Restriction map for
pF1KA0972
(Linear) (Six-base) MAPSORT of: KIAA0972.seq Check: 5405 from: 1 to: 2049
KIAA0972.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 932 2049
Size: 932 1117
AatII G_ACGT'C
Cuts at: 0 1165 2049
Size: 1165 884
AccI GT'mk_AC
Cuts at: 0 2002 2049
Size: 2002 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 2049
Size: 509 1540
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 2049
Size: 298 32 1719
Fragments arranged by size:
1719 298 32
ApoI r'AATT_y
Cuts at: 0 160 328 543 562 624 670 856 1355
Size: 160 168 215 19 62 46 186 499
Cuts at: 1355 1439 1523 1775 1822 1859 2024 2049
Size: 84 84 252 47 37 165 25
Fragments arranged by size:
499 252 215 186 168 165 160 84
84 62 47 46 37 25 19
AseI AT'TA_AT
Cuts at: 0 572 2049
Size: 572 1477
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 249 282 2049
Size: 249 33 1767
Fragments arranged by size:
1767 249 33
BclI T'GATC_A
Cuts at: 0 374 1848 2049
Size: 374 1474 201
Fragments arranged by size:
1474 374 201
BmgBI CAC'GTC
Cuts at: 0 150 2049
Size: 150 1899
BmrI ACTGGGnnnn_n'
Cuts at: 0 1625 1793 2049
Size: 1625 168 256
Fragments arranged by size:
1625 256 168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 2049
Size: 348 1701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 683 2049
Size: 247 436 1366
Fragments arranged by size:
1366 436 247
Bpu10I CC'TnA_GC
Cuts at: 0 2008 2049
Size: 2008 41
BsaHI Gr'CG_yC
Cuts at: 0 1162 2049
Size: 1162 887
BsaWI w'CCGG_w
Cuts at: 0 1829 2049
Size: 1829 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 2049
Size: 298 30 1721
Fragments arranged by size:
1721 298 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 319 337 2049
Size: 187 132 18 1712
Fragments arranged by size:
1712 187 132 18
BsmI GAATG_Cn'
Cuts at: 0 1975 2049
Size: 1975 74
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 2049
Size: 252 1797
BspEI T'CCGG_A
Cuts at: 0 1829 2049
Size: 1829 220
BspHI T'CATG_A
Cuts at: 0 1137 2049
Size: 1137 912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 932 2049
Size: 932 1117
BsrDI GCAATG_nn'
Cuts at: 0 1980 2049
Size: 1980 69
BsrGI T'GTAC_A
Cuts at: 0 209 2049
Size: 209 1840
Bsu36I CC'TnA_GG
Cuts at: 0 1423 2049
Size: 1423 626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1542 1577 2049
Size: 1542 35 472
Fragments arranged by size:
1542 472 35
DraI TTT'AAA
Cuts at: 0 1800 2049
Size: 1800 249
EaeI y'GGCC_r
Cuts at: 0 1575 1911 2049
Size: 1575 336 138
Fragments arranged by size:
1575 336 138
EarI CTCTTCn'nnn_
Cuts at: 0 449 1073 2049
Size: 449 624 976
Fragments arranged by size:
976 624 449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 683 2049
Size: 247 436 1366
Fragments arranged by size:
1366 436 247
EcoRI G'AATT_C
Cuts at: 0 160 328 624 1355 1439 1523 1775 1859
Size: 160 168 296 731 84 84 252 84
Cuts at: 1859 2049
Size: 190
Fragments arranged by size:
731 296 252 190 168 160 84 84
84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1858 2049
Size: 1858 191
HincII GTy'rAC
Cuts at: 0 962 1418 2049
Size: 962 456 631
Fragments arranged by size:
962 631 456
HindIII A'AGCT_T
Cuts at: 0 755 2010 2049
Size: 755 1255 39
Fragments arranged by size:
1255 755 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 2049
Size: 272 1777
MscI TGG'CCA
Cuts at: 0 1577 1913 2049
Size: 1577 336 136
Fragments arranged by size:
1577 336 136
MslI CAynn'nnrTG
Cuts at: 0 1296 1716 1800 2049
Size: 1296 420 84 249
Fragments arranged by size:
1296 420 249 84
MspA1I CmG'CkG
Cuts at: 0 1069 2049
Size: 1069 980
NspI r_CATG'y
Cuts at: 0 490 2049
Size: 490 1559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 2049
Size: 298 32 1719
Fragments arranged by size:
1719 298 32
PsiI TTA'TAA
Cuts at: 0 710 2049
Size: 710 1339
PvuII CAG'CTG
Cuts at: 0 1069 2049
Size: 1069 980
ScaI AGT'ACT
Cuts at: 0 1344 2049
Size: 1344 705
SfcI C'TryA_G
Cuts at: 0 155 234 446 2049
Size: 155 79 212 1603
Fragments arranged by size:
1603 212 155 79
StyI C'CwwG_G
Cuts at: 0 310 2049
Size: 310 1739
SwaI ATTT'AAAT
Cuts at: 0 1800 2049
Size: 1800 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 362 2049
Size: 181 181 1687
Fragments arranged by size:
1687 181 181
TatI w'GTAC_w
Cuts at: 0 209 1342 2049
Size: 209 1133 707
Fragments arranged by size:
1133 707 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 1935 1967 2049
Size: 855 32 1048 32 82
Fragments arranged by size:
1048 855 82 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 901 2049
Size: 901 1148
XmnI GAAnn'nnTTC
Cuts at: 0 585 719 1234 1711 2049
Size: 585 134 515 477 338
Fragments arranged by size:
585 515 477 338 134
ZraI GAC'GTC
Cuts at: 0 1163 2049
Size: 1163 886
Enzymes that do cut:
AarI AatII AccI AlfI AloI ApoI AseI BaeI
BclI BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI Bsu36I CspCI DraI EaeI EarI Eco57MI EcoRI
FalI HincII HindIII MmeI MscI MslI MspA1I NspI
PpiI PsiI PvuII ScaI SfcI StyI SwaI TaqII
TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmtI BpuEI BsaI
BsaAI BsaBI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI Tth111I XbaI XhoI XmaI