Restriction map for
pF1KSDA0992
(Linear) (Six-base) MAPSORT of: KIAA0992.seq Check: 8210 from: 1 to: 2016
KIAA0992.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 669 1783 2016
Size: 669 1114 233
Fragments arranged by size:
1114 669 233
Acc65I G'GTAC_C
Cuts at: 0 1610 2016
Size: 1610 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1248 2016
Size: 270 978 768
Fragments arranged by size:
978 768 270
AfeI AGC'GCT
Cuts at: 0 432 2016
Size: 432 1584
AflIII A'CryG_T
Cuts at: 0 317 928 1486 2016
Size: 317 611 558 530
Fragments arranged by size:
611 558 530 317
ApaI G_GGCC'C
Cuts at: 0 101 2016
Size: 101 1915
ApaLI G'TGCA_C
Cuts at: 0 1435 2016
Size: 1435 581
ApoI r'AATT_y
Cuts at: 0 554 2016
Size: 554 1462
AvaI C'yCGr_G
Cuts at: 0 94 229 351 357 2016
Size: 94 135 122 6 1659
Fragments arranged by size:
1659 135 122 94 6
BanI G'GyrC_C
Cuts at: 0 309 864 1610 2016
Size: 309 555 746 406
Fragments arranged by size:
746 555 406 309
BanII G_rGCy'C
Cuts at: 0 101 230 247 358 2016
Size: 101 129 17 111 1658
Fragments arranged by size:
1658 129 111 101 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 2016
Size: 276 1740
BbvCI CC'TCA_GC
Cuts at: 0 427 1896 2016
Size: 427 1469 120
Fragments arranged by size:
1469 427 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1901 1935 2016
Size: 1901 34 81
Fragments arranged by size:
1901 81 34
BclI T'GATC_A
Cuts at: 0 1002 1445 2016
Size: 1002 443 571
Fragments arranged by size:
1002 571 443
BglII A'GATC_T
Cuts at: 0 899 959 986 1023 1293 1359 2016
Size: 899 60 27 37 270 66 657
Fragments arranged by size:
899 657 270 66 60 37 27
BlpI GC'TnA_GC
Cuts at: 0 224 2016
Size: 224 1792
Bme1580I G_kGCm'C
Cuts at: 0 101 314 1439 2016
Size: 101 213 1125 577
Fragments arranged by size:
1125 577 213 101
BmgBI CAC'GTC
Cuts at: 0 318 1463 2016
Size: 318 1145 553
Fragments arranged by size:
1145 553 318
BmrI ACTGGGnnnn_n'
Cuts at: 0 1609 1842 2016
Size: 1609 233 174
Fragments arranged by size:
1609 233 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 735 1310 1549 2016
Size: 111 624 575 239 467
Fragments arranged by size:
624 575 467 239 111
Bpu10I CC'TnA_GC
Cuts at: 0 427 1896 2016
Size: 427 1469 120
Fragments arranged by size:
1469 427 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 806 1181 2016
Size: 806 375 835
Fragments arranged by size:
835 806 375
BsaAI yAC'GTr
Cuts at: 0 1468 2016
Size: 1468 548
BsaHI Gr'CG_yC
Cuts at: 0 169 367 412 2016
Size: 169 198 45 1604
Fragments arranged by size:
1604 198 169 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 332 362 733 763 2016
Size: 332 30 371 30 1253
Fragments arranged by size:
1253 371 332 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 240 2016
Size: 237 3 1776
Fragments arranged by size:
1776 237 3
BseYI C'CCAG_C
Cuts at: 0 133 1774 2016
Size: 133 1641 242
Fragments arranged by size:
1641 242 133
BsiEI CG_ry'CG
Cuts at: 0 186 464 2016
Size: 186 278 1552
Fragments arranged by size:
1552 278 186
BsiHKAI G_wGCw'C
Cuts at: 0 1407 1439 1873 2016
Size: 1407 32 434 143
Fragments arranged by size:
1407 434 143 32
BsiWI C'GTAC_G
Cuts at: 0 1389 2016
Size: 1389 627
BsmI GAATG_Cn'
Cuts at: 0 917 2016
Size: 917 1099
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1226 2016
Size: 1226 790
Bsp1286I G_dGCh'C
Cuts at: 0 101 230 247 314 358 1407 1439 1873
Size: 101 129 17 67 44 1049 32 434
Cuts at: 1873 2016
Size: 143
Fragments arranged by size:
1049 434 143 129 101 67 44 32
17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1045 2016
Size: 1045 971
BsrBI CCG'CTC
Cuts at: 0 20 348 2016
Size: 20 328 1668
Fragments arranged by size:
1668 328 20
BsrFI r'CCGG_y
Cuts at: 0 120 456 1474 2016
Size: 120 336 1018 542
Fragments arranged by size:
1018 542 336 120
BssHII G'CGCG_C
Cuts at: 0 5 61 2016
Size: 5 56 1955
Fragments arranged by size:
1955 56 5
BstEII G'GTnAC_C
Cuts at: 0 1346 2016
Size: 1346 670
BstYI r'GATC_y
Cuts at: 0 899 959 986 1023 1293 1359 2016
Size: 899 60 27 37 270 66 657
Fragments arranged by size:
899 657 270 66 60 37 27
BstZ17I GTA'TAC
Cuts at: 0 1784 2016
Size: 1784 232
Bsu36I CC'TnA_GG
Cuts at: 0 1106 1182 1958 2016
Size: 1106 76 776 58
Fragments arranged by size:
1106 776 76 58
BtgI C'CryG_G
Cuts at: 0 111 238 293 402 416 1728 2016
Size: 111 127 55 109 14 1312 288
Fragments arranged by size:
1312 288 127 111 109 55 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 63 2016
Size: 63 1953
BtsI GCAGTG_nn'
Cuts at: 0 325 372 2016
Size: 325 47 1644
Fragments arranged by size:
1644 325 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1323 1358 2016
Size: 1323 35 658
Fragments arranged by size:
1323 658 35
DraI TTT'AAA
Cuts at: 0 972 2016
Size: 972 1044
DraIII CAC_nnn'GTG
Cuts at: 0 1850 2016
Size: 1850 166
DrdI GACnn_nn'nnGTC
Cuts at: 0 1336 2016
Size: 1336 680
EaeI y'GGCC_r
Cuts at: 0 183 399 405 461 630 2016
Size: 183 216 6 56 169 1386
Fragments arranged by size:
1386 216 183 169 56 6
EagI C'GGCC_G
Cuts at: 0 183 461 2016
Size: 183 278 1555
Fragments arranged by size:
1555 278 183
EarI CTCTTCn'nnn_
Cuts at: 0 713 2016
Size: 713 1303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 12 2016
Size: 12 2004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 270 735 1248 1310 1549 2016
Size: 111 159 465 513 62 239 467
Fragments arranged by size:
513 467 465 239 159 111 62
EcoO109I rG'GnC_Cy
Cuts at: 0 234 615 1034 2010 2016
Size: 234 381 419 976 6
Fragments arranged by size:
976 419 381 234 6
HaeII r_GCGC'y
Cuts at: 0 224 434 2016
Size: 224 210 1582
Fragments arranged by size:
1582 224 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1922 1954 2016
Size: 1922 32 62
Fragments arranged by size:
1922 62 32
HincII GTy'rAC
Cuts at: 0 468 644 670 1152 2016
Size: 468 176 26 482 864
Fragments arranged by size:
864 482 468 176 26
HpaI GTT'AAC
Cuts at: 0 644 2016
Size: 644 1372
KpnI G_GTAC'C
Cuts at: 0 1614 2016
Size: 1614 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1977 2016
Size: 1977 39
MscI TGG'CCA
Cuts at: 0 632 2016
Size: 632 1384
MslI CAynn'nnrTG
Cuts at: 0 1193 2016
Size: 1193 823
MspA1I CmG'CkG
Cuts at: 0 418 755 2016
Size: 418 337 1261
Fragments arranged by size:
1261 418 337
NaeI GCC'GGC
Cuts at: 0 122 458 1476 2016
Size: 122 336 1018 540
Fragments arranged by size:
1018 540 336 122
NgoMIV G'CCGG_C
Cuts at: 0 120 456 1474 2016
Size: 120 336 1018 542
Fragments arranged by size:
1018 542 336 120
NspI r_CATG'y
Cuts at: 0 932 1490 1716 2016
Size: 932 558 226 300
Fragments arranged by size:
932 558 300 226
PasI CC'CwG_GG
Cuts at: 0 474 2016
Size: 474 1542
PciI A'CATG_T
Cuts at: 0 928 1486 2016
Size: 928 558 530
Fragments arranged by size:
928 558 530
PfoI T'CCnGG_A
Cuts at: 0 1287 2016
Size: 1287 729
PmlI CAC'GTG
Cuts at: 0 1468 2016
Size: 1468 548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 784 2016
Size: 752 32 1232
Fragments arranged by size:
1232 752 32
PpuMI rG'GwC_Cy
Cuts at: 0 615 1034 2010 2016
Size: 615 419 976 6
Fragments arranged by size:
976 615 419 6
PshAI GACnn'nnGTC
Cuts at: 0 366 2016
Size: 366 1650
PspOMI G'GGCC_C
Cuts at: 0 97 2016
Size: 97 1919
PstI C_TGCA'G
Cuts at: 0 1324 2016
Size: 1324 692
PvuII CAG'CTG
Cuts at: 0 755 2016
Size: 755 1261
SacII CC_GC'GG
Cuts at: 0 419 2016
Size: 419 1597
SalI G'TCGA_C
Cuts at: 0 668 2016
Size: 668 1348
SfcI C'TryA_G
Cuts at: 0 1320 2016
Size: 1320 696
SgrAI Cr'CCGG_yG
Cuts at: 0 456 2016
Size: 456 1560
SmaI CCC'GGG
Cuts at: 0 359 2016
Size: 359 1657
SphI G_CATG'C
Cuts at: 0 1716 2016
Size: 1716 300
SspI AAT'ATT
Cuts at: 0 705 2016
Size: 705 1311
StuI AGG'CCT
Cuts at: 0 1206 2016
Size: 1206 810
StyI C'CwwG_G
Cuts at: 0 485 1994 2016
Size: 485 1509 22
Fragments arranged by size:
1509 485 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1718 1766 1861 2016
Size: 1718 48 95 155
Fragments arranged by size:
1718 155 95 48
TatI w'GTAC_w
Cuts at: 0 785 1123 1822 2016
Size: 785 338 699 194
Fragments arranged by size:
785 699 338 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 2016
Size: 332 32 1652
Fragments arranged by size:
1652 332 32
XbaI T'CTAG_A
Cuts at: 0 1213 2016
Size: 1213 803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 962 1364 2016
Size: 962 402 652
Fragments arranged by size:
962 652 402
XmaI C'CCGG_G
Cuts at: 0 357 2016
Size: 357 1659
XmnI GAAnn'nnTTC
Cuts at: 0 1247 1519 2016
Size: 1247 272 497
Fragments arranged by size:
1247 497 272
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII
Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PciI PfoI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacII SalI SfcI
SgrAI SmaI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BglI BmtI BplI BpuEI BsaBI
BsaWI BsgI BspEI BspHI BsrDI BsrGI BssSI BstAPI
BstBI BstXI ClaI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII KasI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PsiI PspXI PsrI PvuI RsrII SacI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI
SnaBI SpeI SrfI SwaI Tth111I XhoI ZraI