Restriction map for pF1KSDA0965
 (Linear) (Six-base) MAPSORT of: KIAA0965.seq  Check: 2417  from: 1  to: 1392



KIAA0965.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     381    1392 
   Size:        381    1011
 
AccI GT'mk_AC

Cuts at:      0    1157    1392 
   Size:       1157     235
 
Acc65I G'GTAC_C

Cuts at:      0    1041    1392 
   Size:       1041     351
 
AhdI GACnn_n'nnGTC

Cuts at:      0     270     918    1392 
   Size:        270     648     474

  Fragments arranged by size: 

                 648     474     270
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     556     588    1392 
   Size:        556      32     804

  Fragments arranged by size: 

                 804     556      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     589    1392 
   Size:        589     803
 
ApoI r'AATT_y

Cuts at:      0      87     502     733    1227    1392 
   Size:         87     415     231     494     165

  Fragments arranged by size: 

                 494     415     231     165      87
 
AvaI C'yCGr_G

Cuts at:      0      50    1392 
   Size:         50    1342
 
BanI G'GyrC_C

Cuts at:      0     439    1041    1392 
   Size:        439     602     351

  Fragments arranged by size: 

                 602     439     351
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1170    1314    1392 
   Size:       1170     144      78

  Fragments arranged by size: 

                1170     144      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     857    1019    1392 
   Size:        533     324     162     373

  Fragments arranged by size: 

                 533     373     324     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     968    1392 
   Size:        968     424
 
BsaWI w'CCGG_w

Cuts at:      0    1284    1392 
   Size:       1284     108
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     308    1392 
   Size:        308    1084
 
BseYI C'CCAG_C

Cuts at:      0    1381    1392 
   Size:       1381      11
 
BsiHKAI G_wGCw'C

Cuts at:      0    1169    1392 
   Size:       1169     223
 
Bsp1286I G_dGCh'C

Cuts at:      0    1169    1392 
   Size:       1169     223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     381    1392 
   Size:        381    1011
 
BsrDI GCAATG_nn'

Cuts at:      0      11     356     529     866    1392 
   Size:         11     345     173     337     526

  Fragments arranged by size: 

                 526     345     337     173      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     439     604    1392 
   Size:        439     165     788

  Fragments arranged by size: 

                 788     439     165
 
BtgI C'CryG_G

Cuts at:      0     155    1392 
   Size:        155    1237
 
DraIII CAC_nnn'GTG

Cuts at:      0     766    1392 
   Size:        766     626
 
EaeI y'GGCC_r

Cuts at:      0     152    1392 
   Size:        152    1240
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     176     285     554    1392 
   Size:        113      63     109     269     838

  Fragments arranged by size: 

                 838     269     113     109      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     857    1019    1392 
   Size:        533     324     162     373

  Fragments arranged by size: 

                 533     373     324     162
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1329    1392 
   Size:       1329      63
 
EcoRV GAT'ATC

Cuts at:      0     958    1392 
   Size:        958     434
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     314    1392 
   Size:        314    1078
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     904     936    1279    1311    1392 
   Size:        256      32     616      32     343      32      81

  Fragments arranged by size: 

                 616     343     256      81      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1158    1346    1392 
   Size:       1158     188      46

  Fragments arranged by size: 

                1158     188      46
 
KpnI G_GTAC'C

Cuts at:      0    1045    1392 
   Size:       1045     347
 
MscI TGG'CCA

Cuts at:      0     154    1392 
   Size:        154    1238
 
MslI CAynn'nnrTG

Cuts at:      0     344    1372    1392 
   Size:        344    1028      20

  Fragments arranged by size: 

                1028     344      20
 
NcoI C'CATG_G

Cuts at:      0     155    1392 
   Size:        155    1237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     618    1304    1392 
   Size:        618     686      88

  Fragments arranged by size: 

                 686     618      88
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     775     807    1392 
   Size:        775      32     585

  Fragments arranged by size: 

                 775     585      32
 
SalI G'TCGA_C

Cuts at:      0    1156    1392 
   Size:       1156     236
 
SfcI C'TryA_G

Cuts at:      0      65    1196    1392 
   Size:         65    1131     196

  Fragments arranged by size: 

                1131     196      65
 
SmaI CCC'GGG

Cuts at:      0      52    1392 
   Size:         52    1340
 
SmlI C'TyrA_G

Cuts at:      0     983    1392 
   Size:        983     409
 
StyI C'CwwG_G

Cuts at:      0     155     668    1061    1392 
   Size:        155     513     393     331

  Fragments arranged by size: 

                 513     393     331     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     435    1392 
   Size:        435     957
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78     255    1392 
   Size:         78     177    1137

  Fragments arranged by size: 

                1137     177      78
 
XmaI C'CCGG_G

Cuts at:      0      50    1392 
   Size:         50    1342
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AhdI     AloI    AlwNI     ApoI     AvaI
     BanI     BmrI     BpmI    BpuEI    BsaWI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrDI   BstAPI     BtgI   DraIII     EaeI     EarI
  Eco57MI    EcoNI    EcoRV     FalI    Hin4I   HincII     KpnI     MscI
     MslI     NcoI    PflMI     PpiI     SalI     SfcI     SmaI     SmlI
     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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