Restriction map for
pF1KSDA1004
(Linear) (Six-base) MAPSORT of: KIAA1004.seq Check: 848 from: 1 to: 3486
KIAA1004.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 506 859 3486
Size: 506 353 2627
Fragments arranged by size:
2627 506 353
Acc65I G'GTAC_C
Cuts at: 0 46 649 3486
Size: 46 603 2837
Fragments arranged by size:
2837 603 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 2381 3486
Size: 1270 1111 1105
Fragments arranged by size:
1270 1111 1105
AfeI AGC'GCT
Cuts at: 0 1015 2266 3486
Size: 1015 1251 1220
Fragments arranged by size:
1251 1220 1015
AflIII A'CryG_T
Cuts at: 0 512 1855 3486
Size: 512 1343 1631
Fragments arranged by size:
1631 1343 512
AleI CACnn'nnGTG
Cuts at: 0 877 2246 3486
Size: 877 1369 1240
Fragments arranged by size:
1369 1240 877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 913 3486
Size: 913 2573
AlwNI CAG_nnn'CTG
Cuts at: 0 1666 2286 2977 3218 3333 3486
Size: 1666 620 691 241 115 153
Fragments arranged by size:
1666 691 620 241 153 115
ApoI r'AATT_y
Cuts at: 0 237 1057 1567 3053 3486
Size: 237 820 510 1486 433
Fragments arranged by size:
1486 820 510 433 237
AvaI C'yCGr_G
Cuts at: 0 436 2195 3486
Size: 436 1759 1291
Fragments arranged by size:
1759 1291 436
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 641 674 3486
Size: 641 33 2812
Fragments arranged by size:
2812 641 33
BamHI G'GATC_C
Cuts at: 0 267 1317 3486
Size: 267 1050 2169
Fragments arranged by size:
2169 1050 267
BanI G'GyrC_C
Cuts at: 0 46 649 1830 3486
Size: 46 603 1181 1656
Fragments arranged by size:
1656 1181 603 46
BanII G_rGCy'C
Cuts at: 0 809 1297 2429 2655 3296 3486
Size: 809 488 1132 226 641 190
Fragments arranged by size:
1132 809 641 488 226 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 1231 2694 3260 3486
Size: 681 550 1463 566 226
Fragments arranged by size:
1463 681 566 550 226
BbvCI CC'TCA_GC
Cuts at: 0 1075 2713 2983 3486
Size: 1075 1638 270 503
Fragments arranged by size:
1638 1075 503 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3379 3389 3413 3423 3486
Size: 3379 10 24 10 63
Fragments arranged by size:
3379 63 24 10 10
BglI GCCn_nnn'nGGC
Cuts at: 0 2467 3486
Size: 2467 1019
Bme1580I G_kGCm'C
Cuts at: 0 2450 2828 3486
Size: 2450 378 658
Fragments arranged by size:
2450 658 378
BmgBI CAC'GTC
Cuts at: 0 336 2756 3486
Size: 336 2420 730
Fragments arranged by size:
2420 730 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 510 1228 1317 3486
Size: 510 718 89 2169
Fragments arranged by size:
2169 718 510 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 239 433 2055 3486
Size: 239 194 1622 1431
Fragments arranged by size:
1622 1431 239 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 742 1507 1547 1945 2976 3486
Size: 478 264 765 40 398 1031 510
Fragments arranged by size:
1031 765 510 478 398 264 40
Bpu10I CC'TnA_GC
Cuts at: 0 1075 2654 2713 2983 3486
Size: 1075 1579 59 270 503
Fragments arranged by size:
1579 1075 503 270 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 793 1173 2823 3486
Size: 234 559 380 1650 663
Fragments arranged by size:
1650 663 559 380 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 385 3486
Size: 385 3101
BsaWI w'CCGG_w
Cuts at: 0 3113 3486
Size: 3113 373
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 559 589 3486
Size: 559 30 2897
Fragments arranged by size:
2897 559 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 418 1504 2463 2563 2581 2584 2593
Size: 415 3 1086 959 100 18 3 9
Cuts at: 2593 2596 2599 2602 2605 2626 2935 3486
Size: 3 3 3 3 21 309 551
Fragments arranged by size:
1086 959 551 415 309 100 21 18
9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1264 2006 2233 2338 2520 2534 2556 3486
Size: 1264 742 227 105 182 14 22 930
Fragments arranged by size:
1264 930 742 227 182 105 22 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 490 607 1402 2530 2627 2642 3486
Size: 115 375 117 795 1128 97 15 844
Fragments arranged by size:
1128 844 795 375 117 115 97 15
BsiHKAI G_wGCw'C
Cuts at: 0 809 1332 2429 3296 3416 3486
Size: 809 523 1097 867 120 70
Fragments arranged by size:
1097 867 809 523 120 70
BsmI GAATG_Cn'
Cuts at: 0 1927 3486
Size: 1927 1559
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1692 2657 3486
Size: 1692 965 829
Fragments arranged by size:
1692 965 829
Bsp1286I G_dGCh'C
Cuts at: 0 809 1297 1332 2429 2450 2655 2828 3296
Size: 809 488 35 1097 21 205 173 468
Cuts at: 3296 3416 3486
Size: 120 70
Fragments arranged by size:
1097 809 488 468 205 173 120 70
35 21
BspEI T'CCGG_A
Cuts at: 0 3113 3486
Size: 3113 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1237 3295 3486
Size: 1237 2058 191
Fragments arranged by size:
2058 1237 191
BsrDI GCAATG_nn'
Cuts at: 0 1463 1941 3350 3486
Size: 1463 478 1409 136
Fragments arranged by size:
1463 1409 478 136
BsrFI r'CCGG_y
Cuts at: 0 3075 3486
Size: 3075 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1229 2286 2977 3486
Size: 1229 1057 691 509
Fragments arranged by size:
1229 1057 691 509
BstEII G'GTnAC_C
Cuts at: 0 775 3486
Size: 775 2711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2006 3486
Size: 2006 1480
BstYI r'GATC_y
Cuts at: 0 219 267 1317 2378 3486
Size: 219 48 1050 1061 1108
Fragments arranged by size:
1108 1061 1050 219 48
BstZ17I GTA'TAC
Cuts at: 0 860 3486
Size: 860 2626
Bsu36I CC'TnA_GG
Cuts at: 0 837 3486
Size: 837 2649
BtgI C'CryG_G
Cuts at: 0 365 482 1639 2312 2430 3486
Size: 365 117 1157 673 118 1056
Fragments arranged by size:
1157 1056 673 365 118 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1722 2138 2273 3486
Size: 1722 416 135 1213
Fragments arranged by size:
1722 1213 416 135
BtsI GCAGTG_nn'
Cuts at: 0 1136 1414 2514 3216 3254 3486
Size: 1136 278 1100 702 38 232
Fragments arranged by size:
1136 1100 702 278 232 38
EaeI y'GGCC_r
Cuts at: 0 517 1546 1579 2637 3486
Size: 517 1029 33 1058 849
Fragments arranged by size:
1058 1029 849 517 33
EarI CTCTTCn'nnn_
Cuts at: 0 116 1883 2063 2570 3486
Size: 116 1767 180 507 916
Fragments arranged by size:
1767 916 507 180 116
EcoICRI GAG'CTC
Cuts at: 0 807 2427 3294 3486
Size: 807 1620 867 192
Fragments arranged by size:
1620 867 807 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 742 1270 1507 1547 1945 2381 2976
Size: 478 264 528 237 40 398 436 595
Cuts at: 2976 3486
Size: 510
Fragments arranged by size:
595 528 510 478 436 398 264 237
40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1051 3486
Size: 1051 2435
EcoO109I rG'GnC_Cy
Cuts at: 0 476 1786 2832 3011 3045 3486
Size: 476 1310 1046 179 34 441
Fragments arranged by size:
1310 1046 476 441 179 34
EcoRI G'AATT_C
Cuts at: 0 237 3486
Size: 237 3249
HaeII r_GCGC'y
Cuts at: 0 1017 2268 3486
Size: 1017 1251 1218
Fragments arranged by size:
1251 1218 1017
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 769 801 1932 1964 2378 2410
Size: 354 32 383 32 1131 32 414 32
Cuts at: 2410 3003 3035 3194 3226 3258 3486
Size: 593 32 159 32 32 228
Fragments arranged by size:
1131 593 414 383 354 228 159 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1200 1646 3486
Size: 1200 446 1840
Fragments arranged by size:
1840 1200 446
HindIII A'AGCT_T
Cuts at: 0 1561 3486
Size: 1561 1925
HpaI GTT'AAC
Cuts at: 0 1646 3486
Size: 1646 1840
KpnI G_GTAC'C
Cuts at: 0 50 653 3486
Size: 50 603 2833
Fragments arranged by size:
2833 603 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1082 1382 1842 2860 3486
Size: 989 93 300 460 1018 626
Fragments arranged by size:
1018 989 626 460 300 93
MscI TGG'CCA
Cuts at: 0 519 1548 3486
Size: 519 1029 1938
Fragments arranged by size:
1938 1029 519
MslI CAynn'nnrTG
Cuts at: 0 334 358 877 916 2161 2246 3486
Size: 334 24 519 39 1245 85 1240
Fragments arranged by size:
1245 1240 519 334 85 39 24
MspA1I CmG'CkG
Cuts at: 0 204 1663 2342 2682 2996 3486
Size: 204 1459 679 340 314 490
Fragments arranged by size:
1459 679 490 340 314 204
NcoI C'CATG_G
Cuts at: 0 365 2312 3486
Size: 365 1947 1174
Fragments arranged by size:
1947 1174 365
NspI r_CATG'y
Cuts at: 0 516 1859 3189 3486
Size: 516 1343 1330 297
Fragments arranged by size:
1343 1330 516 297
PasI CC'CwG_GG
Cuts at: 0 2221 3486
Size: 2221 1265
PciI A'CATG_T
Cuts at: 0 512 1855 3486
Size: 512 1343 1631
Fragments arranged by size:
1631 1343 512
PflMI CCAn_nnn'nTGG
Cuts at: 0 720 3486
Size: 720 2766
PpuMI rG'GwC_Cy
Cuts at: 0 3011 3045 3486
Size: 3011 34 441
Fragments arranged by size:
3011 441 34
PshAI GACnn'nnGTC
Cuts at: 0 3212 3486
Size: 3212 274
PspXI vC'TCGA_Gb
Cuts at: 0 436 3486
Size: 436 3050
PstI C_TGCA'G
Cuts at: 0 1765 2171 2297 2459 2973 3223 3486
Size: 1765 406 126 162 514 250 263
Fragments arranged by size:
1765 514 406 263 250 162 126
PvuII CAG'CTG
Cuts at: 0 1663 2996 3486
Size: 1663 1333 490
Fragments arranged by size:
1663 1333 490
SacI G_AGCT'C
Cuts at: 0 809 2429 3296 3486
Size: 809 1620 867 190
Fragments arranged by size:
1620 867 809 190
ScaI AGT'ACT
Cuts at: 0 595 3486
Size: 595 2891
SexAI A'CCwGG_T
Cuts at: 0 3087 3486
Size: 3087 399
SfcI C'TryA_G
Cuts at: 0 707 866 1184 1284 1535 1761 2167 2293
Size: 707 159 318 100 251 226 406 126
Cuts at: 2293 2407 2455 2969 3219 3486
Size: 114 48 514 250 267
Fragments arranged by size:
707 514 406 318 267 251 250 226
159 126 114 100 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2467 3486
Size: 2467 1019
SmaI CCC'GGG
Cuts at: 0 2197 3486
Size: 2197 1289
SmlI C'TyrA_G
Cuts at: 0 213 436 808 1152 2802 3486
Size: 213 223 372 344 1650 684
Fragments arranged by size:
1650 684 372 344 223 213
StuI AGG'CCT
Cuts at: 0 1379 3143 3486
Size: 1379 1764 343
Fragments arranged by size:
1764 1379 343
StyI C'CwwG_G
Cuts at: 0 365 520 1052 2312 3486
Size: 365 155 532 1260 1174
Fragments arranged by size:
1260 1174 532 365 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1848 2169 2201 3139 3486
Size: 763 1085 321 32 938 347
Fragments arranged by size:
1085 938 763 347 321 32
TatI w'GTAC_w
Cuts at: 0 593 1022 1539 3486
Size: 593 429 517 1947
Fragments arranged by size:
1947 593 517 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1515 1547 2422 2454 3486
Size: 1309 32 174 32 875 32 1032
Fragments arranged by size:
1309 1032 875 174 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2211 3486
Size: 2211 1275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 40 640 2836 3486
Size: 40 600 2196 650
Fragments arranged by size:
2196 650 600 40
XhoI C'TCGA_G
Cuts at: 0 436 3486
Size: 436 3050
XmaI C'CCGG_G
Cuts at: 0 2195 3486
Size: 2195 1291
XmnI GAAnn'nnTTC
Cuts at: 0 20 1379 3486
Size: 20 1359 2107
Fragments arranged by size:
2107 1359 20
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PflMI PpuMI PshAI
PspXI PstI PvuII SacI ScaI SexAI SfcI SfiI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BbeI BciVI BclI
BfrBI BglII BlpI BmtI BsaAI BsaBI BsaHI BsiEI
BsiWI BspHI BsrBI BsrGI BssHII BssSI BstBI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRV FalI
FseI FspI FspAI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI ZraI