412 1456
Fragments arranged by size:
1456 412 28
BamHI G'GATC_C
Cuts at: 0 801 1896
Size: 801 1095
BanII G_rGCy'C
Cuts at: 0 501 1896
Size: 501 1395
BbvCI CC'TCA_GC
Cuts at: 0 1288 1896
Size: 1288 608
BclI T'GATC_A
Cuts at: 0 216 1896
Size: 216 1680
BfrBI ATG'CAT
Cuts at: 0 134 1896
Size: 134 1762
BglII A'GATC_T
Cuts at: 0 1444 1896
Size: 1444 452
Bme1580I G_kGCm'C
Cuts at: 0 1387 1401 1896
Size: 1387 14 495
Fragments arranged by size:
1387 495 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 1878 1896
Size: 1878 18
BmtI G_CTAG'C
Cuts at: 0 761 1896
Size: 761 1135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 623 1050 1075 1189 1820 1896
Size: 485 138 427 25 114 631 76
Fragments arranged by size:
631 485 427 138 114 76 25
Bpu10I CC'TnA_GC
Cuts at: 0 1288 1896
Size: 1288 608
BsaI GGTCTCn'nnnn_
Cuts at: 0 1068 1761 1896
Size: 1068 693 135
Fragments arranged by size:
1068 693 135
BsaAI yAC'GTr
Cuts at: 0 1043 1896
Size: 1043 853
BsaHI Gr'CG_yC
Cuts at: 0 545 1411 1896
Size: 545 866 485
Fragments arranged by size:
866 545 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 517 547 1896
Size: 139 30 348 30 1349
Fragments arranged by size:
1349 348 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 1896
Size: 382 1514
BseYI C'CCAG_C
Cuts at: 0 57 645 968 1799 1844 1896
Size: 57 588 323 831 45 52
Fragments arranged by size:
831 588 323 57 52 45
BsiHKAI G_wGCw'C
Cuts at: 0 1175 1896
Size: 1175 721
BsmI GAATG_Cn'
Cuts at: 0 136 1896
Size: 136 1760
Bsp1286I G_dGCh'C
Cuts at: 0 501 1175 1387 1401 1896
Size: 501 674 212 14 495
Fragments arranged by size:
674 501 495 212 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 972 1331 1896
Size: 972 359 565
Fragments arranged by size:
972 565 359
BsrDI GCAATG_nn'
Cuts at: 0 704 1896
Size: 704 1192
BstYI r'GATC_y
Cuts at: 0 801 1444 1896
Size: 801 643 452
Fragments arranged by size:
801 643 452
Bsu36I CC'TnA_GG
Cuts at: 0 514 586 1896
Size: 514 72 1310
Fragments arranged by size:
1310 514 72
BtgI C'CryG_G
Cuts at: 0 1552 1828 1896
Size: 1552 276 68
Fragments arranged by size:
1552 276 68
BtsI GCAGTG_nn'
Cuts at: 0 768 1896
Size: 768 1128
DraI TTT'AAA
Cuts at: 0 161 1896
Size: 161 1735
DraIII CAC_nnn'GTG
Cuts at: 0 721 1896
Size: 721 1175
EaeI y'GGCC_r
Cuts at: 0 712 1857 1896
Size: 712 1145 39
Fragments arranged by size:
1145 712 39
EarI CTCTTCn'nnn_
Cuts at: 0 601 1896
Size: 601 1295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 623 695 778 874 1050 1075 1189
Size: 485 138 72 83 96 176 25 114
Cuts at: 1189 1384 1568 1777 1820 1896
Size: 195 184 209 43 76
Fragments arranged by size:
485 209 195 184 176 138 114 96
83 76 72 43 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 925 1896
Size: 145 780 971
Fragments arranged by size:
971 780 145
EcoRV GAT'ATC
Cuts at: 0 841 1896
Size: 841 1055
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 463 495 1401 1433 1758 1790
Size: 139 32 292 32 906 32 325 32
Cuts at: 1790 1896
Size: 106
Fragments arranged by size:
906 325 292 139 106 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1232 1896
Size: 1232 664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 1896
Size: 837 1059
MscI TGG'CCA
Cuts at: 0 714 1896
Size: 714 1182
MslI CAynn'nnrTG
Cuts at: 0 179 1178 1896
Size: 179 999 718
Fragments arranged by size:
999 718 179
MspA1I CmG'CkG
Cuts at: 0 1162 1422 1896
Size: 1162 260 474
Fragments arranged by size:
1162 474 260
NcoI C'CATG_G
Cuts at: 0 1552 1896
Size: 1552 344
NheI G'CTAG_C
Cuts at: 0 757 1896
Size: 757 1139
NsiI A_TGCA'T
Cuts at: 0 136 1896
Size: 136 1760
NspI r_CATG'y
Cuts at: 0 792 1896
Size: 792 1104
PflMI CCAn_nnn'nTGG
Cuts at: 0 645 1855 1896
Size: 645 1210 41
Fragments arranged by size:
1210 645 41
PfoI T'CCnGG_A
Cuts at: 0 920 1066 1091 1896
Size: 920 146 25 805
Fragments arranged by size:
920 805 146 25
PstI C_TGCA'G
Cuts at: 0 1342 1452 1896
Size: 1342 110 444
Fragments arranged by size:
1342 444 110
PvuII CAG'CTG
Cuts at: 0 1162 1896
Size: 1162 734
ScaI AGT'ACT
Cuts at: 0 509 1896
Size: 509 1387
SfcI C'TryA_G
Cuts at: 0 528 1338 1448 1896
Size: 528 810 110 448
Fragments arranged by size:
810 528 448 110
SspI AAT'ATT
Cuts at: 0 626 1896
Size: 626 1270
StyI C'CwwG_G
Cuts at: 0 1552 1896
Size: 1552 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 878 1094 1420 1813 1896
Size: 878 216 326 393 83
Fragments arranged by size:
878 393 326 216 83
TatI w'GTAC_w
Cuts at: 0 239 507 1896
Size: 239 268 1389
Fragments arranged by size:
1389 268 239
Tth111I GACn'n_nGTC
Cuts at: 0 1593 1896
Size: 1593 303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1550 1852 1896
Size: 1550 302 44
Fragments arranged by size:
1550 302 44
XmnI GAAnn'nnTTC
Cuts at: 0 999 1538 1581 1896
Size: 999 539 43 315
Fragments arranged by size:
999 539 315 43
ZraI GAC'GTC
Cuts at: 0 546 1412 1896
Size: 546 866 484
Fragments arranged by size:
866 546 484
Enzymes that do cut:
AarI AatII AccI AcuI AlwNI ApoI AvaI BamHI
BanII BbvCI BclI BfrBI BglII Bme1580I BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BstYI Bsu36I BtgI
BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoRV
Hin4I HincII MmeI MscI MslI MspA1I NcoI NheI
NsiI NspI PflMI PfoI PstI PvuII ScaI SfcI
SspI StyI TaqII TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BglI BlpI
BmgBI BplI BpuEI BsaBI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TstI XbaI XhoI XmaI