Restriction map for
pF1KSDA0940
(Linear) (Six-base) MAPSORT of: KIAA0940.seq Check: 9875 from: 1 to: 2052
KIAA0940.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 1364 2052
Size: 1364 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1738 1786 2052
Size: 1738 48 266
Fragments arranged by size:
1738 266 48
AfeI AGC'GCT
Cuts at: 0 1785 2052
Size: 1785 267
AflII C'TTAA_G
Cuts at: 0 1227 2052
Size: 1227 825
AlwNI CAG_nnn'CTG
Cuts at: 0 178 329 685 2052
Size: 178 151 356 1367
Fragments arranged by size:
1367 356 178 151
ApoI r'AATT_y
Cuts at: 0 1110 1535 2052
Size: 1110 425 517
Fragments arranged by size:
1110 517 425
AvaI C'yCGr_G
Cuts at: 0 1973 2052
Size: 1973 79
BanI G'GyrC_C
Cuts at: 0 291 340 594 843 2052
Size: 291 49 254 249 1209
Fragments arranged by size:
1209 291 254 249 49
BanII G_rGCy'C
Cuts at: 0 287 2052
Size: 287 1765
BbeI G_GCGC'C
Cuts at: 0 598 2052
Size: 598 1454
BbsI GAAGACnn'nnnn_
Cuts at: 0 429 659 2052
Size: 429 230 1393
Fragments arranged by size:
1393 429 230
BbvCI CC'TCA_GC
Cuts at: 0 166 260 2052
Size: 166 94 1792
Fragments arranged by size:
1792 166 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 882 916 2052
Size: 882 34 1136
Fragments arranged by size:
1136 882 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 105 2052
Size: 105 1947
BclI T'GATC_A
Cuts at: 0 1077 1206 1658 1854 2052
Size: 1077 129 452 196 198
Fragments arranged by size:
1077 452 198 196 129
BglI GCCn_nnn'nGGC
Cuts at: 0 187 622 709 2052
Size: 187 435 87 1343
Fragments arranged by size:
1343 435 187 87
Bme1580I G_kGCm'C
Cuts at: 0 343 1882 2052
Size: 343 1539 170
Fragments arranged by size:
1539 343 170
BmgBI CAC'GTC
Cuts at: 0 2034 2052
Size: 2034 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 379 427 2052
Size: 379 48 1625
Fragments arranged by size:
1625 379 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1458 2052
Size: 1458 594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 873 2052
Size: 774 99 1179
Fragments arranged by size:
1179 774 99
Bpu10I CC'TnA_GC
Cuts at: 0 166 260 2052
Size: 166 94 1792
Fragments arranged by size:
1792 166 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 2052
Size: 261 1791
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 2052
Size: 121 1931
BsaHI Gr'CG_yC
Cuts at: 0 595 2052
Size: 595 1457
BsaWI w'CCGG_w
Cuts at: 0 968 1007 2052
Size: 968 39 1045
Fragments arranged by size:
1045 968 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 248 624 2052
Size: 111 137 376 1428
Fragments arranged by size:
1428 376 137 111
BseYI C'CCAG_C
Cuts at: 0 35 41 47 171 479 586 1955 2052
Size: 35 6 6 124 308 107 1369 97
Fragments arranged by size:
1369 308 124 107 97 35 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1816 2052
Size: 1816 236
BsiEI CG_ry'CG
Cuts at: 0 815 2022 2052
Size: 815 1207 30
Fragments arranged by size:
1207 815 30
BsiWI C'GTAC_G
Cuts at: 0 626 2052
Size: 626 1426
BsmI GAATG_Cn'
Cuts at: 0 750 1747 2052
Size: 750 997 305
Fragments arranged by size:
997 750 305
Bsp1286I G_dGCh'C
Cuts at: 0 287 343 1882 2052
Size: 287 56 1539 170
Fragments arranged by size:
1539 287 170 56
BspEI T'CCGG_A
Cuts at: 0 968 2052
Size: 968 1084
BspHI T'CATG_A
Cuts at: 0 644 1080 2052
Size: 644 436 972
Fragments arranged by size:
972 644 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1334 2052
Size: 1334 718
BsrBI CCG'CTC
Cuts at: 0 228 572 855 873 2052
Size: 228 344 283 18 1179
Fragments arranged by size:
1179 344 283 228 18
BsrDI GCAATG_nn'
Cuts at: 0 1661 1769 2052
Size: 1661 108 283
Fragments arranged by size:
1661 283 108
BsrFI r'CCGG_y
Cuts at: 0 159 522 2052
Size: 159 363 1530
Fragments arranged by size:
1530 363 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 685 2052
Size: 685 1367
Bsu36I CC'TnA_GG
Cuts at: 0 278 2052
Size: 278 1774
BtgI C'CryG_G
Cuts at: 0 287 335 620 1200 1543 2052
Size: 287 48 285 580 343 509
Fragments arranged by size:
580 509 343 287 285 48
BtsI GCAGTG_nn'
Cuts at: 0 161 1328 2052
Size: 161 1167 724
Fragments arranged by size:
1167 724 161
DraIII CAC_nnn'GTG
Cuts at: 0 1888 2052
Size: 1888 164
EaeI y'GGCC_r
Cuts at: 0 630 1737 2052
Size: 630 1107 315
Fragments arranged by size:
1107 630 315
EarI CTCTTCn'nnn_
Cuts at: 0 409 2052
Size: 409 1643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 873 1738 1786 2052
Size: 774 99 865 48 266
Fragments arranged by size:
865 774 266 99 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1091 1406 2052
Size: 1091 315 646
Fragments arranged by size:
1091 646 315
EcoO109I rG'GnC_Cy
Cuts at: 0 798 1016 2052
Size: 798 218 1036
Fragments arranged by size:
1036 798 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1409 2052
Size: 1409 643
HaeII r_GCGC'y
Cuts at: 0 570 598 617 1787 2052
Size: 570 28 19 1170 265
Fragments arranged by size:
1170 570 265 28 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 1148 1180 2052
Size: 234 32 882 32 872
Fragments arranged by size:
882 872 234 32 32
HincII GTy'rAC
Cuts at: 0 390 429 2052
Size: 390 39 1623
Fragments arranged by size:
1623 390 39
HindIII A'AGCT_T
Cuts at: 0 1451 2052
Size: 1451 601
KasI G'GCGC_C
Cuts at: 0 594 2052
Size: 594 1458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1025 1272 2052
Size: 791 234 247 780
Fragments arranged by size:
791 780 247 234
MspA1I CmG'CkG
Cuts at: 0 60 178 289 622 685 722 2001 2045
Size: 60 118 111 333 63 37 1279 44
Cuts at: 2045 2052
Size: 7
Fragments arranged by size:
1279 333 118 111 63 60 44 37
7
NaeI GCC'GGC
Cuts at: 0 161 524 2052
Size: 161 363 1528
Fragments arranged by size:
1528 363 161
NarI GG'CG_CC
Cuts at: 0 595 2052
Size: 595 1457
NcoI C'CATG_G
Cuts at: 0 1200 1543 2052
Size: 1200 343 509
Fragments arranged by size:
1200 509 343
NdeI CA'TA_TG
Cuts at: 0 1842 2052
Size: 1842 210
NgoMIV G'CCGG_C
Cuts at: 0 159 522 2052
Size: 159 363 1530
Fragments arranged by size:
1530 363 159
PasI CC'CwG_GG
Cuts at: 0 773 2052
Size: 773 1279
PflMI CCAn_nnn'nTGG
Cuts at: 0 773 947 2052
Size: 773 174 1105
Fragments arranged by size:
1105 773 174
PfoI T'CCnGG_A
Cuts at: 0 946 1122 1187 2052
Size: 946 176 65 865
Fragments arranged by size:
946 865 176 65
PpuMI rG'GwC_Cy
Cuts at: 0 798 2052
Size: 798 1254
PstI C_TGCA'G
Cuts at: 0 788 1937 2052
Size: 788 1149 115
Fragments arranged by size:
1149 788 115
PvuII CAG'CTG
Cuts at: 0 722 2052
Size: 722 1330
RsrII CG'GwC_CG
Cuts at: 0 971 2052
Size: 971 1081
SacII CC_GC'GG
Cuts at: 0 290 623 2052
Size: 290 333 1429
Fragments arranged by size:
1429 333 290
SanDI GG'GwC_CC
Cuts at: 0 798 2052
Size: 798 1254
SapI GCTCTTCn'nnn_
Cuts at: 0 409 2052
Size: 409 1643
SbfI CC_TGCA'GG
Cuts at: 0 788 2052
Size: 788 1264
SexAI A'CCwGG_T
Cuts at: 0 328 2052
Size: 328 1724
SfcI C'TryA_G
Cuts at: 0 784 1933 2052
Size: 784 1149 119
Fragments arranged by size:
1149 784 119
SfoI GGC'GCC
Cuts at: 0 596 2052
Size: 596 1456
SmlI C'TyrA_G
Cuts at: 0 240 1227 2052
Size: 240 987 825
Fragments arranged by size:
987 825 240
SspI AAT'ATT
Cuts at: 0 1137 2052
Size: 1137 915
StyI C'CwwG_G
Cuts at: 0 1200 1543 2052
Size: 1200 343 509
Fragments arranged by size:
1200 509 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 823 988 2052
Size: 817 6 165 1064
Fragments arranged by size:
1064 817 165 6
TatI w'GTAC_w
Cuts at: 0 1446 2052
Size: 1446 606
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1539 1571 2052
Size: 1539 32 481
Fragments arranged by size:
1539 481 32
XbaI T'CTAG_A
Cuts at: 0 1276 2052
Size: 1276 776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 948 2052
Size: 948 1104
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI
Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV PasI PflMI
PfoI PpuMI PstI PvuII RsrII SacII SanDI SapI
SbfI SexAI SfcI SfoI SmlI SspI StyI TaqII
TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BfrBI BglII BlpI BmtI BsaAI BsaBI
BsaXI BsiHKAI BsmBI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MfeI MluI MscI MslI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI SacI SalI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XhoI XmaI XmnI ZraI