Restriction map for
pF1KSDA0990
(Linear) (Six-base) MAPSORT of: KIAA0990.seq Check: 9127 from: 1 to: 2406
KIAA0990.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AatII G_ACGT'C
Cuts at: 0 1895 2406
Size: 1895 511
AccI GT'mk_AC
Cuts at: 0 1895 2406
Size: 1895 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1552 1906 1962 2406
Size: 121 1431 354 56 444
Fragments arranged by size:
1431 444 354 121 56
AflIII A'CryG_T
Cuts at: 0 518 1409 1634 1669 2086 2406
Size: 518 891 225 35 417 320
Fragments arranged by size:
891 518 417 320 225 35
AlwNI CAG_nnn'CTG
Cuts at: 0 2328 2406
Size: 2328 78
ApaI G_GGCC'C
Cuts at: 0 115 2406
Size: 115 2291
ApoI r'AATT_y
Cuts at: 0 887 1043 1915 2406
Size: 887 156 872 491
Fragments arranged by size:
887 872 491 156
AscI GG'CGCG_CC
Cuts at: 0 18 2406
Size: 18 2388
AvaI C'yCGr_G
Cuts at: 0 40 86 133 299 2406
Size: 40 46 47 166 2107
Fragments arranged by size:
2107 166 47 46 40
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 368 401 2406
Size: 368 33 2005
Fragments arranged by size:
2005 368 33
BamHI G'GATC_C
Cuts at: 0 1504 1849 2406
Size: 1504 345 557
Fragments arranged by size:
1504 557 345
BanI G'GyrC_C
Cuts at: 0 119 604 705 2406
Size: 119 485 101 1701
Fragments arranged by size:
1701 485 119 101
BanII G_rGCy'C
Cuts at: 0 47 115 578 980 1833 2406
Size: 47 68 463 402 853 573
Fragments arranged by size:
853 573 463 402 68 47
BbeI G_GCGC'C
Cuts at: 0 123 2406
Size: 123 2283
BbsI GAAGACnn'nnnn_
Cuts at: 0 1672 2203 2406
Size: 1672 531 203
Fragments arranged by size:
1672 531 203
BclI T'GATC_A
Cuts at: 0 674 1244 2406
Size: 674 570 1162
Fragments arranged by size:
1162 674 570
BglI GCCn_nnn'nGGC
Cuts at: 0 137 178 2406
Size: 137 41 2228
Fragments arranged by size:
2228 137 41
BglII A'GATC_T
Cuts at: 0 870 2103 2406
Size: 870 1233 303
Fragments arranged by size:
1233 870 303
BlpI GC'TnA_GC
Cuts at: 0 28 958 2406
Size: 28 930 1448
Fragments arranged by size:
1448 930 28
Bme1580I G_kGCm'C
Cuts at: 0 115 607 2406
Size: 115 492 1799
Fragments arranged by size:
1799 492 115
BmgBI CAC'GTC
Cuts at: 0 519 756 1670 2232 2406
Size: 519 237 914 562 174
Fragments arranged by size:
914 562 519 237 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 1851 2015 2406
Size: 1851 164 391
Fragments arranged by size:
1851 391 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 655 1045 1835 2082 2173 2406
Size: 562 93 390 790 247 91 233
Fragments arranged by size:
790 562 390 247 233 93 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 442 2406
Size: 345 97 1964
Fragments arranged by size:
1964 345 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 527 2406
Size: 527 1879
BsaAI yAC'GTr
Cuts at: 0 2087 2406
Size: 2087 319
BsaBI GATnn'nnATC
Cuts at: 0 2258 2406
Size: 2258 148
BsaHI Gr'CG_yC
Cuts at: 0 120 1892 2406
Size: 120 1772 514
Fragments arranged by size:
1772 514 120
BsaWI w'CCGG_w
Cuts at: 0 776 2406
Size: 776 1630
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2093 2123 2406
Size: 2093 30 283
Fragments arranged by size:
2093 283 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 570 1126 1416 1471 2406
Size: 570 556 290 55 935
Fragments arranged by size:
935 570 556 290 55
BseYI C'CCAG_C
Cuts at: 0 1065 2144 2406
Size: 1065 1079 262
Fragments arranged by size:
1079 1065 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 985 2406
Size: 814 171 1421
Fragments arranged by size:
1421 814 171
BsiEI CG_ry'CG
Cuts at: 0 15 2406
Size: 15 2391
BsiHKAI G_wGCw'C
Cuts at: 0 980 1577 2406
Size: 980 597 829
Fragments arranged by size:
980 829 597
Bsp1286I G_dGCh'C
Cuts at: 0 47 115 578 607 980 1577 1833 2406
Size: 47 68 463 29 373 597 256 573
Fragments arranged by size:
597 573 463 373 256 68 47 29
BspEI T'CCGG_A
Cuts at: 0 776 2406
Size: 776 1630
BspHI T'CATG_A
Cuts at: 0 272 449 677 1085 2406
Size: 272 177 228 408 1321
Fragments arranged by size:
1321 408 272 228 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 1221 1358 2179 2406
Size: 299 922 137 821 227
Fragments arranged by size:
922 821 299 227 137
BsrBI CCG'CTC
Cuts at: 0 109 2406
Size: 109 2297
BsrFI r'CCGG_y
Cuts at: 0 14 143 172 2406
Size: 14 129 29 2234
Fragments arranged by size:
2234 129 29 14
BsrGI T'GTAC_A
Cuts at: 0 521 737 1361 2406
Size: 521 216 624 1045
Fragments arranged by size:
1045 624 521 216
BssHII G'CGCG_C
Cuts at: 0 7 18 181 193 2406
Size: 7 11 163 12 2213
Fragments arranged by size:
2213 163 12 11 7
BssSI C'ACGA_G
Cuts at: 0 1699 2406
Size: 1699 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1803 2406
Size: 1803 603
BstYI r'GATC_y
Cuts at: 0 870 1292 1504 1849 2103 2406
Size: 870 422 212 345 254 303
Fragments arranged by size:
870 422 345 303 254 212
Bsu36I CC'TnA_GG
Cuts at: 0 553 2406
Size: 553 1853
BtgI C'CryG_G
Cuts at: 0 115 305 608 2406
Size: 115 190 303 1798
Fragments arranged by size:
1798 303 190 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 203 970 1941 2406
Size: 203 767 971 465
Fragments arranged by size:
971 767 465 203
BtsI GCAGTG_nn'
Cuts at: 0 802 2406
Size: 802 1604
DrdI GACnn_nn'nnGTC
Cuts at: 0 1222 2313 2406
Size: 1222 1091 93
Fragments arranged by size:
1222 1091 93
EaeI y'GGCC_r
Cuts at: 0 2 12 211 308 1167 1478 2406
Size: 2 10 199 97 859 311 928
Fragments arranged by size:
928 859 311 199 97 10 2
EagI C'GGCC_G
Cuts at: 0 12 2406
Size: 12 2394
EarI CTCTTCn'nnn_
Cuts at: 0 265 1176 1561 1885 2406
Size: 265 911 385 324 521
Fragments arranged by size:
911 521 385 324 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 970 1185 2406
Size: 970 215 1221
Fragments arranged by size:
1221 970 215
EcoICRI GAG'CTC
Cuts at: 0 978 2406
Size: 978 1428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 562 655 1045 1552 1835 1906 1962
Size: 121 441 93 390 507 283 71 56
Cuts at: 1962 2082 2173 2406
Size: 120 91 233
Fragments arranged by size:
507 441 390 283 233 121 120 93
91 71 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1086 1181 2406
Size: 1086 95 1225
Fragments arranged by size:
1225 1086 95
EcoO109I rG'GnC_Cy
Cuts at: 0 664 1056 2406
Size: 664 392 1350
Fragments arranged by size:
1350 664 392
EcoRI G'AATT_C
Cuts at: 0 1915 2406
Size: 1915 491
EcoRV GAT'ATC
Cuts at: 0 971 2406
Size: 971 1435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 2406
Size: 462 1944
HaeII r_GCGC'y
Cuts at: 0 123 2406
Size: 123 2283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1476 1508 2093 2125 2406
Size: 1259 32 185 32 585 32 281
Fragments arranged by size:
1259 585 281 185 32 32 32
HincII GTy'rAC
Cuts at: 0 1164 1896 2406
Size: 1164 732 510
Fragments arranged by size:
1164 732 510
KasI G'GCGC_C
Cuts at: 0 119 2406
Size: 119 2287
MluI A'CGCG_T
Cuts at: 0 1409 2406
Size: 1409 997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1751 2406
Size: 1751 655
MscI TGG'CCA
Cuts at: 0 1480 2406
Size: 1480 926
MslI CAynn'nnrTG
Cuts at: 0 1225 1240 1639 2406
Size: 1225 15 399 767
Fragments arranged by size:
1225 767 399 15
MspA1I CmG'CkG
Cuts at: 0 999 1065 2328 2406
Size: 999 66 1263 78
Fragments arranged by size:
1263 999 78 66
NaeI GCC'GGC
Cuts at: 0 16 174 2406
Size: 16 158 2232
Fragments arranged by size:
2232 158 16
NarI GG'CG_CC
Cuts at: 0 120 2406
Size: 120 2286
NgoMIV G'CCGG_C
Cuts at: 0 14 172 2406
Size: 14 158 2234
Fragments arranged by size:
2234 158 14
NspI r_CATG'y
Cuts at: 0 468 957 1638 1797 2406
Size: 468 489 681 159 609
Fragments arranged by size:
681 609 489 468 159
PasI CC'CwG_GG
Cuts at: 0 1558 2406
Size: 1558 848
PciI A'CATG_T
Cuts at: 0 1634 2406
Size: 1634 772
PflMI CCAn_nnn'nTGG
Cuts at: 0 480 1446 2406
Size: 480 966 960
Fragments arranged by size:
966 960 480
PfoI T'CCnGG_A
Cuts at: 0 728 1343 2406
Size: 728 615 1063
Fragments arranged by size:
1063 728 615
PmlI CAC'GTG
Cuts at: 0 2087 2406
Size: 2087 319
PpuMI rG'GwC_Cy
Cuts at: 0 1056 2406
Size: 1056 1350
PsiI TTA'TAA
Cuts at: 0 2096 2406
Size: 2096 310
PspOMI G'GGCC_C
Cuts at: 0 111 2406
Size: 111 2295
PstI C_TGCA'G
Cuts at: 0 296 2406
Size: 296 2110
PvuII CAG'CTG
Cuts at: 0 999 1065 2328 2406
Size: 999 66 1263 78
Fragments arranged by size:
1263 999 78 66
RsrII CG'GwC_CG
Cuts at: 0 146 2406
Size: 146 2260
SacI G_AGCT'C
Cuts at: 0 980 2406
Size: 980 1426
SalI G'TCGA_C
Cuts at: 0 1894 2406
Size: 1894 512
SapI GCTCTTCn'nnn_
Cuts at: 0 1885 2406
Size: 1885 521
SfcI C'TryA_G
Cuts at: 0 292 315 1910 2406
Size: 292 23 1595 496
Fragments arranged by size:
1595 496 292 23
SfoI GGC'GCC
Cuts at: 0 121 2406
Size: 121 2285
SmaI CCC'GGG
Cuts at: 0 88 2406
Size: 88 2318
SmlI C'TyrA_G
Cuts at: 0 360 457 2406
Size: 360 97 1949
Fragments arranged by size:
1949 360 97
StuI AGG'CCT
Cuts at: 0 599 2406
Size: 599 1807
StyI C'CwwG_G
Cuts at: 0 1211 2139 2406
Size: 1211 928 267
Fragments arranged by size:
1211 928 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 2107 2406
Size: 760 1347 299
Fragments arranged by size:
1347 760 299
TatI w'GTAC_w
Cuts at: 0 461 521 737 935 1337 1361 2276 2406
Size: 461 60 216 198 402 24 915 130
Fragments arranged by size:
915 461 402 216 198 130 60 24
Tth111I GACn'n_nGTC
Cuts at: 0 1222 1900 2406
Size: 1222 678 506
Fragments arranged by size:
1222 678 506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 2325 2406
Size: 615 1710 81
Fragments arranged by size:
1710 615 81
XmaI C'CCGG_G
Cuts at: 0 86 2406
Size: 86 2320
XmnI GAAnn'nnTTC
Cuts at: 0 445 1919 2406
Size: 445 1474 487
Fragments arranged by size:
1474 487 445
ZraI GAC'GTC
Cuts at: 0 1893 2406
Size: 1893 513
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI ApoI AscI
AvaI BaeI BamHI BanI BanII BbeI BbsI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII KasI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PsiI PspOMI PstI PvuII RsrII SacI SalI
SapI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AseI AsiSI AvrII BbvCI BcgI
BciVI BfrBI BmtI BplI Bpu10I BsiWI BsmI BsmBI
BsrDI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI
DraIII FseI FspI FspAI HindIII HpaI KpnI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PshAI PspXI PsrI PvuI SacII SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XhoI