Restriction map for
pF1KSDA1020
(Linear) (Six-base) MAPSORT of: KIAA1020.seq Check: 9577 from: 1 to: 1845
KIAA1020.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1829 1845
Size: 1829 16
AatII G_ACGT'C
Cuts at: 0 143 1845
Size: 143 1702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1693 1845
Size: 330 1363 152
Fragments arranged by size:
1363 330 152
AflIII A'CryG_T
Cuts at: 0 1274 1645 1845
Size: 1274 371 200
Fragments arranged by size:
1274 371 200
AleI CACnn'nnGTG
Cuts at: 0 24 1739 1845
Size: 24 1715 106
Fragments arranged by size:
1715 106 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 417 1388 1845
Size: 331 86 971 457
Fragments arranged by size:
971 457 331 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1390 1422 1845
Size: 1390 32 423
Fragments arranged by size:
1390 423 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 492 732 1845
Size: 96 396 240 1113
Fragments arranged by size:
1113 396 240 96
ApaI G_GGCC'C
Cuts at: 0 14 62 177 555 1070 1160 1845
Size: 14 48 115 378 515 90 685
Fragments arranged by size:
685 515 378 115 90 48 14
ApaLI G'TGCA_C
Cuts at: 0 1744 1845
Size: 1744 101
AvaI C'yCGr_G
Cuts at: 0 385 1083 1749 1845
Size: 385 698 666 96
Fragments arranged by size:
698 666 385 96
BanI G'GyrC_C
Cuts at: 0 898 960 1447 1636 1845
Size: 898 62 487 189 209
Fragments arranged by size:
898 487 209 189 62
BanII G_rGCy'C
Cuts at: 0 14 62 177 341 533 555 572 914
Size: 14 48 115 164 192 22 17 342
Cuts at: 914 1070 1160 1172 1505 1845
Size: 156 90 12 333 340
Fragments arranged by size:
342 340 333 192 164 156 115 90
48 22 17 14 12
BbeI G_GCGC'C
Cuts at: 0 964 1845
Size: 964 881
BbsI GAAGACnn'nnnn_
Cuts at: 0 1226 1540 1579 1845
Size: 1226 314 39 266
Fragments arranged by size:
1226 314 266 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 1016 1845
Size: 982 34 829
Fragments arranged by size:
982 829 34
BglI GCCn_nnn'nGGC
Cuts at: 0 662 833 969 1495 1667 1845
Size: 662 171 136 526 172 178
Fragments arranged by size:
662 526 178 172 171 136
BglII A'GATC_T
Cuts at: 0 284 1845
Size: 284 1561
BlpI GC'TnA_GC
Cuts at: 0 817 1499 1845
Size: 817 682 346
Fragments arranged by size:
817 682 346
Bme1580I G_kGCm'C
Cuts at: 0 14 62 177 555 901 1070 1160 1748
Size: 14 48 115 378 346 169 90 588
Cuts at: 1748 1845
Size: 97
Fragments arranged by size:
588 378 346 169 115 97 90 48
14
BmrI ACTGGGnnnn_n'
Cuts at: 0 1475 1845
Size: 1475 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 943 976 1845
Size: 361 582 33 869
Fragments arranged by size:
869 582 361 33
Bpu10I CC'TnA_GC
Cuts at: 0 946 1845
Size: 946 899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1845
Size: 400 1445
BsaAI yAC'GTr
Cuts at: 0 1380 1845
Size: 1380 465
BsaHI Gr'CG_yC
Cuts at: 0 140 961 1845
Size: 140 821 884
Fragments arranged by size:
884 821 140
BsaWI w'CCGG_w
Cuts at: 0 23 1845
Size: 23 1822
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1105 1204 1207 1210 1468 1471 1845
Size: 346 759 99 3 3 258 3 374
Fragments arranged by size:
759 374 346 258 99 3 3 3
BseYI C'CCAG_C
Cuts at: 0 76 319 657 732 812 891 1354 1548
Size: 76 243 338 75 80 79 463 194
Cuts at: 1548 1796 1845
Size: 248 49
Fragments arranged by size:
463 338 248 243 194 80 79 76
75 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1845
Size: 211 1634
BsiHKAI G_wGCw'C
Cuts at: 0 572 1736 1748 1845
Size: 572 1164 12 97
Fragments arranged by size:
1164 572 97 12
BsmI GAATG_Cn'
Cuts at: 0 1331 1845
Size: 1331 514
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1291 1845
Size: 1291 554
Bsp1286I G_dGCh'C
Cuts at: 0 14 62 177 341 533 555 572 901
Size: 14 48 115 164 192 22 17 329
Cuts at: 901 914 1070 1160 1172 1505 1736 1748 1845
Size: 13 156 90 12 333 231 12 97
Fragments arranged by size:
333 329 231 192 164 156 115 97
90 48 22 17 14 13 12 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1829 1845
Size: 1829 16
BsrBI CCG'CTC
Cuts at: 0 1053 1235 1845
Size: 1053 182 610
Fragments arranged by size:
1053 610 182
BsrFI r'CCGG_y
Cuts at: 0 424 1280 1845
Size: 424 856 565
Fragments arranged by size:
856 565 424
BssSI C'ACGA_G
Cuts at: 0 532 1567 1845
Size: 532 1035 278
Fragments arranged by size:
1035 532 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 1337 1483 1845
Size: 96 1241 146 362
Fragments arranged by size:
1241 362 146 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 921 1845
Size: 465 456 924
Fragments arranged by size:
924 465 456
BstYI r'GATC_y
Cuts at: 0 284 918 1845
Size: 284 634 927
Fragments arranged by size:
927 634 284
BtgI C'CryG_G
Cuts at: 0 497 914 1845
Size: 497 417 931
Fragments arranged by size:
931 497 417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1703 1845
Size: 1703 142
BtsI GCAGTG_nn'
Cuts at: 0 359 1446 1845
Size: 359 1087 399
Fragments arranged by size:
1087 399 359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1593 1628 1845
Size: 1593 35 217
Fragments arranged by size:
1593 217 35
DraIII CAC_nnn'GTG
Cuts at: 0 1483 1845
Size: 1483 362
EaeI y'GGCC_r
Cuts at: 0 1254 1845
Size: 1254 591
EarI CTCTTCn'nnn_
Cuts at: 0 976 1394 1412 1845
Size: 976 418 18 433
Fragments arranged by size:
976 433 418 18
EcoICRI GAG'CTC
Cuts at: 0 570 1845
Size: 570 1275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 361 943 976 1693 1845
Size: 330 31 582 33 717 152
Fragments arranged by size:
717 582 330 152 33 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1845
Size: 955 890
EcoO109I rG'GnC_Cy
Cuts at: 0 11 58 551 784 1066 1075 1157 1424
Size: 11 47 493 233 282 9 82 267
Cuts at: 1424 1467 1545 1845
Size: 43 78 300
Fragments arranged by size:
493 300 282 267 233 82 78 47
43 11 9
FspI TGC'GCA
Cuts at: 0 1229 1376 1825 1845
Size: 1229 147 449 20
Fragments arranged by size:
1229 449 147 20
FspAI rTGC'GCAy
Cuts at: 0 1376 1845
Size: 1376 469
HaeII r_GCGC'y
Cuts at: 0 964 1268 1845
Size: 964 304 577
Fragments arranged by size:
964 577 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1288 1320 1456 1488 1845
Size: 1288 32 136 32 357
Fragments arranged by size:
1288 357 136 32 32
KasI G'GCGC_C
Cuts at: 0 960 1845
Size: 960 885
MluI A'CGCG_T
Cuts at: 0 1645 1845
Size: 1645 200
MslI CAynn'nnrTG
Cuts at: 0 24 148 151 256 463 1739 1741 1845
Size: 24 124 3 105 207 1276 2 104
Fragments arranged by size:
1276 207 124 105 104 24 3 2
MspA1I CmG'CkG
Cuts at: 0 105 381 489 661 713 816 1253 1776
Size: 105 276 108 172 52 103 437 523
Cuts at: 1776 1845
Size: 69
Fragments arranged by size:
523 437 276 172 108 105 103 69
52
NaeI GCC'GGC
Cuts at: 0 426 1845
Size: 426 1419
NarI GG'CG_CC
Cuts at: 0 961 1845
Size: 961 884
NcoI C'CATG_G
Cuts at: 0 914 1845
Size: 914 931
NgoMIV G'CCGG_C
Cuts at: 0 424 1845
Size: 424 1421
NspI r_CATG'y
Cuts at: 0 1278 1656 1704 1740 1830 1845
Size: 1278 378 48 36 90 15
Fragments arranged by size:
1278 378 90 48 36 15
PciI A'CATG_T
Cuts at: 0 1274 1845
Size: 1274 571
PmlI CAC'GTG
Cuts at: 0 1380 1845
Size: 1380 465
PpuMI rG'GwC_Cy
Cuts at: 0 784 1075 1467 1545 1845
Size: 784 291 392 78 300
Fragments arranged by size:
784 392 300 291 78
PspOMI G'GGCC_C
Cuts at: 0 10 58 173 551 1066 1156 1845
Size: 10 48 115 378 515 90 689
Fragments arranged by size:
689 515 378 115 90 48 10
PstI C_TGCA'G
Cuts at: 0 104 691 1845
Size: 104 587 1154
Fragments arranged by size:
1154 587 104
PvuII CAG'CTG
Cuts at: 0 105 381 661 816 1776 1845
Size: 105 276 280 155 960 69
Fragments arranged by size:
960 280 276 155 105 69
SacI G_AGCT'C
Cuts at: 0 572 1845
Size: 572 1273
SanDI GG'GwC_CC
Cuts at: 0 1075 1845
Size: 1075 770
SapI GCTCTTCn'nnn_
Cuts at: 0 976 1394 1845
Size: 976 418 451
Fragments arranged by size:
976 451 418
SfcI C'TryA_G
Cuts at: 0 100 687 1845
Size: 100 587 1158
Fragments arranged by size:
1158 587 100
SfoI GGC'GCC
Cuts at: 0 962 1845
Size: 962 883
SmaI CCC'GGG
Cuts at: 0 1085 1845
Size: 1085 760
SmlI C'TyrA_G
Cuts at: 0 415 1845
Size: 415 1430
SphI G_CATG'C
Cuts at: 0 1704 1830 1845
Size: 1704 126 15
Fragments arranged by size:
1704 126 15
StyI C'CwwG_G
Cuts at: 0 14 122 914 1070 1343 1845
Size: 14 108 792 156 273 502
Fragments arranged by size:
792 502 273 156 108 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1494 1512 1810 1845
Size: 1494 18 298 35
Fragments arranged by size:
1494 298 35 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 768 800 1580 1612 1845
Size: 768 32 780 32 233
Fragments arranged by size:
780 768 233 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 259 1302 1845
Size: 259 1043 543
Fragments arranged by size:
1043 543 259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1128 1845
Size: 1128 717
XmaI C'CCGG_G
Cuts at: 0 1083 1845
Size: 1083 762
XmnI GAAnn'nnTTC
Cuts at: 0 602 1678 1845
Size: 602 1076 167
Fragments arranged by size:
1076 602 167
ZraI GAC'GTC
Cuts at: 0 141 1845
Size: 141 1704
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I
KasI MluI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmlI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI SfoI SmaI SmlI SphI StyI
TaqII TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BmgBI BmtI BplI BsaI BsaBI BsaXI
BsiEI BsiWI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI MmeI MscI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TatI XbaI XhoI