(Linear) (Six-base) MAPSORT of: KIAA1014.seq Check: 1639 from: 1 to: 3051 KIAA1014.orf With 182 enzymes: SgfI * October 24, 2006 15:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2613 3051 Size: 2613 438 Acc65I G'GTAC_C Cuts at: 0 21 3051 Size: 21 3030 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 191 1729 2071 2273 3043 3051 Size: 191 1538 342 202 770 8 Fragments arranged by size: 1538 770 342 202 191 8 AleI CACnn'nnGTG Cuts at: 0 172 2395 3051 Size: 172 2223 656 Fragments arranged by size: 2223 656 172 AlwNI CAG_nnn'CTG Cuts at: 0 487 799 2605 2955 3051 Size: 487 312 1806 350 96 Fragments arranged by size: 1806 487 350 312 96 ApoI r'AATT_y Cuts at: 0 920 1470 1491 1579 1618 1977 3051 Size: 920 550 21 88 39 359 1074 Fragments arranged by size: 1074 920 550 359 88 39 21 AseI AT'TA_AT Cuts at: 0 1637 3051 Size: 1637 1414 AvaI C'yCGr_G Cuts at: 0 14 65 2678 3051 Size: 14 51 2613 373 Fragments arranged by size: 2613 373 51 14 BanI G'GyrC_C Cuts at: 0 21 3051 Size: 21 3030 BanII G_rGCy'C Cuts at: 0 1716 2249 3051 Size: 1716 533 802 Fragments arranged by size: 1716 802 533 BbsI GAAGACnn'nnnn_ Cuts at: 0 1145 3051 Size: 1145 1906 BbvCI CC'TCA_GC Cuts at: 0 473 3051 Size: 473 2578 BciVI GTATCCnnnnn_n' Cuts at: 0 2866 3051 Size: 2866 185 BglI GCCn_nnn'nGGC Cuts at: 0 154 187 3051 Size: 154 33 2864 Fragments arranged by size: 2864 154 33 BmrI ACTGGGnnnn_n' Cuts at: 0 776 1821 3051 Size: 776 1045 1230 Fragments arranged by size: 1230 1045 776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 135 3051 Size: 135 2916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1199 1240 1421 1508 2138 2414 3051 Size: 1199 41 181 87 630 276 637 Fragments arranged by size: 1199 637 630 276 181 87 41 Bpu10I CC'TnA_GC Cuts at: 0 473 3051 Size: 473 2578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1310 3051 Size: 1310 1741 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1401 1431 3051 Size: 1401 30 1620 Fragments arranged by size: 1620 1401 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 461 958 961 1060 1204 2165 2177 2257 Size: 461 497 3 99 144 961 12 80 Cuts at: 2257 2717 2720 2723 2726 2729 3051 Size: 460 3 3 3 3 322 Fragments arranged by size: 961 497 461 460 322 144 99 80 12 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 136 3051 Size: 136 2915 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 2903 3051 Size: 256 2647 148 Fragments arranged by size: 2647 256 148 BsiEI CG_ry'CG Cuts at: 0 30 3051 Size: 30 3021 BsmI GAATG_Cn' Cuts at: 0 1340 3051 Size: 1340 1711 Bsp1286I G_dGCh'C Cuts at: 0 1716 2249 3051 Size: 1716 533 802 Fragments arranged by size: 1716 802 533 BspHI T'CATG_A Cuts at: 0 278 3051 Size: 278 2773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2613 3051 Size: 2613 438 BsrDI GCAATG_nn' Cuts at: 0 2659 3051 Size: 2659 392 BstAPI GCAn_nnn'nTGC Cuts at: 0 1462 2605 3051 Size: 1462 1143 446 Fragments arranged by size: 1462 1143 446 BstEII G'GTnAC_C Cuts at: 0 2464 3051 Size: 2464 587 BstXI CCAn_nnnn'nTGG Cuts at: 0 598 3051 Size: 598 2453 BstYI r'GATC_y Cuts at: 0 1155 2207 3051 Size: 1155 1052 844 Fragments arranged by size: 1155 1052 844 Bsu36I CC'TnA_GG Cuts at: 0 992 3002 3051 Size: 992 2010 49 Fragments arranged by size: 2010 992 49 BtgI C'CryG_G Cuts at: 0 125 170 185 3051 Size: 125 45 15 2866 Fragments arranged by size: 2866 125 45 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 953 3051 Size: 953 2098 BtsI GCAGTG_nn' Cuts at: 0 1170 2390 2402 3051 Size: 1170 1220 12 649 Fragments arranged by size: 1220 1170 649 12 ClaI AT'CG_AT Cuts at: 0 455 3051 Size: 455 2596 EaeI y'GGCC_r Cuts at: 0 27 434 3051 Size: 27 407 2617 Fragments arranged by size: 2617 407 27 EagI C'GGCC_G Cuts at: 0 27 3051 Size: 27 3024 EarI CTCTTCn'nnn_ Cuts at: 0 1007 1190 1883 2806 2870 2894 2930 3019 Size: 1007 183 693 923 64 24 36 89 Cuts at: 3019 3051 Size: 32 Fragments arranged by size: 1007 923 693 183 89 64 36 32 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 44 3051 Size: 44 3007 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 191 1199 1240 1421 1508 1729 2071 2138 Size: 191 1008 41 181 87 221 342 67 Cuts at: 2138 2273 2414 3043 3051 Size: 135 141 629 8 Fragments arranged by size: 1008 629 342 221 191 181 141 135 87 67 41 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 209 2255 3001 3051 Size: 209 2046 746 50 Fragments arranged by size: 2046 746 209 50 EcoO109I rG'GnC_Cy Cuts at: 0 61 87 3051 Size: 61 26 2964 Fragments arranged by size: 2964 61 26 EcoRI G'AATT_C Cuts at: 0 920 1491 3051 Size: 920 571 1560 Fragments arranged by size: 1560 920 571 EcoRV GAT'ATC Cuts at: 0 1308 3051 Size: 1308 1743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1228 1732 3051 Size: 1228 504 1319 Fragments arranged by size: 1319 1228 504 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1634 1666 1671 1703 2896 2928 3051 Size: 1634 32 5 32 1193 32 123 Fragments arranged by size: 1634 1193 123 32 32 32 5 HincII GTy'rAC Cuts at: 0 413 3051 Size: 413 2638 KpnI G_GTAC'C Cuts at: 0 25 3051 Size: 25 3026 MfeI C'AATT_G Cuts at: 0 1457 2639 3051 Size: 1457 1182 412 Fragments arranged by size: 1457 1182 412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 535 618 1106 1679 3051 Size: 535 83 488 573 1372 Fragments arranged by size: 1372 573 535 488 83 MscI TGG'CCA Cuts at: 0 436 3051 Size: 436 2615 MslI CAynn'nnrTG Cuts at: 0 172 2395 3051 Size: 172 2223 656 Fragments arranged by size: 2223 656 172 MspA1I CmG'CkG Cuts at: 0 127 172 187 298 2955 3051 Size: 127 45 15 111 2657 96 Fragments arranged by size: 2657 127 111 96 45 15 PflMI CCAn_nnn'nTGG Cuts at: 0 1444 3051 Size: 1444 1607 PfoI T'CCnGG_A Cuts at: 0 2210 3051 Size: 2210 841 PpuMI rG'GwC_Cy Cuts at: 0 61 87 3051 Size: 61 26 2964 Fragments arranged by size: 2964 61 26 PstI C_TGCA'G Cuts at: 0 483 2397 2576 3051 Size: 483 1914 179 475 Fragments arranged by size: 1914 483 475 179 PvuII CAG'CTG Cuts at: 0 298 2955 3051 Size: 298 2657 96 Fragments arranged by size: 2657 298 96 SacII CC_GC'GG Cuts at: 0 128 173 188 3051 Size: 128 45 15 2863 Fragments arranged by size: 2863 128 45 15 SanDI GG'GwC_CC Cuts at: 0 87 3051 Size: 87 2964 SfcI C'TryA_G Cuts at: 0 479 488 2378 2393 2439 2453 2459 2572 Size: 479 9 1890 15 46 14 6 113 Cuts at: 2572 3051 Size: 479 Fragments arranged by size: 1890 479 479 113 46 15 14 9 6 SmaI CCC'GGG Cuts at: 0 16 3051 Size: 16 3035 SmlI C'TyrA_G Cuts at: 0 1325 3051 Size: 1325 1726 SpeI A'CTAG_T Cuts at: 0 805 3051 Size: 805 2246 StyI C'CwwG_G Cuts at: 0 1259 2123 2586 3051 Size: 1259 864 463 465 Fragments arranged by size: 1259 864 465 463 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 604 3051 Size: 604 2447 TatI w'GTAC_w Cuts at: 0 427 1075 1539 3051 Size: 427 648 464 1512 Fragments arranged by size: 1512 648 464 427 XmaI C'CCGG_G Cuts at: 0 14 3051 Size: 14 3037 XmnI GAAnn'nnTTC Cuts at: 0 920 2996 3051 Size: 920 2076 55 Fragments arranged by size: 2076 920 55 Enzymes that do cut: AarI Acc65I AcuI AleI AlwNI ApoI AseI AvaI BanI BanII BbsI BbvCI BciVI BglI BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I PflMI PfoI PpuMI PstI PvuII SacII SanDI SfcI SmaI SmlI SpeI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I CspCI DraI DraIII DrdI EcoICRI FseI FspI FspAI HaeII HindIII HpaI KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI