Restriction map for
pF1KSDA1014
(Linear) (Six-base) MAPSORT of: KIAA1014.seq Check: 1639 from: 1 to: 3051
KIAA1014.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2613 3051
Size: 2613 438
Acc65I G'GTAC_C
Cuts at: 0 21 3051
Size: 21 3030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1729 2071 2273 3043 3051
Size: 191 1538 342 202 770 8
Fragments arranged by size:
1538 770 342 202 191 8
AleI CACnn'nnGTG
Cuts at: 0 172 2395 3051
Size: 172 2223 656
Fragments arranged by size:
2223 656 172
AlwNI CAG_nnn'CTG
Cuts at: 0 487 799 2605 2955 3051
Size: 487 312 1806 350 96
Fragments arranged by size:
1806 487 350 312 96
ApoI r'AATT_y
Cuts at: 0 920 1470 1491 1579 1618 1977 3051
Size: 920 550 21 88 39 359 1074
Fragments arranged by size:
1074 920 550 359 88 39 21
AseI AT'TA_AT
Cuts at: 0 1637 3051
Size: 1637 1414
AvaI C'yCGr_G
Cuts at: 0 14 65 2678 3051
Size: 14 51 2613 373
Fragments arranged by size:
2613 373 51 14
BanI G'GyrC_C
Cuts at: 0 21 3051
Size: 21 3030
BanII G_rGCy'C
Cuts at: 0 1716 2249 3051
Size: 1716 533 802
Fragments arranged by size:
1716 802 533
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 3051
Size: 1145 1906
BbvCI CC'TCA_GC
Cuts at: 0 473 3051
Size: 473 2578
BciVI GTATCCnnnnn_n'
Cuts at: 0 2866 3051
Size: 2866 185
BglI GCCn_nnn'nGGC
Cuts at: 0 154 187 3051
Size: 154 33 2864
Fragments arranged by size:
2864 154 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 776 1821 3051
Size: 776 1045 1230
Fragments arranged by size:
1230 1045 776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 3051
Size: 135 2916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1240 1421 1508 2138 2414 3051
Size: 1199 41 181 87 630 276 637
Fragments arranged by size:
1199 637 630 276 181 87 41
Bpu10I CC'TnA_GC
Cuts at: 0 473 3051
Size: 473 2578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 3051
Size: 1310 1741
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1401 1431 3051
Size: 1401 30 1620
Fragments arranged by size:
1620 1401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 461 958 961 1060 1204 2165 2177 2257
Size: 461 497 3 99 144 961 12 80
Cuts at: 2257 2717 2720 2723 2726 2729 3051
Size: 460 3 3 3 3 322
Fragments arranged by size:
961 497 461 460 322 144 99 80
12 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 136 3051
Size: 136 2915
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 2903 3051
Size: 256 2647 148
Fragments arranged by size:
2647 256 148
BsiEI CG_ry'CG
Cuts at: 0 30 3051
Size: 30 3021
BsmI GAATG_Cn'
Cuts at: 0 1340 3051
Size: 1340 1711
Bsp1286I G_dGCh'C
Cuts at: 0 1716 2249 3051
Size: 1716 533 802
Fragments arranged by size:
1716 802 533
BspHI T'CATG_A
Cuts at: 0 278 3051
Size: 278 2773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2613 3051
Size: 2613 438
BsrDI GCAATG_nn'
Cuts at: 0 2659 3051
Size: 2659 392
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1462 2605 3051
Size: 1462 1143 446
Fragments arranged by size:
1462 1143 446
BstEII G'GTnAC_C
Cuts at: 0 2464 3051
Size: 2464 587
BstXI CCAn_nnnn'nTGG
Cuts at: 0 598 3051
Size: 598 2453
BstYI r'GATC_y
Cuts at: 0 1155 2207 3051
Size: 1155 1052 844
Fragments arranged by size:
1155 1052 844
Bsu36I CC'TnA_GG
Cuts at: 0 992 3002 3051
Size: 992 2010 49
Fragments arranged by size:
2010 992 49
BtgI C'CryG_G
Cuts at: 0 125 170 185 3051
Size: 125 45 15 2866
Fragments arranged by size:
2866 125 45 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 953 3051
Size: 953 2098
BtsI GCAGTG_nn'
Cuts at: 0 1170 2390 2402 3051
Size: 1170 1220 12 649
Fragments arranged by size:
1220 1170 649 12
ClaI AT'CG_AT
Cuts at: 0 455 3051
Size: 455 2596
EaeI y'GGCC_r
Cuts at: 0 27 434 3051
Size: 27 407 2617
Fragments arranged by size:
2617 407 27
EagI C'GGCC_G
Cuts at: 0 27 3051
Size: 27 3024
EarI CTCTTCn'nnn_
Cuts at: 0 1007 1190 1883 2806 2870 2894 2930 3019
Size: 1007 183 693 923 64 24 36 89
Cuts at: 3019 3051
Size: 32
Fragments arranged by size:
1007 923 693 183 89 64 36 32
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 44 3051
Size: 44 3007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1199 1240 1421 1508 1729 2071 2138
Size: 191 1008 41 181 87 221 342 67
Cuts at: 2138 2273 2414 3043 3051
Size: 135 141 629 8
Fragments arranged by size:
1008 629 342 221 191 181 141 135
87 67 41 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 209 2255 3001 3051
Size: 209 2046 746 50
Fragments arranged by size:
2046 746 209 50
EcoO109I rG'GnC_Cy
Cuts at: 0 61 87 3051
Size: 61 26 2964
Fragments arranged by size:
2964 61 26
EcoRI G'AATT_C
Cuts at: 0 920 1491 3051
Size: 920 571 1560
Fragments arranged by size:
1560 920 571
EcoRV GAT'ATC
Cuts at: 0 1308 3051
Size: 1308 1743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1228 1732 3051
Size: 1228 504 1319
Fragments arranged by size:
1319 1228 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1634 1666 1671 1703 2896 2928 3051
Size: 1634 32 5 32 1193 32 123
Fragments arranged by size:
1634 1193 123 32 32 32 5
HincII GTy'rAC
Cuts at: 0 413 3051
Size: 413 2638
KpnI G_GTAC'C
Cuts at: 0 25 3051
Size: 25 3026
MfeI C'AATT_G
Cuts at: 0 1457 2639 3051
Size: 1457 1182 412
Fragments arranged by size:
1457 1182 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 618 1106 1679 3051
Size: 535 83 488 573 1372
Fragments arranged by size:
1372 573 535 488 83
MscI TGG'CCA
Cuts at: 0 436 3051
Size: 436 2615
MslI CAynn'nnrTG
Cuts at: 0 172 2395 3051
Size: 172 2223 656
Fragments arranged by size:
2223 656 172
MspA1I CmG'CkG
Cuts at: 0 127 172 187 298 2955 3051
Size: 127 45 15 111 2657 96
Fragments arranged by size:
2657 127 111 96 45 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1444 3051
Size: 1444 1607
PfoI T'CCnGG_A
Cuts at: 0 2210 3051
Size: 2210 841
PpuMI rG'GwC_Cy
Cuts at: 0 61 87 3051
Size: 61 26 2964
Fragments arranged by size:
2964 61 26
PstI C_TGCA'G
Cuts at: 0 483 2397 2576 3051
Size: 483 1914 179 475
Fragments arranged by size:
1914 483 475 179
PvuII CAG'CTG
Cuts at: 0 298 2955 3051
Size: 298 2657 96
Fragments arranged by size:
2657 298 96
SacII CC_GC'GG
Cuts at: 0 128 173 188 3051
Size: 128 45 15 2863
Fragments arranged by size:
2863 128 45 15
SanDI GG'GwC_CC
Cuts at: 0 87 3051
Size: 87 2964
SfcI C'TryA_G
Cuts at: 0 479 488 2378 2393 2439 2453 2459 2572
Size: 479 9 1890 15 46 14 6 113
Cuts at: 2572 3051
Size: 479
Fragments arranged by size:
1890 479 479 113 46 15 14 9
6
SmaI CCC'GGG
Cuts at: 0 16 3051
Size: 16 3035
SmlI C'TyrA_G
Cuts at: 0 1325 3051
Size: 1325 1726
SpeI A'CTAG_T
Cuts at: 0 805 3051
Size: 805 2246
StyI C'CwwG_G
Cuts at: 0 1259 2123 2586 3051
Size: 1259 864 463 465
Fragments arranged by size:
1259 864 465 463
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 3051
Size: 604 2447
TatI w'GTAC_w
Cuts at: 0 427 1075 1539 3051
Size: 427 648 464 1512
Fragments arranged by size:
1512 648 464 427
XmaI C'CCGG_G
Cuts at: 0 14 3051
Size: 14 3037
XmnI GAAnn'nnTTC
Cuts at: 0 920 2996 3051
Size: 920 2076 55
Fragments arranged by size:
2076 920 55
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApoI AseI AvaI
BanI BanII BbsI BbvCI BciVI BglI BmrI BplI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I
PflMI PfoI PpuMI PstI PvuII SacII SanDI SfcI
SmaI SmlI SpeI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I CspCI DraI DraIII DrdI EcoICRI FseI
FspI FspAI HaeII HindIII HpaI KasI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI