Restriction map for
pF1KA0973
(Linear) (Six-base) MAPSORT of: KIAA0973.seq Check: 5783 from: 1 to: 4710
KIAA0973.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 911 4710
Size: 911 3799
AatII G_ACGT'C
Cuts at: 0 4157 4710
Size: 4157 553
AccI GT'mk_AC
Cuts at: 0 542 728 1163 2957 4710
Size: 542 186 435 1794 1753
Fragments arranged by size:
1794 1753 542 435 186
Acc65I G'GTAC_C
Cuts at: 0 4299 4710
Size: 4299 411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1408 2115 2403 4667 4710
Size: 1408 707 288 2264 43
Fragments arranged by size:
2264 1408 707 288 43
AfeI AGC'GCT
Cuts at: 0 1195 1403 2914 4435 4710
Size: 1195 208 1511 1521 275
Fragments arranged by size:
1521 1511 1195 275 208
AflII C'TTAA_G
Cuts at: 0 3121 4710
Size: 3121 1589
AleI CACnn'nnGTG
Cuts at: 0 3064 4710
Size: 3064 1646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1214 3935 4710
Size: 1214 2721 775
Fragments arranged by size:
2721 1214 775
AlwNI CAG_nnn'CTG
Cuts at: 0 2746 4356 4710
Size: 2746 1610 354
Fragments arranged by size:
2746 1610 354
ApaI G_GGCC'C
Cuts at: 0 2233 2511 4710
Size: 2233 278 2199
Fragments arranged by size:
2233 2199 278
ApaLI G'TGCA_C
Cuts at: 0 1480 4104 4553 4710
Size: 1480 2624 449 157
Fragments arranged by size:
2624 1480 449 157
ApoI r'AATT_y
Cuts at: 0 877 4710
Size: 877 3833
AscI GG'CGCG_CC
Cuts at: 0 3962 4710
Size: 3962 748
AvaI C'yCGr_G
Cuts at: 0 490 683 886 1616 1666 1819 2233 2916
Size: 490 193 203 730 50 153 414 683
Cuts at: 2916 3372 3480 3859 4039 4086 4327 4411 4421
Size: 456 108 379 180 47 241 84 10
Cuts at: 4421 4602 4701 4710
Size: 181 99 9
Fragments arranged by size:
730 683 490 456 414 379 241 203
193 181 180 153 108 99 84 50
47 10 9
AvrII C'CTAG_G
Cuts at: 0 168 3447 4710
Size: 168 3279 1263
Fragments arranged by size:
3279 1263 168
BamHI G'GATC_C
Cuts at: 0 2491 4710
Size: 2491 2219
BanI G'GyrC_C
Cuts at: 0 298 1147 1175 1331 2377 2536 2663 3451
Size: 298 849 28 156 1046 159 127 788
Cuts at: 3451 4045 4093 4299 4344 4398 4606 4637 4710
Size: 594 48 206 45 54 208 31 73
Fragments arranged by size:
1046 849 788 594 298 208 206 159
156 127 73 54 48 45 31 28
BanII G_rGCy'C
Cuts at: 0 389 1568 2093 2177 2233 2308 2411 2511
Size: 389 1179 525 84 56 75 103 100
Cuts at: 2511 2891 3379 3764 4240 4520 4603 4710
Size: 380 488 385 476 280 83 107
Fragments arranged by size:
1179 525 488 476 389 385 380 280
107 103 100 84 83 75 56
BbeI G_GCGC'C
Cuts at: 0 1335 2381 3455 4097 4402 4710
Size: 1335 1046 1074 642 305 308
Fragments arranged by size:
1335 1074 1046 642 308 305
BbsI GAAGACnn'nnnn_
Cuts at: 0 955 2296 4710
Size: 955 1341 2414
Fragments arranged by size:
2414 1341 955
BbvCI CC'TCA_GC
Cuts at: 0 484 1966 2739 4349 4710
Size: 484 1482 773 1610 361
Fragments arranged by size:
1610 1482 773 484 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3294 3328 3505 3539 4710
Size: 3294 34 177 34 1171
Fragments arranged by size:
3294 1171 177 34 34
BfrBI ATG'CAT
Cuts at: 0 2617 4710
Size: 2617 2093
BglI GCCn_nnn'nGGC
Cuts at: 0 983 2469 3031 3992 4228 4323 4710
Size: 983 1486 562 961 236 95 387
Fragments arranged by size:
1486 983 961 562 387 236 95
BlpI GC'TnA_GC
Cuts at: 0 649 4234 4471 4710
Size: 649 3585 237 239
Fragments arranged by size:
3585 649 239 237
Bme1580I G_kGCm'C
Cuts at: 0 1484 1757 2233 2511 4108 4291 4412 4557
Size: 1484 273 476 278 1597 183 121 145
Cuts at: 4557 4609 4642 4658 4710
Size: 52 33 16 52
Fragments arranged by size:
1597 1484 476 278 273 183 145 121
52 52 33 16
BmgBI CAC'GTC
Cuts at: 0 540 1011 4710
Size: 540 471 3699
Fragments arranged by size:
3699 540 471
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 2179 2723 3940 4570 4710
Size: 305 1874 544 1217 630 140
Fragments arranged by size:
1874 1217 630 544 305 140
BmtI G_CTAG'C
Cuts at: 0 1450 4710
Size: 1450 3260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2723 4065 4496 4710
Size: 2723 1342 431 214
Fragments arranged by size:
2723 1342 431 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 886 928 1042 1474 2316 2371 2425
Size: 775 111 42 114 432 842 55 54
Cuts at: 2425 2526 4035 4165 4710
Size: 101 1509 130 545
Fragments arranged by size:
1509 842 775 545 432 130 114 111
101 55 54 42
Bpu10I CC'TnA_GC
Cuts at: 0 484 1055 1966 2739 4334 4349 4710
Size: 484 571 911 773 1595 15 361
Fragments arranged by size:
1595 911 773 571 484 361 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 2510 3766 4601 4710
Size: 1477 1033 1256 835 109
Fragments arranged by size:
1477 1256 1033 835 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 272 1937 4710
Size: 135 137 1665 2773
Fragments arranged by size:
2773 1665 137 135
BsaAI yAC'GTr
Cuts at: 0 2052 3066 4710
Size: 2052 1014 1644
Fragments arranged by size:
2052 1644 1014
BsaHI Gr'CG_yC
Cuts at: 0 258 773 1332 1613 2378 3452 3511 3646
Size: 258 515 559 281 765 1074 59 135
Cuts at: 3646 3940 4094 4154 4399 4710
Size: 294 154 60 245 311
Fragments arranged by size:
1074 765 559 515 311 294 281 258
245 154 135 60 59
BsaWI w'CCGG_w
Cuts at: 0 2310 3284 4710
Size: 2310 974 1426
Fragments arranged by size:
2310 1426 974
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 2041 2071 2146 2176 4710
Size: 382 30 1629 30 75 30 2534
Fragments arranged by size:
2534 1629 382 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 327 495 595 904 1792 1846 2098
Size: 228 99 168 100 309 888 54 252
Cuts at: 2098 2101 2251 2533 2841 3769 4471 4710
Size: 3 150 282 308 928 702 239
Fragments arranged by size:
928 888 702 309 308 282 252 239
228 168 150 100 99 54 3
BseYI C'CCAG_C
Cuts at: 0 456 981 2265 2410 2473 2857 3021 3046
Size: 456 525 1284 145 63 384 164 25
Cuts at: 3046 3474 3529 3807 4436 4539 4558 4659 4710
Size: 428 55 278 629 103 19 101 51
Fragments arranged by size:
1284 629 525 456 428 384 278 164
145 103 101 63 55 51 25 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3523 3700 3811 4146 4201 4264 4569 4586
Size: 3523 177 111 335 55 63 305 17
Cuts at: 4586 4710
Size: 124
Fragments arranged by size:
3523 335 305 177 124 111 63 55
17
BsiEI CG_ry'CG
Cuts at: 0 917 3596 3800 4710
Size: 917 2679 204 910
Fragments arranged by size:
2679 917 910 204
BsiHKAI G_wGCw'C
Cuts at: 0 389 418 650 1484 2224 3487 4108 4557
Size: 389 29 232 834 740 1263 621 449
Cuts at: 4557 4710
Size: 153
Fragments arranged by size:
1263 834 740 621 449 389 232 153
29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 327 1432 3931 4710
Size: 327 1105 2499 779
Fragments arranged by size:
2499 1105 779 327
Bsp1286I G_dGCh'C
Cuts at: 0 389 418 650 1484 1568 1757 2093 2177
Size: 389 29 232 834 84 189 336 84
Cuts at: 2177 2224 2233 2308 2411 2511 2891 3379 3487
Size: 47 9 75 103 100 380 488 108
Cuts at: 3487 3764 4108 4240 4291 4412 4520 4557 4603
Size: 277 344 132 51 121 108 37 46
Cuts at: 4603 4609 4642 4658 4710
Size: 6 33 16 52
Fragments arranged by size:
834 488 389 380 344 336 277 232
189 132 121 108 108 103 100 84
84 75 52 51 47 46 37 33
29 16 9 6
BspEI T'CCGG_A
Cuts at: 0 2310 3284 4710
Size: 2310 974 1426
Fragments arranged by size:
2310 1426 974
BspHI T'CATG_A
Cuts at: 0 1568 2759 4710
Size: 1568 1191 1951
Fragments arranged by size:
1951 1568 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 3317 3963 4710
Size: 911 2406 646 747
Fragments arranged by size:
2406 911 747 646
BsrBI CCG'CTC
Cuts at: 0 473 2033 2894 3326 3347 3425 3575 3777
Size: 473 1560 861 432 21 78 150 202
Cuts at: 3777 4149 4475 4710
Size: 372 326 235
Fragments arranged by size:
1560 861 473 432 372 326 235 202
150 78 21
BsrFI r'CCGG_y
Cuts at: 0 260 2248 3396 3595 4262 4710
Size: 260 1988 1148 199 667 448
Fragments arranged by size:
1988 1148 667 448 260 199
BssHII G'CGCG_C
Cuts at: 0 3400 3839 3962 4710
Size: 3400 439 123 748
Fragments arranged by size:
3400 748 439 123
BssSI C'ACGA_G
Cuts at: 0 2170 4252 4710
Size: 2170 2082 458
Fragments arranged by size:
2170 2082 458
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1313 3393 4710
Size: 1313 2080 1317
Fragments arranged by size:
2080 1317 1313
BstBI TT'CG_AA
Cuts at: 0 3161 4710
Size: 3161 1549
BstEII G'GTnAC_C
Cuts at: 0 163 2584 4710
Size: 163 2421 2126
Fragments arranged by size:
2421 2126 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1706 1887 2349 2643 3070 4218 4710
Size: 1706 181 462 294 427 1148 492
Fragments arranged by size:
1706 1148 492 462 427 294 181
BstYI r'GATC_y
Cuts at: 0 1244 2491 4710
Size: 1244 1247 2219
Fragments arranged by size:
2219 1247 1244
Bsu36I CC'TnA_GG
Cuts at: 0 3101 4710
Size: 3101 1609
BtgI C'CryG_G
Cuts at: 0 374 722 1520 2093 2486 3894 4710
Size: 374 348 798 573 393 1408 816
Fragments arranged by size:
1408 816 798 573 393 374 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1644 3547 3597 3707 4710
Size: 1644 1903 50 110 1003
Fragments arranged by size:
1903 1644 1003 110 50
BtsI GCAGTG_nn'
Cuts at: 0 566 2744 2777 2938 3149 3658 4557 4569
Size: 566 2178 33 161 211 509 899 12
Cuts at: 4569 4574 4710
Size: 5 136
Fragments arranged by size:
2178 899 566 509 211 161 136 33
12 5
DraIII CAC_nnn'GTG
Cuts at: 0 945 2052 2944 3675 4710
Size: 945 1107 892 731 1035
Fragments arranged by size:
1107 1035 945 892 731
DrdI GACnn_nn'nnGTC
Cuts at: 0 2974 4710
Size: 2974 1736
EaeI y'GGCC_r
Cuts at: 0 444 635 914 2246 3593 3707 3797 4134
Size: 444 191 279 1332 1347 114 90 337
Cuts at: 4134 4208 4220 4710
Size: 74 12 490
Fragments arranged by size:
1347 1332 490 444 337 279 191 114
90 74 12
EagI C'GGCC_G
Cuts at: 0 914 3593 3797 4710
Size: 914 2679 204 913
Fragments arranged by size:
2679 914 913 204
EarI CTCTTCn'nnn_
Cuts at: 0 2118 2692 3286 4710
Size: 2118 574 594 1424
Fragments arranged by size:
2118 1424 594 574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 265 964 2092 3231 3429 3973 4710
Size: 58 207 699 1128 1139 198 544 737
Fragments arranged by size:
1139 1128 737 699 544 207 198 58
EcoICRI GAG'CTC
Cuts at: 0 387 4710
Size: 387 4323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 886 928 1042 1408 1474 2115 2316
Size: 775 111 42 114 366 66 641 201
Cuts at: 2316 2371 2403 2425 2526 4035 4165 4667 4710
Size: 55 32 22 101 1509 130 502 43
Fragments arranged by size:
1509 775 641 502 366 201 130 114
111 101 66 55 43 42 32 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 243 907 4710
Size: 243 664 3803
Fragments arranged by size:
3803 664 243
EcoO109I rG'GnC_Cy
Cuts at: 0 1646 1833 2229 2230 2508 3051 3954 4416
Size: 1646 187 396 1 278 543 903 462
Cuts at: 4416 4710
Size: 294
Fragments arranged by size:
1646 903 543 462 396 294 278 187
1
EcoRI G'AATT_C
Cuts at: 0 877 4710
Size: 877 3833
EcoRV GAT'ATC
Cuts at: 0 1272 4710
Size: 1272 3438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1242 2949 4710
Size: 1242 1707 1761
Fragments arranged by size:
1761 1707 1242
FspI TGC'GCA
Cuts at: 0 572 2458 3847 4710
Size: 572 1886 1389 863
Fragments arranged by size:
1886 1389 863 572
HaeII r_GCGC'y
Cuts at: 0 351 498 1160 1197 1335 1405 1913 2381
Size: 351 147 662 37 138 70 508 468
Cuts at: 2381 2401 2426 2549 2916 3455 3910 4097 4234
Size: 20 25 123 367 539 455 187 137
Cuts at: 4234 4402 4437 4710
Size: 168 35 273
Fragments arranged by size:
662 539 508 468 455 367 351 273
187 168 147 138 137 123 70 37
35 25 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 519 551 1054 1086 1781 1813 1869 1901
Size: 519 32 503 32 695 32 56 32
Cuts at: 1901 2017 2049 2146 2178 4066 4098 4710
Size: 116 32 97 32 1888 32 612
Fragments arranged by size:
1888 695 612 519 503 116 97 56
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1331 2377 3451 4093 4398 4710
Size: 1331 1046 1074 642 305 312
Fragments arranged by size:
1331 1074 1046 642 312 305
KpnI G_GTAC'C
Cuts at: 0 4303 4710
Size: 4303 407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1982 2504 2855 3953 4424 4710
Size: 1070 912 522 351 1098 471 286
Fragments arranged by size:
1098 1070 912 522 471 351 286
MscI TGG'CCA
Cuts at: 0 4210 4222 4710
Size: 4210 12 488
Fragments arranged by size:
4210 488 12
MslI CAynn'nnrTG
Cuts at: 0 1348 1469 1519 3064 3068 4710
Size: 1348 121 50 1545 4 1642
Fragments arranged by size:
1642 1545 1348 121 50 4
MspA1I CmG'CkG
Cuts at: 0 156 360 819 981 2160 2194 2488 2878
Size: 156 204 459 162 1179 34 294 390
Cuts at: 2878 3618 4710
Size: 740 1092
Fragments arranged by size:
1179 1092 740 459 390 294 204 162
156 34
NaeI GCC'GGC
Cuts at: 0 2250 3398 3597 4264 4710
Size: 2250 1148 199 667 446
Fragments arranged by size:
2250 1148 667 446 199
NarI GG'CG_CC
Cuts at: 0 1332 2378 3452 4094 4399 4710
Size: 1332 1046 1074 642 305 311
Fragments arranged by size:
1332 1074 1046 642 311 305
NcoI C'CATG_G
Cuts at: 0 1520 3894 4710
Size: 1520 2374 816
Fragments arranged by size:
2374 1520 816
NgoMIV G'CCGG_C
Cuts at: 0 2248 3396 3595 4262 4710
Size: 2248 1148 199 667 448
Fragments arranged by size:
2248 1148 667 448 199
NheI G'CTAG_C
Cuts at: 0 1446 4710
Size: 1446 3264
NsiI A_TGCA'T
Cuts at: 0 2619 4710
Size: 2619 2091
NspI r_CATG'y
Cuts at: 0 1311 1428 3007 4710
Size: 1311 117 1579 1703
Fragments arranged by size:
1703 1579 1311 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 906 3675 4710
Size: 906 2769 1035
Fragments arranged by size:
2769 1035 906
PfoI T'CCnGG_A
Cuts at: 0 1625 2348 4710
Size: 1625 723 2362
Fragments arranged by size:
2362 1625 723
PmlI CAC'GTG
Cuts at: 0 2052 3066 4710
Size: 2052 1014 1644
Fragments arranged by size:
2052 1644 1014
PpuMI rG'GwC_Cy
Cuts at: 0 1646 3051 4710
Size: 1646 1405 1659
Fragments arranged by size:
1659 1646 1405
PshAI GACnn'nnGTC
Cuts at: 0 4002 4576 4710
Size: 4002 574 134
Fragments arranged by size:
4002 574 134
PspOMI G'GGCC_C
Cuts at: 0 2229 2507 4710
Size: 2229 278 2203
Fragments arranged by size:
2229 2203 278
PspXI vC'TCGA_Gb
Cuts at: 0 3480 3859 4710
Size: 3480 379 851
Fragments arranged by size:
3480 851 379
PstI C_TGCA'G
Cuts at: 0 1877 2199 2772 4710
Size: 1877 322 573 1938
Fragments arranged by size:
1938 1877 573 322
PvuII CAG'CTG
Cuts at: 0 360 819 981 2160 2194 2878 4710
Size: 360 459 162 1179 34 684 1832
Fragments arranged by size:
1832 1179 684 459 360 162 34
SacI G_AGCT'C
Cuts at: 0 389 4710
Size: 389 4321
SacII CC_GC'GG
Cuts at: 0 2489 4710
Size: 2489 2221
SanDI GG'GwC_CC
Cuts at: 0 1646 4710
Size: 1646 3064
SexAI A'CCwGG_T
Cuts at: 0 1166 4710
Size: 1166 3544
SfcI C'TryA_G
Cuts at: 0 1873 2195 2768 2979 4710
Size: 1873 322 573 211 1731
Fragments arranged by size:
1873 1731 573 322 211
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2469 4710
Size: 2469 2241
SfoI GGC'GCC
Cuts at: 0 1333 2379 3453 4095 4400 4710
Size: 1333 1046 1074 642 305 310
Fragments arranged by size:
1333 1074 1046 642 310 305
SgrAI Cr'CCGG_yG
Cuts at: 0 260 4710
Size: 260 4450
SmaI CCC'GGG
Cuts at: 0 492 685 3374 4088 4329 4423 4703 4710
Size: 492 193 2689 714 241 94 280 7
Fragments arranged by size:
2689 714 492 280 241 193 94 7
SmlI C'TyrA_G
Cuts at: 0 1492 2525 3121 3480 3781 3859 4616 4710
Size: 1492 1033 596 359 301 78 757 94
Fragments arranged by size:
1492 1033 757 596 359 301 94 78
SspI AAT'ATT
Cuts at: 0 1395 4710
Size: 1395 3315
StuI AGG'CCT
Cuts at: 0 1255 2463 2703 4667 4710
Size: 1255 1208 240 1964 43
Fragments arranged by size:
1964 1255 1208 240 43
StyI C'CwwG_G
Cuts at: 0 168 929 1520 2225 2626 3245 3447 3894
Size: 168 761 591 705 401 619 202 447
Cuts at: 3894 3957 4223 4370 4710
Size: 63 266 147 340
Fragments arranged by size:
761 705 619 591 447 401 340 266
202 168 147 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 2871 2953 3076 4428 4572 4710
Size: 144 2727 82 123 1352 144 138
Fragments arranged by size:
2727 1352 144 144 138 123 82
TatI w'GTAC_w
Cuts at: 0 1427 1655 4710
Size: 1427 228 3055
Fragments arranged by size:
3055 1427 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 2041 2073 2556 2588 3616 3648
Size: 382 32 1627 32 483 32 1028 32
Cuts at: 3648 4710
Size: 1062
Fragments arranged by size:
1627 1062 1028 483 382 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 699 4710
Size: 699 4011
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1706 2370 4617 4710
Size: 573 1133 664 2247 93
Fragments arranged by size:
2247 1133 664 573 93
XhoI C'TCGA_G
Cuts at: 0 3480 3859 4710
Size: 3480 379 851
Fragments arranged by size:
3480 851 379
XmaI C'CCGG_G
Cuts at: 0 490 683 3372 4086 4327 4421 4701 4710
Size: 490 193 2689 714 241 94 280 9
Fragments arranged by size:
2689 714 490 280 241 193 94 9
XmnI GAAnn'nnTTC
Cuts at: 0 1117 4710
Size: 1117 3593
ZraI GAC'GTC
Cuts at: 0 4155 4710
Size: 4155 555
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflIII AgeI AhdI AloI AseI AsiSI BaeI
BciVI BclI BglII BsaBI BsiWI BsmI BsrDI BsrGI
BstZ17I ClaI CspCI DraI FseI FspAI HincII HindIII
HpaI MfeI MluI NdeI NotI NruI PacI PasI
PciI PmeI PpiI PsiI PsrI PvuI RsrII SalI
SapI SbfI ScaI SgfI SnaBI SpeI SphI SrfI
SwaI XbaI