Restriction map for
pF1KA0950
(Linear) (Six-base) MAPSORT of: KIAA0950.seq Check: 6160 from: 1 to: 948
KIAA0950.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 948
Size: 48 900
AccI GT'mk_AC
Cuts at: 0 317 948
Size: 317 631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 690 948
Size: 618 72 258
Fragments arranged by size:
618 258 72
AleI CACnn'nnGTG
Cuts at: 0 172 580 948
Size: 172 408 368
Fragments arranged by size:
408 368 172
AlwNI CAG_nnn'CTG
Cuts at: 0 342 825 948
Size: 342 483 123
Fragments arranged by size:
483 342 123
BanI G'GyrC_C
Cuts at: 0 174 948
Size: 174 774
BanII G_rGCy'C
Cuts at: 0 260 856 879 948
Size: 260 596 23 69
Fragments arranged by size:
596 260 69 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 624 948
Size: 624 324
BbvCI CC'TCA_GC
Cuts at: 0 96 948
Size: 96 852
BlpI GC'TnA_GC
Cuts at: 0 850 948
Size: 850 98
Bme1580I G_kGCm'C
Cuts at: 0 95 752 948
Size: 95 657 196
Fragments arranged by size:
657 196 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 432 557 787 813 948
Size: 432 125 230 26 135
Fragments arranged by size:
432 230 135 125 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 204 948
Size: 68 136 744
Fragments arranged by size:
744 136 68
Bpu10I CC'TnA_GC
Cuts at: 0 96 948
Size: 96 852
BsaI GGTCTCn'nnnn_
Cuts at: 0 242 948
Size: 242 706
BsaHI Gr'CG_yC
Cuts at: 0 840 948
Size: 840 108
BsaWI w'CCGG_w
Cuts at: 0 244 948
Size: 244 704
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 646 676 773 803 948
Size: 562 30 54 30 97 30 145
Fragments arranged by size:
562 145 97 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 874 948
Size: 874 74
BseYI C'CCAG_C
Cuts at: 0 159 191 208 275 948
Size: 159 32 17 67 673
Fragments arranged by size:
673 159 67 32 17
BsiHKAI G_wGCw'C
Cuts at: 0 260 680 948
Size: 260 420 268
Fragments arranged by size:
420 268 260
Bsp1286I G_dGCh'C
Cuts at: 0 95 260 680 752 856 879 948
Size: 95 165 420 72 104 23 69
Fragments arranged by size:
420 165 104 95 72 69 23
BspHI T'CATG_A
Cuts at: 0 692 948
Size: 692 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 948
Size: 48 900
BsrBI CCG'CTC
Cuts at: 0 784 948
Size: 784 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 60 433 769 948
Size: 60 373 336 179
Fragments arranged by size:
373 336 179 60
BstEII G'GTnAC_C
Cuts at: 0 345 832 948
Size: 345 487 116
Fragments arranged by size:
487 345 116
Bsu36I CC'TnA_GG
Cuts at: 0 857 948
Size: 857 91
BtgI C'CryG_G
Cuts at: 0 20 533 948
Size: 20 513 415
Fragments arranged by size:
513 415 20
BtsI GCAGTG_nn'
Cuts at: 0 95 948
Size: 95 853
EaeI y'GGCC_r
Cuts at: 0 722 948
Size: 722 226
EarI CTCTTCn'nnn_
Cuts at: 0 265 685 948
Size: 265 420 263
Fragments arranged by size:
420 265 263
EcoICRI GAG'CTC
Cuts at: 0 258 948
Size: 258 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 204 618 690 948
Size: 68 136 414 72 258
Fragments arranged by size:
414 258 136 72 68
EcoO109I rG'GnC_Cy
Cuts at: 0 33 149 948
Size: 33 116 799
Fragments arranged by size:
799 116 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 672 948
Size: 672 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 646 678 948
Size: 290 32 324 32 270
Fragments arranged by size:
324 290 270 32 32
MscI TGG'CCA
Cuts at: 0 724 948
Size: 724 224
MslI CAynn'nnrTG
Cuts at: 0 172 550 580 948
Size: 172 378 30 368
Fragments arranged by size:
378 368 172 30
MspA1I CmG'CkG
Cuts at: 0 172 275 339 710 948
Size: 172 103 64 371 238
Fragments arranged by size:
371 238 172 103 64
NcoI C'CATG_G
Cuts at: 0 533 948
Size: 533 415
PasI CC'CwG_GG
Cuts at: 0 6 37 948
Size: 6 31 911
Fragments arranged by size:
911 31 6
PstI C_TGCA'G
Cuts at: 0 926 948
Size: 926 22
PvuII CAG'CTG
Cuts at: 0 275 339 948
Size: 275 64 609
Fragments arranged by size:
609 275 64
SacI G_AGCT'C
Cuts at: 0 260 948
Size: 260 688
SapI GCTCTTCn'nnn_
Cuts at: 0 265 685 948
Size: 265 420 263
Fragments arranged by size:
420 265 263
SfcI C'TryA_G
Cuts at: 0 922 948
Size: 922 26
StyI C'CwwG_G
Cuts at: 0 350 533 948
Size: 350 183 415
Fragments arranged by size:
415 350 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 832 948
Size: 58 774 116
Fragments arranged by size:
774 116 58
Tth111I GACn'n_nGTC
Cuts at: 0 385 397 948
Size: 385 12 551
Fragments arranged by size:
551 385 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 419 948
Size: 419 529
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI BanI BanII BbsI
BbvCI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BstAPI BstEII Bsu36I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I
NcoI PasI PstI PvuII SacI SapI SfcI StyI
TaqII Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XbaI XhoI XmaI XmnI
ZraI