Restriction map for
pF1KSDA0948
(Linear) (Six-base) MAPSORT of: KIAA0948.seq Check: 3575 from: 1 to: 1563
KIAA0948.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 201 885 1563
Size: 201 684 678
Fragments arranged by size:
684 678 201
AatII G_ACGT'C
Cuts at: 0 1466 1563
Size: 1466 97
Acc65I G'GTAC_C
Cuts at: 0 430 1563
Size: 430 1133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1189 1563
Size: 822 367 374
Fragments arranged by size:
822 374 367
AleI CACnn'nnGTG
Cuts at: 0 670 1563
Size: 670 893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1417 1563
Size: 1417 146
AlwNI CAG_nnn'CTG
Cuts at: 0 606 1029 1563
Size: 606 423 534
Fragments arranged by size:
606 534 423
ApaI G_GGCC'C
Cuts at: 0 915 1115 1563
Size: 915 200 448
Fragments arranged by size:
915 448 200
ApoI r'AATT_y
Cuts at: 0 970 1563
Size: 970 593
AvaI C'yCGr_G
Cuts at: 0 1066 1074 1204 1563
Size: 1066 8 130 359
Fragments arranged by size:
1066 359 130 8
BanI G'GyrC_C
Cuts at: 0 430 1293 1510 1563
Size: 430 863 217 53
Fragments arranged by size:
863 430 217 53
BanII G_rGCy'C
Cuts at: 0 254 915 1115 1563
Size: 254 661 200 448
Fragments arranged by size:
661 448 254 200
BbeI G_GCGC'C
Cuts at: 0 1514 1563
Size: 1514 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 1563
Size: 734 829
BbvCI CC'TCA_GC
Cuts at: 0 123 1116 1241 1547 1563
Size: 123 993 125 306 16
Fragments arranged by size:
993 306 125 123 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 11 45 1563
Size: 11 34 1518
Fragments arranged by size:
1518 34 11
BglI GCCn_nnn'nGGC
Cuts at: 0 559 1563
Size: 559 1004
BlpI GC'TnA_GC
Cuts at: 0 614 1563
Size: 614 949
Bme1580I G_kGCm'C
Cuts at: 0 915 1115 1563
Size: 915 200 448
Fragments arranged by size:
915 448 200
BmgBI CAC'GTC
Cuts at: 0 738 1563
Size: 738 825
BmrI ACTGGGnnnn_n'
Cuts at: 0 1325 1563
Size: 1325 238
BmtI G_CTAG'C
Cuts at: 0 1354 1563
Size: 1354 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 612 1563
Size: 612 951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 277 901 1078 1319 1563
Size: 56 221 624 177 241 244
Fragments arranged by size:
624 244 241 221 177 56
Bpu10I CC'TnA_GC
Cuts at: 0 123 1116 1241 1547 1563
Size: 123 993 125 306 16
Fragments arranged by size:
993 306 125 123 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1474 1504 1563
Size: 520 954 30 59
Fragments arranged by size:
954 520 59 30
BsaAI yAC'GTr
Cuts at: 0 29 1563
Size: 29 1534
BsaHI Gr'CG_yC
Cuts at: 0 1463 1511 1563
Size: 1463 48 52
Fragments arranged by size:
1463 52 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 1563
Size: 481 30 1052
Fragments arranged by size:
1052 481 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 1563
Size: 262 1301
BseYI C'CCAG_C
Cuts at: 0 447 858 1179 1226 1307 1404 1563
Size: 447 411 321 47 81 97 159
Fragments arranged by size:
447 411 321 159 97 81 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1563
Size: 798 765
BsiHKAI G_wGCw'C
Cuts at: 0 254 1563
Size: 254 1309
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1156 1563
Size: 1156 407
Bsp1286I G_dGCh'C
Cuts at: 0 254 915 1115 1563
Size: 254 661 200 448
Fragments arranged by size:
661 448 254 200
BspHI T'CATG_A
Cuts at: 0 680 1563
Size: 680 883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 885 1563
Size: 201 684 678
Fragments arranged by size:
684 678 201
BsrDI GCAATG_nn'
Cuts at: 0 308 1563
Size: 308 1255
BsrFI r'CCGG_y
Cuts at: 0 168 1563
Size: 168 1395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 1563
Size: 606 957
BstEII G'GTnAC_C
Cuts at: 0 1134 1563
Size: 1134 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1315 1563
Size: 1315 248
BtgI C'CryG_G
Cuts at: 0 65 1563
Size: 65 1498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 1563
Size: 36 1527
BtsI GCAGTG_nn'
Cuts at: 0 1046 1563
Size: 1046 517
DraIII CAC_nnn'GTG
Cuts at: 0 1437 1563
Size: 1437 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 736 1563
Size: 736 827
EaeI y'GGCC_r
Cuts at: 0 78 1211 1556 1563
Size: 78 1133 345 7
Fragments arranged by size:
1133 345 78 7
EcoICRI GAG'CTC
Cuts at: 0 252 1563
Size: 252 1311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 277 822 901 1078 1189 1319 1563
Size: 56 221 545 79 177 111 130 244
Fragments arranged by size:
545 244 221 177 130 111 79 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 583 1147 1563
Size: 583 564 416
Fragments arranged by size:
583 564 416
EcoO109I rG'GnC_Cy
Cuts at: 0 57 912 1078 1112 1563
Size: 57 855 166 34 451
Fragments arranged by size:
855 451 166 57 34
FspI TGC'GCA
Cuts at: 0 1534 1563
Size: 1534 29
HaeII r_GCGC'y
Cuts at: 0 1190 1514 1563
Size: 1190 324 49
Fragments arranged by size:
1190 324 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 1563
Size: 481 32 1050
Fragments arranged by size:
1050 481 32
KasI G'GCGC_C
Cuts at: 0 1510 1563
Size: 1510 53
KpnI G_GTAC'C
Cuts at: 0 434 1563
Size: 434 1129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1563
Size: 622 941
MscI TGG'CCA
Cuts at: 0 1213 1558 1563
Size: 1213 345 5
Fragments arranged by size:
1213 345 5
MslI CAynn'nnrTG
Cuts at: 0 670 1563
Size: 670 893
MspA1I CmG'CkG
Cuts at: 0 518 1563
Size: 518 1045
NarI GG'CG_CC
Cuts at: 0 1511 1563
Size: 1511 52
NcoI C'CATG_G
Cuts at: 0 65 1563
Size: 65 1498
NheI G'CTAG_C
Cuts at: 0 1350 1563
Size: 1350 213
NspI r_CATG'y
Cuts at: 0 528 1563
Size: 528 1035
PasI CC'CwG_GG
Cuts at: 0 1108 1563
Size: 1108 455
PfoI T'CCnGG_A
Cuts at: 0 254 1563
Size: 254 1309
PmlI CAC'GTG
Cuts at: 0 29 1563
Size: 29 1534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1563
Size: 817 32 714
Fragments arranged by size:
817 714 32
PpuMI rG'GwC_Cy
Cuts at: 0 1078 1563
Size: 1078 485
PspOMI G'GGCC_C
Cuts at: 0 911 1111 1563
Size: 911 200 452
Fragments arranged by size:
911 452 200
PspXI vC'TCGA_Gb
Cuts at: 0 1204 1563
Size: 1204 359
PstI C_TGCA'G
Cuts at: 0 497 896 929 1160 1446 1563
Size: 497 399 33 231 286 117
Fragments arranged by size:
497 399 286 231 117 33
SacI G_AGCT'C
Cuts at: 0 254 1563
Size: 254 1309
SanDI GG'GwC_CC
Cuts at: 0 1078 1563
Size: 1078 485
SbfI CC_TGCA'GG
Cuts at: 0 896 1563
Size: 896 667
ScaI AGT'ACT
Cuts at: 0 1072 1563
Size: 1072 491
SexAI A'CCwGG_T
Cuts at: 0 11 902 1563
Size: 11 891 661
Fragments arranged by size:
891 661 11
SfcI C'TryA_G
Cuts at: 0 493 892 925 1156 1442 1563
Size: 493 399 33 231 286 121
Fragments arranged by size:
493 399 286 231 121 33
SfoI GGC'GCC
Cuts at: 0 1512 1563
Size: 1512 51
SmlI C'TyrA_G
Cuts at: 0 535 1204 1489 1519 1563
Size: 535 669 285 30 44
Fragments arranged by size:
669 535 285 44 30
SphI G_CATG'C
Cuts at: 0 528 1563
Size: 528 1035
StuI AGG'CCT
Cuts at: 0 481 878 1563
Size: 481 397 685
Fragments arranged by size:
685 481 397
StyI C'CwwG_G
Cuts at: 0 65 476 485 1563
Size: 65 411 9 1078
Fragments arranged by size:
1078 411 65 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 408 1218 1563
Size: 150 258 810 345
Fragments arranged by size:
810 345 258 150
TatI w'GTAC_w
Cuts at: 0 539 1070 1563
Size: 539 531 493
Fragments arranged by size:
539 531 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 892 924 1563
Size: 892 32 639
Fragments arranged by size:
892 639 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1475 1563
Size: 1053 422 88
Fragments arranged by size:
1053 422 88
XhoI C'TCGA_G
Cuts at: 0 1204 1563
Size: 1204 359
XmnI GAAnn'nnTTC
Cuts at: 0 1018 1563
Size: 1018 545
ZraI GAC'GTC
Cuts at: 0 1464 1563
Size: 1464 99
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BtgI BtgZI BtsI DraIII DrdI EaeI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NheI
NspI PasI PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI SacI SanDI SbfI ScaI SexAI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BsaI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BsrBI BsrGI BssHII BssSI BstBI BstYI
BstZ17I Bsu36I ClaI CspCI DraI EagI EarI EciI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI
PacI PciI PflMI PmeI PshAI PsiI PsrI PvuI
PvuII RsrII SacII SalI SapI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XmaI