Restriction map for
pF1KA0987
(Linear) (Six-base) MAPSORT of: KIAA0987.seq Check: 1559 from: 1 to: 3261
KIAA0987.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1655 2537 3261
Size: 1655 882 724
Fragments arranged by size:
1655 882 724
AclI AA'CG_TT
Cuts at: 0 1308 3261
Size: 1308 1953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 2363 2379 3261
Size: 611 1752 16 882
Fragments arranged by size:
1752 882 611 16
AflIII A'CryG_T
Cuts at: 0 3197 3261
Size: 3197 64
AhdI GACnn_n'nnGTC
Cuts at: 0 639 3261
Size: 639 2622
AleI CACnn'nnGTG
Cuts at: 0 169 1399 2597 3261
Size: 169 1230 1198 664
Fragments arranged by size:
1230 1198 664 169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 2330 3261
Size: 187 2143 931
Fragments arranged by size:
2143 931 187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2321 2353 3261
Size: 2321 32 908
Fragments arranged by size:
2321 908 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 577 2245 2761 3156 3261
Size: 132 445 1668 516 395 105
Fragments arranged by size:
1668 516 445 395 132 105
ApaLI G'TGCA_C
Cuts at: 0 2521 2590 3014 3261
Size: 2521 69 424 247
Fragments arranged by size:
2521 424 247 69
ApoI r'AATT_y
Cuts at: 0 559 1192 2428 2906 3261
Size: 559 633 1236 478 355
Fragments arranged by size:
1236 633 559 478 355
AvaI C'yCGr_G
Cuts at: 0 2043 3261
Size: 2043 1218
AvrII C'CTAG_G
Cuts at: 0 1695 3261
Size: 1695 1566
BanI G'GyrC_C
Cuts at: 0 90 3261
Size: 90 3171
BanII G_rGCy'C
Cuts at: 0 56 688 710 1583 1661 2006 2147 2683
Size: 56 632 22 873 78 345 141 536
Cuts at: 2683 3261
Size: 578
Fragments arranged by size:
873 632 578 536 345 141 78 56
22
BbeI G_GCGC'C
Cuts at: 0 94 3261
Size: 94 3167
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 797 2351 2860 3261
Size: 254 543 1554 509 401
Fragments arranged by size:
1554 543 509 401 254
BbvCI CC'TCA_GC
Cuts at: 0 1421 3261
Size: 1421 1840
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2904 2938 3261
Size: 2904 34 323
Fragments arranged by size:
2904 323 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1804 3261
Size: 1804 1457
BclI T'GATC_A
Cuts at: 0 1391 3261
Size: 1391 1870
BfrBI ATG'CAT
Cuts at: 0 852 3261
Size: 852 2409
BglII A'GATC_T
Cuts at: 0 2988 3261
Size: 2988 273
BlpI GC'TnA_GC
Cuts at: 0 1640 2536 3261
Size: 1640 896 725
Fragments arranged by size:
1640 896 725
Bme1580I G_kGCm'C
Cuts at: 0 1563 1901 2525 2594 3018 3261
Size: 1563 338 624 69 424 243
Fragments arranged by size:
1563 624 424 338 243 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 402 3261
Size: 402 2859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 372 1673 2020 3013 3261
Size: 154 218 1301 347 993 248
Fragments arranged by size:
1301 993 347 248 218 154
Bpu10I CC'TnA_GC
Cuts at: 0 503 908 1421 3161 3261
Size: 503 405 513 1740 100
Fragments arranged by size:
1740 513 503 405 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2040 2131 2156 3261
Size: 2040 91 25 1105
Fragments arranged by size:
2040 1105 91 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 2098 2554 3261
Size: 299 1799 456 707
Fragments arranged by size:
1799 707 456 299
BsaAI yAC'GTr
Cuts at: 0 461 3261
Size: 461 2800
BsaBI GATnn'nnATC
Cuts at: 0 899 3261
Size: 899 2362
BsaHI Gr'CG_yC
Cuts at: 0 91 832 3261
Size: 91 741 2429
Fragments arranged by size:
2429 741 91
BsaWI w'CCGG_w
Cuts at: 0 643 1407 3261
Size: 643 764 1854
Fragments arranged by size:
1854 764 643
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1389 1419 2551 2581 3261
Size: 1389 30 1132 30 680
Fragments arranged by size:
1389 1132 680 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1438 1441 2590 2734 2797 2906 3261
Size: 130 1308 3 1149 144 63 109 355
Fragments arranged by size:
1308 1149 355 144 130 109 63 3
BseYI C'CCAG_C
Cuts at: 0 681 1275 2462 3261
Size: 681 594 1187 799
Fragments arranged by size:
1187 799 681 594
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 2575 2813 3261
Size: 793 1782 238 448
Fragments arranged by size:
1782 793 448 238
BsiEI CG_ry'CG
Cuts at: 0 8 975 1057 3261
Size: 8 967 82 2204
Fragments arranged by size:
2204 967 82 8
BsiHKAI G_wGCw'C
Cuts at: 0 710 1583 1646 2147 2525 2594 3018 3261
Size: 710 873 63 501 378 69 424 243
Fragments arranged by size:
873 710 501 424 378 243 69 63
BsmI GAATG_Cn'
Cuts at: 0 2653 3261
Size: 2653 608
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2500 3261
Size: 2500 761
Bsp1286I G_dGCh'C
Cuts at: 0 56 688 710 1563 1583 1646 1661 1901
Size: 56 632 22 853 20 63 15 240
Cuts at: 1901 2006 2147 2525 2594 2683 3018 3261
Size: 105 141 378 69 89 335 243
Fragments arranged by size:
853 632 378 335 243 240 141 105
89 69 63 56 22 20 15
BspEI T'CCGG_A
Cuts at: 0 643 1407 3261
Size: 643 764 1854
Fragments arranged by size:
1854 764 643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1655 2537 3261
Size: 1655 882 724
Fragments arranged by size:
1655 882 724
BsrBI CCG'CTC
Cuts at: 0 1431 1883 2580 3261
Size: 1431 452 697 681
Fragments arranged by size:
1431 697 681 452
BsrFI r'CCGG_y
Cuts at: 0 974 3261
Size: 974 2287
BssHII G'CGCG_C
Cuts at: 0 81 3261
Size: 81 3180
BssSI C'ACGA_G
Cuts at: 0 1495 3261
Size: 1495 1766
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 2529 3261
Size: 332 2197 732
Fragments arranged by size:
2197 732 332
BstBI TT'CG_AA
Cuts at: 0 525 3261
Size: 525 2736
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3216 3261
Size: 3216 45
BstYI r'GATC_y
Cuts at: 0 1049 2988 3261
Size: 1049 1939 273
Fragments arranged by size:
1939 1049 273
BtsI GCAGTG_nn'
Cuts at: 0 337 2243 2883 3261
Size: 337 1906 640 378
Fragments arranged by size:
1906 640 378 337
DrdI GACnn_nn'nnGTC
Cuts at: 0 2355 3261
Size: 2355 906
EaeI y'GGCC_r
Cuts at: 0 1054 1350 1484 2297 3261
Size: 1054 296 134 813 964
Fragments arranged by size:
1054 964 813 296 134
EagI C'GGCC_G
Cuts at: 0 1054 3261
Size: 1054 2207
EarI CTCTTCn'nnn_
Cuts at: 0 1245 1436 1915 2057 2263 2339 2744 3261
Size: 1245 191 479 142 206 76 405 517
Fragments arranged by size:
1245 517 479 405 206 191 142 76
EcoICRI GAG'CTC
Cuts at: 0 708 1581 2145 3261
Size: 708 873 564 1116
Fragments arranged by size:
1116 873 708 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 372 611 1673 2020 2363 2379 3013
Size: 154 218 239 1062 347 343 16 634
Cuts at: 3013 3261
Size: 248
Fragments arranged by size:
1062 634 347 343 248 239 218 154
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1694 2743 3261
Size: 1694 1049 518
Fragments arranged by size:
1694 1049 518
EcoO109I rG'GnC_Cy
Cuts at: 0 129 3261
Size: 129 3132
EcoRI G'AATT_C
Cuts at: 0 2428 3261
Size: 2428 833
EcoRV GAT'ATC
Cuts at: 0 597 3261
Size: 597 2664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 361 551 3261
Size: 361 190 2710
Fragments arranged by size:
2710 361 190
HaeII r_GCGC'y
Cuts at: 0 94 2027 3157 3261
Size: 94 1933 1130 104
Fragments arranged by size:
1933 1130 104 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 620 625 652 657 1489 1521
Size: 13 32 575 5 27 5 832 32
Cuts at: 1521 2175 2207 3261
Size: 654 32 1054
Fragments arranged by size:
1054 832 654 575 32 32 32 27
13 5 5
KasI G'GCGC_C
Cuts at: 0 90 3261
Size: 90 3171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 2102 3261
Size: 473 1629 1159
Fragments arranged by size:
1629 1159 473
MscI TGG'CCA
Cuts at: 0 1352 3261
Size: 1352 1909
MslI CAynn'nnrTG
Cuts at: 0 169 1399 2597 3202 3261
Size: 169 1230 1198 605 59
Fragments arranged by size:
1230 1198 605 169 59
MspA1I CmG'CkG
Cuts at: 0 151 2630 2761 3261
Size: 151 2479 131 500
Fragments arranged by size:
2479 500 151 131
NarI GG'CG_CC
Cuts at: 0 91 3261
Size: 91 3170
NdeI CA'TA_TG
Cuts at: 0 2958 3261
Size: 2958 303
NruI TCG'CGA
Cuts at: 0 971 3261
Size: 971 2290
NsiI A_TGCA'T
Cuts at: 0 854 3261
Size: 854 2407
NspI r_CATG'y
Cuts at: 0 330 3201 3261
Size: 330 2871 60
Fragments arranged by size:
2871 330 60
PasI CC'CwG_GG
Cuts at: 0 1681 3261
Size: 1681 1580
PciI A'CATG_T
Cuts at: 0 3197 3261
Size: 3197 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 3156 3250 3261
Size: 3156 94 11
Fragments arranged by size:
3156 94 11
PfoI T'CCnGG_A
Cuts at: 0 2738 3261
Size: 2738 523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2037 2069 3261
Size: 2037 32 1192
Fragments arranged by size:
2037 1192 32
PshAI GACnn'nnGTC
Cuts at: 0 1529 3261
Size: 1529 1732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 3261
Size: 1024 32 2205
Fragments arranged by size:
2205 1024 32
PstI C_TGCA'G
Cuts at: 0 156 2643 3261
Size: 156 2487 618
Fragments arranged by size:
2487 618 156
SacI G_AGCT'C
Cuts at: 0 710 1583 2147 3261
Size: 710 873 564 1114
Fragments arranged by size:
1114 873 710 564
SapI GCTCTTCn'nnn_
Cuts at: 0 1245 3261
Size: 1245 2016
SexAI A'CCwGG_T
Cuts at: 0 601 3261
Size: 601 2660
SfcI C'TryA_G
Cuts at: 0 152 1722 2639 3261
Size: 152 1570 917 622
Fragments arranged by size:
1570 917 622 152
SfoI GGC'GCC
Cuts at: 0 92 3261
Size: 92 3169
SmlI C'TyrA_G
Cuts at: 0 2019 2146 2171 3261
Size: 2019 127 25 1090
Fragments arranged by size:
2019 1090 127 25
SnaBI TAC'GTA
Cuts at: 0 461 3261
Size: 461 2800
SphI G_CATG'C
Cuts at: 0 330 3261
Size: 330 2931
StuI AGG'CCT
Cuts at: 0 1689 3261
Size: 1689 1572
StyI C'CwwG_G
Cuts at: 0 110 1004 1202 1664 1695 2006 3261
Size: 110 894 198 462 31 311 1255
Fragments arranged by size:
1255 894 462 311 198 110 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 281 1567 3261
Size: 22 259 1286 1694
Fragments arranged by size:
1694 1286 259 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 1389 1421 3261
Size: 1122 32 235 32 1840
Fragments arranged by size:
1840 1122 235 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2490 3261
Size: 2490 771
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtsI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NdeI NruI NsiI NspI PasI PciI PflMI
PfoI PpiI PshAI PsrI PstI SacI SapI SexAI
SfcI SfoI SmlI SnaBI SphI StuI StyI TaqII
TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI ApaI AscI
AseI AsiSI BaeI BamHI BglI BmgBI BmrI BmtI
BsiWI BspHI BsrDI BsrGI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EciI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NgoMIV NheI NotI PacI PmeI PmlI PpuMI
PsiI PspOMI PspXI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SpeI
SrfI SspI SwaI TatI XbaI XcmI XhoI XmaI
XmnI ZraI