Restriction map for
pF1KA0949
(Linear) (Six-base) MAPSORT of: KIAA0949.seq Check: 9841 from: 1 to: 4635
KIAA0949.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2794 3166 3235 3575 3885 3915 4635
Size: 2794 372 69 340 310 30 720
Fragments arranged by size:
2794 720 372 340 310 69 30
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 410 479 671 977 1376 1666 1957
Size: 100 310 69 192 306 399 290 291
Cuts at: 1957 2182 2359 2377 3151 3339 4613 4635
Size: 225 177 18 774 188 1274 22
Fragments arranged by size:
1274 774 399 310 306 291 290 225
192 188 177 100 69 22 18
AfeI AGC'GCT
Cuts at: 0 3214 4635
Size: 3214 1421
AflII C'TTAA_G
Cuts at: 0 2614 4635
Size: 2614 2021
AhdI GACnn_n'nnGTC
Cuts at: 0 416 4635
Size: 416 4219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 2902 4635
Size: 137 2765 1733
Fragments arranged by size:
2765 1733 137
AlwNI CAG_nnn'CTG
Cuts at: 0 1209 1354 1935 2025 3277 3552 4635
Size: 1209 145 581 90 1252 275 1083
Fragments arranged by size:
1252 1209 1083 581 275 145 90
ApaI G_GGCC'C
Cuts at: 0 4183 4297 4635
Size: 4183 114 338
Fragments arranged by size:
4183 338 114
ApaLI G'TGCA_C
Cuts at: 0 736 1656 2704 4635
Size: 736 920 1048 1931
Fragments arranged by size:
1931 1048 920 736
ApoI r'AATT_y
Cuts at: 0 394 512 875 1048 1405 2599 3031 3458
Size: 394 118 363 173 357 1194 432 427
Cuts at: 3458 3805 3844 3973 4635
Size: 347 39 129 662
Fragments arranged by size:
1194 662 432 427 394 363 357 347
173 129 118 39
AvaI C'yCGr_G
Cuts at: 0 2423 3166 3838 4142 4534 4635
Size: 2423 743 672 304 392 101
Fragments arranged by size:
2423 743 672 392 304 101
BanI G'GyrC_C
Cuts at: 0 1537 2910 3073 3379 3873 4635
Size: 1537 1373 163 306 494 762
Fragments arranged by size:
1537 1373 762 494 306 163
BanII G_rGCy'C
Cuts at: 0 524 689 1217 2045 2414 2876 3395 3656
Size: 524 165 528 828 369 462 519 261
Cuts at: 3656 3755 4183 4297 4635
Size: 99 428 114 338
Fragments arranged by size:
828 528 524 519 462 428 369 338
261 165 114 99
BbeI G_GCGC'C
Cuts at: 0 1541 4635
Size: 1541 3094
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 1330 1654 2170 2404 2576 3444 4008
Size: 345 985 324 516 234 172 868 564
Cuts at: 4008 4556 4619 4635
Size: 548 63 16
Fragments arranged by size:
985 868 564 548 516 345 324 234
172 63 16
BbvCI CC'TCA_GC
Cuts at: 0 680 3712 4125 4635
Size: 680 3032 413 510
Fragments arranged by size:
3032 680 510 413
BciVI GTATCCnnnnn_n'
Cuts at: 0 2691 3775 3796 4635
Size: 2691 1084 21 839
Fragments arranged by size:
2691 1084 839 21
BclI T'GATC_A
Cuts at: 0 899 4635
Size: 899 3736
BglI GCCn_nnn'nGGC
Cuts at: 0 1282 4635
Size: 1282 3353
BlpI GC'TnA_GC
Cuts at: 0 497 4510 4635
Size: 497 4013 125
Fragments arranged by size:
4013 497 125
Bme1580I G_kGCm'C
Cuts at: 0 740 1660 2708 2915 3382 3516 4183 4297
Size: 740 920 1048 207 467 134 667 114
Cuts at: 4297 4635
Size: 338
Fragments arranged by size:
1048 920 740 667 467 338 207 134
114
BmgBI CAC'GTC
Cuts at: 0 3531 4635
Size: 3531 1104
BmrI ACTGGGnnnn_n'
Cuts at: 0 1761 2531 3552 4635
Size: 1761 770 1021 1083
Fragments arranged by size:
1761 1083 1021 770
BmtI G_CTAG'C
Cuts at: 0 3190 4635
Size: 3190 1445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 392 1968 4635
Size: 392 1576 2667
Fragments arranged by size:
2667 1576 392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 502 556 640 847 1078 1120 1132
Size: 130 372 54 84 207 231 42 12
Cuts at: 1132 1183 1231 1321 1468 1687 1758 1993 2047
Size: 51 48 90 147 219 71 235 54
Cuts at: 2047 2371 2576 2845 2986 3499 4353 4501 4635
Size: 324 205 269 141 513 854 148 134
Fragments arranged by size:
854 513 372 324 269 235 231 219
207 205 148 147 141 134 130 90
84 71 54 54 51 48 42 12
Bpu10I CC'TnA_GC
Cuts at: 0 440 680 3712 4125 4635
Size: 440 240 3032 413 510
Fragments arranged by size:
3032 510 440 413 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1201 2108 4009 4635
Size: 263 938 907 1901 626
Fragments arranged by size:
1901 938 907 626 263
BsaI GGTCTCn'nnnn_
Cuts at: 0 1333 2863 3736 4635
Size: 1333 1530 873 899
Fragments arranged by size:
1530 1333 899 873
BsaAI yAC'GTr
Cuts at: 0 1655 2771 4337 4635
Size: 1655 1116 1566 298
Fragments arranged by size:
1655 1566 1116 298
BsaHI Gr'CG_yC
Cuts at: 0 1538 2610 2715 4213 4635
Size: 1538 1072 105 1498 422
Fragments arranged by size:
1538 1498 1072 422 105
BsaWI w'CCGG_w
Cuts at: 0 2414 3728 4635
Size: 2414 1314 907
Fragments arranged by size:
2414 1314 907
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1246 1276 3971 4001 4635
Size: 1246 30 2695 30 634
Fragments arranged by size:
2695 1246 634 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 865 868 1117 1213 1375 1465 1755
Size: 697 168 3 249 96 162 90 290
Cuts at: 1755 3964 4113 4635
Size: 2209 149 522
Fragments arranged by size:
2209 697 522 290 249 168 162 149
96 90 3
BseYI C'CCAG_C
Cuts at: 0 722 1069 2875 2942 3193 3215 3575 3676
Size: 722 347 1806 67 251 22 360 101
Cuts at: 3676 4374 4393 4635
Size: 698 19 242
Fragments arranged by size:
1806 722 698 360 347 251 242 101
67 22 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 2393 3327 3686 3943 4635
Size: 1271 1122 934 359 257 692
Fragments arranged by size:
1271 1122 934 692 359 257
BsiEI CG_ry'CG
Cuts at: 0 2521 4503 4635
Size: 2521 1982 132
Fragments arranged by size:
2521 1982 132
BsiHKAI G_wGCw'C
Cuts at: 0 524 689 740 1196 1217 1660 1889 2045
Size: 524 165 51 456 21 443 229 156
Cuts at: 2045 2414 2531 2708 2767 4349 4635
Size: 369 117 177 59 1582 286
Fragments arranged by size:
1582 524 456 443 369 286 229 177
165 156 117 59 51 21
BsmI GAATG_Cn'
Cuts at: 0 145 1805 2007 4635
Size: 145 1660 202 2628
Fragments arranged by size:
2628 1660 202 145
Bsp1286I G_dGCh'C
Cuts at: 0 524 689 740 1196 1217 1660 1889 2045
Size: 524 165 51 456 21 443 229 156
Cuts at: 2045 2414 2531 2708 2767 2876 2915 3382 3395
Size: 369 117 177 59 109 39 467 13
Cuts at: 3395 3516 3656 3755 4183 4297 4349 4635
Size: 121 140 99 428 114 52 286
Fragments arranged by size:
524 467 456 443 428 369 286 229
177 165 156 140 121 117 114 109
99 59 52 51 39 21 13
BspEI T'CCGG_A
Cuts at: 0 3728 4635
Size: 3728 907
BspHI T'CATG_A
Cuts at: 0 2220 3491 4635
Size: 2220 1271 1144
Fragments arranged by size:
2220 1271 1144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2102 2307 2794 3166 3235 3575 3885 3915
Size: 2102 205 487 372 69 340 310 30
Cuts at: 3915 4635
Size: 720
Fragments arranged by size:
2102 720 487 372 340 310 205 69
30
BsrBI CCG'CTC
Cuts at: 0 1722 3510 4527 4635
Size: 1722 1788 1017 108
Fragments arranged by size:
1788 1722 1017 108
BsrFI r'CCGG_y
Cuts at: 0 3021 4565 4635
Size: 3021 1544 70
Fragments arranged by size:
3021 1544 70
BsrGI T'GTAC_A
Cuts at: 0 1554 4635
Size: 1554 3081
BssSI C'ACGA_G
Cuts at: 0 847 1195 4261 4635
Size: 847 348 3066 374
Fragments arranged by size:
3066 847 374 348
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1800 1989 3722 4635
Size: 1800 189 1733 913
Fragments arranged by size:
1800 1733 913 189
BstEII G'GTnAC_C
Cuts at: 0 3219 4635
Size: 3219 1416
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3628 3976 4362 4635
Size: 3628 348 386 273
Fragments arranged by size:
3628 386 348 273
BstYI r'GATC_y
Cuts at: 0 100 321 925 1364 4109 4635
Size: 100 221 604 439 2745 526
Fragments arranged by size:
2745 604 526 439 221 100
Bsu36I CC'TnA_GG
Cuts at: 0 4194 4635
Size: 4194 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 910 4635
Size: 910 3725
BtsI GCAGTG_nn'
Cuts at: 0 205 1732 1883 4635
Size: 205 1527 151 2752
Fragments arranged by size:
2752 1527 205 151
DraIII CAC_nnn'GTG
Cuts at: 0 2681 4635
Size: 2681 1954
DrdI GACnn_nn'nnGTC
Cuts at: 0 4596 4635
Size: 4596 39
EaeI y'GGCC_r
Cuts at: 0 4470 4500 4635
Size: 4470 30 135
Fragments arranged by size:
4470 135 30
EagI C'GGCC_G
Cuts at: 0 4500 4635
Size: 4500 135
EarI CTCTTCn'nnn_
Cuts at: 0 311 686 1013 1331 1352 1421 2057 3500
Size: 311 375 327 318 21 69 636 1443
Cuts at: 3500 3900 4635
Size: 400 735
Fragments arranged by size:
1443 735 636 400 375 327 318 311
69 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 577 1573 2409 2496 4455 4635
Size: 577 996 836 87 1959 180
Fragments arranged by size:
1959 996 836 577 180 87
EcoICRI GAG'CTC
Cuts at: 0 522 687 1215 2043 2412 4635
Size: 522 165 528 828 369 2223
Fragments arranged by size:
2223 828 528 522 369 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 130 410 479 502 556 640 671
Size: 100 30 280 69 23 54 84 31
Cuts at: 671 847 977 1078 1120 1132 1183 1231 1321
Size: 176 130 101 42 12 51 48 90
Cuts at: 1321 1376 1468 1666 1687 1758 1957 1993 2047
Size: 55 92 198 21 71 199 36 54
Cuts at: 2047 2182 2359 2371 2377 2576 2845 2986 3151
Size: 135 177 12 6 199 269 141 165
Cuts at: 3151 3339 3499 4353 4501 4613 4635
Size: 188 160 854 148 112 22
Fragments arranged by size:
854 280 269 199 199 198 188 177
176 165 160 148 141 135 130 112
101 100 92 90 84 71 69 55
54 54 51 48 42 36 31 30
23 22 21 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 2353 4264 4635
Size: 112 2241 1911 371
Fragments arranged by size:
2241 1911 371 112
EcoO109I rG'GnC_Cy
Cuts at: 0 117 1692 3474 4133 4180 4293 4390 4589
Size: 117 1575 1782 659 47 113 97 199
Cuts at: 4589 4635
Size: 46
Fragments arranged by size:
1782 1575 659 199 117 113 97 47
46
EcoRI G'AATT_C
Cuts at: 0 394 3844 4635
Size: 394 3450 791
Fragments arranged by size:
3450 791 394
EcoRV GAT'ATC
Cuts at: 0 225 4635
Size: 225 4410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 340 4635
Size: 340 4295
FspI TGC'GCA
Cuts at: 0 4453 4635
Size: 4453 182
HaeII r_GCGC'y
Cuts at: 0 1224 1541 3216 4381 4635
Size: 1224 317 1675 1165 254
Fragments arranged by size:
1675 1224 1165 317 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 291 313 323 345 402 434 1246 1278
Size: 291 22 10 22 57 32 812 32
Cuts at: 1278 1379 1411 1626 1658 1747 1779 1821 1853
Size: 101 32 215 32 89 32 42 32
Cuts at: 1853 1890 1922 2401 2433 2963 2995 3126 3158
Size: 37 32 479 32 530 32 131 32
Cuts at: 3158 4635
Size: 1477
Fragments arranged by size:
1477 812 530 479 291 215 131 101
89 57 42 37 32 32 32 32
32 32 32 32 32 32 22 22
10
HincII GTy'rAC
Cuts at: 0 1271 4635
Size: 1271 3364
HindIII A'AGCT_T
Cuts at: 0 2010 4635
Size: 2010 2625
KasI G'GCGC_C
Cuts at: 0 1537 4635
Size: 1537 3098
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 764 1058 3923 4635
Size: 293 471 294 2865 712
Fragments arranged by size:
2865 712 471 294 293
MslI CAynn'nnrTG
Cuts at: 0 1388 2727 4635
Size: 1388 1339 1908
Fragments arranged by size:
1908 1388 1339
MspA1I CmG'CkG
Cuts at: 0 1110 1427 1467 1842 2193 3761 4596 4635
Size: 1110 317 40 375 351 1568 835 39
Fragments arranged by size:
1568 1110 835 375 351 317 40 39
NaeI GCC'GGC
Cuts at: 0 4567 4635
Size: 4567 68
NarI GG'CG_CC
Cuts at: 0 1538 4635
Size: 1538 3097
NgoMIV G'CCGG_C
Cuts at: 0 4565 4635
Size: 4565 70
NheI G'CTAG_C
Cuts at: 0 3186 4635
Size: 3186 1449
NspI r_CATG'y
Cuts at: 0 1987 2628 2670 4635
Size: 1987 641 42 1965
Fragments arranged by size:
1987 1965 641 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 1866 3161 3442 3870 4635
Size: 1866 1295 281 428 765
Fragments arranged by size:
1866 1295 765 428 281
PfoI T'CCnGG_A
Cuts at: 0 107 2963 3848 4635
Size: 107 2856 885 787
Fragments arranged by size:
2856 885 787 107
PmlI CAC'GTG
Cuts at: 0 1655 2771 4635
Size: 1655 1116 1864
Fragments arranged by size:
1864 1655 1116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 4635
Size: 2027 32 2576
Fragments arranged by size:
2576 2027 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 3474 4133 4589 4635
Size: 117 3357 659 456 46
Fragments arranged by size:
3357 659 456 117 46
PspOMI G'GGCC_C
Cuts at: 0 4179 4293 4635
Size: 4179 114 342
Fragments arranged by size:
4179 342 114
PspXI vC'TCGA_Gb
Cuts at: 0 3838 4635
Size: 3838 797
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4156 4188 4635
Size: 4156 32 447
Fragments arranged by size:
4156 447 32
PstI C_TGCA'G
Cuts at: 0 212 581 632 1253 1283 1796 1913 2099
Size: 212 369 51 621 30 513 117 186
Cuts at: 2099 2327 3760 4635
Size: 228 1433 875
Fragments arranged by size:
1433 875 621 513 369 228 212 186
117 51 30
PvuII CAG'CTG
Cuts at: 0 1110 1427 1467 2193 3761 4635
Size: 1110 317 40 726 1568 874
Fragments arranged by size:
1568 1110 874 726 317 40
RsrII CG'GwC_CG
Cuts at: 0 2417 4442 4635
Size: 2417 2025 193
Fragments arranged by size:
2417 2025 193
SacI G_AGCT'C
Cuts at: 0 524 689 1217 2045 2414 4635
Size: 524 165 528 828 369 2221
Fragments arranged by size:
2221 828 528 524 369 165
SanDI GG'GwC_CC
Cuts at: 0 4133 4635
Size: 4133 502
SapI GCTCTTCn'nnn_
Cuts at: 0 1421 2057 3500 4635
Size: 1421 636 1443 1135
Fragments arranged by size:
1443 1421 1135 636
SbfI CC_TGCA'GG
Cuts at: 0 1253 1283 4635
Size: 1253 30 3352
Fragments arranged by size:
3352 1253 30
ScaI AGT'ACT
Cuts at: 0 3955 4635
Size: 3955 680
SexAI A'CCwGG_T
Cuts at: 0 3362 4635
Size: 3362 1273
SfcI C'TryA_G
Cuts at: 0 208 577 628 1237 1249 1279 1792 1909
Size: 208 369 51 609 12 30 513 117
Cuts at: 1909 2095 2323 3756 4056 4635
Size: 186 228 1433 300 579
Fragments arranged by size:
1433 609 579 513 369 300 228 208
186 117 51 30 12
SfoI GGC'GCC
Cuts at: 0 1539 4635
Size: 1539 3096
SmaI CCC'GGG
Cuts at: 0 2425 3168 4536 4635
Size: 2425 743 1368 99
Fragments arranged by size:
2425 1368 743 99
SmlI C'TyrA_G
Cuts at: 0 278 1216 2123 2614 3838 4024 4635
Size: 278 938 907 491 1224 186 611
Fragments arranged by size:
1224 938 907 611 491 278 186
SphI G_CATG'C
Cuts at: 0 1987 2628 4635
Size: 1987 641 2007
Fragments arranged by size:
2007 1987 641
SspI AAT'ATT
Cuts at: 0 2637 4635
Size: 2637 1998
StuI AGG'CCT
Cuts at: 0 85 1735 2827 4635
Size: 85 1650 1092 1808
Fragments arranged by size:
1808 1650 1092 85
StyI C'CwwG_G
Cuts at: 0 2867 3869 4635
Size: 2867 1002 766
Fragments arranged by size:
2867 1002 766
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1486 1741 2474 2480 3606 4347 4459 4635
Size: 1486 255 733 6 1126 741 112 176
Fragments arranged by size:
1486 1126 741 733 255 176 112 6
TatI w'GTAC_w
Cuts at: 0 1429 1554 2327 2954 3830 3953 4635
Size: 1429 125 773 627 876 123 682
Fragments arranged by size:
1429 876 773 682 627 125 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3971 4003 4247 4279 4635
Size: 3971 32 244 32 356
Fragments arranged by size:
3971 356 244 32 32
XbaI T'CTAG_A
Cuts at: 0 1188 2142 3330 4635
Size: 1188 954 1188 1305
Fragments arranged by size:
1305 1188 1188 954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2550 4635
Size: 2550 2085
XhoI C'TCGA_G
Cuts at: 0 3838 4635
Size: 3838 797
XmaI C'CCGG_G
Cuts at: 0 2423 3166 4534 4635
Size: 2423 743 1368 101
Fragments arranged by size:
2423 1368 743 101
XmnI GAAnn'nnTTC
Cuts at: 0 2201 2390 4635
Size: 2201 189 2245
Fragments arranged by size:
2245 2201 189
Enzymes that do cut:
AarI AcuI AfeI AflII AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuII RsrII SacI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SmaI SmlI SphI SspI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AleI AloI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BfrBI
BglII BsaBI BsiWI BsmBI BsrDI BssHII BstBI BstZ17I
BtgI ClaI CspCI DraI FseI FspAI HpaI KpnI
MfeI MluI MscI NcoI NdeI NotI NruI NsiI
PacI PasI PciI PmeI PshAI PsiI PvuI SacII
SalI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI
Tth111I ZraI