Restriction map for
pF1KSDA0941
(Linear) (Six-base) MAPSORT of: KIAA0941.seq Check: 3023 from: 1 to: 1536
KIAA0941.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 1536
Size: 288 1248
Acc65I G'GTAC_C
Cuts at: 0 94 1536
Size: 94 1442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 742 1536
Size: 94 648 794
Fragments arranged by size:
794 648 94
AlwNI CAG_nnn'CTG
Cuts at: 0 1377 1536
Size: 1377 159
ApoI r'AATT_y
Cuts at: 0 286 595 940 1008 1231 1522 1536
Size: 286 309 345 68 223 291 14
Fragments arranged by size:
345 309 291 286 223 68 14
AvaI C'yCGr_G
Cuts at: 0 1429 1536
Size: 1429 107
BanI G'GyrC_C
Cuts at: 0 94 730 1536
Size: 94 636 806
Fragments arranged by size:
806 636 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 701 900 1298 1536
Size: 542 159 199 398 238
Fragments arranged by size:
542 398 238 199 159
BbvCI CC'TCA_GC
Cuts at: 0 656 1536
Size: 656 880
BfrBI ATG'CAT
Cuts at: 0 1171 1536
Size: 1171 365
BglII A'GATC_T
Cuts at: 0 69 481 1111 1536
Size: 69 412 630 425
Fragments arranged by size:
630 425 412 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 1472 1536
Size: 1472 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 1536
Size: 207 1329
Bpu10I CC'TnA_GC
Cuts at: 0 656 1536
Size: 656 880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1536
Size: 187 1349
BsaAI yAC'GTr
Cuts at: 0 42 1536
Size: 42 1494
BsaBI GATnn'nnATC
Cuts at: 0 366 1536
Size: 366 1170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 468 498 1536
Size: 468 30 1038
Fragments arranged by size:
1038 468 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1536
Size: 199 1337
BseYI C'CCAG_C
Cuts at: 0 123 1536
Size: 123 1413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1536
Size: 64 1472
BsiHKAI G_wGCw'C
Cuts at: 0 59 1536
Size: 59 1477
BsmI GAATG_Cn'
Cuts at: 0 675 1536
Size: 675 861
Bsp1286I G_dGCh'C
Cuts at: 0 59 1536
Size: 59 1477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 288 1536
Size: 36 252 1248
Fragments arranged by size:
1248 252 36
BsrDI GCAATG_nn'
Cuts at: 0 1293 1536
Size: 1293 243
BstYI r'GATC_y
Cuts at: 0 69 481 1111 1135 1536
Size: 69 412 630 24 401
Fragments arranged by size:
630 412 401 69 24
Bsu36I CC'TnA_GG
Cuts at: 0 1297 1399 1536
Size: 1297 102 137
Fragments arranged by size:
1297 137 102
BtsI GCAGTG_nn'
Cuts at: 0 949 1331 1536
Size: 949 382 205
Fragments arranged by size:
949 382 205
EarI CTCTTCn'nnn_
Cuts at: 0 1355 1536
Size: 1355 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 742 1536
Size: 94 648 794
Fragments arranged by size:
794 648 94
EcoO109I rG'GnC_Cy
Cuts at: 0 266 652 698 1536
Size: 266 386 46 838
Fragments arranged by size:
838 386 266 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1414 1536
Size: 1414 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1192 1224 1536
Size: 1144 32 16 32 312
Fragments arranged by size:
1144 312 32 32 16
HincII GTy'rAC
Cuts at: 0 1211 1536
Size: 1211 325
HindIII A'AGCT_T
Cuts at: 0 828 1536
Size: 828 708
KpnI G_GTAC'C
Cuts at: 0 98 1536
Size: 98 1438
MfeI C'AATT_G
Cuts at: 0 1025 1536
Size: 1025 511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1303 1536
Size: 58 1245 233
Fragments arranged by size:
1245 233 58
MspA1I CmG'CkG
Cuts at: 0 123 1536
Size: 123 1413
NdeI CA'TA_TG
Cuts at: 0 704 1271 1497 1536
Size: 704 567 226 39
Fragments arranged by size:
704 567 226 39
NsiI A_TGCA'T
Cuts at: 0 1173 1536
Size: 1173 363
NspI r_CATG'y
Cuts at: 0 579 1536
Size: 579 957
PflMI CCAn_nnn'nTGG
Cuts at: 0 793 1536
Size: 793 743
PfoI T'CCnGG_A
Cuts at: 0 1307 1421 1507 1536
Size: 1307 114 86 29
Fragments arranged by size:
1307 114 86 29
PmlI CAC'GTG
Cuts at: 0 42 1536
Size: 42 1494
PpuMI rG'GwC_Cy
Cuts at: 0 266 652 698 1536
Size: 266 386 46 838
Fragments arranged by size:
838 386 266 46
PshAI GACnn'nnGTC
Cuts at: 0 666 1536
Size: 666 870
PspXI vC'TCGA_Gb
Cuts at: 0 1429 1536
Size: 1429 107
PstI C_TGCA'G
Cuts at: 0 1338 1536
Size: 1338 198
PvuII CAG'CTG
Cuts at: 0 123 1536
Size: 123 1413
SanDI GG'GwC_CC
Cuts at: 0 698 1536
Size: 698 838
ScaI AGT'ACT
Cuts at: 0 139 570 1536
Size: 139 431 966
Fragments arranged by size:
966 431 139
SfcI C'TryA_G
Cuts at: 0 149 1334 1369 1536
Size: 149 1185 35 167
Fragments arranged by size:
1185 167 149 35
SmlI C'TyrA_G
Cuts at: 0 166 1429 1536
Size: 166 1263 107
Fragments arranged by size:
1263 166 107
SspI AAT'ATT
Cuts at: 0 417 1536
Size: 417 1119
StuI AGG'CCT
Cuts at: 0 190 1536
Size: 190 1346
StyI C'CwwG_G
Cuts at: 0 955 1536
Size: 955 581
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1429 1536
Size: 1429 107
TatI w'GTAC_w
Cuts at: 0 137 568 1536
Size: 137 431 968
Fragments arranged by size:
968 431 137
XhoI C'TCGA_G
Cuts at: 0 1429 1536
Size: 1429 107
XmnI GAAnn'nnTTC
Cuts at: 0 537 1364 1536
Size: 537 827 172
Fragments arranged by size:
827 537 172
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApoI AvaI BanI BbsI
BbvCI BfrBI BglII BmrI BplI Bpu10I BpuEI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstYI Bsu36I BtsI EarI Eco57MI EcoO109I
FalI Hin4I HincII HindIII KpnI MfeI MmeI MspA1I
NdeI NsiI NspI PflMI PfoI PmlI PpuMI PshAI
PspXI PstI PvuII SanDI ScaI SfcI SmlI SspI
StuI StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmtI BpmI BsaI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI MluI MscI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XcmI XmaI ZraI