Restriction map for pF1KSDA1014
 (Linear) (Six-base) MAPSORT of: KIAA1014.seq  Check: 1639  from: 1  to: 3051



KIAA1014.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2613    3051 
   Size:       2613     438
 
Acc65I G'GTAC_C

Cuts at:      0      21    3051 
   Size:         21    3030
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1729    2071    2273    3043    3051 
   Size:        191    1538     342     202     770       8

  Fragments arranged by size: 

                1538     770     342     202     191       8
 
AleI CACnn'nnGTG

Cuts at:      0     172    2395    3051 
   Size:        172    2223     656

  Fragments arranged by size: 

                2223     656     172
 
AlwNI CAG_nnn'CTG

Cuts at:      0     487     799    2605    2955    3051 
   Size:        487     312    1806     350      96

  Fragments arranged by size: 

                1806     487     350     312      96
 
ApoI r'AATT_y

Cuts at:      0     920    1470    1491    1579    1618    1977    3051 
   Size:        920     550      21      88      39     359    1074

  Fragments arranged by size: 

                1074     920     550     359      88      39      21
 
AseI AT'TA_AT

Cuts at:      0    1637    3051 
   Size:       1637    1414
 
AvaI C'yCGr_G

Cuts at:      0      14      65    2678    3051 
   Size:         14      51    2613     373

  Fragments arranged by size: 

                2613     373      51      14
 
BanI G'GyrC_C

Cuts at:      0      21    3051 
   Size:         21    3030
 
BanII G_rGCy'C

Cuts at:      0    1716    2249    3051 
   Size:       1716     533     802

  Fragments arranged by size: 

                1716     802     533
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1145    3051 
   Size:       1145    1906
 
BbvCI CC'TCA_GC

Cuts at:      0     473    3051 
   Size:        473    2578
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2866    3051 
   Size:       2866     185
 
BglI GCCn_nnn'nGGC

Cuts at:      0     154     187    3051 
   Size:        154      33    2864

  Fragments arranged by size: 

                2864     154      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     776    1821    3051 
   Size:        776    1045    1230

  Fragments arranged by size: 

                1230    1045     776
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     135    3051 
   Size:        135    2916
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1199    1240    1421    1508    2138    2414    3051 
   Size:       1199      41     181      87     630     276     637

  Fragments arranged by size: 

                1199     637     630     276     181      87      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     473    3051 
   Size:        473    2578
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    3051 
   Size:       1310    1741
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1401    1431    3051 
   Size:       1401      30    1620

  Fragments arranged by size: 

                1620    1401      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     461     958     961    1060    1204    2165    2177    2257 
   Size:        461     497       3      99     144     961      12      80

Cuts at:   2257    2717    2720    2723    2726    2729    3051 
   Size:        460       3       3       3       3     322

  Fragments arranged by size: 

                 961     497     461     460     322     144      99      80
                  12       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     136    3051 
   Size:        136    2915
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    2903    3051 
   Size:        256    2647     148

  Fragments arranged by size: 

                2647     256     148
 
BsiEI CG_ry'CG

Cuts at:      0      30    3051 
   Size:         30    3021
 
BsmI GAATG_Cn'

Cuts at:      0    1340    3051 
   Size:       1340    1711
 
Bsp1286I G_dGCh'C

Cuts at:      0    1716    2249    3051 
   Size:       1716     533     802

  Fragments arranged by size: 

                1716     802     533
 
BspHI T'CATG_A

Cuts at:      0     278    3051 
   Size:        278    2773
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2613    3051 
   Size:       2613     438
 
BsrDI GCAATG_nn'

Cuts at:      0    2659    3051 
   Size:       2659     392
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1462    2605    3051 
   Size:       1462    1143     446

  Fragments arranged by size: 

                1462    1143     446
 
BstEII G'GTnAC_C

Cuts at:      0    2464    3051 
   Size:       2464     587
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     598    3051 
   Size:        598    2453
 
BstYI r'GATC_y

Cuts at:      0    1155    2207    3051 
   Size:       1155    1052     844

  Fragments arranged by size: 

                1155    1052     844
 
Bsu36I CC'TnA_GG

Cuts at:      0     992    3002    3051 
   Size:        992    2010      49

  Fragments arranged by size: 

                2010     992      49
 
BtgI C'CryG_G

Cuts at:      0     125     170     185    3051 
   Size:        125      45      15    2866

  Fragments arranged by size: 

                2866     125      45      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     953    3051 
   Size:        953    2098
 
BtsI GCAGTG_nn'

Cuts at:      0    1170    2390    2402    3051 
   Size:       1170    1220      12     649

  Fragments arranged by size: 

                1220    1170     649      12
 
ClaI AT'CG_AT

Cuts at:      0     455    3051 
   Size:        455    2596
 
EaeI y'GGCC_r

Cuts at:      0      27     434    3051 
   Size:         27     407    2617

  Fragments arranged by size: 

                2617     407      27
 
EagI C'GGCC_G

Cuts at:      0      27    3051 
   Size:         27    3024
 
EarI CTCTTCn'nnn_

Cuts at:      0    1007    1190    1883    2806    2870    2894    2930    3019 
   Size:       1007     183     693     923      64      24      36      89

Cuts at:   3019    3051 
   Size:         32

  Fragments arranged by size: 

                1007     923     693     183      89      64      36      32
                  24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      44    3051 
   Size:         44    3007
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1199    1240    1421    1508    1729    2071    2138 
   Size:        191    1008      41     181      87     221     342      67

Cuts at:   2138    2273    2414    3043    3051 
   Size:        135     141     629       8

  Fragments arranged by size: 

                1008     629     342     221     191     181     141     135
                  87      67      41       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     209    2255    3001    3051 
   Size:        209    2046     746      50

  Fragments arranged by size: 

                2046     746     209      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0      61      87    3051 
   Size:         61      26    2964

  Fragments arranged by size: 

                2964      61      26
 
EcoRI G'AATT_C

Cuts at:      0     920    1491    3051 
   Size:        920     571    1560

  Fragments arranged by size: 

                1560     920     571
 
EcoRV GAT'ATC

Cuts at:      0    1308    3051 
   Size:       1308    1743
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1228    1732    3051 
   Size:       1228     504    1319

  Fragments arranged by size: 

                1319    1228     504
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1634    1666    1671    1703    2896    2928    3051 
   Size:       1634      32       5      32    1193      32     123

  Fragments arranged by size: 

                1634    1193     123      32      32      32       5
 
HincII GTy'rAC

Cuts at:      0     413    3051 
   Size:        413    2638
 
KpnI G_GTAC'C

Cuts at:      0      25    3051 
   Size:         25    3026
 
MfeI C'AATT_G

Cuts at:      0    1457    2639    3051 
   Size:       1457    1182     412

  Fragments arranged by size: 

                1457    1182     412
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     618    1106    1679    3051 
   Size:        535      83     488     573    1372

  Fragments arranged by size: 

                1372     573     535     488      83
 
MscI TGG'CCA

Cuts at:      0     436    3051 
   Size:        436    2615
 
MslI CAynn'nnrTG

Cuts at:      0     172    2395    3051 
   Size:        172    2223     656

  Fragments arranged by size: 

                2223     656     172
 
MspA1I CmG'CkG

Cuts at:      0     127     172     187     298    2955    3051 
   Size:        127      45      15     111    2657      96

  Fragments arranged by size: 

                2657     127     111      96      45      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1444    3051 
   Size:       1444    1607
 
PfoI T'CCnGG_A

Cuts at:      0    2210    3051 
   Size:       2210     841
 
PpuMI rG'GwC_Cy

Cuts at:      0      61      87    3051 
   Size:         61      26    2964

  Fragments arranged by size: 

                2964      61      26
 
PstI C_TGCA'G

Cuts at:      0     483    2397    2576    3051 
   Size:        483    1914     179     475

  Fragments arranged by size: 

                1914     483     475     179
 
PvuII CAG'CTG

Cuts at:      0     298    2955    3051 
   Size:        298    2657      96

  Fragments arranged by size: 

                2657     298      96
 
SacII CC_GC'GG

Cuts at:      0     128     173     188    3051 
   Size:        128      45      15    2863

  Fragments arranged by size: 

                2863     128      45      15
 
SanDI GG'GwC_CC

Cuts at:      0      87    3051 
   Size:         87    2964
 
SfcI C'TryA_G

Cuts at:      0     479     488    2378    2393    2439    2453    2459    2572 
   Size:        479       9    1890      15      46      14       6     113

Cuts at:   2572    3051 
   Size:        479

  Fragments arranged by size: 

                1890     479     479     113      46      15      14       9
                   6
 
SmaI CCC'GGG

Cuts at:      0      16    3051 
   Size:         16    3035
 
SmlI C'TyrA_G

Cuts at:      0    1325    3051 
   Size:       1325    1726
 
SpeI A'CTAG_T

Cuts at:      0     805    3051 
   Size:        805    2246
 
StyI C'CwwG_G

Cuts at:      0    1259    2123    2586    3051 
   Size:       1259     864     463     465

  Fragments arranged by size: 

                1259     864     465     463
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     604    3051 
   Size:        604    2447
 
TatI w'GTAC_w

Cuts at:      0     427    1075    1539    3051 
   Size:        427     648     464    1512

  Fragments arranged by size: 

                1512     648     464     427
 
XmaI C'CCGG_G

Cuts at:      0      14    3051 
   Size:         14    3037
 
XmnI GAAnn'nnTTC

Cuts at:      0     920    2996    3051 
   Size:        920    2076      55

  Fragments arranged by size: 

                2076     920      55
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    AlwNI     ApoI     AseI     AvaI
     BanI    BanII     BbsI    BbvCI    BciVI     BglI     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI    BsiEI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I   HincII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
    PflMI     PfoI    PpuMI     PstI    PvuII    SacII    SanDI     SfcI
     SmaI     SmlI     SpeI     StyI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I    CspCI     DraI   DraIII     DrdI  EcoICRI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI
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