Restriction map for
pF1KA0942
(Linear) (Six-base) MAPSORT of: KIAA0942.seq Check: 4169 from: 1 to: 1539
KIAA0942.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 1286 1539
Size: 1286 253
Acc65I G'GTAC_C
Cuts at: 0 127 1539
Size: 127 1412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 871 929 1213 1234 1432 1539
Size: 283 588 58 284 21 198 107
Fragments arranged by size:
588 284 283 198 107 58 21
AflIII A'CryG_T
Cuts at: 0 986 1481 1539
Size: 986 495 58
Fragments arranged by size:
986 495 58
AhdI GACnn_n'nnGTC
Cuts at: 0 689 1539
Size: 689 850
ApaLI G'TGCA_C
Cuts at: 0 937 1539
Size: 937 602
ApoI r'AATT_y
Cuts at: 0 89 232 779 1539
Size: 89 143 547 760
Fragments arranged by size:
760 547 143 89
AvaI C'yCGr_G
Cuts at: 0 1425 1539
Size: 1425 114
BanI G'GyrC_C
Cuts at: 0 127 1539
Size: 127 1412
BanII G_rGCy'C
Cuts at: 0 7 1042 1539
Size: 7 1035 497
Fragments arranged by size:
1035 497 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 811 1539
Size: 811 728
BciVI GTATCCnnnnn_n'
Cuts at: 0 1440 1539
Size: 1440 99
Bme1580I G_kGCm'C
Cuts at: 0 941 1539
Size: 941 598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1263 1539
Size: 1263 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 1336 1539
Size: 1032 304 203
Fragments arranged by size:
1032 304 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1342 1539
Size: 329 1013 197
Fragments arranged by size:
1013 329 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 1539
Size: 97 1442
BsaHI Gr'CG_yC
Cuts at: 0 1283 1539
Size: 1283 256
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 1198 1539
Size: 670 528 341
Fragments arranged by size:
670 528 341
BseYI C'CCAG_C
Cuts at: 0 37 1539
Size: 37 1502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1539
Size: 1082 457
BsiHKAI G_wGCw'C
Cuts at: 0 85 941 1244 1539
Size: 85 856 303 295
Fragments arranged by size:
856 303 295 85
BsmI GAATG_Cn'
Cuts at: 0 322 1352 1539
Size: 322 1030 187
Fragments arranged by size:
1030 322 187
Bsp1286I G_dGCh'C
Cuts at: 0 7 85 941 1042 1244 1539
Size: 7 78 856 101 202 295
Fragments arranged by size:
856 295 202 101 78 7
BspHI T'CATG_A
Cuts at: 0 732 1539
Size: 732 807
BsrBI CCG'CTC
Cuts at: 0 1247 1539
Size: 1247 292
BsrDI GCAATG_nn'
Cuts at: 0 39 144 407 429 512 1539
Size: 39 105 263 22 83 1027
Fragments arranged by size:
1027 263 105 83 39 22
BsrGI T'GTAC_A
Cuts at: 0 575 752 1539
Size: 575 177 787
Fragments arranged by size:
787 575 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 484 1233 1539
Size: 159 325 749 306
Fragments arranged by size:
749 325 306 159
BstYI r'GATC_y
Cuts at: 0 558 1539
Size: 558 981
BtgI C'CryG_G
Cuts at: 0 962 1539
Size: 962 577
BtsI GCAGTG_nn'
Cuts at: 0 1460 1539
Size: 1460 79
DraI TTT'AAA
Cuts at: 0 318 1539
Size: 318 1221
DrdI GACnn_nn'nnGTC
Cuts at: 0 1015 1267 1539
Size: 1015 252 272
Fragments arranged by size:
1015 272 252
EaeI y'GGCC_r
Cuts at: 0 161 474 1235 1539
Size: 161 313 761 304
Fragments arranged by size:
761 313 304 161
EarI CTCTTCn'nnn_
Cuts at: 0 12 617 905 1539
Size: 12 605 288 634
Fragments arranged by size:
634 605 288 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 871 929 1032 1213 1234 1336 1432
Size: 283 588 58 103 181 21 102 96
Cuts at: 1432 1539
Size: 107
Fragments arranged by size:
588 283 181 107 103 102 96 58
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 486 518 675 707 1482 1514
Size: 279 32 175 32 157 32 775 32
Cuts at: 1514 1539
Size: 25
Fragments arranged by size:
775 279 175 157 32 32 32 32
25
HindIII A'AGCT_T
Cuts at: 0 598 1539
Size: 598 941
KpnI G_GTAC'C
Cuts at: 0 131 1539
Size: 131 1408
MfeI C'AATT_G
Cuts at: 0 1074 1539
Size: 1074 465
MscI TGG'CCA
Cuts at: 0 163 476 1539
Size: 163 313 1063
Fragments arranged by size:
1063 313 163
MspA1I CmG'CkG
Cuts at: 0 180 1087 1539
Size: 180 907 452
Fragments arranged by size:
907 452 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 948 1314 1539
Size: 948 366 225
Fragments arranged by size:
948 366 225
PspXI vC'TCGA_Gb
Cuts at: 0 1425 1539
Size: 1425 114
PstI C_TGCA'G
Cuts at: 0 1407 1539
Size: 1407 132
PvuII CAG'CTG
Cuts at: 0 180 1087 1539
Size: 180 907 452
Fragments arranged by size:
907 452 180
SapI GCTCTTCn'nnn_
Cuts at: 0 12 1539
Size: 12 1527
ScaI AGT'ACT
Cuts at: 0 651 705 1539
Size: 651 54 834
Fragments arranged by size:
834 651 54
SfcI C'TryA_G
Cuts at: 0 1403 1539
Size: 1403 136
SmlI C'TyrA_G
Cuts at: 0 344 1357 1425 1539
Size: 344 1013 68 114
Fragments arranged by size:
1013 344 114 68
SpeI A'CTAG_T
Cuts at: 0 243 1539
Size: 243 1296
SspI AAT'ATT
Cuts at: 0 483 1539
Size: 483 1056
StuI AGG'CCT
Cuts at: 0 901 1539
Size: 901 638
StyI C'CwwG_G
Cuts at: 0 216 554 956 1277 1539
Size: 216 338 402 321 262
Fragments arranged by size:
402 338 321 262 216
TatI w'GTAC_w
Cuts at: 0 575 649 703 752 846 1292 1539
Size: 575 74 54 49 94 446 247
Fragments arranged by size:
575 446 247 94 74 54 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 1539
Size: 138 1401
XhoI C'TCGA_G
Cuts at: 0 1425 1539
Size: 1425 114
ZraI GAC'GTC
Cuts at: 0 1284 1539
Size: 1284 255
Enzymes that do cut:
AatII Acc65I AcuI AflIII AhdI ApaLI ApoI AvaI
BanI BanII BbsI BciVI Bme1580I BplI BpmI BpuEI
BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BsrGI BstXI BstYI BtgI BtsI
DraI DrdI EaeI EarI Eco57MI Hin4I HindIII KpnI
MfeI MscI MspA1I PflMI PspXI PstI PvuII SapI
ScaI SfcI SmlI SpeI SspI StuI StyI TatI
XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI TaqII TstI Tth111I XbaI XmaI
XmnI