Restriction map for
pF1KSDA0988
(Linear) (Six-base) MAPSORT of: KIAA0988.seq Check: 3626 from: 1 to: 3576
KIAA0988.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AatII G_ACGT'C
Cuts at: 0 1061 3425 3576
Size: 1061 2364 151
Fragments arranged by size:
2364 1061 151
AccI GT'mk_AC
Cuts at: 0 2986 3576
Size: 2986 590
Acc65I G'GTAC_C
Cuts at: 0 950 3576
Size: 950 2626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1126 1203 1354 1468 1984 3048 3151
Size: 946 180 77 151 114 516 1064 103
Cuts at: 3151 3576
Size: 425
Fragments arranged by size:
1064 946 516 425 180 151 114 103
77
AflIII A'CryG_T
Cuts at: 0 646 1406 3576
Size: 646 760 2170
Fragments arranged by size:
2170 760 646
AhdI GACnn_n'nnGTC
Cuts at: 0 1832 3576
Size: 1832 1744
AleI CACnn'nnGTG
Cuts at: 0 1123 3576
Size: 1123 2453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3300 3576
Size: 3300 276
AlwNI CAG_nnn'CTG
Cuts at: 0 622 768 873 909 1095 1446 1531 1721
Size: 622 146 105 36 186 351 85 190
Cuts at: 1721 1962 2280 3054 3576
Size: 241 318 774 522
Fragments arranged by size:
774 622 522 351 318 241 190 186
146 105 85 36
ApaLI G'TGCA_C
Cuts at: 0 136 1475 2879 3576
Size: 136 1339 1404 697
Fragments arranged by size:
1404 1339 697 136
ApoI r'AATT_y
Cuts at: 0 310 3240 3576
Size: 310 2930 336
Fragments arranged by size:
2930 336 310
AvaI C'yCGr_G
Cuts at: 0 34 107 608 1470 1783 1809 2299 2416
Size: 34 73 501 862 313 26 490 117
Cuts at: 2416 3576
Size: 1160
Fragments arranged by size:
1160 862 501 490 313 117 73 34
26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 942 975 3576
Size: 942 33 2601
Fragments arranged by size:
2601 942 33
BanI G'GyrC_C
Cuts at: 0 833 950 1363 1378 1779 2338 2602 3555
Size: 833 117 413 15 401 559 264 953
Cuts at: 3555 3568 3576
Size: 13 8
Fragments arranged by size:
953 833 559 413 401 264 117 15
13 8
BanII G_rGCy'C
Cuts at: 0 1182 2505 3576
Size: 1182 1323 1071
Fragments arranged by size:
1323 1182 1071
BbeI G_GCGC'C
Cuts at: 0 2342 3576
Size: 2342 1234
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 1797 3139 3382 3576
Size: 806 991 1342 243 194
Fragments arranged by size:
1342 991 806 243 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1740 1774 2171 2205 3576
Size: 1740 34 397 34 1371
Fragments arranged by size:
1740 1371 397 34 34
BclI T'GATC_A
Cuts at: 0 2267 3576
Size: 2267 1309
BglI GCCn_nnn'nGGC
Cuts at: 0 842 1777 2725 3576
Size: 842 935 948 851
Fragments arranged by size:
948 935 851 842
BglII A'GATC_T
Cuts at: 0 1839 3083 3576
Size: 1839 1244 493
Fragments arranged by size:
1839 1244 493
BlpI GC'TnA_GC
Cuts at: 0 2285 3576
Size: 2285 1291
Bme1580I G_kGCm'C
Cuts at: 0 140 1381 1479 1567 2605 2819 2883 3560
Size: 140 1241 98 88 1038 214 64 677
Cuts at: 3560 3576
Size: 16
Fragments arranged by size:
1241 1038 677 214 140 98 88 64
16
BmgBI CAC'GTC
Cuts at: 0 649 2598 2943 3312 3576
Size: 649 1949 345 369 264
Fragments arranged by size:
1949 649 369 345 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 919 1731 3576
Size: 919 812 1845
Fragments arranged by size:
1845 919 812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2038 3576
Size: 2038 1538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 266 711 2356 2895 3576
Size: 190 76 445 1645 539 681
Fragments arranged by size:
1645 681 539 445 190 76
Bpu10I CC'TnA_GC
Cuts at: 0 7 2672 3576
Size: 7 2665 904
Fragments arranged by size:
2665 904 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2136 3576
Size: 2136 1440
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 3576
Size: 97 3479
BsaAI yAC'GTr
Cuts at: 0 1407 2814 3147 3576
Size: 1407 1407 333 429
Fragments arranged by size:
1407 1407 429 333
BsaHI Gr'CG_yC
Cuts at: 0 1058 1394 2339 3422 3576
Size: 1058 336 945 1083 154
Fragments arranged by size:
1083 1058 945 336 154
BsaWI w'CCGG_w
Cuts at: 0 3371 3576
Size: 3371 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2516 2546 3576
Size: 2516 30 1030
Fragments arranged by size:
2516 1030 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 55 511 982 2275 2317 2840 3199
Size: 52 3 456 471 1293 42 523 359
Cuts at: 3199 3330 3337 3576
Size: 131 7 239
Fragments arranged by size:
1293 523 471 456 359 239 131 52
42 7 3
BseYI C'CCAG_C
Cuts at: 0 117 527 885 1275 1772 2556 2720 2909
Size: 117 410 358 390 497 784 164 189
Cuts at: 2909 2949 3548 3560 3576
Size: 40 599 12 16
Fragments arranged by size:
784 599 497 410 390 358 189 164
117 40 16 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1744 1975 2770 3316 3576
Size: 922 822 231 795 546 260
Fragments arranged by size:
922 822 795 546 260 231
BsiEI CG_ry'CG
Cuts at: 0 23 1124 3576
Size: 23 1101 2452
Fragments arranged by size:
2452 1101 23
BsiHKAI G_wGCw'C
Cuts at: 0 140 1479 2757 2883 3152 3464 3576
Size: 140 1339 1278 126 269 312 112
Fragments arranged by size:
1339 1278 312 269 140 126 112
BsmI GAATG_Cn'
Cuts at: 0 3032 3576
Size: 3032 544
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1307 2441 3576
Size: 1307 1134 1135
Fragments arranged by size:
1307 1135 1134
Bsp1286I G_dGCh'C
Cuts at: 0 140 1182 1381 1479 1567 2505 2605 2757
Size: 140 1042 199 98 88 938 100 152
Cuts at: 2757 2819 2883 3152 3464 3560 3576
Size: 62 64 269 312 96 16
Fragments arranged by size:
1042 938 312 269 199 152 140 100
98 96 88 64 62 16
BspEI T'CCGG_A
Cuts at: 0 3371 3576
Size: 3371 205
BspHI T'CATG_A
Cuts at: 0 369 2024 3576
Size: 369 1655 1552
Fragments arranged by size:
1655 1552 369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 487 2367 3576
Size: 487 1880 1209
Fragments arranged by size:
1880 1209 487
BsrBI CCG'CTC
Cuts at: 0 174 3576
Size: 174 3402
BsrDI GCAATG_nn'
Cuts at: 0 2116 2462 3403 3576
Size: 2116 346 941 173
Fragments arranged by size:
2116 941 346 173
BsrFI r'CCGG_y
Cuts at: 0 18 1660 2366 3576
Size: 18 1642 706 1210
Fragments arranged by size:
1642 1210 706 18
BsrGI T'GTAC_A
Cuts at: 0 294 1895 3576
Size: 294 1601 1681
Fragments arranged by size:
1681 1601 294
BssSI C'ACGA_G
Cuts at: 0 1309 3576
Size: 1309 2267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1531 1882 1953 2708 3576
Size: 1531 351 71 755 868
Fragments arranged by size:
1531 868 755 351 71
BstEII G'GTnAC_C
Cuts at: 0 1701 3576
Size: 1701 1875
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1182 1726 2485 2637 2917 3576
Size: 1182 544 759 152 280 659
Fragments arranged by size:
1182 759 659 544 280 152
BstYI r'GATC_y
Cuts at: 0 1624 1839 2244 2640 3083 3576
Size: 1624 215 405 396 443 493
Fragments arranged by size:
1624 493 443 405 396 215
Bsu36I CC'TnA_GG
Cuts at: 0 829 1439 3576
Size: 829 610 2137
Fragments arranged by size:
2137 829 610
BtgI C'CryG_G
Cuts at: 0 23 746 2579 3156 3179 3380 3576
Size: 23 723 1833 577 23 201 196
Fragments arranged by size:
1833 723 577 201 196 23 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3258 3576
Size: 3258 318
BtsI GCAGTG_nn'
Cuts at: 0 1958 2042 2217 3284 3470 3500 3576
Size: 1958 84 175 1067 186 30 76
Fragments arranged by size:
1958 1067 186 175 84 76 30
DraI TTT'AAA
Cuts at: 0 309 783 3576
Size: 309 474 2793
Fragments arranged by size:
2793 474 309
DraIII CAC_nnn'GTG
Cuts at: 0 3476 3576
Size: 3476 100
EaeI y'GGCC_r
Cuts at: 0 20 3383 3576
Size: 20 3363 193
Fragments arranged by size:
3363 193 20
EagI C'GGCC_G
Cuts at: 0 20 3576
Size: 20 3556
EarI CTCTTCn'nnn_
Cuts at: 0 1515 3576
Size: 1515 2061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 61 1201 3576
Size: 61 1140 2375
Fragments arranged by size:
2375 1140 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 266 711 946 1126 1203 1354 1468
Size: 190 76 445 235 180 77 151 114
Cuts at: 1468 1984 2356 2895 3048 3151 3576
Size: 516 372 539 153 103 425
Fragments arranged by size:
539 516 445 425 372 235 190 180
153 151 114 103 77 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 997 3576
Size: 130 867 2579
Fragments arranged by size:
2579 867 130
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1959 3051 3071 3324 3545 3576
Size: 765 1194 1092 20 253 221 31
Fragments arranged by size:
1194 1092 765 253 221 31 20
EcoRI G'AATT_C
Cuts at: 0 3240 3576
Size: 3240 336
EcoRV GAT'ATC
Cuts at: 0 3252 3576
Size: 3252 324
FspI TGC'GCA
Cuts at: 0 1875 3576
Size: 1875 1701
HaeII r_GCGC'y
Cuts at: 0 79 115 1267 1342 1759 1796 2342 3576
Size: 79 36 1152 75 417 37 546 1234
Fragments arranged by size:
1234 1152 546 417 79 75 37 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 1818 1824 1850 1856 2848 2880
Size: 262 32 1524 6 26 6 992 32
Cuts at: 2880 3100 3132 3576
Size: 220 32 444
Fragments arranged by size:
1524 992 444 262 220 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 2987 3576
Size: 2987 589
HindIII A'AGCT_T
Cuts at: 0 434 895 3576
Size: 434 461 2681
Fragments arranged by size:
2681 461 434
KasI G'GCGC_C
Cuts at: 0 2338 3576
Size: 2338 1238
KpnI G_GTAC'C
Cuts at: 0 954 3576
Size: 954 2622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 236 1652 3576
Size: 200 36 1416 1924
Fragments arranged by size:
1924 1416 200 36
MslI CAynn'nnrTG
Cuts at: 0 745 1123 1724 2693 3277 3576
Size: 745 378 601 969 584 299
Fragments arranged by size:
969 745 601 584 378 299
MspA1I CmG'CkG
Cuts at: 0 25 619 1092 1600 3068 3126 3336 3366
Size: 25 594 473 508 1468 58 210 30
Cuts at: 3366 3552 3576
Size: 186 24
Fragments arranged by size:
1468 594 508 473 210 186 58 30
25 24
NaeI GCC'GGC
Cuts at: 0 1662 2368 3576
Size: 1662 706 1208
Fragments arranged by size:
1662 1208 706
NarI GG'CG_CC
Cuts at: 0 2339 3576
Size: 2339 1237
NcoI C'CATG_G
Cuts at: 0 746 3576
Size: 746 2830
NgoMIV G'CCGG_C
Cuts at: 0 1660 2366 3576
Size: 1660 706 1210
Fragments arranged by size:
1660 1210 706
NspI r_CATG'y
Cuts at: 0 456 3576
Size: 456 3120
PasI CC'CwG_GG
Cuts at: 0 2967 3054 3576
Size: 2967 87 522
Fragments arranged by size:
2967 522 87
PfoI T'CCnGG_A
Cuts at: 0 683 1226 1541 2440 3576
Size: 683 543 315 899 1136
Fragments arranged by size:
1136 899 683 543 315
PmlI CAC'GTG
Cuts at: 0 2814 3147 3576
Size: 2814 333 429
Fragments arranged by size:
2814 429 333
PpuMI rG'GwC_Cy
Cuts at: 0 3071 3576
Size: 3071 505
PsiI TTA'TAA
Cuts at: 0 1645 3576
Size: 1645 1931
PstI C_TGCA'G
Cuts at: 0 764 850 989 1140 1372 1519 3083 3335
Size: 764 86 139 151 232 147 1564 252
Cuts at: 3335 3576
Size: 241
Fragments arranged by size:
1564 764 252 241 232 151 147 139
86
PvuII CAG'CTG
Cuts at: 0 619 1092 1600 3336 3552 3576
Size: 619 473 508 1736 216 24
Fragments arranged by size:
1736 619 508 473 216 24
SacII CC_GC'GG
Cuts at: 0 26 3576
Size: 26 3550
SalI G'TCGA_C
Cuts at: 0 2985 3576
Size: 2985 591
SanDI GG'GwC_CC
Cuts at: 0 3071 3576
Size: 3071 505
SapI GCTCTTCn'nnn_
Cuts at: 0 1515 3576
Size: 1515 2061
SexAI A'CCwGG_T
Cuts at: 0 1697 3576
Size: 1697 1879
SfcI C'TryA_G
Cuts at: 0 760 846 985 1136 1368 1515 3079 3331
Size: 760 86 139 151 232 147 1564 252
Cuts at: 3331 3414 3576
Size: 83 162
Fragments arranged by size:
1564 760 252 232 162 151 147 139
86 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2725 3576
Size: 2725 851
SfoI GGC'GCC
Cuts at: 0 2340 3576
Size: 2340 1236
SmaI CCC'GGG
Cuts at: 0 109 3576
Size: 109 3467
SmlI C'TyrA_G
Cuts at: 0 1470 2151 3576
Size: 1470 681 1425
Fragments arranged by size:
1470 1425 681
SspI AAT'ATT
Cuts at: 0 779 2218 3576
Size: 779 1439 1358
Fragments arranged by size:
1439 1358 779
StuI AGG'CCT
Cuts at: 0 1289 2893 3576
Size: 1289 1604 683
Fragments arranged by size:
1604 1289 683
StyI C'CwwG_G
Cuts at: 0 329 422 746 1142 2333 2916 3576
Size: 329 93 324 396 1191 583 660
Fragments arranged by size:
1191 660 583 396 329 324 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 541 1048 1517 2923 3019 3169 3576
Size: 541 507 469 1406 96 150 407
Fragments arranged by size:
1406 541 507 469 407 150 96
TatI w'GTAC_w
Cuts at: 0 294 1673 1895 3020 3576
Size: 294 1379 222 1125 556
Fragments arranged by size:
1379 1125 556 294 222
Tth111I GACn'n_nGTC
Cuts at: 0 1057 1120 2982 3576
Size: 1057 63 1862 594
Fragments arranged by size:
1862 1057 594 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1635 2856 3549 3576
Size: 1635 1221 693 27
Fragments arranged by size:
1635 1221 693 27
XhoI C'TCGA_G
Cuts at: 0 1470 3576
Size: 1470 2106
XmaI C'CCGG_G
Cuts at: 0 107 3576
Size: 107 3469
ZraI GAC'GTC
Cuts at: 0 1059 3423 3576
Size: 1059 2364 153
Fragments arranged by size:
2364 1059 153
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbeI
BbsI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PfoI PmlI PpuMI
PsiI PstI PvuII SacII SalI SanDI SapI SexAI
SfcI SfiI SfoI SmaI SmlI SspI StuI StyI
TaqII TatI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AloI ApaI AscI
AseI AsiSI AvrII BamHI BbvCI BciVI BfrBI BmtI
BsaBI BsiWI BssHII BstBI BstZ17I ClaI CspCI DrdI
EcoICRI FalI FseI FspAI HpaI MfeI MluI MscI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TstI XbaI XmnI