Restriction map for
pF1KSDA0975
(Linear) (Six-base) MAPSORT of: KIAA0975.seq Check: 4669 from: 1 to: 4512
KIAA0975.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1780 3747 4398 4512
Size: 1780 1967 651 114
Fragments arranged by size:
1967 1780 651 114
AccI GT'mk_AC
Cuts at: 0 281 2573 2582 2990 4512
Size: 281 2292 9 408 1522
Fragments arranged by size:
2292 1522 408 281 9
Acc65I G'GTAC_C
Cuts at: 0 2930 4211 4512
Size: 2930 1281 301
Fragments arranged by size:
2930 1281 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 800 830 3043 3230 4512
Size: 410 390 30 2213 187 1282
Fragments arranged by size:
2213 1282 410 390 187 30
AflII C'TTAA_G
Cuts at: 0 559 568 4512
Size: 559 9 3944
Fragments arranged by size:
3944 559 9
AflIII A'CryG_T
Cuts at: 0 1217 4512
Size: 1217 3295
AhdI GACnn_n'nnGTC
Cuts at: 0 3696 4512
Size: 3696 816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 1871 3392 4512
Size: 479 1392 1521 1120
Fragments arranged by size:
1521 1392 1120 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3970 4002 4512
Size: 3970 32 510
Fragments arranged by size:
3970 510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 420 624 990 1687 1815 3021 3187 4512
Size: 420 204 366 697 128 1206 166 1325
Fragments arranged by size:
1325 1206 697 420 366 204 166 128
ApaI G_GGCC'C
Cuts at: 0 26 1976 2079 4099 4512
Size: 26 1950 103 2020 413
Fragments arranged by size:
2020 1950 413 103 26
ApoI r'AATT_y
Cuts at: 0 790 4512
Size: 790 3722
AvaI C'yCGr_G
Cuts at: 0 25 529 1591 2237 2885 3049 3318 4177
Size: 25 504 1062 646 648 164 269 859
Cuts at: 4177 4512
Size: 335
Fragments arranged by size:
1062 859 648 646 504 335 269 164
25
BanI G'GyrC_C
Cuts at: 0 1734 2791 2890 2930 3476 4211 4359 4512
Size: 1734 1057 99 40 546 735 148 153
Fragments arranged by size:
1734 1057 735 546 153 148 99 40
BanII G_rGCy'C
Cuts at: 0 26 70 398 1976 2079 3076 3434 4099
Size: 26 44 328 1578 103 997 358 665
Cuts at: 4099 4505 4512
Size: 406 7
Fragments arranged by size:
1578 997 665 406 358 328 103 44
26 7
BbeI G_GCGC'C
Cuts at: 0 2894 4512
Size: 2894 1618
BbsI GAAGACnn'nnnn_
Cuts at: 0 1430 2205 3031 3223 4290 4512
Size: 1430 775 826 192 1067 222
Fragments arranged by size:
1430 1067 826 775 222 192
BbvCI CC'TCA_GC
Cuts at: 0 3198 3452 3472 4033 4512
Size: 3198 254 20 561 479
Fragments arranged by size:
3198 561 479 254 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 455 3289 3323 4512
Size: 421 34 2834 34 1189
Fragments arranged by size:
2834 1189 421 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2173 4512
Size: 2173 2339
BglI GCCn_nnn'nGGC
Cuts at: 0 43 3413 4512
Size: 43 3370 1099
Fragments arranged by size:
3370 1099 43
BglII A'GATC_T
Cuts at: 0 4136 4512
Size: 4136 376
BlpI GC'TnA_GC
Cuts at: 0 686 2827 4010 4512
Size: 686 2141 1183 502
Fragments arranged by size:
2141 1183 686 502
Bme1580I G_kGCm'C
Cuts at: 0 26 536 1976 2079 4099 4512
Size: 26 510 1440 103 2020 413
Fragments arranged by size:
2020 1440 510 413 103 26
BmgBI CAC'GTC
Cuts at: 0 1677 1944 1983 3399 4308 4512
Size: 1677 267 39 1416 909 204
Fragments arranged by size:
1677 1416 909 267 204 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 3157 3306 3660 3953 4415 4512
Size: 3157 149 354 293 462 97
Fragments arranged by size:
3157 462 354 293 149 97
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 1311 4459 4512
Size: 433 878 3148 53
Fragments arranged by size:
3148 878 433 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 295 1231 2125 3164 3266 3541 3790
Size: 271 24 936 894 1039 102 275 249
Cuts at: 3790 4512
Size: 722
Fragments arranged by size:
1039 936 894 722 275 271 249 102
24
Bpu10I CC'TnA_GC
Cuts at: 0 723 880 1816 3198 3452 3472 4033 4512
Size: 723 157 936 1382 254 20 561 479
Fragments arranged by size:
1382 936 723 561 479 254 157 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 2107 2329 4512
Size: 225 1882 222 2183
Fragments arranged by size:
2183 1882 225 222
BsaAI yAC'GTr
Cuts at: 0 500 1218 2910 3767 4050 4512
Size: 500 718 1692 857 283 462
Fragments arranged by size:
1692 857 718 500 462 283
BsaHI Gr'CG_yC
Cuts at: 0 2387 2891 3268 4512
Size: 2387 504 377 1244
Fragments arranged by size:
2387 1244 504 377
BsaWI w'CCGG_w
Cuts at: 0 1184 1259 4512
Size: 1184 75 3253
Fragments arranged by size:
3253 1184 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 880 910 1015 1045 1204 1234 1372 1402
Size: 880 30 105 30 159 30 138 30
Cuts at: 1402 1467 1497 2245 2275 3862 3892 4462 4492
Size: 65 30 748 30 1587 30 570 30
Cuts at: 4492 4512
Size: 20
Fragments arranged by size:
1587 880 748 570 159 138 105 65
30 30 30 30 30 30 30 30
20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1023 1192 1561 1900 1924 1927 1936 1939
Size: 1023 169 369 339 24 3 9 3
Cuts at: 1939 1951 2002 2005 2008 2015 2035 2038 2047
Size: 12 51 3 3 7 20 3 9
Cuts at: 2047 2065 2068 2253 3277 3364 3481 3979 3982
Size: 18 3 185 1024 87 117 498 3
Cuts at: 3982 4512
Size: 530
Fragments arranged by size:
1024 1023 530 498 369 339 185 169
117 87 51 24 20 18 12 9
9 7 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 1059 1459 1505 2438 2741 2879 3138 3174
Size: 1059 400 46 933 303 138 259 36
Cuts at: 3174 3210 3228 3294 4026 4512
Size: 36 18 66 732 486
Fragments arranged by size:
1059 933 732 486 400 303 259 138
66 46 36 36 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 2173 2383 2623 2634 4072 4512
Size: 1102 1071 210 240 11 1438 440
Fragments arranged by size:
1438 1102 1071 440 240 210 11
BsiEI CG_ry'CG
Cuts at: 0 8 2612 4512
Size: 8 2604 1900
Fragments arranged by size:
2604 1900 8
BsiHKAI G_wGCw'C
Cuts at: 0 398 1217 1598 1822 2111 2192 2345 3329
Size: 398 819 381 224 289 81 153 984
Cuts at: 3329 3434 3572 4259 4505 4512
Size: 105 138 687 246 7
Fragments arranged by size:
984 819 687 398 381 289 246 224
153 138 105 81 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3259 4512
Size: 3259 1253
Bsp1286I G_dGCh'C
Cuts at: 0 26 70 398 536 1217 1598 1822 1976
Size: 26 44 328 138 681 381 224 154
Cuts at: 1976 2079 2111 2192 2345 3076 3329 3434 3572
Size: 103 32 81 153 731 253 105 138
Cuts at: 3572 4099 4259 4505 4512
Size: 527 160 246 7
Fragments arranged by size:
731 681 527 381 328 253 246 224
160 154 153 138 138 105 103 81
44 32 26 7
BspEI T'CCGG_A
Cuts at: 0 1184 4512
Size: 1184 3328
BspHI T'CATG_A
Cuts at: 0 4317 4512
Size: 4317 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 215 1780 2405 2426 3701 3747 4398
Size: 173 42 1565 625 21 1275 46 651
Cuts at: 4398 4512
Size: 114
Fragments arranged by size:
1565 1275 651 625 173 114 46 42
21
BsrBI CCG'CTC
Cuts at: 0 30 1845 2841 4512
Size: 30 1815 996 1671
Fragments arranged by size:
1815 1671 996 30
BsrDI GCAATG_nn'
Cuts at: 0 1604 3120 3446 4512
Size: 1604 1516 326 1066
Fragments arranged by size:
1604 1516 1066 326
BsrFI r'CCGG_y
Cuts at: 0 42 427 4214 4507 4512
Size: 42 385 3787 293 5
Fragments arranged by size:
3787 385 293 42 5
BssHII G'CGCG_C
Cuts at: 0 10 55 2684 4512
Size: 10 45 2629 1828
Fragments arranged by size:
2629 1828 45 10
BssSI C'ACGA_G
Cuts at: 0 2401 2923 2965 4512
Size: 2401 522 42 1547
Fragments arranged by size:
2401 1547 522 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 4512
Size: 624 3888
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 1272 4512
Size: 330 942 3240
Fragments arranged by size:
3240 942 330
BstYI r'GATC_y
Cuts at: 0 270 1146 2219 3359 4136 4512
Size: 270 876 1073 1140 777 376
Fragments arranged by size:
1140 1073 876 777 376 270
Bsu36I CC'TnA_GG
Cuts at: 0 3204 4512
Size: 3204 1308
BtgI C'CryG_G
Cuts at: 0 377 2787 4512
Size: 377 2410 1725
Fragments arranged by size:
2410 1725 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2190 3021 4512
Size: 2190 831 1491
Fragments arranged by size:
2190 1491 831
BtsI GCAGTG_nn'
Cuts at: 0 2942 3604 3795 4171 4338 4512
Size: 2942 662 191 376 167 174
Fragments arranged by size:
2942 662 376 191 174 167
EaeI y'GGCC_r
Cuts at: 0 44 1556 1746 1787 2447 2609 2904 3087
Size: 44 1512 190 41 660 162 295 183
Cuts at: 3087 4227 4482 4512
Size: 1140 255 30
Fragments arranged by size:
1512 1140 660 295 255 190 183 162
44 41 30
EagI C'GGCC_G
Cuts at: 0 2609 4512
Size: 2609 1903
EarI CTCTTCn'nnn_
Cuts at: 0 253 386 1928 2012 2024 2036 2377 3439
Size: 253 133 1542 84 12 12 341 1062
Cuts at: 3439 4512
Size: 1073
Fragments arranged by size:
1542 1073 1062 341 253 133 84 12
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 2155 2697 3574 4512
Size: 203 1952 542 877 938
Fragments arranged by size:
1952 938 877 542 203
EcoICRI GAG'CTC
Cuts at: 0 396 3432 4503 4512
Size: 396 3036 1071 9
Fragments arranged by size:
3036 1071 396 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 295 410 800 830 1231 2125 3043
Size: 271 24 115 390 30 401 894 918
Cuts at: 3043 3164 3230 3266 3541 3790 4512
Size: 121 66 36 275 249 722
Fragments arranged by size:
918 894 722 401 390 275 271 249
121 115 66 36 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 3334 4150 4512
Size: 658 2676 816 362
Fragments arranged by size:
2676 816 658 362
EcoO109I rG'GnC_Cy
Cuts at: 0 589 829 1292 1551 1972 1973 2076 3018
Size: 589 240 463 259 421 1 103 942
Cuts at: 3018 3135 3303 3978 4023 4095 4096 4242 4284
Size: 117 168 675 45 72 1 146 42
Cuts at: 4284 4473 4512
Size: 189 39
Fragments arranged by size:
942 675 589 463 421 259 240 189
168 146 117 103 72 45 42 39
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 738 2784 2994 4512
Size: 738 2046 210 1518
Fragments arranged by size:
2046 1518 738 210
HaeII r_GCGC'y
Cuts at: 0 1815 2061 2676 2894 3068 3422 4512
Size: 1815 246 615 218 174 354 1090
Fragments arranged by size:
1815 1090 615 354 246 218 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 1204 1236 1372 1404 2407 2439
Size: 689 32 483 32 136 32 1003 32
Cuts at: 2439 3682 3714 3862 3894 4512
Size: 1243 32 148 32 618
Fragments arranged by size:
1243 1003 689 618 483 148 136 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1140 3864 4512
Size: 1140 2724 648
Fragments arranged by size:
2724 1140 648
HindIII A'AGCT_T
Cuts at: 0 246 3682 4512
Size: 246 3436 830
Fragments arranged by size:
3436 830 246
KasI G'GCGC_C
Cuts at: 0 2890 4512
Size: 2890 1622
KpnI G_GTAC'C
Cuts at: 0 2934 4215 4512
Size: 2934 1281 297
Fragments arranged by size:
2934 1281 297
MscI TGG'CCA
Cuts at: 0 1558 1748 2449 2906 3089 4512
Size: 1558 190 701 457 183 1423
Fragments arranged by size:
1558 1423 701 457 190 183
MslI CAynn'nnrTG
Cuts at: 0 356 680 2449 2953 3751 3820 4512
Size: 356 324 1769 504 798 69 692
Fragments arranged by size:
1769 798 692 504 356 324 69
MspA1I CmG'CkG
Cuts at: 0 379 459 480 1441 1851 1881 2511 2648
Size: 379 80 21 961 410 30 630 137
Cuts at: 2648 2873 3000 4512
Size: 225 127 1512
Fragments arranged by size:
1512 961 630 410 379 225 137 127
80 30 21
NaeI GCC'GGC
Cuts at: 0 44 429 4512
Size: 44 385 4083
Fragments arranged by size:
4083 385 44
NarI GG'CG_CC
Cuts at: 0 2891 4512
Size: 2891 1621
NgoMIV G'CCGG_C
Cuts at: 0 42 427 4512
Size: 42 385 4085
Fragments arranged by size:
4085 385 42
NspI r_CATG'y
Cuts at: 0 1714 4074 4512
Size: 1714 2360 438
Fragments arranged by size:
2360 1714 438
PasI CC'CwG_GG
Cuts at: 0 1611 2079 3069 3514 4081 4512
Size: 1611 468 990 445 567 431
Fragments arranged by size:
1611 990 567 468 445 431
PflMI CCAn_nnn'nTGG
Cuts at: 0 2445 4005 4081 4512
Size: 2445 1560 76 431
Fragments arranged by size:
2445 1560 431 76
PfoI T'CCnGG_A
Cuts at: 0 539 941 1151 4145 4238 4512
Size: 539 402 210 2994 93 274
Fragments arranged by size:
2994 539 402 274 210 93
PmlI CAC'GTG
Cuts at: 0 500 1218 2910 3767 4512
Size: 500 718 1692 857 745
Fragments arranged by size:
1692 857 745 718 500
PpuMI rG'GwC_Cy
Cuts at: 0 589 3018 3135 3978 4242 4512
Size: 589 2429 117 843 264 270
Fragments arranged by size:
2429 843 589 270 264 117
PshAI GACnn'nnGTC
Cuts at: 0 841 950 3031 3122 4512
Size: 841 109 2081 91 1390
Fragments arranged by size:
2081 1390 841 109 91
PspOMI G'GGCC_C
Cuts at: 0 22 1972 2075 4095 4512
Size: 22 1950 103 2020 417
Fragments arranged by size:
2020 1950 417 103 22
PstI C_TGCA'G
Cuts at: 0 458 485 986 2495 3017 3192 3611 3698
Size: 458 27 501 1509 522 175 419 87
Cuts at: 3698 4093 4512
Size: 395 419
Fragments arranged by size:
1509 522 501 458 419 419 395 175
87 27
PvuII CAG'CTG
Cuts at: 0 459 480 1441 1851 2511 2648 2873 3000
Size: 459 21 961 410 660 137 225 127
Cuts at: 3000 4512
Size: 1512
Fragments arranged by size:
1512 961 660 459 410 225 137 127
21
SacI G_AGCT'C
Cuts at: 0 398 3434 4505 4512
Size: 398 3036 1071 7
Fragments arranged by size:
3036 1071 398 7
SacII CC_GC'GG
Cuts at: 0 380 4512
Size: 380 4132
SapI GCTCTTCn'nnn_
Cuts at: 0 386 2377 3439 4512
Size: 386 1991 1062 1073
Fragments arranged by size:
1991 1073 1062 386
SbfI CC_TGCA'GG
Cuts at: 0 2495 4512
Size: 2495 2017
ScaI AGT'ACT
Cuts at: 0 329 4512
Size: 329 4183
SexAI A'CCwGG_T
Cuts at: 0 2576 4512
Size: 2576 1936
SfcI C'TryA_G
Cuts at: 0 147 454 481 982 2491 2583 3013 3188
Size: 147 307 27 501 1509 92 430 175
Cuts at: 3188 3607 3694 4089 4512
Size: 419 87 395 423
Fragments arranged by size:
1509 501 430 423 419 395 307 175
147 92 87 27
SfoI GGC'GCC
Cuts at: 0 2892 4512
Size: 2892 1620
SmaI CCC'GGG
Cuts at: 0 3051 4512
Size: 3051 1461
SmlI C'TyrA_G
Cuts at: 0 240 559 568 2122 2344 4512
Size: 240 319 9 1554 222 2168
Fragments arranged by size:
2168 1554 319 240 222 9
SphI G_CATG'C
Cuts at: 0 4074 4512
Size: 4074 438
SspI AAT'ATT
Cuts at: 0 644 4512
Size: 644 3868
StuI AGG'CCT
Cuts at: 0 3238 4057 4512
Size: 3238 819 455
Fragments arranged by size:
3238 819 455
StyI C'CwwG_G
Cuts at: 0 47 1037 1265 1376 1524 1628 3551 4512
Size: 47 990 228 111 148 104 1923 961
Fragments arranged by size:
1923 990 961 228 148 111 104 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 538 2809 4073 4265 4512
Size: 538 2271 1264 192 247
Fragments arranged by size:
2271 1264 538 247 192
TatI w'GTAC_w
Cuts at: 0 327 3560 4512
Size: 327 3233 952
Fragments arranged by size:
3233 952 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1467 1499 2245 2277 4462 4494 4512
Size: 1467 32 746 32 2185 32 18
Fragments arranged by size:
2185 1467 746 32 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 4159 4512
Size: 4159 353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 2853 3906 4512
Size: 112 2741 1053 606
Fragments arranged by size:
2741 1053 606 112
XmaI C'CCGG_G
Cuts at: 0 3049 4512
Size: 3049 1463
XmnI GAAnn'nnTTC
Cuts at: 0 1046 4512
Size: 1046 3466
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI KpnI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SapI SbfI ScaI SexAI SfcI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AleI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BclI BfrBI BmtI BsaI
BsaBI BsiWI BsmI BsrGI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI HpaI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SfiI SgfI
SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI ZraI