Restriction map for
pF1KSDA0961
(Linear) (Six-base) MAPSORT of: KIAA0961.seq Check: 1670 from: 1 to: 1557
KIAA0961.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 38 606 1557
Size: 38 568 951
Fragments arranged by size:
951 568 38
AflIII A'CryG_T
Cuts at: 0 670 1557
Size: 670 887
AhdI GACnn_n'nnGTC
Cuts at: 0 1088 1557
Size: 1088 469
AleI CACnn'nnGTG
Cuts at: 0 794 1046 1557
Size: 794 252 511
Fragments arranged by size:
794 511 252
AlwNI CAG_nnn'CTG
Cuts at: 0 870 1557
Size: 870 687
ApoI r'AATT_y
Cuts at: 0 84 533 617 701 1037 1457 1541 1557
Size: 84 449 84 84 336 420 84 16
Fragments arranged by size:
449 420 336 84 84 84 84 16
BanII G_rGCy'C
Cuts at: 0 194 1557
Size: 194 1363
BbsI GAAGACnn'nnnn_
Cuts at: 0 1087 1339 1557
Size: 1087 252 218
Fragments arranged by size:
1087 252 218
BclI T'GATC_A
Cuts at: 0 164 1557
Size: 164 1393
BglII A'GATC_T
Cuts at: 0 662 1557
Size: 662 895
Bme1580I G_kGCm'C
Cuts at: 0 597 1557
Size: 597 960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1557
Size: 983 574
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 1010 1040 1557
Size: 758 30 222 30 517
Fragments arranged by size:
758 517 222 30 30
BsmI GAATG_Cn'
Cuts at: 0 67 1557
Size: 67 1490
Bsp1286I G_dGCh'C
Cuts at: 0 194 597 1557
Size: 194 403 960
Fragments arranged by size:
960 403 194
BspHI T'CATG_A
Cuts at: 0 360 1557
Size: 360 1197
BsrDI GCAATG_nn'
Cuts at: 0 875 1557
Size: 875 682
BsrGI T'GTAC_A
Cuts at: 0 89 1557
Size: 89 1468
BssSI C'ACGA_G
Cuts at: 0 5 1557
Size: 5 1552
BstYI r'GATC_y
Cuts at: 0 662 1557
Size: 662 895
EarI CTCTTCn'nnn_
Cuts at: 0 437 1557
Size: 437 1120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1557
Size: 983 574
EcoRI G'AATT_C
Cuts at: 0 533 617 701 1037 1457 1541 1557
Size: 533 84 84 336 420 84 16
Fragments arranged by size:
533 420 336 84 84 84 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 1074 1106 1434 1466 1557
Size: 226 32 816 32 328 32 91
Fragments arranged by size:
816 328 226 91 32 32 32
HincII GTy'rAC
Cuts at: 0 607 1557
Size: 607 950
MslI CAynn'nnrTG
Cuts at: 0 542 710 794 1046 1130 1298 1382 1466
Size: 542 168 84 252 84 168 84 84
Cuts at: 1466 1482 1557
Size: 16 75
Fragments arranged by size:
542 252 168 168 84 84 84 84
75 16
NspI r_CATG'y
Cuts at: 0 674 1557
Size: 674 883
PciI A'CATG_T
Cuts at: 0 670 1557
Size: 670 887
SalI G'TCGA_C
Cuts at: 0 605 1557
Size: 605 952
SfcI C'TryA_G
Cuts at: 0 35 114 1557
Size: 35 79 1443
Fragments arranged by size:
1443 79 35
StuI AGG'CCT
Cuts at: 0 832 916 935 1000 1420 1557
Size: 832 84 19 65 420 137
Fragments arranged by size:
832 420 137 84 65 19
TatI w'GTAC_w
Cuts at: 0 89 690 928 1557
Size: 89 601 238 629
Fragments arranged by size:
629 601 238 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 417 449 1557
Size: 417 32 1108
Fragments arranged by size:
1108 417 32
XbaI T'CTAG_A
Cuts at: 0 231 1557
Size: 231 1326
XmnI GAAnn'nnTTC
Cuts at: 0 1084 1461 1557
Size: 1084 377 96
Fragments arranged by size:
1084 377 96
Enzymes that do cut:
AccI AflIII AhdI AleI AlwNI ApoI BanII BbsI
BclI BglII Bme1580I BpmI BsaXI BsmI Bsp1286I BspHI
BsrDI BsrGI BssSI BstYI EarI Eco57MI EcoRI Hin4I
HincII MslI NspI PciI SalI SfcI StuI TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII Tth111I XcmI XhoI XmaI
ZraI