Restriction map for
pF1KSDA0989
(Linear) (Six-base) MAPSORT of: KIAA0989.seq Check: 3222 from: 1 to: 2340
KIAA0989.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 879 1573 2227 2340
Size: 879 694 654 113
Fragments arranged by size:
879 694 654 113
Acc65I G'GTAC_C
Cuts at: 0 689 2340
Size: 689 1651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 746 1921 2340
Size: 472 274 1175 419
Fragments arranged by size:
1175 472 419 274
AfeI AGC'GCT
Cuts at: 0 1945 2340
Size: 1945 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 2340
Size: 365 1975
AlwNI CAG_nnn'CTG
Cuts at: 0 114 195 792 1383 1677 1908 2340
Size: 114 81 597 591 294 231 432
Fragments arranged by size:
597 591 432 294 231 114 81
ApaI G_GGCC'C
Cuts at: 0 408 522 580 595 610 2340
Size: 408 114 58 15 15 1730
Fragments arranged by size:
1730 408 114 58 15 15
ApaLI G'TGCA_C
Cuts at: 0 1565 2340
Size: 1565 775
AvaI C'yCGr_G
Cuts at: 0 21 408 515 617 915 1409 1477 2340
Size: 21 387 107 102 298 494 68 863
Fragments arranged by size:
863 494 387 298 107 102 68 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 619 652 2340
Size: 619 33 1688
Fragments arranged by size:
1688 619 33
BamHI G'GATC_C
Cuts at: 0 734 2340
Size: 734 1606
BanI G'GyrC_C
Cuts at: 0 511 689 750 1336 1585 2001 2340
Size: 511 178 61 586 249 416 339
Fragments arranged by size:
586 511 416 339 249 178 61
BanII G_rGCy'C
Cuts at: 0 151 221 408 522 538 580 595 610
Size: 151 70 187 114 16 42 15 15
Cuts at: 610 859 1012 1628 1981 2054 2340
Size: 249 153 616 353 73 286
Fragments arranged by size:
616 353 286 249 187 153 151 114
73 70 42 16 15 15
BbeI G_GCGC'C
Cuts at: 0 515 1340 2340
Size: 515 825 1000
Fragments arranged by size:
1000 825 515
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1126 2340
Size: 20 1106 1214
Fragments arranged by size:
1214 1106 20
BbvCI CC'TCA_GC
Cuts at: 0 448 602 906 952 2340
Size: 448 154 304 46 1388
Fragments arranged by size:
1388 448 304 154 46
BclI T'GATC_A
Cuts at: 0 1925 2340
Size: 1925 415
BglI GCCn_nnn'nGGC
Cuts at: 0 361 931 2340
Size: 361 570 1409
Fragments arranged by size:
1409 570 361
BlpI GC'TnA_GC
Cuts at: 0 1627 1775 2340
Size: 1627 148 565
Fragments arranged by size:
1627 565 148
Bme1580I G_kGCm'C
Cuts at: 0 408 461 522 580 595 610 699 897
Size: 408 53 61 58 15 15 89 198
Cuts at: 897 927 1569 1590 2340
Size: 30 642 21 750
Fragments arranged by size:
750 642 408 198 89 61 58 53
30 21 15 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 2340
Size: 143 2197
BmtI G_CTAG'C
Cuts at: 0 97 2340
Size: 97 2243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 520 1024 1135 1330 1393 1537 1561
Size: 184 336 504 111 195 63 144 24
Cuts at: 1561 1604 1705 1930 1932 2340
Size: 43 101 225 2 408
Fragments arranged by size:
504 408 336 225 195 184 144 111
101 63 43 24 2
Bpu10I CC'TnA_GC
Cuts at: 0 448 602 853 906 952 2340
Size: 448 154 251 53 46 1388
Fragments arranged by size:
1388 448 251 154 53 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1537 2340
Size: 1537 803
BsaI GGTCTCn'nnnn_
Cuts at: 0 655 1622 1949 2340
Size: 655 967 327 391
Fragments arranged by size:
967 655 391 327
BsaAI yAC'GTr
Cuts at: 0 462 2340
Size: 462 1878
BsaBI GATnn'nnATC
Cuts at: 0 1194 2335 2340
Size: 1194 1141 5
Fragments arranged by size:
1194 1141 5
BsaHI Gr'CG_yC
Cuts at: 0 512 1337 2081 2340
Size: 512 825 744 259
Fragments arranged by size:
825 744 512 259
BsaWI w'CCGG_w
Cuts at: 0 2229 2340
Size: 2229 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 520 550 1699 1729 2197 2227 2340
Size: 520 30 1149 30 468 30 113
Fragments arranged by size:
1149 520 468 113 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 9 319 337 528 894 1086 1167 1435
Size: 9 310 18 191 366 192 81 268
Cuts at: 1435 1681 2137 2340
Size: 246 456 203
Fragments arranged by size:
456 366 310 268 246 203 192 191
81 18 9
BseYI C'CCAG_C
Cuts at: 0 292 356 523 1808 1819 2340
Size: 292 64 167 1285 11 521
Fragments arranged by size:
1285 521 292 167 64 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 2274 2340
Size: 19 2255 66
Fragments arranged by size:
2255 66 19
BsiHKAI G_wGCw'C
Cuts at: 0 236 538 812 859 1012 1569 1628 1895
Size: 236 302 274 47 153 557 59 267
Cuts at: 1895 2115 2185 2340
Size: 220 70 155
Fragments arranged by size:
557 302 274 267 236 220 155 153
70 59 47
BsmI GAATG_Cn'
Cuts at: 0 966 2340
Size: 966 1374
BsmBI CGTCTCn'nnnn_
Cuts at: 0 73 2340
Size: 73 2267
Bsp1286I G_dGCh'C
Cuts at: 0 151 221 236 408 461 522 538 580
Size: 151 70 15 172 53 61 16 42
Cuts at: 580 595 610 699 812 859 897 927 1012
Size: 15 15 89 113 47 38 30 85
Cuts at: 1012 1569 1590 1628 1895 1981 2054 2115 2185
Size: 557 21 38 267 86 73 61 70
Cuts at: 2185 2340
Size: 155
Fragments arranged by size:
557 267 172 155 151 113 89 86
85 73 70 70 61 61 53 47
42 38 38 30 21 16 15 15
15
BspHI T'CATG_A
Cuts at: 0 657 2340
Size: 657 1683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 800 879 1573 1871 2227 2340
Size: 800 79 694 298 356 113
Fragments arranged by size:
800 694 356 298 113 79
BsrBI CCG'CTC
Cuts at: 0 987 1461 2340
Size: 987 474 879
Fragments arranged by size:
987 879 474
BsrDI GCAATG_nn'
Cuts at: 0 1395 2340
Size: 1395 945
BsrFI r'CCGG_y
Cuts at: 0 275 1240 2340
Size: 275 965 1100
Fragments arranged by size:
1100 965 275
BstYI r'GATC_y
Cuts at: 0 167 393 734 1176 1673 1904 2340
Size: 167 226 341 442 497 231 436
Fragments arranged by size:
497 442 436 341 231 226 167
Bsu36I CC'TnA_GG
Cuts at: 0 115 2340
Size: 115 2225
BtgI C'CryG_G
Cuts at: 0 399 685 699 843 1748 1763 2340
Size: 399 286 14 144 905 15 577
Fragments arranged by size:
905 577 399 286 144 15 14
BtsI GCAGTG_nn'
Cuts at: 0 668 927 1324 1608 1614 1928 2340
Size: 668 259 397 284 6 314 412
Fragments arranged by size:
668 412 397 314 284 259 6
EaeI y'GGCC_r
Cuts at: 0 373 840 1026 1259 1854 2340
Size: 373 467 186 233 595 486
Fragments arranged by size:
595 486 467 373 233 186
EarI CTCTTCn'nnn_
Cuts at: 0 1412 2061 2340
Size: 1412 649 279
Fragments arranged by size:
1412 649 279
EcoICRI GAG'CTC
Cuts at: 0 536 857 1010 1626 2340
Size: 536 321 153 616 714
Fragments arranged by size:
714 616 536 321 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 472 520 746 1024 1135 1330 1393
Size: 184 288 48 226 278 111 195 63
Cuts at: 1393 1537 1561 1604 1705 1921 1930 1932 2340
Size: 144 24 43 101 216 9 2 408
Fragments arranged by size:
408 288 278 226 216 195 184 144
111 101 63 48 43 24 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 1543 1912 2340
Size: 46 1497 369 428
Fragments arranged by size:
1497 428 369 46
EcoO109I rG'GnC_Cy
Cuts at: 0 111 368 404 405 435 519 576 577
Size: 111 257 36 1 30 84 57 1
Cuts at: 577 591 592 606 622 1110 1557 1932 2340
Size: 14 1 14 16 488 447 375 408
Fragments arranged by size:
488 447 408 375 257 111 84 57
36 30 16 14 14 1 1 1
FspI TGC'GCA
Cuts at: 0 1394 1429 2340
Size: 1394 35 911
Fragments arranged by size:
1394 911 35
HaeII r_GCGC'y
Cuts at: 0 515 1340 1477 1634 1684 1947 2340
Size: 515 825 137 157 50 263 393
Fragments arranged by size:
825 515 393 263 157 137 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2197 2229 2340
Size: 2197 32 111
Fragments arranged by size:
2197 111 32
KasI G'GCGC_C
Cuts at: 0 511 1336 2340
Size: 511 825 1004
Fragments arranged by size:
1004 825 511
KpnI G_GTAC'C
Cuts at: 0 693 2340
Size: 693 1647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 792 1821 2340
Size: 400 392 1029 519
Fragments arranged by size:
1029 519 400 392
MscI TGG'CCA
Cuts at: 0 1261 1856 2340
Size: 1261 595 484
Fragments arranged by size:
1261 595 484
MspA1I CmG'CkG
Cuts at: 0 57 558 701 832 1059 1353 1485 1515
Size: 57 501 143 131 227 294 132 30
Cuts at: 1515 2340
Size: 825
Fragments arranged by size:
825 501 294 227 143 132 131 57
30
NaeI GCC'GGC
Cuts at: 0 1242 2340
Size: 1242 1098
NarI GG'CG_CC
Cuts at: 0 512 1337 2340
Size: 512 825 1003
Fragments arranged by size:
1003 825 512
NcoI C'CATG_G
Cuts at: 0 843 1748 2340
Size: 843 905 592
Fragments arranged by size:
905 843 592
NgoMIV G'CCGG_C
Cuts at: 0 1240 2340
Size: 1240 1100
NheI G'CTAG_C
Cuts at: 0 93 2340
Size: 93 2247
NspI r_CATG'y
Cuts at: 0 382 724 2340
Size: 382 342 1616
Fragments arranged by size:
1616 382 342
PasI CC'CwG_GG
Cuts at: 0 222 2037 2340
Size: 222 1815 303
Fragments arranged by size:
1815 303 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 1908 2340
Size: 1908 432
PfoI T'CCnGG_A
Cuts at: 0 47 1907 1948 2306 2340
Size: 47 1860 41 358 34
Fragments arranged by size:
1860 358 47 41 34
PmlI CAC'GTG
Cuts at: 0 462 2340
Size: 462 1878
PpuMI rG'GwC_Cy
Cuts at: 0 368 622 1932 2340
Size: 368 254 1310 408
Fragments arranged by size:
1310 408 368 254
PspOMI G'GGCC_C
Cuts at: 0 404 518 576 591 606 2340
Size: 404 114 58 15 15 1734
Fragments arranged by size:
1734 404 114 58 15 15
PspXI vC'TCGA_Gb
Cuts at: 0 1409 2340
Size: 1409 931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 123 155 256 288 2340
Size: 123 32 101 32 2052
Fragments arranged by size:
2052 123 101 32 32
PstI C_TGCA'G
Cuts at: 0 200 743 797 974 995 1469 1490 2340
Size: 200 543 54 177 21 474 21 850
Fragments arranged by size:
850 543 474 200 177 54 21 21
PvuII CAG'CTG
Cuts at: 0 57 558 832 1485 1515 2340
Size: 57 501 274 653 30 825
Fragments arranged by size:
825 653 501 274 57 30
SacI G_AGCT'C
Cuts at: 0 538 859 1012 1628 2340
Size: 538 321 153 616 712
Fragments arranged by size:
712 616 538 321 153
SacII CC_GC'GG
Cuts at: 0 702 2340
Size: 702 1638
SanDI GG'GwC_CC
Cuts at: 0 368 2340
Size: 368 1972
SbfI CC_TGCA'GG
Cuts at: 0 743 2340
Size: 743 1597
ScaI AGT'ACT
Cuts at: 0 346 2340
Size: 346 1994
SfcI C'TryA_G
Cuts at: 0 196 739 793 970 991 1465 1486 2099
Size: 196 543 54 177 21 474 21 613
Cuts at: 2099 2288 2340
Size: 189 52
Fragments arranged by size:
613 543 474 196 189 177 54 52
21 21
SfoI GGC'GCC
Cuts at: 0 513 1338 2340
Size: 513 825 1002
Fragments arranged by size:
1002 825 513
SmaI CCC'GGG
Cuts at: 0 410 517 2340
Size: 410 107 1823
Fragments arranged by size:
1823 410 107
SmlI C'TyrA_G
Cuts at: 0 1409 1552 2340
Size: 1409 143 788
Fragments arranged by size:
1409 788 143
SphI G_CATG'C
Cuts at: 0 724 2340
Size: 724 1616
SrfI GCCC'GGGC
Cuts at: 0 517 2340
Size: 517 1823
StuI AGG'CCT
Cuts at: 0 157 901 1441 2340
Size: 157 744 540 899
Fragments arranged by size:
899 744 540 157
StyI C'CwwG_G
Cuts at: 0 843 1748 2340
Size: 843 905 592
Fragments arranged by size:
905 843 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 1833 2130 2200 2340
Size: 404 1429 297 70 140
Fragments arranged by size:
1429 404 297 140 70
TatI w'GTAC_w
Cuts at: 0 344 648 2340
Size: 344 304 1692
Fragments arranged by size:
1692 344 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 520 552 2340
Size: 520 32 1788
Fragments arranged by size:
1788 520 32
XhoI C'TCGA_G
Cuts at: 0 1409 2340
Size: 1409 931
XmaI C'CCGG_G
Cuts at: 0 408 515 2340
Size: 408 107 1825
Fragments arranged by size:
1825 408 107
Enzymes that do cut:
AarI Acc65I AcuI AfeI AlfI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstYI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SbfI ScaI SfcI SfoI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BcgI BciVI
BfrBI BglII BmgBI BplI BsiEI BsiWI BspEI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI
MluI MslI NdeI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PvuI RsrII SalI SapI
SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI
Tth111I XbaI XcmI XmnI ZraI