Restriction map for
pF1KSDA0957
(Linear) (Six-base) MAPSORT of: KIAA0957.seq Check: 2536 from: 1 to: 2076
KIAA0957.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 2076
Size: 205 1871
AhdI GACnn_n'nnGTC
Cuts at: 0 1532 2076
Size: 1532 544
AleI CACnn'nnGTG
Cuts at: 0 250 2076
Size: 250 1826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 161 557 2076
Size: 161 396 1519
Fragments arranged by size:
1519 396 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 2076
Size: 442 32 1602
Fragments arranged by size:
1602 442 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1431 1626 1817 2076
Size: 1431 195 191 259
Fragments arranged by size:
1431 259 195 191
ApaI G_GGCC'C
Cuts at: 0 1730 2076
Size: 1730 346
BanI G'GyrC_C
Cuts at: 0 97 1113 2076
Size: 97 1016 963
Fragments arranged by size:
1016 963 97
BanII G_rGCy'C
Cuts at: 0 1730 2076
Size: 1730 346
BbeI G_GCGC'C
Cuts at: 0 101 2076
Size: 101 1975
BbsI GAAGACnn'nnnn_
Cuts at: 0 1625 2076
Size: 1625 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 311 2076
Size: 277 34 1765
Fragments arranged by size:
1765 277 34
BclI T'GATC_A
Cuts at: 0 1713 2076
Size: 1713 363
BglI GCCn_nnn'nGGC
Cuts at: 0 1782 2076
Size: 1782 294
BglII A'GATC_T
Cuts at: 0 176 1708 2076
Size: 176 1532 368
Fragments arranged by size:
1532 368 176
Bme1580I G_kGCm'C
Cuts at: 0 300 1730 1794 2076
Size: 300 1430 64 282
Fragments arranged by size:
1430 300 282 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 1893 2076
Size: 1893 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 751 1177 1453 1469 1547 1600 1909
Size: 638 113 426 276 16 78 53 309
Cuts at: 1909 2076
Size: 167
Fragments arranged by size:
638 426 309 276 167 113 78 53
16
BsaHI Gr'CG_yC
Cuts at: 0 98 2076
Size: 98 1978
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 442 472 768 798 1150 1180
Size: 383 30 29 30 296 30 352 30
Cuts at: 1180 1615 1645 2076
Size: 435 30 431
Fragments arranged by size:
435 431 383 352 296 30 30 30
30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 568 921 1198 2076
Size: 565 3 353 277 878
Fragments arranged by size:
878 565 353 277 3
BseYI C'CCAG_C
Cuts at: 0 817 1200 1233 1256 1723 1793 1988 2076
Size: 817 383 33 23 467 70 195 88
Fragments arranged by size:
817 467 383 195 88 70 33 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 418 2076
Size: 8 410 1658
Fragments arranged by size:
1658 410 8
BsiEI CG_ry'CG
Cuts at: 0 20 2076
Size: 20 2056
BsiHKAI G_wGCw'C
Cuts at: 0 469 2076
Size: 469 1607
Bsp1286I G_dGCh'C
Cuts at: 0 300 469 1730 1794 2076
Size: 300 169 1261 64 282
Fragments arranged by size:
1261 300 282 169 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 995 1522 2076
Size: 995 527 554
Fragments arranged by size:
995 554 527
BsrGI T'GTAC_A
Cuts at: 0 1642 2076
Size: 1642 434
BssHII G'CGCG_C
Cuts at: 0 544 2076
Size: 544 1532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1266 2076
Size: 1266 810
BstEII G'GTnAC_C
Cuts at: 0 111 2076
Size: 111 1965
BstXI CCAn_nnnn'nTGG
Cuts at: 0 217 252 970 2076
Size: 217 35 718 1106
Fragments arranged by size:
1106 718 217 35
BstYI r'GATC_y
Cuts at: 0 176 1708 2076
Size: 176 1532 368
Fragments arranged by size:
1532 368 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 255 2076
Size: 255 1821
BtsI GCAGTG_nn'
Cuts at: 0 581 801 828 1598 2076
Size: 581 220 27 770 478
Fragments arranged by size:
770 581 478 220 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 2076
Size: 205 1871
EaeI y'GGCC_r
Cuts at: 0 123 485 665 836 2076
Size: 123 362 180 171 1240
Fragments arranged by size:
1240 362 180 171 123
EarI CTCTTCn'nnn_
Cuts at: 0 773 854 1762 2027 2076
Size: 773 81 908 265 49
Fragments arranged by size:
908 773 265 81 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 638 751 1177 1453 1469 1547 1600
Size: 205 433 113 426 276 16 78 53
Cuts at: 1600 1909 2076
Size: 309 167
Fragments arranged by size:
433 426 309 276 205 167 113 78
53 16
EcoO109I rG'GnC_Cy
Cuts at: 0 491 1460 1587 1727 2076
Size: 491 969 127 140 349
Fragments arranged by size:
969 491 349 140 127
EcoRV GAT'ATC
Cuts at: 0 1382 2076
Size: 1382 694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 784 2056 2076
Size: 784 1272 20
Fragments arranged by size:
1272 784 20
HaeII r_GCGC'y
Cuts at: 0 39 101 280 2076
Size: 39 62 179 1796
Fragments arranged by size:
1796 179 62 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 1189 1221 1413 1445 1518 1550
Size: 853 32 304 32 192 32 73 32
Cuts at: 1550 1615 1647 1845 1877 2076
Size: 65 32 198 32 199
Fragments arranged by size:
853 304 199 198 192 73 65 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 708 1737 2076
Size: 708 1029 339
Fragments arranged by size:
1029 708 339
HindIII A'AGCT_T
Cuts at: 0 1441 2076
Size: 1441 635
HpaI GTT'AAC
Cuts at: 0 708 2076
Size: 708 1368
KasI G'GCGC_C
Cuts at: 0 97 2076
Size: 97 1979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1574 2076
Size: 1148 426 502
Fragments arranged by size:
1148 502 426
MscI TGG'CCA
Cuts at: 0 667 838 2076
Size: 667 171 1238
Fragments arranged by size:
1238 667 171
MslI CAynn'nnrTG
Cuts at: 0 250 2076
Size: 250 1826
MspA1I CmG'CkG
Cuts at: 0 729 2076
Size: 729 1347
NarI GG'CG_CC
Cuts at: 0 98 2076
Size: 98 1978
NspI r_CATG'y
Cuts at: 0 994 1416 2076
Size: 994 422 660
Fragments arranged by size:
994 660 422
PasI CC'CwG_GG
Cuts at: 0 789 2076
Size: 789 1287
PflMI CCAn_nnn'nTGG
Cuts at: 0 1123 1521 1887 2076
Size: 1123 398 366 189
Fragments arranged by size:
1123 398 366 189
PfoI T'CCnGG_A
Cuts at: 0 209 2076
Size: 209 1867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 2076
Size: 442 32 1602
Fragments arranged by size:
1602 442 32
PpuMI rG'GwC_Cy
Cuts at: 0 491 2076
Size: 491 1585
PspOMI G'GGCC_C
Cuts at: 0 1726 2076
Size: 1726 350
PstI C_TGCA'G
Cuts at: 0 1308 1468 1533 2076
Size: 1308 160 65 543
Fragments arranged by size:
1308 543 160 65
SanDI GG'GwC_CC
Cuts at: 0 491 2076
Size: 491 1585
SapI GCTCTTCn'nnn_
Cuts at: 0 773 2076
Size: 773 1303
SexAI A'CCwGG_T
Cuts at: 0 1405 2076
Size: 1405 671
SfcI C'TryA_G
Cuts at: 0 1304 1464 1502 1529 2076
Size: 1304 160 38 27 547
Fragments arranged by size:
1304 547 160 38 27
SfoI GGC'GCC
Cuts at: 0 99 2076
Size: 99 1977
SphI G_CATG'C
Cuts at: 0 994 2076
Size: 994 1082
StyI C'CwwG_G
Cuts at: 0 923 1385 2076
Size: 923 462 691
Fragments arranged by size:
923 691 462
TatI w'GTAC_w
Cuts at: 0 1642 2076
Size: 1642 434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 2076
Size: 383 32 1661
Fragments arranged by size:
1661 383 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 2076
Size: 675 1401
Enzymes that do cut:
AcuI AhdI AleI AlfI AloI AlwNI ApaI BanI
BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I
BmrI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrGI BssHII BstAPI BstEII BstXI
BstYI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
MmeI MscI MslI MspA1I NarI NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI SanDI SapI SexAI
SfcI SfoI SphI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BciVI BfrBI BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssSI
BstBI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI