Restriction map for
pF1KSDA0959
(Linear) (Six-base) MAPSORT of: KIAA0959.seq Check: 5002 from: 1 to: 2304
KIAA0959.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1954 2304
Size: 1954 350
AatII G_ACGT'C
Cuts at: 0 1894 2304
Size: 1894 410
AccI GT'mk_AC
Cuts at: 0 957 2304
Size: 957 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 985 1285 2071 2304
Size: 618 367 300 786 233
Fragments arranged by size:
786 618 367 300 233
AfeI AGC'GCT
Cuts at: 0 1876 2304
Size: 1876 428
AflIII A'CryG_T
Cuts at: 0 1985 2304
Size: 1985 319
AhdI GACnn_n'nnGTC
Cuts at: 0 169 2304
Size: 169 2135
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1455 1504 1696 2304
Size: 510 945 49 192 608
Fragments arranged by size:
945 608 510 192 49
ApoI r'AATT_y
Cuts at: 0 679 969 1153 1351 1797 2304
Size: 679 290 184 198 446 507
Fragments arranged by size:
679 507 446 290 198 184
BanI G'GyrC_C
Cuts at: 0 214 1261 2304
Size: 214 1047 1043
Fragments arranged by size:
1047 1043 214
BanII G_rGCy'C
Cuts at: 0 31 485 544 1639 1708 2304
Size: 31 454 59 1095 69 596
Fragments arranged by size:
1095 596 454 69 59 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 1805 1946 1976 2304
Size: 476 1329 141 30 328
Fragments arranged by size:
1329 476 328 141 30
BbvCI CC'TCA_GC
Cuts at: 0 1697 2304
Size: 1697 607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1893 1927 2304
Size: 1893 34 377
Fragments arranged by size:
1893 377 34
BclI T'GATC_A
Cuts at: 0 416 1583 2304
Size: 416 1167 721
Fragments arranged by size:
1167 721 416
BglII A'GATC_T
Cuts at: 0 699 2304
Size: 699 1605
Bme1580I G_kGCm'C
Cuts at: 0 1253 1264 2304
Size: 1253 11 1040
Fragments arranged by size:
1253 1040 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 2304
Size: 51 2253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1722 1779 2304
Size: 1722 57 525
Fragments arranged by size:
1722 525 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 679 1201 2295 2304
Size: 143 536 522 1094 9
Fragments arranged by size:
1094 536 522 143 9
Bpu10I CC'TnA_GC
Cuts at: 0 1697 2304
Size: 1697 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1002 2304
Size: 595 407 1302
Fragments arranged by size:
1302 595 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 788 2304
Size: 788 1516
BsaAI yAC'GTr
Cuts at: 0 686 2304
Size: 686 1618
BsaBI GATnn'nnATC
Cuts at: 0 940 2304
Size: 940 1364
BsaHI Gr'CG_yC
Cuts at: 0 1508 1891 2304
Size: 1508 383 413
Fragments arranged by size:
1508 413 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1626 1656 1816 1846 2304
Size: 613 30 983 30 160 30 458
Fragments arranged by size:
983 613 458 160 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1477 1480 1714 1752 1824 2095 2304
Size: 1477 3 234 38 72 271 209
Fragments arranged by size:
1477 271 234 209 72 38 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 2116 2304
Size: 485 1631 188
Fragments arranged by size:
1631 485 188
BsiHKAI G_wGCw'C
Cuts at: 0 31 564 1639 2304
Size: 31 533 1075 665
Fragments arranged by size:
1075 665 533 31
BsmI GAATG_Cn'
Cuts at: 0 429 1067 2304
Size: 429 638 1237
Fragments arranged by size:
1237 638 429
Bsp1286I G_dGCh'C
Cuts at: 0 31 485 544 564 1253 1264 1639 1708
Size: 31 454 59 20 689 11 375 69
Cuts at: 1708 2304
Size: 596
Fragments arranged by size:
689 596 454 375 69 59 31 20
11
BspHI T'CATG_A
Cuts at: 0 948 2304
Size: 948 1356
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 1954 2088 2304
Size: 248 1706 134 216
Fragments arranged by size:
1706 248 216 134
BsrGI T'GTAC_A
Cuts at: 0 1988 2304
Size: 1988 316
BssSI C'ACGA_G
Cuts at: 0 703 2304
Size: 703 1601
BstYI r'GATC_y
Cuts at: 0 699 2304
Size: 699 1605
Bsu36I CC'TnA_GG
Cuts at: 0 1803 2304
Size: 1803 501
BtgI C'CryG_G
Cuts at: 0 1718 1787 2204 2304
Size: 1718 69 417 100
Fragments arranged by size:
1718 417 100 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 2304
Size: 927 1377
BtsI GCAGTG_nn'
Cuts at: 0 762 1000 1179 1292 1622 2304
Size: 762 238 179 113 330 682
Fragments arranged by size:
762 682 330 238 179 113
EarI CTCTTCn'nnn_
Cuts at: 0 44 552 638 644 745 1531 1543 2304
Size: 44 508 86 6 101 786 12 761
Fragments arranged by size:
786 761 508 101 86 44 12 6
EcoICRI GAG'CTC
Cuts at: 0 29 1637 2304
Size: 29 1608 667
Fragments arranged by size:
1608 667 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 618 679 985 1201 1285 2071 2295
Size: 143 475 61 306 216 84 786 224
Cuts at: 2295 2304
Size: 9
Fragments arranged by size:
786 475 306 224 216 143 84 61
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 643 981 1459 2304
Size: 643 338 478 845
Fragments arranged by size:
845 643 478 338
EcoO109I rG'GnC_Cy
Cuts at: 0 446 2304
Size: 446 1858
EcoRI G'AATT_C
Cuts at: 0 679 2304
Size: 679 1625
EcoRV GAT'ATC
Cuts at: 0 1089 2304
Size: 1089 1215
FspI TGC'GCA
Cuts at: 0 2191 2304
Size: 2191 113
HaeII r_GCGC'y
Cuts at: 0 1878 2304
Size: 1878 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 905 937 1078 1110 1575 1607
Size: 155 32 718 32 141 32 465 32
Cuts at: 1607 2304
Size: 697
Fragments arranged by size:
718 697 465 155 141 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2006 2304
Size: 2006 298
HindIII A'AGCT_T
Cuts at: 0 226 2304
Size: 226 2078
MslI CAynn'nnrTG
Cuts at: 0 529 1231 1963 2304
Size: 529 702 732 341
Fragments arranged by size:
732 702 529 341
MspA1I CmG'CkG
Cuts at: 0 153 717 1501 1631 2029 2304
Size: 153 564 784 130 398 275
Fragments arranged by size:
784 564 398 275 153 130
NcoI C'CATG_G
Cuts at: 0 1718 1787 2204 2304
Size: 1718 69 417 100
Fragments arranged by size:
1718 417 100 69
NspI r_CATG'y
Cuts at: 0 1989 2304
Size: 1989 315
PciI A'CATG_T
Cuts at: 0 1985 2304
Size: 1985 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1788 2304
Size: 768 1020 516
Fragments arranged by size:
1020 768 516
PfoI T'CCnGG_A
Cuts at: 0 159 2304
Size: 159 2145
PmlI CAC'GTG
Cuts at: 0 686 2304
Size: 686 1618
PstI C_TGCA'G
Cuts at: 0 1007 1214 2304
Size: 1007 207 1090
Fragments arranged by size:
1090 1007 207
PvuII CAG'CTG
Cuts at: 0 153 717 1501 2304
Size: 153 564 784 803
Fragments arranged by size:
803 784 564 153
SacI G_AGCT'C
Cuts at: 0 31 1639 2304
Size: 31 1608 665
Fragments arranged by size:
1608 665 31
SapI GCTCTTCn'nnn_
Cuts at: 0 552 1531 2304
Size: 552 979 773
Fragments arranged by size:
979 773 552
SfcI C'TryA_G
Cuts at: 0 1003 1210 2304
Size: 1003 207 1094
Fragments arranged by size:
1094 1003 207
SmlI C'TyrA_G
Cuts at: 0 574 981 2304
Size: 574 407 1323
Fragments arranged by size:
1323 574 407
StyI C'CwwG_G
Cuts at: 0 218 1051 1718 1787 2204 2304
Size: 218 833 667 69 417 100
Fragments arranged by size:
833 667 417 218 100 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1073 1219 2304
Size: 1073 146 1085
Fragments arranged by size:
1085 1073 146
TatI w'GTAC_w
Cuts at: 0 1988 2304
Size: 1988 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1626 1658 2304
Size: 1626 32 646
Fragments arranged by size:
1626 646 32
Tth111I GACn'n_nGTC
Cuts at: 0 1657 2304
Size: 1657 647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1716 2304
Size: 1716 588
ZraI GAC'GTC
Cuts at: 0 1892 2304
Size: 1892 412
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlwNI
ApoI BanI BanII BbsI BbvCI BcgI BclI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BssSI BstYI Bsu36I BtgI BtgZI
BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII MslI MspA1I NcoI
NspI PciI PflMI PfoI PmlI PstI PvuII SacI
SapI SfcI SmlI StyI TaqII TatI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BciVI BfrBI BglI BlpI BmgBI BmtI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI FalI FseI
FspAI HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XhoI
XmaI XmnI