Restriction map for
pF1KA1000
(Linear) (Six-base) MAPSORT of: KIAA1000.seq Check: 9215 from: 1 to: 5838
KIAA1000.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4929 5034 5838
Size: 4929 105 804
Fragments arranged by size:
4929 804 105
AccI GT'mk_AC
Cuts at: 0 2082 5838
Size: 2082 3756
Acc65I G'GTAC_C
Cuts at: 0 1791 5838
Size: 1791 4047
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1034 2282 2546 2611 2686 2692 3130
Size: 388 646 1248 264 65 75 6 438
Cuts at: 3130 3331 3352 4865 4897 4957 5056 5062 5279
Size: 201 21 1513 32 60 99 6 217
Cuts at: 5279 5362 5450 5632 5838
Size: 83 88 182 206
Fragments arranged by size:
1513 1248 646 438 388 264 217 206
201 182 99 88 83 75 65 60
32 21 6 6
AfeI AGC'GCT
Cuts at: 0 3799 5838
Size: 3799 2039
AflIII A'CryG_T
Cuts at: 0 1511 5838
Size: 1511 4327
AhdI GACnn_n'nnGTC
Cuts at: 0 4320 5838
Size: 4320 1518
AleI CACnn'nnGTG
Cuts at: 0 794 2320 5838
Size: 794 1526 3518
Fragments arranged by size:
3518 1526 794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 812 2440 5838
Size: 812 1628 3398
Fragments arranged by size:
3398 1628 812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1700 1732 5838
Size: 1700 32 4106
Fragments arranged by size:
4106 1700 32
AlwNI CAG_nnn'CTG
Cuts at: 0 991 3054 3555 3684 5838
Size: 991 2063 501 129 2154
Fragments arranged by size:
2154 2063 991 501 129
ApaI G_GGCC'C
Cuts at: 0 1374 5838
Size: 1374 4464
ApaLI G'TGCA_C
Cuts at: 0 2603 5838
Size: 2603 3235
ApoI r'AATT_y
Cuts at: 0 775 902 1123 1750 2434 2832 3306 3544
Size: 775 127 221 627 684 398 474 238
Cuts at: 3544 4788 5838
Size: 1244 1050
Fragments arranged by size:
1244 1050 775 684 627 474 398 238
221 127
AseI AT'TA_AT
Cuts at: 0 1469 5838
Size: 1469 4369
AvaI C'yCGr_G
Cuts at: 0 3419 3643 4309 4330 5838
Size: 3419 224 666 21 1508
Fragments arranged by size:
3419 1508 666 224 21
BamHI G'GATC_C
Cuts at: 0 3499 5838
Size: 3499 2339
BanI G'GyrC_C
Cuts at: 0 796 1309 1791 3142 4289 5838
Size: 796 513 482 1351 1147 1549
Fragments arranged by size:
1549 1351 1147 796 513 482
BanII G_rGCy'C
Cuts at: 0 1374 3644 4310 4478 4686 5153 5556 5838
Size: 1374 2270 666 168 208 467 403 282
Fragments arranged by size:
2270 1374 666 467 403 282 208 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1862 2379 3358 3980 4193 4651 5609
Size: 323 1539 517 979 622 213 458 958
Cuts at: 5609 5838
Size: 229
Fragments arranged by size:
1539 979 958 622 517 458 323 229
213
BbvCI CC'TCA_GC
Cuts at: 0 197 3274 5206 5838
Size: 197 3077 1932 632
Fragments arranged by size:
3077 1932 632 197
BciVI GTATCCnnnnn_n'
Cuts at: 0 2679 5838
Size: 2679 3159
BfrBI ATG'CAT
Cuts at: 0 2051 5573 5838
Size: 2051 3522 265
Fragments arranged by size:
3522 2051 265
BglI GCCn_nnn'nGGC
Cuts at: 0 5305 5317 5838
Size: 5305 12 521
Fragments arranged by size:
5305 521 12
BglII A'GATC_T
Cuts at: 0 1599 5838
Size: 1599 4239
BlpI GC'TnA_GC
Cuts at: 0 113 2714 3085 3341 3512 3638 4043 4100
Size: 113 2601 371 256 171 126 405 57
Cuts at: 4100 4921 5057 5838
Size: 821 136 781
Fragments arranged by size:
2601 821 781 405 371 256 171 136
126 113 57
Bme1580I G_kGCm'C
Cuts at: 0 1314 1374 2607 3147 4020 5838
Size: 1314 60 1233 540 873 1818
Fragments arranged by size:
1818 1314 1233 873 540 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 2292 5838
Size: 2292 3546
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3466 5838
Size: 3466 2372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 893 1115 1543 1708 2800 3220 3505
Size: 281 612 222 428 165 1092 420 285
Cuts at: 3505 4324 4900 5245 5533 5838
Size: 819 576 345 288 305
Fragments arranged by size:
1092 819 612 576 428 420 345 305
288 285 281 222 165
Bpu10I CC'TnA_GC
Cuts at: 0 197 3094 3274 3758 5206 5838
Size: 197 2897 180 484 1448 632
Fragments arranged by size:
2897 1448 632 484 197 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1459 1474 3157 3169 4465 4471 5584 5765
Size: 1459 15 1683 12 1296 6 1113 181
Cuts at: 5765 5838
Size: 73
Fragments arranged by size:
1683 1459 1296 1113 181 73 15 12
6
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 3994 5838
Size: 415 3579 1844
Fragments arranged by size:
3579 1844 415
BsaBI GATnn'nnATC
Cuts at: 0 2428 4981 5838
Size: 2428 2553 857
Fragments arranged by size:
2553 2428 857
BsaHI Gr'CG_yC
Cuts at: 0 4316 5838
Size: 4316 1522
BsaWI w'CCGG_w
Cuts at: 0 4109 5838
Size: 4109 1729
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 2564 2594 3870 3900 5838
Size: 958 30 1576 30 1276 30 1938
Fragments arranged by size:
1938 1576 1276 958 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 750 1200 2839 3088 3091 3502 4432 4468
Size: 750 450 1639 249 3 411 930 36
Cuts at: 4468 4516 5194 5838
Size: 48 678 644
Fragments arranged by size:
1639 930 750 678 644 450 411 249
48 36 3
BseYI C'CCAG_C
Cuts at: 0 2335 5838
Size: 2335 3503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3049 3052 3175 3541 3568 4090 5290 5555
Size: 3049 3 123 366 27 522 1200 265
Cuts at: 5555 5838
Size: 283
Fragments arranged by size:
3049 1200 522 366 283 265 123 27
3
BsiHKAI G_wGCw'C
Cuts at: 0 2412 2607 2954 3644 4310 4478 5153 5838
Size: 2412 195 347 690 666 168 675 685
Fragments arranged by size:
2412 690 685 675 666 347 195 168
BsmI GAATG_Cn'
Cuts at: 0 186 1409 2204 5838
Size: 186 1223 795 3634
Fragments arranged by size:
3634 1223 795 186
Bsp1286I G_dGCh'C
Cuts at: 0 1314 1374 2412 2607 2954 3147 3644 4020
Size: 1314 60 1038 195 347 193 497 376
Cuts at: 4020 4310 4478 4686 5153 5556 5838
Size: 290 168 208 467 403 282
Fragments arranged by size:
1314 1038 497 467 403 376 347 290
282 208 195 193 168 60
BspHI T'CATG_A
Cuts at: 0 927 5838
Size: 927 4911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 941 3071 3660 4929 5034 5838
Size: 941 2130 589 1269 105 804
Fragments arranged by size:
2130 1269 941 804 589 105
BsrBI CCG'CTC
Cuts at: 0 4343 5061 5838
Size: 4343 718 777
Fragments arranged by size:
4343 777 718
BsrDI GCAATG_nn'
Cuts at: 0 332 4150 5360 5838
Size: 332 3818 1210 478
Fragments arranged by size:
3818 1210 478 332
BsrFI r'CCGG_y
Cuts at: 0 4934 5838
Size: 4934 904
BssSI C'ACGA_G
Cuts at: 0 4414 5838
Size: 4414 1424
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4296 5838
Size: 4296 1542
BstEII G'GTnAC_C
Cuts at: 0 1293 4866 5838
Size: 1293 3573 972
Fragments arranged by size:
3573 1293 972
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 871 4251 4962 5838
Size: 141 730 3380 711 876
Fragments arranged by size:
3380 876 730 711 141
BstYI r'GATC_y
Cuts at: 0 63 1599 2771 3499 5094 5838
Size: 63 1536 1172 728 1595 744
Fragments arranged by size:
1595 1536 1172 744 728 63
BstZ17I GTA'TAC
Cuts at: 0 2083 5838
Size: 2083 3755
Bsu36I CC'TnA_GG
Cuts at: 0 1658 5838
Size: 1658 4180
BtgI C'CryG_G
Cuts at: 0 1040 4244 5838
Size: 1040 3204 1594
Fragments arranged by size:
3204 1594 1040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5583 5838
Size: 5583 255
BtsI GCAGTG_nn'
Cuts at: 0 2752 4813 5538 5838
Size: 2752 2061 725 300
Fragments arranged by size:
2752 2061 725 300
ClaI AT'CG_AT
Cuts at: 0 2897 5838
Size: 2897 2941
DraI TTT'AAA
Cuts at: 0 1128 2190 2331 3212 5838
Size: 1128 1062 141 881 2626
Fragments arranged by size:
2626 1128 1062 881 141
EaeI y'GGCC_r
Cuts at: 0 381 470 1025 4253 5127 5838
Size: 381 89 555 3228 874 711
Fragments arranged by size:
3228 874 711 555 381 89
EarI CTCTTCn'nnn_
Cuts at: 0 418 481 583 1139 1637 2536 2591 2735
Size: 418 63 102 556 498 899 55 144
Cuts at: 2735 2816 3263 3566 3610 3788 3905 4550 4634
Size: 81 447 303 44 178 117 645 84
Cuts at: 4634 4886 5078 5141 5255 5285 5838
Size: 252 192 63 114 30 553
Fragments arranged by size:
899 645 556 553 498 447 418 303
252 192 178 144 117 114 102 84
81 63 63 55 44 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2871 5704 5838
Size: 2871 2833 134
Fragments arranged by size:
2871 2833 134
EcoICRI GAG'CTC
Cuts at: 0 3642 4308 4476 5151 5838
Size: 3642 666 168 675 687
Fragments arranged by size:
3642 687 675 666 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 388 893 1034 1115 1543 1708 2282
Size: 281 107 505 141 81 428 165 574
Cuts at: 2282 2546 2611 2686 2692 2800 3130 3220 3331
Size: 264 65 75 6 108 330 90 111
Cuts at: 3331 3352 3505 4324 4865 4897 4900 4957 5056
Size: 21 153 819 541 32 3 57 99
Cuts at: 5056 5062 5245 5279 5362 5450 5533 5632 5838
Size: 6 183 34 83 88 83 99 206
Fragments arranged by size:
819 574 541 505 428 330 281 264
206 183 165 153 141 111 108 107
99 99 90 88 83 83 81 75
65 57 34 32 21 6 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 2523 2677 3151 4036 4333 5838
Size: 343 2180 154 474 885 297 1505
Fragments arranged by size:
2180 1505 885 474 343 297 154
EcoO109I rG'GnC_Cy
Cuts at: 0 29 1370 1371 2219 4389 4896 4989 5102
Size: 29 1341 1 848 2170 507 93 113
Cuts at: 5102 5838
Size: 736
Fragments arranged by size:
2170 1341 848 736 507 113 93 29
1
EcoRI G'AATT_C
Cuts at: 0 3306 5838
Size: 3306 2532
EcoRV GAT'ATC
Cuts at: 0 831 3000 5838
Size: 831 2169 2838
Fragments arranged by size:
2838 2169 831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1852 2287 3720 3972 4765 5838
Size: 1852 435 1433 252 793 1073
Fragments arranged by size:
1852 1433 1073 793 435 252
HaeII r_GCGC'y
Cuts at: 0 378 3801 5838
Size: 378 3423 2037
Fragments arranged by size:
3423 2037 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 745 777 2001 2033 2297 2329
Size: 279 32 434 32 1224 32 264 32
Cuts at: 2329 2362 2394 2532 2564 3991 4023 4306 4338
Size: 33 32 138 32 1427 32 283 32
Cuts at: 4338 4551 4583 5086 5118 5616 5648 5838
Size: 213 32 503 32 498 32 190
Fragments arranged by size:
1427 1224 503 498 434 283 279 264
213 190 138 33 32 32 32 32
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1589 4759 5838
Size: 1589 3170 1079
Fragments arranged by size:
3170 1589 1079
KpnI G_GTAC'C
Cuts at: 0 1795 5838
Size: 1795 4043
MfeI C'AATT_G
Cuts at: 0 1503 5838
Size: 1503 4335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1898 2783 5838
Size: 1898 885 3055
Fragments arranged by size:
3055 1898 885
MscI TGG'CCA
Cuts at: 0 383 1027 4255 5838
Size: 383 644 3228 1583
Fragments arranged by size:
3228 1583 644 383
MslI CAynn'nnrTG
Cuts at: 0 325 341 784 794 2320 2503 4249 5838
Size: 325 16 443 10 1526 183 1746 1589
Fragments arranged by size:
1746 1589 1526 443 325 183 16 10
MspA1I CmG'CkG
Cuts at: 0 2260 2778 3516 3990 4296 4302 4374 5004
Size: 2260 518 738 474 306 6 72 630
Cuts at: 5004 5838
Size: 834
Fragments arranged by size:
2260 834 738 630 518 474 306 72
6
NcoI C'CATG_G
Cuts at: 0 1040 4244 5838
Size: 1040 3204 1594
Fragments arranged by size:
3204 1594 1040
NsiI A_TGCA'T
Cuts at: 0 2053 5575 5838
Size: 2053 3522 263
Fragments arranged by size:
3522 2053 263
NspI r_CATG'y
Cuts at: 0 549 810 1515 3625 5838
Size: 549 261 705 2110 2213
Fragments arranged by size:
2213 2110 705 549 261
PasI CC'CwG_GG
Cuts at: 0 4962 5838
Size: 4962 876
PciI A'CATG_T
Cuts at: 0 1511 5838
Size: 1511 4327
PflMI CCAn_nnn'nTGG
Cuts at: 0 3462 5838
Size: 3462 2376
PfoI T'CCnGG_A
Cuts at: 0 664 5153 5838
Size: 664 4489 685
Fragments arranged by size:
4489 685 664
PpuMI rG'GwC_Cy
Cuts at: 0 2219 4896 4989 5102 5838
Size: 2219 2677 93 113 736
Fragments arranged by size:
2677 2219 736 113 93
PshAI GACnn'nnGTC
Cuts at: 0 4651 5838
Size: 4651 1187
PsiI TTA'TAA
Cuts at: 0 1799 5838
Size: 1799 4039
PspOMI G'GGCC_C
Cuts at: 0 1370 5838
Size: 1370 4468
PspXI vC'TCGA_Gb
Cuts at: 0 3419 3643 5838
Size: 3419 224 2195
Fragments arranged by size:
3419 2195 224
PstI C_TGCA'G
Cuts at: 0 2177 4037 4301 4350 4379 4829 5838
Size: 2177 1860 264 49 29 450 1009
Fragments arranged by size:
2177 1860 1009 450 264 49 29
PvuII CAG'CTG
Cuts at: 0 2260 2778 3516 3990 4296 4302 4374 5004
Size: 2260 518 738 474 306 6 72 630
Cuts at: 5004 5838
Size: 834
Fragments arranged by size:
2260 834 738 630 518 474 306 72
6
SacI G_AGCT'C
Cuts at: 0 3644 4310 4478 5153 5838
Size: 3644 666 168 675 685
Fragments arranged by size:
3644 685 675 666 168
SapI GCTCTTCn'nnn_
Cuts at: 0 1139 2536 2735 3263 3788 5141 5838
Size: 1139 1397 199 528 525 1353 697
Fragments arranged by size:
1397 1353 1139 697 528 525 199
ScaI AGT'ACT
Cuts at: 0 1914 5838
Size: 1914 3924
SexAI A'CCwGG_T
Cuts at: 0 2076 4577 5838
Size: 2076 2501 1261
Fragments arranged by size:
2501 2076 1261
SfcI C'TryA_G
Cuts at: 0 127 1283 1664 2104 2173 2945 3664 4033
Size: 127 1156 381 440 69 772 719 369
Cuts at: 4033 4297 4346 4375 4825 5838
Size: 264 49 29 450 1013
Fragments arranged by size:
1156 1013 772 719 450 440 381 369
264 127 69 49 29
SmlI C'TyrA_G
Cuts at: 0 1438 1453 3136 3148 3419 3643 4480 4486
Size: 1438 15 1683 12 271 224 837 6
Cuts at: 4486 5563 5780 5838
Size: 1077 217 58
Fragments arranged by size:
1683 1438 1077 837 271 224 217 58
15 12 6
SphI G_CATG'C
Cuts at: 0 810 5838
Size: 810 5028
StuI AGG'CCT
Cuts at: 0 3332 5838
Size: 3332 2506
StyI C'CwwG_G
Cuts at: 0 75 140 1040 2215 2279 2621 3023 4244
Size: 75 65 900 1175 64 342 402 1221
Cuts at: 4244 4274 5838
Size: 30 1564
Fragments arranged by size:
1564 1221 1175 900 402 342 75 65
64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1548 3469 5838
Size: 1548 1921 2369
Fragments arranged by size:
2369 1921 1548
TatI w'GTAC_w
Cuts at: 0 1912 3781 4468 5838
Size: 1912 1869 687 1370
Fragments arranged by size:
1912 1869 1370 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 958 990 2136 2168 3870 3902
Size: 593 32 333 32 1146 32 1702 32
Cuts at: 3902 4405 4437 5838
Size: 503 32 1401
Fragments arranged by size:
1702 1401 1146 593 503 333 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1881 2305 2775 4209 5367 5838
Size: 1047 834 424 470 1434 1158 471
Fragments arranged by size:
1434 1158 1047 834 471 470 424
XhoI C'TCGA_G
Cuts at: 0 3419 3643 5838
Size: 3419 224 2195
Fragments arranged by size:
3419 2195 224
XmnI GAAnn'nnTTC
Cuts at: 0 2159 4761 5838
Size: 2159 2602 1077
Fragments arranged by size:
2602 2159 1077
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BciVI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AscI AsiSI AvrII BaeI
BbeI BcgI BclI BmgBI BmtI BsaAI BsiEI BsiWI
BsmBI BspEI BsrGI BssHII BstBI CspCI DraIII DrdI
EagI FseI FspI FspAI HincII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PmeI PmlI PpiI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XmaI ZraI