Restriction map for
pF1KA0953
(Linear) (Six-base) MAPSORT of: KIAA0953.seq Check: 9832 from: 1 to: 2184
KIAA0953.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2091 2184
Size: 2091 93
AccI GT'mk_AC
Cuts at: 0 1757 2184
Size: 1757 427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 2184
Size: 1528 656
AfeI AGC'GCT
Cuts at: 0 912 1180 2184
Size: 912 268 1004
Fragments arranged by size:
1004 912 268
AflIII A'CryG_T
Cuts at: 0 215 2184
Size: 215 1969
AhdI GACnn_n'nnGTC
Cuts at: 0 396 2184
Size: 396 1788
AleI CACnn'nnGTG
Cuts at: 0 1822 2184
Size: 1822 362
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 2184
Size: 1102 32 1050
Fragments arranged by size:
1102 1050 32
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1828 2184
Size: 882 946 356
Fragments arranged by size:
946 882 356
ApaI G_GGCC'C
Cuts at: 0 989 2184
Size: 989 1195
ApoI r'AATT_y
Cuts at: 0 485 1406 2184
Size: 485 921 778
Fragments arranged by size:
921 778 485
AvaI C'yCGr_G
Cuts at: 0 79 453 1894 2184
Size: 79 374 1441 290
Fragments arranged by size:
1441 374 290 79
BamHI G'GATC_C
Cuts at: 0 75 2184
Size: 75 2109
BanI G'GyrC_C
Cuts at: 0 25 160 352 1084 2184
Size: 25 135 192 732 1100
Fragments arranged by size:
1100 732 192 135 25
BanII G_rGCy'C
Cuts at: 0 682 989 1150 1958 2184
Size: 682 307 161 808 226
Fragments arranged by size:
808 682 307 226 161
BbeI G_GCGC'C
Cuts at: 0 164 2184
Size: 164 2020
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 535 697 1539 2184
Size: 103 432 162 842 645
Fragments arranged by size:
842 645 432 162 103
BbvCI CC'TCA_GC
Cuts at: 0 1018 2039 2184
Size: 1018 1021 145
Fragments arranged by size:
1021 1018 145
BciVI GTATCCnnnnn_n'
Cuts at: 0 1323 2184
Size: 1323 861
BlpI GC'TnA_GC
Cuts at: 0 1033 2184
Size: 1033 1151
Bme1580I G_kGCm'C
Cuts at: 0 30 989 1773 2184
Size: 30 959 784 411
Fragments arranged by size:
959 784 411 30
BmgBI CAC'GTC
Cuts at: 0 192 1725 2184
Size: 192 1533 459
Fragments arranged by size:
1533 459 192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 656 2109 2184
Size: 656 1453 75
Fragments arranged by size:
1453 656 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 340 1018 2184
Size: 122 218 678 1166
Fragments arranged by size:
1166 678 218 122
Bpu10I CC'TnA_GC
Cuts at: 0 1018 1591 2039 2184
Size: 1018 573 448 145
Fragments arranged by size:
1018 573 448 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 2184
Size: 283 1901
BsaI GGTCTCn'nnnn_
Cuts at: 0 644 1855 2080 2184
Size: 644 1211 225 104
Fragments arranged by size:
1211 644 225 104
BsaAI yAC'GTr
Cuts at: 0 216 2184
Size: 216 1968
BsaBI GATnn'nnATC
Cuts at: 0 1432 2054 2184
Size: 1432 622 130
Fragments arranged by size:
1432 622 130
BsaHI Gr'CG_yC
Cuts at: 0 161 896 2184
Size: 161 735 1288
Fragments arranged by size:
1288 735 161
BsaWI w'CCGG_w
Cuts at: 0 407 2127 2184
Size: 407 1720 57
Fragments arranged by size:
1720 407 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1102 1132 1165 1195 2184
Size: 1102 30 33 30 989
Fragments arranged by size:
1102 989 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 637 1123 1693 1756 1948 2085 2088
Size: 400 237 486 570 63 192 137 3
Cuts at: 2088 2152 2184
Size: 64 32
Fragments arranged by size:
570 486 400 237 192 137 64 63
32 3
BseYI C'CCAG_C
Cuts at: 0 717 1565 2184
Size: 717 848 619
Fragments arranged by size:
848 717 619
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1636 2184
Size: 1213 423 548
Fragments arranged by size:
1213 548 423
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1531 1958 2184
Size: 1106 425 427 226
Fragments arranged by size:
1106 427 425 226
Bsp1286I G_dGCh'C
Cuts at: 0 30 682 989 1106 1150 1531 1773 1958
Size: 30 652 307 117 44 381 242 185
Cuts at: 1958 2184
Size: 226
Fragments arranged by size:
652 381 307 242 226 185 117 44
30
BspEI T'CCGG_A
Cuts at: 0 2127 2184
Size: 2127 57
BspHI T'CATG_A
Cuts at: 0 1202 1550 2184
Size: 1202 348 634
Fragments arranged by size:
1202 634 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 1299 2091 2184
Size: 347 952 792 93
Fragments arranged by size:
952 792 347 93
BsrDI GCAATG_nn'
Cuts at: 0 221 2184
Size: 221 1963
BsrFI r'CCGG_y
Cuts at: 0 635 973 2015 2184
Size: 635 338 1042 169
Fragments arranged by size:
1042 635 338 169
BssHII G'CGCG_C
Cuts at: 0 924 2184
Size: 924 1260
BssSI C'ACGA_G
Cuts at: 0 1105 2184
Size: 1105 1079
BstBI TT'CG_AA
Cuts at: 0 489 2184
Size: 489 1695
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 318 996 2163 2184
Size: 208 110 678 1167 21
Fragments arranged by size:
1167 678 208 110 21
BstYI r'GATC_y
Cuts at: 0 75 344 2184
Size: 75 269 1840
Fragments arranged by size:
1840 269 75
Bsu36I CC'TnA_GG
Cuts at: 0 71 2184
Size: 71 2113
BtgI C'CryG_G
Cuts at: 0 683 956 1142 1216 2184
Size: 683 273 186 74 968
Fragments arranged by size:
968 683 273 186 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 948 2184
Size: 948 1236
BtsI GCAGTG_nn'
Cuts at: 0 261 595 2004 2184
Size: 261 334 1409 180
Fragments arranged by size:
1409 334 261 180
ClaI AT'CG_AT
Cuts at: 0 204 2184
Size: 204 1980
DraI TTT'AAA
Cuts at: 0 825 2184
Size: 825 1359
DrdI GACnn_nn'nnGTC
Cuts at: 0 1072 1501 2184
Size: 1072 429 683
Fragments arranged by size:
1072 683 429
EaeI y'GGCC_r
Cuts at: 0 308 1219 2184
Size: 308 911 965
Fragments arranged by size:
965 911 308
EarI CTCTTCn'nnn_
Cuts at: 0 286 1201 1966 2184
Size: 286 915 765 218
Fragments arranged by size:
915 765 286 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1954 2184
Size: 1954 230
EcoICRI GAG'CTC
Cuts at: 0 1956 2184
Size: 1956 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 340 1018 1528 2184
Size: 122 218 678 510 656
Fragments arranged by size:
678 656 510 218 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 1306 1393 2184
Size: 1231 75 87 791
Fragments arranged by size:
1231 791 87 75
EcoO109I rG'GnC_Cy
Cuts at: 0 564 985 1212 2184
Size: 564 421 227 972
Fragments arranged by size:
972 564 421 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 2184
Size: 135 2049
HaeII r_GCGC'y
Cuts at: 0 164 914 1182 1636 2184
Size: 164 750 268 454 548
Fragments arranged by size:
750 548 454 268 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 622 654 1513 1545 2047 2075
Size: 382 32 208 32 859 32 502 28
Cuts at: 2075 2079 2098 2107 2109 2130 2141 2184
Size: 4 19 9 2 21 11 43
Fragments arranged by size:
859 502 382 208 43 32 32 32
28 21 19 11 9 4 2
HincII GTy'rAC
Cuts at: 0 57 2184
Size: 57 2127
KasI G'GCGC_C
Cuts at: 0 160 2184
Size: 160 2024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 2184
Size: 929 1255
MscI TGG'CCA
Cuts at: 0 310 2184
Size: 310 1874
MslI CAynn'nnrTG
Cuts at: 0 206 226 1822 1847 2184
Size: 206 20 1596 25 337
Fragments arranged by size:
1596 337 206 25 20
MspA1I CmG'CkG
Cuts at: 0 246 685 958 1026 1218 1766 2043 2184
Size: 246 439 273 68 192 548 277 141
Fragments arranged by size:
548 439 277 273 246 192 141 68
NarI GG'CG_CC
Cuts at: 0 161 2184
Size: 161 2023
NspI r_CATG'y
Cuts at: 0 619 1119 1890 2184
Size: 619 500 771 294
Fragments arranged by size:
771 619 500 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 560 1239 2184
Size: 560 679 945
Fragments arranged by size:
945 679 560
PfoI T'CCnGG_A
Cuts at: 0 1121 1364 2184
Size: 1121 243 820
Fragments arranged by size:
1121 820 243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 2184
Size: 1102 32 1050
Fragments arranged by size:
1102 1050 32
PpuMI rG'GwC_Cy
Cuts at: 0 564 1212 2184
Size: 564 648 972
Fragments arranged by size:
972 648 564
PshAI GACnn'nnGTC
Cuts at: 0 194 2184
Size: 194 1990
PspOMI G'GGCC_C
Cuts at: 0 985 2184
Size: 985 1199
PspXI vC'TCGA_Gb
Cuts at: 0 453 2184
Size: 453 1731
PstI C_TGCA'G
Cuts at: 0 344 551 1310 2102 2184
Size: 344 207 759 792 82
Fragments arranged by size:
792 759 344 207 82
PvuII CAG'CTG
Cuts at: 0 246 1026 2043 2184
Size: 246 780 1017 141
Fragments arranged by size:
1017 780 246 141
RsrII CG'GwC_CG
Cuts at: 0 2013 2184
Size: 2013 171
SacI G_AGCT'C
Cuts at: 0 1958 2184
Size: 1958 226
SacII CC_GC'GG
Cuts at: 0 686 959 1219 2184
Size: 686 273 260 965
Fragments arranged by size:
965 686 273 260
SanDI GG'GwC_CC
Cuts at: 0 564 2184
Size: 564 1620
SbfI CC_TGCA'GG
Cuts at: 0 1310 2102 2184
Size: 1310 792 82
Fragments arranged by size:
1310 792 82
ScaI AGT'ACT
Cuts at: 0 995 2184
Size: 995 1189
SexAI A'CCwGG_T
Cuts at: 0 863 2184
Size: 863 1321
SfcI C'TryA_G
Cuts at: 0 340 547 610 1306 1575 2098 2184
Size: 340 207 63 696 269 523 86
Fragments arranged by size:
696 523 340 269 207 86 63
SfoI GGC'GCC
Cuts at: 0 162 2184
Size: 162 2022
SmlI C'TyrA_G
Cuts at: 0 298 453 2184
Size: 298 155 1731
Fragments arranged by size:
1731 298 155
StuI AGG'CCT
Cuts at: 0 509 2184
Size: 509 1675
StyI C'CwwG_G
Cuts at: 0 800 2184
Size: 800 1384
TatI w'GTAC_w
Cuts at: 0 836 993 2184
Size: 836 157 1191
Fragments arranged by size:
1191 836 157
Tth111I GACn'n_nGTC
Cuts at: 0 1543 2184
Size: 1543 641
XbaI T'CTAG_A
Cuts at: 0 1422 2184
Size: 1422 762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 1389 2184
Size: 978 411 795
Fragments arranged by size:
978 795 411
XhoI C'TCGA_G
Cuts at: 0 453 2184
Size: 453 1731
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NspI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII RsrII SacI SacII SanDI SbfI ScaI SexAI
SfcI SfoI SmlI StuI StyI TatI Tth111I XbaI
XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BcgI BclI BfrBI BglI
BglII BmrI BmtI BsiEI BsiWI BsmI BsmBI BsrBI
BsrGI BstAPI BstEII BstZ17I CspCI DraIII EagI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PsiI PsrI
PvuI SalI SapI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI XmaI
XmnI ZraI