Restriction map for
pF1KA0938
(Linear) (Six-base) MAPSORT of: KIAA0938.seq Check: 134 from: 1 to: 5526
KIAA0938.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2257 5526
Size: 2257 3269
AatII G_ACGT'C
Cuts at: 0 4248 5526
Size: 4248 1278
AccI GT'mk_AC
Cuts at: 0 705 841 5526
Size: 705 136 4685
Fragments arranged by size:
4685 705 136
Acc65I G'GTAC_C
Cuts at: 0 799 5526
Size: 799 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 383 434 736 1427 1598 2797 3164
Size: 49 334 51 302 691 171 1199 367
Cuts at: 3164 3388 3713 3799 5185 5290 5526
Size: 224 325 86 1386 105 236
Fragments arranged by size:
1386 1199 691 367 334 325 302 236
224 171 105 86 51 49
AflII C'TTAA_G
Cuts at: 0 2472 5526
Size: 2472 3054
AflIII A'CryG_T
Cuts at: 0 374 884 929 5526
Size: 374 510 45 4597
Fragments arranged by size:
4597 510 374 45
AgeI A'CCGG_T
Cuts at: 0 3920 5526
Size: 3920 1606
AhdI GACnn_n'nnGTC
Cuts at: 0 4650 5526
Size: 4650 876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 5526
Size: 26 5500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 5526
Size: 1198 32 4296
Fragments arranged by size:
4296 1198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 234 407 2180 2694 3754 3937 4051 5526
Size: 234 173 1773 514 1060 183 114 1475
Fragments arranged by size:
1773 1475 1060 514 234 183 173 114
ApaI G_GGCC'C
Cuts at: 0 2939 5526
Size: 2939 2587
ApaLI G'TGCA_C
Cuts at: 0 1523 2186 5526
Size: 1523 663 3340
Fragments arranged by size:
3340 1523 663
ApoI r'AATT_y
Cuts at: 0 582 1784 2910 3378 5526
Size: 582 1202 1126 468 2148
Fragments arranged by size:
2148 1202 1126 582 468
AvaI C'yCGr_G
Cuts at: 0 828 1085 1414 1537 2064 2932 5448 5526
Size: 828 257 329 123 527 868 2516 78
Fragments arranged by size:
2516 868 828 527 329 257 123 78
BamHI G'GATC_C
Cuts at: 0 2209 4189 5526
Size: 2209 1980 1337
Fragments arranged by size:
2209 1980 1337
BanI G'GyrC_C
Cuts at: 0 799 1494 2257 5526
Size: 799 695 763 3269
Fragments arranged by size:
3269 799 763 695
BanII G_rGCy'C
Cuts at: 0 2617 2905 2939 3064 3791 4303 4727 4768
Size: 2617 288 34 125 727 512 424 41
Cuts at: 4768 5272 5296 5526
Size: 504 24 230
Fragments arranged by size:
2617 727 512 504 424 288 230 125
41 34 24
BbeI G_GCGC'C
Cuts at: 0 1498 5526
Size: 1498 4028
BbsI GAAGACnn'nnnn_
Cuts at: 0 305 533 598 926 1390 1415 3459 5032
Size: 305 228 65 328 464 25 2044 1573
Cuts at: 5032 5191 5498 5526
Size: 159 307 28
Fragments arranged by size:
2044 1573 464 328 307 305 228 159
65 28 25
BbvCI CC'TCA_GC
Cuts at: 0 1270 2638 3755 3835 5526
Size: 1270 1368 1117 80 1691
Fragments arranged by size:
1691 1368 1270 1117 80
BciVI GTATCCnnnnn_n'
Cuts at: 0 2228 2568 4375 5526
Size: 2228 340 1807 1151
Fragments arranged by size:
2228 1807 1151 340
BclI T'GATC_A
Cuts at: 0 1733 3855 5526
Size: 1733 2122 1671
Fragments arranged by size:
2122 1733 1671
BglI GCCn_nnn'nGGC
Cuts at: 0 1503 1958 2648 3394 3960 5526
Size: 1503 455 690 746 566 1566
Fragments arranged by size:
1566 1503 746 690 566 455
BglII A'GATC_T
Cuts at: 0 1606 2719 3438 5526
Size: 1606 1113 719 2088
Fragments arranged by size:
2088 1606 1113 719
BlpI GC'TnA_GC
Cuts at: 0 252 2410 3699 3950 5271 5526
Size: 252 2158 1289 251 1321 255
Fragments arranged by size:
2158 1321 1289 255 252 251
Bme1580I G_kGCm'C
Cuts at: 0 1527 2190 2939 5526
Size: 1527 663 749 2587
Fragments arranged by size:
2587 1527 749 663
BmgBI CAC'GTC
Cuts at: 0 5030 5526
Size: 5030 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 1786 4723 5526
Size: 1786 2937 803
Fragments arranged by size:
2937 1786 803
BmtI G_CTAG'C
Cuts at: 0 4319 5526
Size: 4319 1207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 1308 2741 4741 5526
Size: 213 1095 1433 2000 785
Fragments arranged by size:
2000 1433 1095 785 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 744 1485 2383 2475 2825 3049 3847
Size: 658 86 741 898 92 350 224 798
Cuts at: 3847 5200 5526
Size: 1353 326
Fragments arranged by size:
1353 898 798 741 658 350 326 224
92 86
Bpu10I CC'TnA_GC
Cuts at: 0 1270 2638 3273 3755 3835 5526
Size: 1270 1368 635 482 80 1691
Fragments arranged by size:
1691 1368 1270 635 482 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 3963 5526
Size: 594 3369 1563
Fragments arranged by size:
3369 1563 594
BsaI GGTCTCn'nnnn_
Cuts at: 0 111 1171 1300 2200 2533 5526
Size: 111 1060 129 900 333 2993
Fragments arranged by size:
2993 1060 900 333 129 111
BsaHI Gr'CG_yC
Cuts at: 0 1495 4245 5526
Size: 1495 2750 1281
Fragments arranged by size:
2750 1495 1281
BsaWI w'CCGG_w
Cuts at: 0 29 2377 3878 3920 5526
Size: 29 2348 1501 42 1606
Fragments arranged by size:
2348 1606 1501 42 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 2356 2386 3594 3624 5526
Size: 364 30 1962 30 1208 30 1902
Fragments arranged by size:
1962 1902 1208 364 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 433 478 1970 3981 5526
Size: 129 304 45 1492 2011 1545
Fragments arranged by size:
2011 1545 1492 304 129 45
BseYI C'CCAG_C
Cuts at: 0 274 407 483 876 1136 2011 2326 2496
Size: 274 133 76 393 260 875 315 170
Cuts at: 2496 2938 2964 3002 5295 5526
Size: 442 26 38 2293 231
Fragments arranged by size:
2293 875 442 393 315 274 260 231
170 133 76 38 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 2270 4719 5325 5526
Size: 247 2023 2449 606 201
Fragments arranged by size:
2449 2023 606 247 201
BsiHKAI G_wGCw'C
Cuts at: 0 1527 2190 2617 3411 3791 4303 4727 5272
Size: 1527 663 427 794 380 512 424 545
Cuts at: 5272 5455 5526
Size: 183 71
Fragments arranged by size:
1527 794 663 545 512 427 424 380
183 71
BsmI GAATG_Cn'
Cuts at: 0 1943 3459 3748 5526
Size: 1943 1516 289 1778
Fragments arranged by size:
1943 1778 1516 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4252 5526
Size: 4252 1274
Bsp1286I G_dGCh'C
Cuts at: 0 1527 2190 2617 2905 2939 3064 3411 3791
Size: 1527 663 427 288 34 125 347 380
Cuts at: 3791 4303 4727 4768 5272 5296 5455 5526
Size: 512 424 41 504 24 159 71
Fragments arranged by size:
1527 663 512 504 427 424 380 347
288 159 125 71 41 34 24
BspEI T'CCGG_A
Cuts at: 0 29 5526
Size: 29 5497
BspHI T'CATG_A
Cuts at: 0 2699 5526
Size: 2699 2827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2257 5526
Size: 2257 3269
BsrBI CCG'CTC
Cuts at: 0 192 5526
Size: 192 5334
BsrDI GCAATG_nn'
Cuts at: 0 4310 5526
Size: 4310 1216
BsrFI r'CCGG_y
Cuts at: 0 3920 5526
Size: 3920 1606
BssSI C'ACGA_G
Cuts at: 0 2530 2610 2680 3041 5526
Size: 2530 80 70 361 2485
Fragments arranged by size:
2530 2485 361 80 70
BstBI TT'CG_AA
Cuts at: 0 2244 4947 5526
Size: 2244 2703 579
Fragments arranged by size:
2703 2244 579
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3010 3027 3685 5526
Size: 3010 17 658 1841
Fragments arranged by size:
3010 1841 658 17
BstYI r'GATC_y
Cuts at: 0 1606 1966 2209 2719 3438 3860 4189 4342
Size: 1606 360 243 510 719 422 329 153
Cuts at: 4342 5145 5232 5400 5526
Size: 803 87 168 126
Fragments arranged by size:
1606 803 719 510 422 360 329 243
168 153 126 87
BstZ17I GTA'TAC
Cuts at: 0 842 5526
Size: 842 4684
Bsu36I CC'TnA_GG
Cuts at: 0 1322 1659 5285 5526
Size: 1322 337 3626 241
Fragments arranged by size:
3626 1322 337 241
BtgI C'CryG_G
Cuts at: 0 122 1359 5111 5224 5263 5526
Size: 122 1237 3752 113 39 263
Fragments arranged by size:
3752 1237 263 122 113 39
BtsI GCAGTG_nn'
Cuts at: 0 801 924 1167 1638 1749 2403 3522 4119
Size: 801 123 243 471 111 654 1119 597
Cuts at: 4119 4707 5192 5526
Size: 588 485 334
Fragments arranged by size:
1119 801 654 597 588 485 471 334
243 123 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2250 2285 4272 4307 5526
Size: 2250 35 1987 35 1219
Fragments arranged by size:
2250 1987 1219 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1116 5526
Size: 1116 4410
EaeI y'GGCC_r
Cuts at: 0 1950 3288 3961 4107 5526
Size: 1950 1338 673 146 1419
Fragments arranged by size:
1950 1419 1338 673 146
EarI CTCTTCn'nnn_
Cuts at: 0 318 410 584 1464 2437 2765 2982 3102
Size: 318 92 174 880 973 328 217 120
Cuts at: 3102 3629 3775 3999 5526
Size: 527 146 224 1527
Fragments arranged by size:
1527 973 880 527 328 318 224 217
174 146 120 92
EcoICRI GAG'CTC
Cuts at: 0 2615 3789 4301 4725 5270 5526
Size: 2615 1174 512 424 545 256
Fragments arranged by size:
2615 1174 545 512 424 256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 383 434 658 736 744 1427 1485
Size: 49 334 51 224 78 8 683 58
Cuts at: 1485 1598 2383 2475 2797 2825 3049 3164 3388
Size: 113 785 92 322 28 224 115 224
Cuts at: 3388 3713 3799 3847 5185 5200 5290 5526
Size: 325 86 48 1338 15 90 236
Fragments arranged by size:
1338 785 683 334 325 322 236 224
224 224 115 113 92 90 86 78
58 51 49 48 28 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1952 5526
Size: 1952 3574
EcoO109I rG'GnC_Cy
Cuts at: 0 3831 5526
Size: 3831 1695
EcoRI G'AATT_C
Cuts at: 0 3378 5526
Size: 3378 2148
EcoRV GAT'ATC
Cuts at: 0 975 4779 4942 5526
Size: 975 3804 163 584
Fragments arranged by size:
3804 975 584 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1628 2153 5526
Size: 1628 525 3373
Fragments arranged by size:
3373 1628 525
FspI TGC'GCA
Cuts at: 0 130 5526
Size: 130 5396
FspAI rTGC'GCAy
Cuts at: 0 130 5526
Size: 130 5396
HaeII r_GCGC'y
Cuts at: 0 198 1498 2064 2180 5526
Size: 198 1300 566 116 3346
Fragments arranged by size:
3346 1300 566 198 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 343 375 438 470 583 615
Size: 298 32 13 32 63 32 113 32
Cuts at: 615 754 786 851 883 1149 1181 2215 2247
Size: 139 32 65 32 266 32 1034 32
Cuts at: 2247 2356 2388 2711 2743 3517 3549 3682 3714
Size: 109 32 323 32 774 32 133 32
Cuts at: 3714 4153 4185 4500 4532 4636 4668 5019 5051
Size: 439 32 315 32 104 32 351 32
Cuts at: 5051 5181 5213 5526
Size: 130 32 313
Fragments arranged by size:
1034 774 439 351 323 315 313 298
266 139 133 130 113 109 104 65
63 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 706 2082 5526
Size: 706 1376 3444
Fragments arranged by size:
3444 1376 706
HindIII A'AGCT_T
Cuts at: 0 1433 1559 2469 5526
Size: 1433 126 910 3057
Fragments arranged by size:
3057 1433 910 126
KasI G'GCGC_C
Cuts at: 0 1494 5526
Size: 1494 4032
KpnI G_GTAC'C
Cuts at: 0 803 5526
Size: 803 4723
MfeI C'AATT_G
Cuts at: 0 4625 4797 5526
Size: 4625 172 729
Fragments arranged by size:
4625 729 172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1259 2015 4371 5526
Size: 161 1098 756 2356 1155
Fragments arranged by size:
2356 1155 1098 756 161
MslI CAynn'nnrTG
Cuts at: 0 458 715 1364 3361 4103 4411 5262 5526
Size: 458 257 649 1997 742 308 851 264
Fragments arranged by size:
1997 851 742 649 458 308 264 257
MspA1I CmG'CkG
Cuts at: 0 6 1136 1269 2872 3142 3316 3777 5310
Size: 6 1130 133 1603 270 174 461 1533
Cuts at: 5310 5526
Size: 216
Fragments arranged by size:
1603 1533 1130 461 270 216 174 133
6
NarI GG'CG_CC
Cuts at: 0 1495 5526
Size: 1495 4031
NcoI C'CATG_G
Cuts at: 0 1359 5111 5224 5263 5526
Size: 1359 3752 113 39 263
Fragments arranged by size:
3752 1359 263 113 39
NheI G'CTAG_C
Cuts at: 0 4315 5526
Size: 4315 1211
NspI r_CATG'y
Cuts at: 0 743 888 933 1479 1879 5526
Size: 743 145 45 546 400 3647
Fragments arranged by size:
3647 743 546 400 145 45
PasI CC'CwG_GG
Cuts at: 0 774 5526
Size: 774 4752
PciI A'CATG_T
Cuts at: 0 884 929 5526
Size: 884 45 4597
Fragments arranged by size:
4597 884 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 722 5526
Size: 722 4804
PfoI T'CCnGG_A
Cuts at: 0 1462 3863 5526
Size: 1462 2401 1663
Fragments arranged by size:
2401 1663 1462
PsiI TTA'TAA
Cuts at: 0 2467 5526
Size: 2467 3059
PspOMI G'GGCC_C
Cuts at: 0 2935 5526
Size: 2935 2591
PspXI vC'TCGA_Gb
Cuts at: 0 828 2064 5448 5526
Size: 828 1236 3384 78
Fragments arranged by size:
3384 1236 828 78
PstI C_TGCA'G
Cuts at: 0 2565 2871 3776 5526
Size: 2565 306 905 1750
Fragments arranged by size:
2565 1750 905 306
PvuII CAG'CTG
Cuts at: 0 1136 1269 2872 3142 3777 5310 5526
Size: 1136 133 1603 270 635 1533 216
Fragments arranged by size:
1603 1533 1136 635 270 216 133
SacI G_AGCT'C
Cuts at: 0 2617 3791 4303 4727 5272 5526
Size: 2617 1174 512 424 545 254
Fragments arranged by size:
2617 1174 545 512 424 254
SalI G'TCGA_C
Cuts at: 0 704 5526
Size: 704 4822
SapI GCTCTTCn'nnn_
Cuts at: 0 3102 3629 3775 5526
Size: 3102 527 146 1751
Fragments arranged by size:
3102 1751 527 146
SfcI C'TryA_G
Cuts at: 0 2456 2561 2867 3137 3772 5526
Size: 2456 105 306 270 635 1754
Fragments arranged by size:
2456 1754 635 306 270 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1958 5526
Size: 1958 3568
SfoI GGC'GCC
Cuts at: 0 1496 5526
Size: 1496 4030
SmaI CCC'GGG
Cuts at: 0 1087 1539 2934 5526
Size: 1087 452 1395 2592
Fragments arranged by size:
2592 1395 1087 452
SmlI C'TyrA_G
Cuts at: 0 573 828 2064 2472 3978 5448 5526
Size: 573 255 1236 408 1506 1470 78
Fragments arranged by size:
1506 1470 1236 573 408 255 78
StyI C'CwwG_G
Cuts at: 0 158 978 1359 1485 2261 4290 5111 5224
Size: 158 820 381 126 776 2029 821 113
Cuts at: 5224 5263 5526
Size: 39 263
Fragments arranged by size:
2029 821 820 776 381 263 158 126
113 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 766 940 2250 4554 5235 5526
Size: 475 291 174 1310 2304 681 291
Fragments arranged by size:
2304 1310 681 475 291 291 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3594 3626 5526
Size: 3594 32 1900
Fragments arranged by size:
3594 1900 32
Tth111I GACn'n_nGTC
Cuts at: 0 597 1389 4999 5526
Size: 597 792 3610 527
Fragments arranged by size:
3610 792 597 527
XbaI T'CTAG_A
Cuts at: 0 492 4866 5128 5526
Size: 492 4374 262 398
Fragments arranged by size:
4374 492 398 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1366 3508 4638 5526
Size: 1366 2142 1130 888
Fragments arranged by size:
2142 1366 1130 888
XhoI C'TCGA_G
Cuts at: 0 828 2064 5448 5526
Size: 828 1236 3384 78
Fragments arranged by size:
3384 1236 828 78
XmaI C'CCGG_G
Cuts at: 0 1085 1537 2932 5526
Size: 1085 452 1395 2594
Fragments arranged by size:
2594 1395 1085 452
XmnI GAAnn'nnTTC
Cuts at: 0 3086 5526
Size: 3086 2440
ZraI GAC'GTC
Cuts at: 0 4246 5526
Size: 4246 1280
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MslI MspA1I NarI NcoI NheI NspI PasI
PciI PflMI PfoI PsiI PspOMI PspXI PstI PvuII
SacI SalI SapI SfcI SfiI SfoI SmaI SmlI
StyI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AleI AscI AseI AsiSI AvrII BaeI
BcgI BfrBI BsaAI BsaBI BsiEI BsiWI BsrGI BssHII
BstAPI BstEII BtgZI ClaI DraI DrdI EagI EciI
FseI HpaI MluI MscI NaeI NdeI NgoMIV NotI
NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI
PsrI PvuI RsrII SacII SanDI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI