Restriction map for
pF1KSDA0955
(Linear) (Six-base) MAPSORT of: KIAA0955.seq Check: 7118 from: 1 to: 1293
KIAA0955.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AatII G_ACGT'C
Cuts at: 0 470 1293
Size: 470 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1218 1293
Size: 197 1021 75
Fragments arranged by size:
1021 197 75
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 1293
Size: 604 689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 778 810 1293
Size: 778 32 483
Fragments arranged by size:
778 483 32
AlwNI CAG_nnn'CTG
Cuts at: 0 168 336 369 1293
Size: 168 168 33 924
Fragments arranged by size:
924 168 168 33
ApoI r'AATT_y
Cuts at: 0 875 892 1287 1293
Size: 875 17 395 6
Fragments arranged by size:
875 395 17 6
AvaI C'yCGr_G
Cuts at: 0 527 1293
Size: 527 766
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 959 992 1293
Size: 959 33 301
Fragments arranged by size:
959 301 33
BanII G_rGCy'C
Cuts at: 0 868 1293
Size: 868 425
BciVI GTATCCnnnnn_n'
Cuts at: 0 960 1293
Size: 960 333
BlpI GC'TnA_GC
Cuts at: 0 1189 1293
Size: 1189 104
BmgBI CAC'GTC
Cuts at: 0 1227 1293
Size: 1227 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 535 890 976 1090 1293
Size: 345 190 355 86 114 203
Fragments arranged by size:
355 345 203 190 114 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1293
Size: 949 344
BsaBI GATnn'nnATC
Cuts at: 0 580 1293
Size: 580 713
BsaHI Gr'CG_yC
Cuts at: 0 467 701 1293
Size: 467 234 592
Fragments arranged by size:
592 467 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 1293
Size: 430 30 833
Fragments arranged by size:
833 430 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 1007 1293
Size: 424 583 286
Fragments arranged by size:
583 424 286
BseYI C'CCAG_C
Cuts at: 0 562 694 901 1293
Size: 562 132 207 392
Fragments arranged by size:
562 392 207 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1293
Size: 1052 241
BsiHKAI G_wGCw'C
Cuts at: 0 214 1097 1232 1293
Size: 214 883 135 61
Fragments arranged by size:
883 214 135 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 474 1293
Size: 474 819
Bsp1286I G_dGCh'C
Cuts at: 0 214 868 1097 1232 1293
Size: 214 654 229 135 61
Fragments arranged by size:
654 229 214 135 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 1293
Size: 356 937
BsrFI r'CCGG_y
Cuts at: 0 1055 1293
Size: 1055 238
BssSI C'ACGA_G
Cuts at: 0 1264 1293
Size: 1264 29
BstYI r'GATC_y
Cuts at: 0 987 1293
Size: 987 306
BtgI C'CryG_G
Cuts at: 0 744 1293
Size: 744 549
BtsI GCAGTG_nn'
Cuts at: 0 239 395 1293
Size: 239 156 898
Fragments arranged by size:
898 239 156
EarI CTCTTCn'nnn_
Cuts at: 0 86 1237 1293
Size: 86 1151 56
Fragments arranged by size:
1151 86 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 345 535 890 976 1090 1218 1293
Size: 197 148 190 355 86 114 128 75
Fragments arranged by size:
355 197 190 148 128 114 86 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1025 1293
Size: 1025 268
EcoO109I rG'GnC_Cy
Cuts at: 0 172 1080 1212 1254 1293
Size: 172 908 132 42 39
Fragments arranged by size:
908 172 132 42 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 696 1274 1293
Size: 696 578 19
Fragments arranged by size:
696 578 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 633 665 1293
Size: 70 32 531 32 628
Fragments arranged by size:
628 531 70 32 32
HincII GTy'rAC
Cuts at: 0 51 1293
Size: 51 1242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1293
Size: 656 637
NcoI C'CATG_G
Cuts at: 0 744 1293
Size: 744 549
PfoI T'CCnGG_A
Cuts at: 0 322 512 551 1106 1293
Size: 322 190 39 555 187
Fragments arranged by size:
555 322 190 187 39
PpuMI rG'GwC_Cy
Cuts at: 0 1080 1212 1254 1293
Size: 1080 132 42 39
Fragments arranged by size:
1080 132 42 39
PsiI TTA'TAA
Cuts at: 0 152 1293
Size: 152 1141
PstI C_TGCA'G
Cuts at: 0 353 402 761 1293
Size: 353 49 359 532
Fragments arranged by size:
532 359 353 49
SanDI GG'GwC_CC
Cuts at: 0 1254 1293
Size: 1254 39
SapI GCTCTTCn'nnn_
Cuts at: 0 1237 1293
Size: 1237 56
SfcI C'TryA_G
Cuts at: 0 349 398 757 858 1293
Size: 349 49 359 101 435
Fragments arranged by size:
435 359 349 101 49
SmaI CCC'GGG
Cuts at: 0 529 1293
Size: 529 764
SmlI C'TyrA_G
Cuts at: 0 928 1293
Size: 928 365
StuI AGG'CCT
Cuts at: 0 272 1183 1293
Size: 272 911 110
Fragments arranged by size:
911 272 110
StyI C'CwwG_G
Cuts at: 0 453 744 839 1293
Size: 453 291 95 454
Fragments arranged by size:
454 453 291 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 585 617 1293
Size: 430 32 123 32 676
Fragments arranged by size:
676 430 123 32 32
XmaI C'CCGG_G
Cuts at: 0 527 1293
Size: 527 766
XmnI GAAnn'nnTTC
Cuts at: 0 920 1293
Size: 920 373
ZraI GAC'GTC
Cuts at: 0 468 1293
Size: 468 825
Enzymes that do cut:
AatII AcuI AlfI AloI AlwNI ApoI AvaI BaeI
BanII BciVI BlpI BmgBI BpmI BpuEI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrFI BssSI BstYI BtgI BtsI EarI Eco57MI EcoNI
EcoO109I FalI Hin4I HincII MmeI NcoI PfoI PpuMI
PsiI PstI SanDI SapI SfcI SmaI SmlI StuI
StyI TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII Bme1580I BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI