Restriction map for
pF1KA0946
(Linear) (Six-base) MAPSORT of: KIAA0946.seq Check: 4705 from: 1 to: 1896
KIAA0946.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1849 1896
Size: 1849 47
Acc65I G'GTAC_C
Cuts at: 0 1883 1896
Size: 1883 13
AfeI AGC'GCT
Cuts at: 0 782 1896
Size: 782 1114
AflIII A'CryG_T
Cuts at: 0 1576 1896
Size: 1576 320
AgeI A'CCGG_T
Cuts at: 0 1338 1896
Size: 1338 558
AhdI GACnn_n'nnGTC
Cuts at: 0 864 1292 1896
Size: 864 428 604
Fragments arranged by size:
864 604 428
AlwNI CAG_nnn'CTG
Cuts at: 0 1322 1879 1896
Size: 1322 557 17
Fragments arranged by size:
1322 557 17
ApoI r'AATT_y
Cuts at: 0 369 898 1099 1384 1795 1896
Size: 369 529 201 285 411 101
Fragments arranged by size:
529 411 369 285 201 101
AseI AT'TA_AT
Cuts at: 0 454 1896
Size: 454 1442
BamHI G'GATC_C
Cuts at: 0 612 1896
Size: 612 1284
BanI G'GyrC_C
Cuts at: 0 1883 1896
Size: 1883 13
BclI T'GATC_A
Cuts at: 0 818 1389 1896
Size: 818 571 507
Fragments arranged by size:
818 571 507
BfrBI ATG'CAT
Cuts at: 0 1062 1896
Size: 1062 834
Bme1580I G_kGCm'C
Cuts at: 0 77 1896
Size: 77 1819
BmgBI CAC'GTC
Cuts at: 0 1118 1896
Size: 1118 778
BmrI ACTGGGnnnn_n'
Cuts at: 0 396 1518 1896
Size: 396 1122 378
Fragments arranged by size:
1122 396 378
BmtI G_CTAG'C
Cuts at: 0 703 1896
Size: 703 1193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1896
Size: 761 1135
BsaI GGTCTCn'nnnn_
Cuts at: 0 1519 1896
Size: 1519 377
BsaAI yAC'GTr
Cuts at: 0 1577 1896
Size: 1577 319
BsaHI Gr'CG_yC
Cuts at: 0 574 1896
Size: 574 1322
BsaWI w'CCGG_w
Cuts at: 0 255 1338 1896
Size: 255 1083 558
Fragments arranged by size:
1083 558 255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 734 764 1896
Size: 734 30 1132
Fragments arranged by size:
1132 734 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 268 1896
Size: 25 243 1628
Fragments arranged by size:
1628 243 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1445 1896
Size: 461 984 451
Fragments arranged by size:
984 461 451
BsmI GAATG_Cn'
Cuts at: 0 1707 1896
Size: 1707 189
Bsp1286I G_dGCh'C
Cuts at: 0 77 1896
Size: 77 1819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 226 1896
Size: 226 1670
BsrDI GCAATG_nn'
Cuts at: 0 379 1069 1395 1896
Size: 379 690 326 501
Fragments arranged by size:
690 501 379 326
BsrFI r'CCGG_y
Cuts at: 0 1338 1896
Size: 1338 558
BstBI TT'CG_AA
Cuts at: 0 331 1896
Size: 331 1565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 127 386 1896
Size: 127 259 1510
Fragments arranged by size:
1510 259 127
BstYI r'GATC_y
Cuts at: 0 444 612 1224 1876 1896
Size: 444 168 612 652 20
Fragments arranged by size:
652 612 444 168 20
BstZ17I GTA'TAC
Cuts at: 0 1850 1896
Size: 1850 46
BtgI C'CryG_G
Cuts at: 0 120 413 1402 1761 1896
Size: 120 293 989 359 135
Fragments arranged by size:
989 359 293 135 120
BtsI GCAGTG_nn'
Cuts at: 0 677 701 1896
Size: 677 24 1195
Fragments arranged by size:
1195 677 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1338 1373 1896
Size: 1338 35 523
Fragments arranged by size:
1338 523 35
DraI TTT'AAA
Cuts at: 0 1725 1896
Size: 1725 171
DraIII CAC_nnn'GTG
Cuts at: 0 607 1896
Size: 607 1289
EarI CTCTTCn'nnn_
Cuts at: 0 1244 1896
Size: 1244 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1896
Size: 49 1847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1896
Size: 761 1135
EcoO109I rG'GnC_Cy
Cuts at: 0 363 1896
Size: 363 1533
EcoRI G'AATT_C
Cuts at: 0 369 1896
Size: 369 1527
EcoRV GAT'ATC
Cuts at: 0 1667 1896
Size: 1667 229
HaeII r_GCGC'y
Cuts at: 0 784 1896
Size: 784 1112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 1278 1310 1855 1887 1896
Size: 850 32 396 32 545 32 9
Fragments arranged by size:
850 545 396 32 32 32 9
HincII GTy'rAC
Cuts at: 0 651 1896
Size: 651 1245
HpaI GTT'AAC
Cuts at: 0 651 1896
Size: 651 1245
KpnI G_GTAC'C
Cuts at: 0 1887 1896
Size: 1887 9
MfeI C'AATT_G
Cuts at: 0 512 1896
Size: 512 1384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1896
Size: 640 1256
MslI CAynn'nnrTG
Cuts at: 0 28 125 158 418 1358 1630 1766 1896
Size: 28 97 33 260 940 272 136 130
Fragments arranged by size:
940 272 260 136 130 97 33 28
MspA1I CmG'CkG
Cuts at: 0 6 1373 1890 1896
Size: 6 1367 517 6
Fragments arranged by size:
1367 517 6 6
NcoI C'CATG_G
Cuts at: 0 120 413 1402 1761 1896
Size: 120 293 989 359 135
Fragments arranged by size:
989 359 293 135 120
NheI G'CTAG_C
Cuts at: 0 699 1896
Size: 699 1197
NsiI A_TGCA'T
Cuts at: 0 1064 1896
Size: 1064 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 1103 1896
Size: 1103 793
PmlI CAC'GTG
Cuts at: 0 1577 1896
Size: 1577 319
PpuMI rG'GwC_Cy
Cuts at: 0 363 1896
Size: 363 1533
PsiI TTA'TAA
Cuts at: 0 116 1896
Size: 116 1780
PstI C_TGCA'G
Cuts at: 0 1318 1896
Size: 1318 578
PvuII CAG'CTG
Cuts at: 0 1890 1896
Size: 1890 6
SapI GCTCTTCn'nnn_
Cuts at: 0 1244 1896
Size: 1244 652
SfcI C'TryA_G
Cuts at: 0 1019 1314 1896
Size: 1019 295 582
Fragments arranged by size:
1019 582 295
SspI AAT'ATT
Cuts at: 0 972 1779 1896
Size: 972 807 117
Fragments arranged by size:
972 807 117
StyI C'CwwG_G
Cuts at: 0 120 413 654 1077 1402 1538 1585 1761
Size: 120 293 241 423 325 136 47 176
Cuts at: 1761 1896
Size: 135
Fragments arranged by size:
423 325 293 241 176 136 135 120
47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 1395 1620 1896
Size: 101 1294 225 276
Fragments arranged by size:
1294 276 225 101
TatI w'GTAC_w
Cuts at: 0 1023 1896
Size: 1023 873
Tth111I GACn'n_nGTC
Cuts at: 0 181 1896
Size: 181 1715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 118 1896
Size: 118 1778
Enzymes that do cut:
AccI Acc65I AfeI AflIII AgeI AhdI AlwNI ApoI
AseI BamHI BanI BclI BfrBI Bme1580I BmgBI BmrI
BmtI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BstBI BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EarI
EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HpaI KpnI MfeI MmeI MslI MspA1I NcoI NheI
NsiI PflMI PmlI PpuMI PsiI PstI PvuII SapI
SfcI SspI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AleI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
BplI Bpu10I BpuEI BsaBI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstEII Bsu36I BtgZI ClaI DrdI EaeI EagI EcoICRI
EcoNI FalI FseI FspI FspAI HindIII KasI MluI
MscI NaeI NarI NdeI NgoMIV NotI NruI NspI
PacI PasI PciI PfoI PmeI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XhoI XmaI XmnI ZraI