Restriction map for
pF1KA0954
(Linear) (Six-base) MAPSORT of: KIAA0954.seq Check: 2834 from: 1 to: 3453
KIAA0954.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 441 3453
Size: 441 3012
AccI GT'mk_AC
Cuts at: 0 2393 3453
Size: 2393 1060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1425 2154 2316 3453
Size: 1312 113 729 162 1137
Fragments arranged by size:
1312 1137 729 162 113
AfeI AGC'GCT
Cuts at: 0 34 2101 2287 3453
Size: 34 2067 186 1166
Fragments arranged by size:
2067 1166 186 34
AflIII A'CryG_T
Cuts at: 0 1779 2624 3453
Size: 1779 845 829
Fragments arranged by size:
1779 845 829
AgeI A'CCGG_T
Cuts at: 0 3311 3453
Size: 3311 142
AleI CACnn'nnGTG
Cuts at: 0 1417 3453
Size: 1417 2036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1600 3181 3453
Size: 1600 1581 272
Fragments arranged by size:
1600 1581 272
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3184 3216 3453
Size: 3184 32 237
Fragments arranged by size:
3184 237 32
AlwNI CAG_nnn'CTG
Cuts at: 0 666 708 720 810 2605 3063 3453
Size: 666 42 12 90 1795 458 390
Fragments arranged by size:
1795 666 458 390 90 42 12
ApaI G_GGCC'C
Cuts at: 0 359 919 2952 2964 2983 3003 3453
Size: 359 560 2033 12 19 20 450
Fragments arranged by size:
2033 560 450 359 20 19 12
ApaLI G'TGCA_C
Cuts at: 0 1717 1837 3453
Size: 1717 120 1616
Fragments arranged by size:
1717 1616 120
ApoI r'AATT_y
Cuts at: 0 3069 3343 3453
Size: 3069 274 110
Fragments arranged by size:
3069 274 110
AvaI C'yCGr_G
Cuts at: 0 249 276 342 423 869 912 1258 1855
Size: 249 27 66 81 446 43 346 597
Cuts at: 1855 2160 3453
Size: 305 1293
Fragments arranged by size:
1293 597 446 346 305 249 81 66
43 27
BanI G'GyrC_C
Cuts at: 0 61 254 286 349 669 965 976 1647
Size: 61 193 32 63 320 296 11 671
Cuts at: 1647 2164 3157 3301 3453
Size: 517 993 144 152
Fragments arranged by size:
993 671 517 320 296 193 152 144
63 61 32 11
BanII G_rGCy'C
Cuts at: 0 74 229 359 377 430 464 632 743
Size: 74 155 130 18 53 34 168 111
Cuts at: 743 876 919 1241 1744 2952 2964 2983 2993
Size: 133 43 322 503 1208 12 19 10
Cuts at: 2993 3003 3453
Size: 10 450
Fragments arranged by size:
1208 503 450 322 168 155 133 130
111 74 53 43 34 19 18 12
10 10
BbeI G_GCGC'C
Cuts at: 0 258 2168 3453
Size: 258 1910 1285
Fragments arranged by size:
1910 1285 258
BbsI GAAGACnn'nnnn_
Cuts at: 0 1653 3453
Size: 1653 1800
BbvCI CC'TCA_GC
Cuts at: 0 3172 3453
Size: 3172 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 884 3453
Size: 850 34 2569
Fragments arranged by size:
2569 850 34
BclI T'GATC_A
Cuts at: 0 821 3453
Size: 821 2632
BglI GCCn_nnn'nGGC
Cuts at: 0 166 888 1019 1525 1679 3453
Size: 166 722 131 506 154 1774
Fragments arranged by size:
1774 722 506 166 154 131
BglII A'GATC_T
Cuts at: 0 1565 1928 3453
Size: 1565 363 1525
Fragments arranged by size:
1565 1525 363
BlpI GC'TnA_GC
Cuts at: 0 371 737 2365 2987 3453
Size: 371 366 1628 622 466
Fragments arranged by size:
1628 622 466 371 366
Bme1580I G_kGCm'C
Cuts at: 0 66 352 359 515 674 919 981 1644
Size: 66 286 7 156 159 245 62 663
Cuts at: 1644 1652 1721 1841 2482 2952 2964 2983 3003
Size: 8 69 120 641 470 12 19 20
Cuts at: 3003 3041 3304 3453
Size: 38 263 149
Fragments arranged by size:
663 641 470 286 263 245 159 156
149 120 69 66 62 38 20 19
12 8 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1344 3084 3453
Size: 1344 1740 369
Fragments arranged by size:
1740 1344 369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 456 757 2980 3453
Size: 141 315 301 2223 473
Fragments arranged by size:
2223 473 315 301 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 118 140 189 527 544 1333 1507
Size: 88 30 22 49 338 17 789 174
Cuts at: 1507 2095 2116 2124 2884 2950 3085 3086 3453
Size: 588 21 8 760 66 135 1 367
Fragments arranged by size:
789 760 588 367 338 174 135 88
66 49 30 22 21 17 8 1
Bpu10I CC'TnA_GC
Cuts at: 0 223 1403 1913 2760 3172 3453
Size: 223 1180 510 847 412 281
Fragments arranged by size:
1180 847 510 412 281 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 2128 3453
Size: 1252 876 1325
Fragments arranged by size:
1325 1252 876
BsaI GGTCTCn'nnnn_
Cuts at: 0 1441 3453
Size: 1441 2012
BsaAI yAC'GTr
Cuts at: 0 504 1782 2361 3453
Size: 504 1278 579 1092
Fragments arranged by size:
1278 1092 579 504
BsaBI GATnn'nnATC
Cuts at: 0 3079 3453
Size: 3079 374
BsaHI Gr'CG_yC
Cuts at: 0 255 1175 1255 2165 3453
Size: 255 920 80 910 1288
Fragments arranged by size:
1288 920 910 255 80
BsaWI w'CCGG_w
Cuts at: 0 1358 1954 2262 3311 3453
Size: 1358 596 308 1049 142
Fragments arranged by size:
1358 1049 596 308 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 1949 1979 3184 3214 3260 3290
Size: 412 30 1507 30 1205 30 46 30
Cuts at: 3290 3453
Size: 163
Fragments arranged by size:
1507 1205 412 163 46 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 2395 3186 3189 3268 3453
Size: 115 2280 791 3 79 185
Fragments arranged by size:
2280 791 185 115 79 3
BseYI C'CCAG_C
Cuts at: 0 446 692 787 1230 2450 2704 2740 2746
Size: 446 246 95 443 1220 254 36 6
Cuts at: 2746 2767 2926 3372 3407 3453
Size: 21 159 446 35 46
Fragments arranged by size:
1220 446 446 443 254 246 159 95
46 36 35 21 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 844 1282 1420 1804 3151 3243 3453
Size: 694 150 438 138 384 1347 92 210
Fragments arranged by size:
1347 694 438 384 210 150 138 92
BsiEI CG_ry'CG
Cuts at: 0 1674 1699 3453
Size: 1674 25 1754
Fragments arranged by size:
1754 1674 25
BsiHKAI G_wGCw'C
Cuts at: 0 743 1721 1841 3102 3453
Size: 743 978 120 1261 351
Fragments arranged by size:
1261 978 743 351 120
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 2071 3453
Size: 10 2061 1382
Fragments arranged by size:
2061 1382 10
Bsp1286I G_dGCh'C
Cuts at: 0 66 74 229 352 359 377 430 464
Size: 66 8 155 123 7 18 53 34
Cuts at: 464 515 632 674 743 876 919 981 1241
Size: 51 117 42 69 133 43 62 260
Cuts at: 1241 1644 1652 1721 1744 1841 2482 2952 2964
Size: 403 8 69 23 97 641 470 12
Cuts at: 2964 2983 2993 3003 3041 3102 3304 3453
Size: 19 10 10 38 61 202 149
Fragments arranged by size:
641 470 403 260 202 155 149 133
123 117 97 69 69 66 62 61
53 51 43 42 38 34 23 19
18 12 10 10 8 8 7
BspEI T'CCGG_A
Cuts at: 0 2262 3453
Size: 2262 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 1398 1772 3453
Size: 441 957 374 1681
Fragments arranged by size:
1681 957 441 374
BsrBI CCG'CTC
Cuts at: 0 906 2759 3453
Size: 906 1853 694
Fragments arranged by size:
1853 906 694
BsrDI GCAATG_nn'
Cuts at: 0 938 2306 3453
Size: 938 1368 1147
Fragments arranged by size:
1368 1147 938
BsrFI r'CCGG_y
Cuts at: 0 1694 2152 3311 3453
Size: 1694 458 1159 142
Fragments arranged by size:
1694 1159 458 142
BsrGI T'GTAC_A
Cuts at: 0 614 1217 3453
Size: 614 603 2236
Fragments arranged by size:
2236 614 603
BssHII G'CGCG_C
Cuts at: 0 1767 3453
Size: 1767 1686
BstBI TT'CG_AA
Cuts at: 0 41 3453
Size: 41 3412
BstEII G'GTnAC_C
Cuts at: 0 2571 3453
Size: 2571 882
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 2458 2500 2775 3453
Size: 96 2362 42 275 678
Fragments arranged by size:
2362 678 275 96 42
BstYI r'GATC_y
Cuts at: 0 609 1565 1817 1928 3453
Size: 609 956 252 111 1525
Fragments arranged by size:
1525 956 609 252 111
Bsu36I CC'TnA_GG
Cuts at: 0 1937 3229 3453
Size: 1937 1292 224
Fragments arranged by size:
1937 1292 224
BtgI C'CryG_G
Cuts at: 0 180 258 653 1668 2255 2482 2534 2940
Size: 180 78 395 1015 587 227 52 406
Cuts at: 2940 3453
Size: 513
Fragments arranged by size:
1015 587 513 406 395 227 180 78
52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 208 863 1637 1893 3453
Size: 208 655 774 256 1560
Fragments arranged by size:
1560 774 655 256 208
BtsI GCAGTG_nn'
Cuts at: 0 400 3163 3367 3453
Size: 400 2763 204 86
Fragments arranged by size:
2763 400 204 86
DraIII CAC_nnn'GTG
Cuts at: 0 1305 3453
Size: 1305 2148
EaeI y'GGCC_r
Cuts at: 0 1556 1671 1696 2117 2237 2718 2838 2873
Size: 1556 115 25 421 120 481 120 35
Cuts at: 2873 2943 3316 3453
Size: 70 373 137
Fragments arranged by size:
1556 481 421 373 137 120 120 115
70 35 25
EagI C'GGCC_G
Cuts at: 0 1671 1696 3453
Size: 1671 25 1757
Fragments arranged by size:
1757 1671 25
EarI CTCTTCn'nnn_
Cuts at: 0 757 1807 2215 3453
Size: 757 1050 408 1238
Fragments arranged by size:
1238 1050 757 408
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 521 3453
Size: 521 2932
EcoICRI GAG'CTC
Cuts at: 0 741 3453
Size: 741 2712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 118 140 189 527 544 1312 1333
Size: 88 30 22 49 338 17 768 21
Cuts at: 1333 1425 1507 2095 2116 2124 2154 2316 2884
Size: 92 82 588 21 8 30 162 568
Cuts at: 2884 2950 3085 3086 3453
Size: 66 135 1 367
Fragments arranged by size:
768 588 568 367 338 162 135 92
88 82 66 49 30 30 22 21
21 17 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 3453
Size: 997 2456
EcoO109I rG'GnC_Cy
Cuts at: 0 356 559 916 1263 1317 2225 2385 2764
Size: 356 203 357 347 54 908 160 379
Cuts at: 2764 2960 2961 2980 3453
Size: 196 1 19 473
Fragments arranged by size:
908 473 379 357 356 347 203 196
160 54 19 1
EcoRI G'AATT_C
Cuts at: 0 3069 3343 3453
Size: 3069 274 110
Fragments arranged by size:
3069 274 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1218 1389 1947 3453
Size: 1218 171 558 1506
Fragments arranged by size:
1506 1218 558 171
FspI TGC'GCA
Cuts at: 0 475 2374 3453
Size: 475 1899 1079
Fragments arranged by size:
1899 1079 475
HaeII r_GCGC'y
Cuts at: 0 28 36 258 326 1711 2103 2168 2289
Size: 28 8 222 68 1385 392 65 121
Cuts at: 2289 3453
Size: 1164
Fragments arranged by size:
1385 1164 392 222 121 68 65 28
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1949 1981 3453
Size: 412 32 1505 32 1472
Fragments arranged by size:
1505 1472 412 32 32
KasI G'GCGC_C
Cuts at: 0 254 2164 3453
Size: 254 1910 1289
Fragments arranged by size:
1910 1289 254
MfeI C'AATT_G
Cuts at: 0 1807 3453
Size: 1807 1646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 152 1619 1784 2541 3453
Size: 101 51 1467 165 757 912
Fragments arranged by size:
1467 912 757 165 101 51
MscI TGG'CCA
Cuts at: 0 2119 2239 2720 2840 2875 3318 3453
Size: 2119 120 481 120 35 443 135
Fragments arranged by size:
2119 481 443 135 120 120 35
MslI CAynn'nnrTG
Cuts at: 0 1225 1417 1510 1582 1724 2023 3058 3453
Size: 1225 192 93 72 142 299 1035 395
Fragments arranged by size:
1225 1035 395 299 192 142 93 72
MspA1I CmG'CkG
Cuts at: 0 209 294 606 1638 3453
Size: 209 85 312 1032 1815
Fragments arranged by size:
1815 1032 312 209 85
NarI GG'CG_CC
Cuts at: 0 255 2165 3453
Size: 255 1910 1288
Fragments arranged by size:
1910 1288 255
NcoI C'CATG_G
Cuts at: 0 258 653 2482 2940 3453
Size: 258 395 1829 458 513
Fragments arranged by size:
1829 513 458 395 258
NspI r_CATG'y
Cuts at: 0 1230 1467 1830 2628 3453
Size: 1230 237 363 798 825
Fragments arranged by size:
1230 825 798 363 237
PasI CC'CwG_GG
Cuts at: 0 232 897 2458 2896 3003 3297 3453
Size: 232 665 1561 438 107 294 156
Fragments arranged by size:
1561 665 438 294 232 156 107
PciI A'CATG_T
Cuts at: 0 2624 3453
Size: 2624 829
PflMI CCAn_nnn'nTGG
Cuts at: 0 1055 2115 2813 3063 3453
Size: 1055 1060 698 250 390
Fragments arranged by size:
1060 1055 698 390 250
PfoI T'CCnGG_A
Cuts at: 0 95 3453
Size: 95 3358
PmlI CAC'GTG
Cuts at: 0 504 1782 3453
Size: 504 1278 1671
Fragments arranged by size:
1671 1278 504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3184 3216 3453
Size: 3184 32 237
Fragments arranged by size:
3184 237 32
PpuMI rG'GwC_Cy
Cuts at: 0 2385 3453
Size: 2385 1068
PshAI GACnn'nnGTC
Cuts at: 0 1903 3453
Size: 1903 1550
PspOMI G'GGCC_C
Cuts at: 0 355 915 2948 2960 2979 2999 3453
Size: 355 560 2033 12 19 20 454
Fragments arranged by size:
2033 560 454 355 20 19 12
PspXI vC'TCGA_Gb
Cuts at: 0 869 3453
Size: 869 2584
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 3118 3150 3453
Size: 1531 32 1555 32 303
Fragments arranged by size:
1555 1531 303 32 32
PstI C_TGCA'G
Cuts at: 0 32 605 725 1001 2297 2886 3118 3188
Size: 32 573 120 276 1296 589 232 70
Cuts at: 3188 3391 3453
Size: 203 62
Fragments arranged by size:
1296 589 573 276 232 203 120 70
62 32
SacI G_AGCT'C
Cuts at: 0 743 3453
Size: 743 2710
SbfI CC_TGCA'GG
Cuts at: 0 1001 2297 3118 3453
Size: 1001 1296 821 335
Fragments arranged by size:
1296 1001 821 335
ScaI AGT'ACT
Cuts at: 0 3094 3453
Size: 3094 359
SexAI A'CCwGG_T
Cuts at: 0 2387 3453
Size: 2387 1066
SfcI C'TryA_G
Cuts at: 0 28 601 721 997 1842 2293 2882 3114
Size: 28 573 120 276 845 451 589 232
Cuts at: 3114 3184 3387 3453
Size: 70 203 66
Fragments arranged by size:
845 589 573 451 276 232 203 120
70 66 28
SfoI GGC'GCC
Cuts at: 0 256 2166 3453
Size: 256 1910 1287
Fragments arranged by size:
1910 1287 256
SgrAI Cr'CCGG_yG
Cuts at: 0 3311 3453
Size: 3311 142
SmaI CCC'GGG
Cuts at: 0 251 278 344 914 3453
Size: 251 27 66 570 2539
Fragments arranged by size:
2539 570 251 66 27
SmlI C'TyrA_G
Cuts at: 0 869 1267 2143 3453
Size: 869 398 876 1310
Fragments arranged by size:
1310 876 869 398
SphI G_CATG'C
Cuts at: 0 1230 1830 3453
Size: 1230 600 1623
Fragments arranged by size:
1623 1230 600
StuI AGG'CCT
Cuts at: 0 436 992 2272 2473 3120 3453
Size: 436 556 1280 201 647 333
Fragments arranged by size:
1280 647 556 436 333 201
StyI C'CwwG_G
Cuts at: 0 258 377 653 961 1772 2231 2482 2618
Size: 258 119 276 308 811 459 251 136
Cuts at: 2618 2931 2940 3453
Size: 313 9 513
Fragments arranged by size:
811 513 459 313 308 276 258 251
136 119 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 2682 3298 3310 3453
Size: 706 1976 616 12 143
Fragments arranged by size:
1976 706 616 143 12
TatI w'GTAC_w
Cuts at: 0 104 614 1217 3092 3453
Size: 104 510 603 1875 361
Fragments arranged by size:
1875 603 510 361 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 3260 3292 3453
Size: 763 32 2465 32 161
Fragments arranged by size:
2465 763 161 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1657 1963 3453
Size: 1657 306 1490
Fragments arranged by size:
1657 1490 306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 811 884 2310 3060 3453
Size: 811 73 1426 750 393
Fragments arranged by size:
1426 811 750 393 73
XhoI C'TCGA_G
Cuts at: 0 869 3453
Size: 869 2584
XmaI C'CCGG_G
Cuts at: 0 249 276 342 912 3453
Size: 249 27 66 570 2541
Fragments arranged by size:
2541 570 249 66 27
XmnI GAAnn'nnTTC
Cuts at: 0 1201 3453
Size: 1201 2252
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI SacI SbfI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BfrBI BmgBI BmtI BsiWI
BsmI BspHI BssSI BstAPI BstZ17I ClaI CspCI DraI
DrdI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PsiI PvuI PvuII RsrII SacII SalI
SanDI SapI SfiI SgfI SnaBI SpeI SrfI SspI
SwaI XbaI ZraI