Restriction map for
pF1KA0994
(Linear) (Six-base) MAPSORT of: KIAA0994.seq Check: 9884 from: 1 to: 1995
KIAA0994.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1353 1995
Size: 1353 642
AccI GT'mk_AC
Cuts at: 0 86 515 1995
Size: 86 429 1480
Fragments arranged by size:
1480 429 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 145 1024 1372 1995
Size: 102 43 879 348 623
Fragments arranged by size:
879 623 348 102 43
AfeI AGC'GCT
Cuts at: 0 202 1636 1995
Size: 202 1434 359
Fragments arranged by size:
1434 359 202
AflIII A'CryG_T
Cuts at: 0 138 140 539 1995
Size: 138 2 399 1456
Fragments arranged by size:
1456 399 138 2
AleI CACnn'nnGTG
Cuts at: 0 1853 1995
Size: 1853 142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 1995
Size: 244 1751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 643 675 730 762 1995
Size: 643 32 55 32 1233
Fragments arranged by size:
1233 643 55 32 32
ApaI G_GGCC'C
Cuts at: 0 111 430 1162 1995
Size: 111 319 732 833
Fragments arranged by size:
833 732 319 111
ApoI r'AATT_y
Cuts at: 0 1456 1918 1995
Size: 1456 462 77
Fragments arranged by size:
1456 462 77
AvaI C'yCGr_G
Cuts at: 0 430 1115 1181 1995
Size: 430 685 66 814
Fragments arranged by size:
814 685 430 66
BamHI G'GATC_C
Cuts at: 0 1043 1995
Size: 1043 952
BanI G'GyrC_C
Cuts at: 0 64 1371 1942 1989 1995
Size: 64 1307 571 47 6
Fragments arranged by size:
1307 571 64 47 6
BanII G_rGCy'C
Cuts at: 0 111 332 430 1154 1162 1190 1385 1871
Size: 111 221 98 724 8 28 195 486
Cuts at: 1871 1995
Size: 124
Fragments arranged by size:
724 486 221 195 124 111 98 28
8
BbvCI CC'TCA_GC
Cuts at: 0 173 1995
Size: 173 1822
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 297 331 1272 1306 1875 1888 1909 1922
Size: 297 34 941 34 569 13 21 13
Cuts at: 1922 1995
Size: 73
Fragments arranged by size:
941 569 297 73 34 34 21 13
13
BciVI GTATCCnnnnn_n'
Cuts at: 0 583 1995
Size: 583 1412
BglI GCCn_nnn'nGGC
Cuts at: 0 434 1812 1995
Size: 434 1378 183
Fragments arranged by size:
1378 434 183
BglII A'GATC_T
Cuts at: 0 350 528 1995
Size: 350 178 1467
Fragments arranged by size:
1467 350 178
Bme1580I G_kGCm'C
Cuts at: 0 67 111 430 1162 1994 1995
Size: 67 44 319 732 832 1
Fragments arranged by size:
832 732 319 67 44 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 1240 1995
Size: 1240 755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 801 1404 1995
Size: 801 603 591
Fragments arranged by size:
801 603 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1252 1861 1885 1995
Size: 838 414 609 24 110
Fragments arranged by size:
838 609 414 110 24
Bpu10I CC'TnA_GC
Cuts at: 0 173 1995
Size: 173 1822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1648 1995
Size: 517 1131 347
Fragments arranged by size:
1131 517 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 801 1995
Size: 801 1194
BsaAI yAC'GTr
Cuts at: 0 57 141 648 1176 1413 1851 1995
Size: 57 84 507 528 237 438 144
Fragments arranged by size:
528 507 438 237 144 84 57
BsaWI w'CCGG_w
Cuts at: 0 875 1995
Size: 875 1120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 643 673 730 760 1609 1639
Size: 37 30 576 30 57 30 849 30
Cuts at: 1639 1995
Size: 356
Fragments arranged by size:
849 576 356 57 37 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 1840 1995
Size: 190 1650 155
Fragments arranged by size:
1650 190 155
BseYI C'CCAG_C
Cuts at: 0 60 99 217 1358 1674 1995
Size: 60 39 118 1141 316 321
Fragments arranged by size:
1141 321 316 118 60 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1633 1995
Size: 1387 246 362
Fragments arranged by size:
1387 362 246
BsiEI CG_ry'CG
Cuts at: 0 576 1038 1995
Size: 576 462 957
Fragments arranged by size:
957 576 462
BsiHKAI G_wGCw'C
Cuts at: 0 256 1154 1385 1995
Size: 256 898 231 610
Fragments arranged by size:
898 610 256 231
BsmI GAATG_Cn'
Cuts at: 0 308 1837 1888 1995
Size: 308 1529 51 107
Fragments arranged by size:
1529 308 107 51
Bsp1286I G_dGCh'C
Cuts at: 0 67 111 256 332 430 1154 1162 1190
Size: 67 44 145 76 98 724 8 28
Cuts at: 1190 1385 1871 1994 1995
Size: 195 486 123 1
Fragments arranged by size:
724 486 195 145 123 98 76 67
44 28 8 1
BspEI T'CCGG_A
Cuts at: 0 875 1995
Size: 875 1120
BspHI T'CATG_A
Cuts at: 0 893 1995
Size: 893 1102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1353 1712 1995
Size: 1353 359 283
Fragments arranged by size:
1353 359 283
BsrBI CCG'CTC
Cuts at: 0 12 1711 1995
Size: 12 1699 284
Fragments arranged by size:
1699 284 12
BsrFI r'CCGG_y
Cuts at: 0 692 1995
Size: 692 1303
BsrGI T'GTAC_A
Cuts at: 0 605 1995
Size: 605 1390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1989 1995
Size: 1989 6
BstEII G'GTnAC_C
Cuts at: 0 234 276 1995
Size: 234 42 1719
Fragments arranged by size:
1719 234 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1995
Size: 453 1542
BstYI r'GATC_y
Cuts at: 0 350 528 1043 1995
Size: 350 178 515 952
Fragments arranged by size:
952 515 350 178
BtgI C'CryG_G
Cuts at: 0 734 886 1097 1376 1524 1995
Size: 734 152 211 279 148 471
Fragments arranged by size:
734 471 279 211 152 148
BtsI GCAGTG_nn'
Cuts at: 0 273 1995
Size: 273 1722
DraIII CAC_nnn'GTG
Cuts at: 0 1989 1995
Size: 1989 6
EaeI y'GGCC_r
Cuts at: 0 182 666 1278 1472 1521 1995
Size: 182 484 612 194 49 474
Fragments arranged by size:
612 484 474 194 182 49
EarI CTCTTCn'nnn_
Cuts at: 0 337 1504 1995
Size: 337 1167 491
Fragments arranged by size:
1167 491 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 711 1995
Size: 711 1284
EcoICRI GAG'CTC
Cuts at: 0 1152 1383 1995
Size: 1152 231 612
Fragments arranged by size:
1152 612 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 145 838 1024 1252 1372 1861 1885
Size: 102 43 693 186 228 120 489 24
Cuts at: 1885 1995
Size: 110
Fragments arranged by size:
693 489 228 186 120 110 102 43
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 964 1612 1666 1995
Size: 964 648 54 329
Fragments arranged by size:
964 648 329 54
EcoO109I rG'GnC_Cy
Cuts at: 0 107 426 427 568 1077 1788 1995
Size: 107 319 1 141 509 711 207
Fragments arranged by size:
711 509 319 207 141 107 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1029 1995
Size: 1029 966
HaeII r_GCGC'y
Cuts at: 0 204 1638 1995
Size: 204 1434 357
Fragments arranged by size:
1434 357 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1411 1443 1520 1552 1995
Size: 1411 32 77 32 443
Fragments arranged by size:
1411 443 77 32 32
HincII GTy'rAC
Cuts at: 0 285 1995
Size: 285 1710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1300 1622 1995
Size: 1021 279 322 373
Fragments arranged by size:
1021 373 322 279
MscI TGG'CCA
Cuts at: 0 184 1474 1995
Size: 184 1290 521
Fragments arranged by size:
1290 521 184
MslI CAynn'nnrTG
Cuts at: 0 613 1415 1774 1853 1995
Size: 613 802 359 79 142
Fragments arranged by size:
802 613 359 142 79
MspA1I CmG'CkG
Cuts at: 0 736 1995
Size: 736 1259
NaeI GCC'GGC
Cuts at: 0 694 1995
Size: 694 1301
NcoI C'CATG_G
Cuts at: 0 1524 1995
Size: 1524 471
NgoMIV G'CCGG_C
Cuts at: 0 692 1995
Size: 692 1303
NspI r_CATG'y
Cuts at: 0 543 1995
Size: 543 1452
PasI CC'CwG_GG
Cuts at: 0 1872 1995
Size: 1872 123
PciI A'CATG_T
Cuts at: 0 539 1995
Size: 539 1456
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 1168 1995
Size: 687 481 827
Fragments arranged by size:
827 687 481
PfoI T'CCnGG_A
Cuts at: 0 415 1046 1862 1995
Size: 415 631 816 133
Fragments arranged by size:
816 631 415 133
PmlI CAC'GTG
Cuts at: 0 141 1413 1851 1995
Size: 141 1272 438 144
Fragments arranged by size:
1272 438 144 141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 730 762 1995
Size: 643 32 55 32 1233
Fragments arranged by size:
1233 643 55 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1788 1995
Size: 1788 207
PshAI GACnn'nnGTC
Cuts at: 0 82 1995
Size: 82 1913
PspOMI G'GGCC_C
Cuts at: 0 107 426 1158 1995
Size: 107 319 732 837
Fragments arranged by size:
837 732 319 107
PstI C_TGCA'G
Cuts at: 0 29 1995
Size: 29 1966
PvuI CG_AT'CG
Cuts at: 0 1038 1995
Size: 1038 957
RsrII CG'GwC_CG
Cuts at: 0 14 1995
Size: 14 1981
SacI G_AGCT'C
Cuts at: 0 1154 1385 1995
Size: 1154 231 610
Fragments arranged by size:
1154 610 231
SacII CC_GC'GG
Cuts at: 0 737 1995
Size: 737 1258
SapI GCTCTTCn'nnn_
Cuts at: 0 337 1504 1995
Size: 337 1167 491
Fragments arranged by size:
1167 491 337
SfcI C'TryA_G
Cuts at: 0 25 87 516 1995
Size: 25 62 429 1479
Fragments arranged by size:
1479 429 62 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 434 1995
Size: 434 1561
SmaI CCC'GGG
Cuts at: 0 1183 1995
Size: 1183 812
SmlI C'TyrA_G
Cuts at: 0 532 1663 1995
Size: 532 1131 332
Fragments arranged by size:
1131 532 332
StuI AGG'CCT
Cuts at: 0 761 791 1995
Size: 761 30 1204
Fragments arranged by size:
1204 761 30
StyI C'CwwG_G
Cuts at: 0 488 1331 1524 1995
Size: 488 843 193 471
Fragments arranged by size:
843 488 471 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 135 482 1543 1995
Size: 94 41 347 1061 452
Fragments arranged by size:
1061 452 347 94 41
TatI w'GTAC_w
Cuts at: 0 605 719 821 1625 1995
Size: 605 114 102 804 370
Fragments arranged by size:
804 605 370 114 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 1995
Size: 127 32 1836
Fragments arranged by size:
1836 127 32
Tth111I GACn'n_nGTC
Cuts at: 0 274 1995
Size: 274 1721
XmaI C'CCGG_G
Cuts at: 0 1181 1995
Size: 1181 814
XmnI GAAnn'nnTTC
Cuts at: 0 658 1963 1995
Size: 658 1305 32
Fragments arranged by size:
1305 658 32
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
ApaI ApoI AvaI BamHI BanI BanII BbvCI BcgI
BciVI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI RsrII
SacI SacII SapI SfcI SfiI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BclI
BfrBI BlpI BmgBI BmtI BsaBI BsaHI BsiWI BsmBI
BsrDI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PmeI PsiI
PspXI PsrI PvuII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XcmI XhoI ZraI